Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,011,138 T→A 22.0% I368I (ATT→ATA)  fliI → flagellum‑specific ATP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,011,1380TA22.0% 862.2 / 248.6 362I368I (ATT→ATA) fliIflagellum‑specific ATP synthase
Reads supporting (aligned to +/- strand):  ref base T (118/164);  new base A (42/38);  total (160/202)
Fisher's exact test for biased strand distribution p-value = 9.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 365 total aligned reads displayed.

GGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCT  >  CP009273/2010989‑2011287
                                                                                                                                                     |                                                                                                                                                     
ggcggcTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATAtt                                                                                                                                                       >  2:1829213/1‑150 (MQ=255)
  cggcTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGa                                                                                                                                                     >  1:2850572/1‑150 (MQ=255)
    gcTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAg                                                                                                                                                   <  2:2918410/150‑1 (MQ=255)
     cTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGc                                                                                                                                                  >  1:1622991/1‑150 (MQ=255)
     cTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGc                                                                                                                                                  <  1:1491124/150‑1 (MQ=255)
       cGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGt                                                                                                                                                >  2:474373/1‑150 (MQ=255)
        gATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTc                                                                                                                                               <  2:1182256/150‑1 (MQ=255)
        gATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTc                                                                                                                                               <  2:3643928/150‑1 (MQ=255)
        gATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTc                                                                                                                                               >  1:1970232/1‑150 (MQ=255)
          ttACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGa                                                                                                                                             >  2:3796127/1‑150 (MQ=255)
           tACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGAt                                                                                                                                            >  2:440619/1‑150 (MQ=255)
           tACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGAt                                                                                                                                            >  1:343701/1‑150 (MQ=255)
             ccGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCa                                                                                                                                          >  1:296781/1‑150 (MQ=255)
             ccGCGTTTTAGACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCa                                                                                                                                          <  1:2424038/150‑1 (MQ=255)
              cgcgTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAg                                                                                                                                         <  2:3164837/150‑1 (MQ=255)
                 gTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCcg                                                                                                                                      >  2:1616449/1‑150 (MQ=255)
                 gTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCcg                                                                                                                                      <  1:1070192/150‑1 (MQ=255)
                   tttATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCcgcg                                                                                                                                    <  2:1330650/150‑1 (MQ=255)
                      ataCCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCaa                                                                                                                                 >  1:1980234/1‑150 (MQ=255)
                       taCCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAAt                                                                                                                                <  1:2204182/150‑1 (MQ=255)
                       taCCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAAt                                                                                                                                >  2:2626321/1‑150 (MQ=255)
                       taCCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAAt                                                                                                                                >  2:2624076/1‑150 (MQ=255)
                          cGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGAc                                                                                                                             <  1:611891/150‑1 (MQ=255)
                            tGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACgg                                                                                                                           <  1:2614968/150‑1 (MQ=255)
                             gCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGc                                                                                                                          <  2:2092663/150‑1 (MQ=255)
                               tCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGCGt                                                                                                                        >  1:2132487/1‑150 (MQ=255)
                                cACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGtt                                                                                                                       >  1:2534293/1‑150 (MQ=255)
                                cACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGtt                                                                                                                       <  2:3940/150‑1 (MQ=255)
                                cACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGCGtt                                                                                                                       >  1:4020146/1‑150 (MQ=255)
                                cACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGCGtt                                                                                                                       >  1:1560198/1‑150 (MQ=255)
                                  cTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGa                                                                                                                     <  1:4397660/150‑1 (MQ=255)
                                   tGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGAt                                                                                                                    <  2:536294/150‑1 (MQ=255)
                                   tGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGAt                                                                                                                    <  1:538268/150‑1 (MQ=255)
                                    gAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATc                                                                                                                   >  2:2264130/1‑150 (MQ=255)
                                    gAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATc                                                                                                                   <  1:3758566/150‑1 (MQ=255)
                                    gAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATc                                                                                                                   <  1:4691693/150‑1 (MQ=255)
                                    gAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGAACCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATc                                                                                                                   >  2:4816274/1‑150 (MQ=255)
                                     aaGGCGATGACCAGCAGGATCCGAGTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCa                                                                                                                  <  1:1855510/150‑1 (MQ=255)
                                       ggCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGt                                                                                                                >  2:3465687/1‑150 (MQ=255)
                                           aTGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGc                                                                                                            <  1:720943/150‑1 (MQ=255)
                                           aTGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGc                                                                                                            >  1:4386184/1‑150 (MQ=255)
                                              aCCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAAc                                                                                                         >  1:3982202/1‑150 (MQ=255)
                                                  gcagGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTa                                                                                                     <  1:334027/150‑1 (MQ=255)
                                                   cagGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTAc                                                                                                    <  1:952187/150‑1 (MQ=255)
                                                    agGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTAcg                                                                                                   <  1:3651420/150‑1 (MQ=255)
                                                        tCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTAcgcgcg                                                                                               >  2:864924/1‑150 (MQ=255)
                                                          cGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAg                                                                                             >  1:4230512/1‑150 (MQ=255)
                                                          cGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAg                                                                                             >  1:1695447/1‑150 (MQ=255)
                                                           gATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGt                                                                                            <  1:813661/150‑1 (MQ=255)
                                                                ccGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCa                                                                                       <  2:1785911/150‑1 (MQ=255)
                                                                         cgcgGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAAc                                                                              <  2:2438483/150‑1 (MQ=255)
                                                                         cgcgGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAAc                                                                              <  1:3865867/150‑1 (MQ=255)
                                                                          gcgGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACa                                                                             <  2:1426492/150‑1 (MQ=255)
                                                                             ggCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCt                                                                          <  1:4513202/150‑1 (MQ=255)
                                                                                gATCCTAGACCGTCACATACACATATCACGCCGACTGGCGTAATCCGCTCACAAAACGGCTAACCATAATCAAGCGACTAACAGCCGCCCAATTACTGCCAAGATCCGAGAGCAACATTACGCGCGAGTGCGCACCTTCAAACACCggtt                                                                       >  2:2384582/1‑150 (MQ=255)
                                                                                  tCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCtgttgt                                                                     <  1:426818/150‑1 (MQ=255)
                                                                                  tCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCtgttgt                                                                     <  1:3043920/150‑1 (MQ=255)
                                                                                      cGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAg                                                                 <  1:1214812/150‑1 (MQ=255)
                                                                                      cGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAg                                                                 >  1:3661722/1‑150 (MQ=255)
                                                                                      cGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAg                                                                 >  2:3530003/1‑150 (MQ=255)
                                                                                       gACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGt                                                                <  2:812061/150‑1 (MQ=255)
                                                                                        aCGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGtt                                                               >  2:2047960/1‑150 (MQ=255)
                                                                                          ggTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGtttt                                                             <  1:1528739/150‑1 (MQ=255)
                                                                                          ggTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGtttt                                                             <  1:2848828/150‑1 (MQ=255)
                                                                                           gTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTc                                                            >  2:1532914/1‑150 (MQ=255)
                                                                                            tCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCa                                                           <  2:1472286/150‑1 (MQ=255)
                                                                                            tCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCa                                                           >  2:3112155/1‑150 (MQ=255)
                                                                                              acaTTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGc                                                         >  2:3945350/1‑150 (MQ=255)
                                                                                              acaTTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGc                                                         <  1:1110319/150‑1 (MQ=255)
                                                                                                   gtgCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAAc                                                    >  2:4037671/1‑150 (MQ=255)
                                                                                                     gCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACcg                                                  <  1:1783456/150‑1 (MQ=255)
                                                                                                       tGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACcgcg                                                >  1:1817469/1‑150 (MQ=255)
                                                                                                         tctcGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGAt                                              <  2:4348680/150‑1 (MQ=255)
                                                                                                         tctcGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGAt                                              <  2:3285557/150‑1 (MQ=255)
                                                                                                            cGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTg                                           >  2:1722780/1‑150 (MQ=255)
                                                                                                             gCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTgg                                          <  1:2720945/150‑1 (MQ=255)
                                                                                                               cGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGtt                                        <  2:2337867/150‑1 (MQ=255)
                                                                                                               cGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGtt                                        <  2:612933/150‑1 (MQ=255)
                                                                                                               cGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGtt                                        <  1:3299878/150‑1 (MQ=255)
                                                                                                                gACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTa                                       >  1:116613/1‑150 (MQ=255)
                                                                                                                 aCTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAg                                      >  1:4707085/1‑150 (MQ=255)
                                                                                                                   tGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCg                                    >  1:3013588/1‑150 (MQ=255)
                                                                                                                    ggCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGt                                   <  1:761230/150‑1 (MQ=255)
                                                                                                                     gCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTc                                  <  2:1980234/150‑1 (MQ=255)
                                                                                                                     gCGGAAGCCGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTc                                  <  1:130789/150‑1 (MQ=255)
                                                                                                                       ggAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCgg                                <  2:1622999/150‑1 (MQ=255)
                                                                                                                          aGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGgcgc                             <  1:4755187/150‑1 (MQ=255)
                                                                                                                             cGGGCACTATCCGGCTATCGATATAGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTa                          >  1:3166775/1‑150 (MQ=255)
                                                                                                                              gggCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTAt                         >  1:1752540/1‑150 (MQ=255)
                                                                                                                              gggCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTAt                         <  2:455230/150‑1 (MQ=255)
                                                                                                                                 cACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGcc                      <  1:3923742/150‑1 (MQ=255)
                                                                                                                                 cACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGcc                      <  1:4844468/150‑1 (MQ=255)
                                                                                                                                  aCTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCa                     <  2:4017450/150‑1 (MQ=255)
                                                                                                                                     aTCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAg                  <  1:4057627/150‑1 (MQ=255)
                                                                                                                                      tCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAgg                 <  2:1220633/150‑1 (MQ=255)
                                                                                                                                       ccGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGc                <  2:2977985/150‑1 (MQ=255)
                                                                                                                                       ccGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGGTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGc                <  2:4656093/150‑1 (MQ=255)
                                                                                                                                            tATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGa           >  2:498045/1‑150 (MQ=255)
                                                                                                                                                  tatTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGAt     <  2:2760120/150‑1 (MQ=255)
                                                                                                                                                   atTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATg    >  2:1949457/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCT  >  CP009273/2010989‑2011287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A