Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,105,545 C→A 28.5% W333L (TGG→TTG)  rfbB ← dTDP‑glucose 4,6 dehydratase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,105,5450CA28.5% 367.0 / 151.1 182W333L (TGG→TTG) rfbBdTDP‑glucose 4,6 dehydratase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (64/66);  new base A (25/27);  total (89/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 185 total aligned reads displayed.

CCAGAGCACGCTGTAGTTCCCAACCTACCTGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCT  >  CP009273/2105397‑2105694
                                                                                                                                                    |                                                                                                                                                     
   gagCACGCTGTAGTTCCCAACCTACCTGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTc                                                                                                                                                   >  1:3041927/1‑150 (MQ=255)
      cACGCTGTAGTTCCCAACCTACCTGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCAc                                                                                                                                                >  2:4628556/1‑150 (MQ=255)
       aCGCTGTAGTTCCCAACCTACCTGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCAcc                                                                                                                                               <  1:4500817/150‑1 (MQ=255)
       aCGCTGTAGTTCCCAACCTACCTGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCAcc                                                                                                                                               >  2:506909/1‑150 (MQ=255)
       aCGCTGTAGTTCCCAACCTACCTGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCAcc                                                                                                                                               <  1:4061009/150‑1 (MQ=255)
              aGTTCCCAACCTACCTGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTAc                                                                                                                                        >  2:3968732/1‑150 (MQ=255)
              aGTTCCCAACCTACCTGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTAc                                                                                                                                        >  1:3125458/1‑150 (MQ=255)
                   ccAACCTACCTGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATc                                                                                                                                   >  1:183729/1‑150 (MQ=255)
                           cctGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCtctc                                                                                                                           <  2:3989666/150‑1 (MQ=255)
                            ctGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCa                                                                                                                          <  1:1355591/150‑1 (MQ=255)
                            ctGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCa                                                                                                                          >  1:135965/1‑150 (MQ=255)
                               cccTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAAc                                                                                                                       <  1:2039488/150‑1 (MQ=255)
                                ccTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACg                                                                                                                      <  1:3018496/150‑1 (MQ=255)
                                ccTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACg                                                                                                                      <  1:561488/150‑1 (MQ=255)
                                 cTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGt                                                                                                                     >  2:3473828/1‑150 (MQ=255)
                                   gTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGttt                                                                                                                   >  1:2139157/1‑150 (MQ=255)
                                    ttttGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTc                                                                                                                  <  2:4098851/150‑1 (MQ=255)
                                      ttGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCt                                                                                                                >  2:3731232/1‑150 (MQ=255)
                                        gCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCtgt                                                                                                              <  1:1092104/150‑1 (MQ=255)
                                           aaaaaGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGt                                                                                                           >  1:4631706/1‑150 (MQ=255)
                                            aaaaGGAGGATATTCAGTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCACCCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGtt                                                                                                          <  2:2929605/150‑1 (MQ=255)
                                             aaaGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGttt                                                                                                         >  2:4738651/1‑150 (MQ=255)
                                             aaaGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAAACCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGttt                                                                                                         >  2:4453752/1‑150 (MQ=255)
                                              aaGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTc                                                                                                        <  2:4361990/150‑1 (MQ=255)
                                              aaGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTc                                                                                                        <  2:2684490/150‑1 (MQ=255)
                                              aaGGAGGATATTCATTACTGGCGGCCCCCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTc                                                                                                        >  2:782842/1‑150 (MQ=255)
                                                  aggaTATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATc                                                                                                    <  1:3244317/150‑1 (MQ=255)
                                                   ggaTATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATcc                                                                                                   >  1:1627282/1‑150 (MQ=255)
                                                   ggaTATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATcc                                                                                                   >  1:3674428/1‑150 (MQ=255)
                                                   ggaTATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATcc                                                                                                   >  1:2449899/1‑150 (MQ=255)
                                                    gaTATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATccc                                                                                                  >  1:2672006/1‑150 (MQ=255)
                                                    gaTATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATccc                                                                                                  >  1:2671753/1‑150 (MQ=255)
                                                      tatTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCaa                                                                                                >  1:1587857/1‑150 (MQ=255)
                                                      tatTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCaa                                                                                                >  1:1587690/1‑150 (MQ=255)
                                                       atTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAAt                                                                                               >  1:1849107/1‑150 (MQ=255)
                                                       atTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAAt                                                                                               <  2:1381495/150‑1 (MQ=255)
                                                        ttCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTATGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATg                                                                                              <  1:4071616/150‑1 (MQ=255)
                                                         tCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATgc                                                                                             >  1:255913/1‑150 (MQ=255)
                                                         tCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATgc                                                                                             <  2:1017857/150‑1 (MQ=255)
                                                         tCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATgc                                                                                             >  2:4663547/1‑150 (MQ=255)
                                                             tACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGa                                                                                         >  2:3138014/1‑150 (MQ=255)
                                                                  gcggcCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAAt                                                                                    <  2:765553/150‑1 (MQ=255)
                                                                       ccTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAAtcttct                                                                               >  1:1892602/1‑150 (MQ=255)
                                                                       ccTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAAtcttct                                                                               >  1:2236519/1‑150 (MQ=255)
                                                                          cATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAg                                                                            <  1:4320656/150‑1 (MQ=255)
                                                                          cATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAg                                                                            >  2:2443966/1‑150 (MQ=255)
                                                                          cATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAg                                                                            >  1:1713060/1‑150 (MQ=255)
                                                                            tAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCa                                                                          >  2:660199/1‑150 (MQ=255)
                                                                            aaGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCACTGCGCGACCAAACTTCTCAGCa                                                                          >  2:1188597/2‑150 (MQ=255)
                                                                               ttCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCa                                                                       <  2:4384330/150‑1 (MQ=255)
                                                                                    ttCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAatagc                                                                  <  1:388953/150‑1 (MQ=255)
                                                                                      cAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAatagcat                                                                <  1:3625490/150‑1 (MQ=255)
                                                                                       aaTCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAatagcata                                                               <  1:2318361/150‑1 (MQ=255)
                                                                                       aaTCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGGACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAatagcata                                                               <  2:3573773/150‑1 (MQ=255)
                                                                                         tCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAatagcatagc                                                             <  1:3714628/150‑1 (MQ=255)
                                                                                         tCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAatagcatagc                                                             <  1:3714584/150‑1 (MQ=255)
                                                                                             cGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAgcggcg                                                         >  2:3816817/1‑150 (MQ=255)
                                                                                                 tGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCg                                                     <  1:1018025/150‑1 (MQ=255)
                                                                                                 tGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCg                                                     <  1:1018044/150‑1 (MQ=255)
                                                                                                  gATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCCTCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCgt                                                    >  2:4599110/1‑150 (MQ=255)
                                                                                                    tAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCgtgt                                                  >  1:4043206/1‑150 (MQ=255)
                                                                                                      ggCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTcc                                                >  2:932944/1‑150 (MQ=255)
                                                                                                       gCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTccc                                               <  1:3805014/150‑1 (MQ=255)
                                                                                                       gCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTccc                                               >  1:3129321/1‑150 (MQ=255)
                                                                                                        caccacTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCg                                              >  2:234056/1‑150 (MQ=255)
                                                                                                          ccacTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGa                                            <  2:2626915/150‑1 (MQ=255)
                                                                                                          ccacTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGa                                            <  2:2626936/150‑1 (MQ=255)
                                                                                                           cacTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGAc                                           >  1:2822681/1‑150 (MQ=255)
                                                                                                           cacTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGAc                                           >  2:1744565/1‑150 (MQ=255)
                                                                                                              ttttCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGATTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCATCATCAATAGAATAGAGGCGATCGTGTCCCGGAAGAt                                        >  2:4629610/1‑150 (MQ=255)
                                                                                                               tttCACATTATCAACCCATTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATc                                       >  1:3271058/1‑150 (MQ=255)
                                                                                                                 tCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAg                                     >  1:4824751/1‑150 (MQ=255)
                                                                                                                   acaTTATCAACACATTTTGTATTGGACATGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCa                                   <  2:3076190/150‑1 (MQ=255)
                                                                                                                        aTCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATa                              >  2:3309202/1‑150 (MQ=255)
                                                                                                                         tCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATaa                             >  2:1788374/1‑150 (MQ=255)
                                                                                                                               cATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGAtt                       <  2:1061981/150‑1 (MQ=255)
                                                                                                                                aTTTTGTATTGGACAGTTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATAGTGTCCCGGACGATCAGCAACATAAGTGAttt                      <  2:707659/150‑1 (MQ=255)
                                                                                                                                aTTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGAttt                      <  1:3757085/150‑1 (MQ=255)
                                                                                                                                aTTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGAttt                      <  2:3215948/150‑1 (MQ=255)
                                                                                                                                aTTTTGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGAttt                      <  2:3797146/150‑1 (MQ=255)
                                                                                                                                 ttttGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTg                     <  2:3305957/150‑1 (MQ=255)
                                                                                                                                 ttttGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTg                     >  1:3095989/1‑150 (MQ=255)
                                                                                                                                 ttttGTATTGGACAGGTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTg                     <  2:309015/150‑1 (MQ=255)
                                                                                                                                   ttGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCt                   <  1:3922732/150‑1 (MQ=255)
                                                                                                                                     gTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCa                 >  2:2242355/1‑150 (MQ=255)
                                                                                                                                       aTTGGACCGGTACCATTCCACCGTGTTACGAATCCCGCTCTCAAACGGTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTGCTCCGCATCAATAGCATAGCGGCGATCGGGTCCCGGACGATCAGCTCCATAAGTGATTTGCTCACg               >  2:2906835/1‑150 (MQ=255)
                                                                                                                                          ggACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATa            >  2:778900/1‑150 (MQ=255)
                                                                                                                                           gACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATaa           <  2:3377791/150‑1 (MQ=255)
                                                                                                                                              aGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGAt        <  1:507609/150‑1 (MQ=255)
                                                                                                                                              aGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGAt        <  2:858102/150‑1 (MQ=255)
                                                                                                                                              aGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGAt        <  1:782842/150‑1 (MQ=255)
                                                                                                                                              aGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGAt        <  2:858131/150‑1 (MQ=255)
                                                                                                                                               ggTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGAtt       <  1:4606747/150‑1 (MQ=255)
                                                                                                                                               ggTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATaa           >  1:595976/1‑146 (MQ=255)
                                                                                                                                               ggTACAATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATaa           <  2:595976/146‑1 (MQ=255)
                                                                                                                                                 tACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTtc     <  2:415200/150‑1 (MQ=255)
                                                                                                                                                  aCCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTtct    <  1:3138014/150‑1 (MQ=255)
                                                                                                                                                  aCCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTtct    <  1:1180686/150‑1 (MQ=255)
                                                                                                                                                    cATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCCATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTctct  >  1:234262/1‑150 (MQ=255)
                                                                                                                                                    cATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTctct  >  2:2979082/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCAGAGCACGCTGTAGTTCCCAACCTACCTGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCT  >  CP009273/2105397‑2105694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A