Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,123,274 G→A 27.3% R362R (CGC→CGT)  wcaD ← putative colanic acid polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,123,2740GA27.3% 229.9 / 100.9 128R362R (CGC→CGT) wcaDputative colanic acid polymerase
Reads supporting (aligned to +/- strand):  ref base G (41/52);  new base A (18/17);  total (59/69)
Fisher's exact test for biased strand distribution p-value = 5.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 128 total aligned reads displayed.

AACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTC  >  CP009273/2123127‑2123422
                                                                                                                                                   |                                                                                                                                                    
aaCATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCg                                                                                                                                                    <  2:323017/150‑1 (MQ=255)
aaCATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCg                                                                                                                                                    <  1:602001/150‑1 (MQ=255)
aaCATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCg                                                                                                                                                    <  1:602002/150‑1 (MQ=255)
 aCATGTTCTTATTCGTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATAGTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCgg                                                                                                                                                   <  2:1353303/150‑1 (MQ=255)
     gTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTAt                                                                                                                                               <  2:4258577/150‑1 (MQ=255)
         ttattcttaCCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCAcc                                                                                                                                           <  2:4744682/150‑1 (MQ=255)
          tattcttaCCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCa                                                                                                                                          >  2:2147890/1‑150 (MQ=255)
          tattcttaCCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCa                                                                                                                                          >  2:4597950/1‑150 (MQ=255)
          tattcttaCCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCa                                                                                                                                          <  1:1516517/150‑1 (MQ=255)
              cttaCCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAg                                                                                                                                      <  1:2656595/150‑1 (MQ=255)
              cttaCCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAg                                                                                                                                      <  1:2656466/150‑1 (MQ=255)
               ttaCCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGc                                                                                                                                     >  2:4500071/1‑150 (MQ=255)
               ttaCCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGc                                                                                                                                     <  1:1820584/150‑1 (MQ=255)
               ttaCCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGc                                                                                                                                     <  1:1821118/150‑1 (MQ=255)
                  ccTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGtt                                                                                                                                  <  1:2179508/150‑1 (MQ=255)
                   cTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTg                                                                                                                                 <  2:3018266/150‑1 (MQ=255)
                       tAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGatca                                                                                                                             >  2:2218647/1‑150 (MQ=255)
                        aaTATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGatcat                                                                                                                            <  1:1853162/150‑1 (MQ=255)
                          tatTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGAtcatca                                                                                                                          <  2:2567948/150‑1 (MQ=255)
                             ttAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCAttt                                                                                                                       <  1:708035/150‑1 (MQ=255)
                             ttAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCAttt                                                                                                                       <  1:360183/150‑1 (MQ=255)
                              tAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCAtttt                                                                                                                      >  2:3664972/1‑150 (MQ=255)
                               aaCGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCAttttt                                                                                                                     <  1:2143086/150‑1 (MQ=255)
                                 cGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTAt                                                                                                                   <  1:2510054/150‑1 (MQ=255)
                                    ttttCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATTGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCAc                                                                                                                <  2:2852226/150‑1 (MQ=255)
                                       tCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACttt                                                                                                             <  1:2206097/150‑1 (MQ=255)
                                       tCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACttt                                                                                                             <  1:2204119/150‑1 (MQ=255)
                                         gCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTcc                                                                                                           >  1:370955/1‑150 (MQ=255)
                                           aaaaTAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTcccc                                                                                                         >  1:2443522/1‑150 (MQ=255)
                                           aaaaTAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTcccc                                                                                                         >  1:2445350/1‑150 (MQ=255)
                                           aaaaTAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTcccc                                                                                                         <  1:1481338/150‑1 (MQ=255)
                                           aaaaTAAACGGACAGACGATTAAAAATGCATAGTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTcccc                                                                                                         <  1:1481327/150‑1 (MQ=255)
                                            aaaTAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCa                                                                                                        <  1:761222/150‑1 (MQ=255)
                                            aaaTAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCa                                                                                                        <  2:4257217/150‑1 (MQ=255)
                                            aaaTAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCa                                                                                                        <  2:4256985/150‑1 (MQ=255)
                                            aaaTAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCa                                                                                                        <  2:4172048/150‑1 (MQ=255)
                                            aaaTAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCa                                                                                                        <  1:886900/150‑1 (MQ=255)
                                                  aCGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTAcc                                                                                                  <  1:4492933/150‑1 (MQ=255)
                                                   cGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACca                                                                                                 >  1:4504506/1‑150 (MQ=255)
                                                     gACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACcaca                                                                                               >  1:2352694/1‑150 (MQ=255)
                                                        aGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTg                                                                                            >  1:4836778/1‑150 (MQ=255)
                                                        aGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTg                                                                                            >  1:1379043/1‑150 (MQ=255)
                                                        aGACGATTAAAAATACATATTCCGGGCTACATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTg                                                                                            <  2:2802983/150‑1 (MQ=255)
                                                         gACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGa                                                                                           >  1:2481463/1‑150 (MQ=255)
                                                           cGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATa                                                                                         >  1:2324370/1‑150 (MQ=255)
                                                           cGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATa                                                                                         >  1:2324300/1‑150 (MQ=255)
                                                                     tGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACcgc                                                                               <  2:2825790/150‑1 (MQ=255)
                                                                       cATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGa                                                                             <  1:4008315/150‑1 (MQ=255)
                                                                       cATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGa                                                                             <  1:1564062/150‑1 (MQ=255)
                                                                        atatTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGaa                                                                            >  1:4221573/1‑150 (MQ=255)
                                                                           ttCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCa                                                                         <  2:4749926/150‑1 (MQ=255)
                                                                            tCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAg                                                                        <  1:2014207/150‑1 (MQ=255)
                                                                                 gCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGaaaa                                                                   <  1:4845613/150‑1 (MQ=255)
                                                                                  cTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACTAGGaaaaa                                                                  <  2:3817503/150‑1 (MQ=255)
                                                                                   tAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTAGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGACCCAGGAAaaat                                                                 >  1:135446/1‑150 (MQ=255)
                                                                                    aaaTATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAaaata                                                                <  1:3609415/150‑1 (MQ=255)
                                                                                    aaaTATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAaaata                                                                <  1:1150798/150‑1 (MQ=255)
                                                                                     aaTATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAaaataa                                                               <  2:4730920/150‑1 (MQ=255)
                                                                                          cGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAaataat                                                          <  1:921818/150‑1 (MQ=255)
                                                                                           gAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATc                                                         >  2:2076827/1‑150 (MQ=255)
                                                                                           gAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATc                                                         >  2:341647/1‑150 (MQ=255)
                                                                                             aCCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATcag                                                       >  1:4628355/1‑150 (MQ=255)
                                                                                               cGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATcagca                                                     >  1:4012791/1‑150 (MQ=255)
                                                                                                 gTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGa                                                   >  1:3972602/1‑150 (MQ=255)
                                                                                                     aaaaCAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACa                                               >  1:705920/1‑150 (MQ=255)
                                                                                                      aaaCAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACaa                                              <  1:1848478/150‑1 (MQ=255)
                                                                                                          aGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCa                                          >  1:2133532/1‑150 (MQ=255)
                                                                                                          aGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCa                                          >  1:2133148/1‑150 (MQ=255)
                                                                                                           gCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCAt                                         <  2:1196775/150‑1 (MQ=255)
                                                                                                             gATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTg                                       >  1:4079162/1‑150 (MQ=255)
                                                                                                             gATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTg                                       >  1:3913076/1‑150 (MQ=255)
                                                                                                               tACCGGAGTAAAAAGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTc                                     >  2:1820716/1‑150 (MQ=255)
                                                                                                                aCCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCt                                    >  1:1390803/1‑150 (MQ=255)
                                                                                                                aCCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCt                                    <  2:3440713/150‑1 (MQ=255)
                                                                                                                  cGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTAt                                  <  2:1115284/150‑1 (MQ=255)
                                                                                                                        aaaaaGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGtttt                            <  1:919090/150‑1 (MQ=255)
                                                                                                                          aaaGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTc                          >  1:1743036/1‑150 (MQ=255)
                                                                                                                          aaaGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTc                          >  1:1744069/1‑150 (MQ=255)
                                                                                                                           aaGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTcc                         >  2:1285274/1‑150 (MQ=255)
                                                                                                                           aaGATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTcc                         <  1:4500071/150‑1 (MQ=255)
                                                                                                                            aGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCg                        >  2:94707/1‑150 (MQ=255)
                                                                                                                            aGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCg                        <  1:3010802/150‑1 (MQ=255)
                                                                                                                            aGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCg                        >  2:1902879/1‑150 (MQ=255)
                                                                                                                             gATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGa                       >  2:4626176/1‑150 (MQ=255)
                                                                                                                             gATATAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGa                       <  1:485421/150‑1 (MQ=255)
                                                                                                                              atatAATTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGAc                      >  2:538836/1‑150 (MQ=255)
                                                                                                                                  aaTTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATcc                  <  1:2811942/150‑1 (MQ=255)
                                                                                                                                   aTTGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCg                 >  2:641885/1‑150 (MQ=255)
                                                                                                                                    ttGCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGc                >  1:3652284/1‑150 (MQ=255)
                                                                                                                                      gCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCAc              >  2:3405741/1‑150 (MQ=255)
                                                                                                                                      gCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCAc              >  2:959996/1‑150 (MQ=255)
                                                                                                                                      gCACGCGAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCACCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCAc              <  2:2799067/150‑1 (MQ=255)
                                                                                                                                         cgcgAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCAGTGTCTATGGTTTTTCCGACATCCGCACCGt           >  2:3320219/1‑150 (MQ=255)
                                                                                                                                           cgAAAATTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTa         >  2:3666076/1‑150 (MQ=255)
                                                                                                                                             aaaaTTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTaaa       <  1:164193/150‑1 (MQ=255)
                                                                                                                                              aaaTTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAat      >  2:3236706/1‑150 (MQ=255)
                                                                                                                                              aaaTTACGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAat      <  2:2352694/150‑1 (MQ=255)
                                                                                                                                                aTTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAatat    >  1:3667805/1‑150 (MQ=255)
                                                                                                                                                aTTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAatat    >  1:3667834/1‑150 (MQ=255)
                                                                                                                                                  tGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTc  >  2:2349156/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTC  >  CP009273/2123127‑2123422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A