Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,194,695 T→A 68.9% L313Q (CTA→CAA)  yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,194,6950TA68.9% 427.0 / 278.4 267L313Q (CTA→CAA) yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (36/47);  new base A (84/100);  total (120/147)
Fisher's exact test for biased strand distribution p-value = 7.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 268 total aligned reads displayed.

TGAAAACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGACAA  >  CP009273/2194546‑2194842
                                                                                                                                                     |                                                                                                                                                   
tGAAAACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCt                                                                                                                                                     >  1:255179/1‑150 (MQ=255)
     aCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtgg                                                                                                                                                <  2:122368/150‑1 (MQ=255)
     aCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtgg                                                                                                                                                <  2:121395/150‑1 (MQ=255)
      ccACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtggt                                                                                                                                               >  1:2827312/1‑150 (MQ=255)
         cTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGc                                                                                                                                            <  1:3164128/150‑1 (MQ=255)
         cTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGc                                                                                                                                            >  2:4186995/1‑150 (MQ=255)
           tCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAACGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTg                                                                                                                                          <  2:3683351/150‑1 (MQ=255)
           tCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTg                                                                                                                                          >  1:2601122/1‑150 (MQ=255)
             ggCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGAt                                                                                                                                        >  2:714470/1‑150 (MQ=255)
             ggCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGAt                                                                                                                                        >  2:4165185/1‑150 (MQ=255)
             ggCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGAt                                                                                                                                        >  2:1113914/1‑150 (MQ=255)
                tACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATaaa                                                                                                                                     >  2:2931460/1‑150 (MQ=255)
                     ggCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTCCTGGTGGCTGATAAaatcaa                                                                                                                                >  2:3248026/1‑150 (MQ=255)
                       ccTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAaaacaaac                                                                                                                              >  1:2098481/1‑150 (MQ=255)
                          gcgcCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCa                                                                                                                           <  1:4789057/150‑1 (MQ=255)
                              cgccCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCt                                                                                                                       >  2:1942310/1‑150 (MQ=255)
                              cgccCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCt                                                                                                                       <  1:4322927/150‑1 (MQ=255)
                                cccTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGc                                                                                                                     >  1:3468842/1‑150 (MQ=255)
                                 ccTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCa                                                                                                                    >  1:1218215/1‑150 (MQ=255)
                                  cTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCaa                                                                                                                   >  2:2002138/1‑150 (MQ=255)
                                   ttttGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAAc                                                                                                                  <  2:3769088/150‑1 (MQ=255)
                                   ttttGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAAc                                                                                                                  <  2:500224/150‑1 (MQ=255)
                                   ttttGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAAc                                                                                                                  >  2:1706548/1‑150 (MQ=255)
                                   ttttGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAAc                                                                                                                  <  2:4561062/150‑1 (MQ=255)
                                    tttGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACg                                                                                                                 >  1:2488304/1‑150 (MQ=255)
                                      tGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACgtg                                                                                                               <  1:2161554/150‑1 (MQ=255)
                                      tGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACgtg                                                                                                               <  2:2084593/150‑1 (MQ=255)
                                          cgAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGc                                                                                                           >  1:2049425/1‑150 (MQ=255)
                                              cAAGGACTGGCAGCCAGTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTaaa                                                                                                       <  1:4185753/150‑1 (MQ=255)
                                               aaGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAAt                                                                                                      >  2:3761491/1‑150 (MQ=255)
                                               aaGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAAt                                                                                                      <  1:1009011/150‑1 (MQ=255)
                                                aGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCGTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATa                                                                                                     <  2:2071750/150‑1 (MQ=255)
                                                   aCTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCa                                                                                                  <  1:895552/150‑1 (MQ=255)
                                                   aCTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCa                                                                                                  >  1:1271215/1‑150 (MQ=255)
                                                    cTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCAt                                                                                                 >  2:851734/1‑150 (MQ=255)
                                                     tGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATa                                                                                                >  2:4731131/1‑150 (MQ=255)
                                                      ggCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaa                                                                                               <  2:670164/150‑1 (MQ=255)
                                                        cAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaaaa                                                                                             <  2:1293910/150‑1 (MQ=255)
                                                           ccATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACt                                                                                          <  2:313397/150‑1 (MQ=255)
                                                            cATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACtt                                                                                         <  2:2849707/150‑1 (MQ=255)
                                                            cATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACtt                                                                                         <  1:4553304/150‑1 (MQ=255)
                                                            cATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACtt                                                                                         >  1:4402872/1‑150 (MQ=255)
                                                            cATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACtt                                                                                         <  1:2997420/150‑1 (MQ=255)
                                                            cATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGAGTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACtt                                                                                         <  2:417519/150‑1 (MQ=255)
                                                               tCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTccc                                                                                      <  2:4502970/150‑1 (MQ=255)
                                                                   cgcCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCag                                                                                  >  2:4392473/1‑149 (MQ=255)
                                                                   cgcCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCAt                                                                                  >  2:26886/1‑150 (MQ=255)
                                                                    gcCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATg                                                                                 <  2:2668059/150‑1 (MQ=255)
                                                                      cTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCg                                                                               <  2:4008354/150‑1 (MQ=255)
                                                                          cgATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGc                                                                           >  2:1568760/1‑150 (MQ=255)
                                                                            aTGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTc                                                                         >  1:1827933/1‑150 (MQ=255)
                                                                            aTGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTc                                                                         >  2:3882074/1‑150 (MQ=255)
                                                                                aTGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTcgccg                                                                     <  1:3959380/150‑1 (MQ=255)
                                                                                aTGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTcgccg                                                                     <  1:73952/150‑1 (MQ=255)
                                                                                  gCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAAATGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCa                                                                   <  2:2602066/150‑1 (MQ=255)
                                                                                    ccATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACt                                                                 <  2:1953049/150‑1 (MQ=255)
                                                                                    ccATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACt                                                                 <  2:2235354/150‑1 (MQ=255)
                                                                                     cATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTg                                                                >  2:60885/1‑150 (MQ=255)
                                                                                      aTAAAGAAGAGTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGTTGATAAAAACAAACCCCGCCTGCAACGTGTAGCTAAATACCATAAAAACGTCCCCCCTGCGACGCTCGCCGCACTgg                                                               <  1:4530059/150‑1 (MQ=255)
                                                                                            aagaTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAAc                                                         >  2:2742546/1‑150 (MQ=255)
                                                                                            aagaTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAAc                                                         <  2:2966142/150‑1 (MQ=255)
                                                                                            aagaTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAAc                                                         <  2:534023/150‑1 (MQ=255)
                                                                                             agaTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAAct                                                        >  2:1864767/1‑150 (MQ=255)
                                                                                             agaTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAAct                                                        <  1:3772490/150‑1 (MQ=255)
                                                                                               aTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAActgc                                                      >  2:950973/1‑150 (MQ=255)
                                                                                               aTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAActgc                                                      >  2:3951856/1‑150 (MQ=255)
                                                                                               aTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAActgc                                                      >  1:2886861/1‑150 (MQ=255)
                                                                                                ttGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAActgct                                                     <  1:1039476/150‑1 (MQ=255)
                                                                                                ttGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAActgct                                                     <  2:3621370/150‑1 (MQ=255)
                                                                                                   cGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGc                                                  <  1:265096/150‑1 (MQ=255)
                                                                                                   cGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGc                                                  <  1:264078/150‑1 (MQ=255)
                                                                                                   cGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGc                                                  <  1:82811/150‑1 (MQ=255)
                                                                                                         tCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAAc                                            >  2:389655/1‑150 (MQ=255)
                                                                                                         tCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAAc                                            >  2:2818437/1‑150 (MQ=255)
                                                                                                          ctgctgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACa                                           <  1:4854951/150‑1 (MQ=255)
                                                                                                            gctgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGa                                         <  1:4373798/150‑1 (MQ=255)
                                                                                                             ctgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGaa                                        <  1:1719701/150‑1 (MQ=255)
                                                                                                              tgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCATGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAac                                       <  1:181528/150‑1 (MQ=255)
                                                                                                                gTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAacca                                     >  1:1396723/1‑150 (MQ=255)
                                                                                                                gTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACCGGCAGAACTGCTGGCGTTAACAGAacca                                     <  1:1993285/150‑1 (MQ=255)
                                                                                                                 tACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAaccac                                    >  1:3321157/1‑150 (MQ=255)
                                                                                                                    aaaTTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAg                                 >  2:4838316/1‑150 (MQ=255)
                                                                                                                     aaTTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgc                                >  2:3445760/1‑150 (MQ=255)
                                                                                                                         aaCCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcccgc                            >  1:4705422/1‑150 (MQ=255)
                                                                                                                         aaCCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcccgc                            >  1:1160723/1‑150 (MQ=255)
                                                                                                                           ccATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcccgccc                          <  2:76739/150‑1 (MQ=255)
                                                                                                                           ccATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcccgccc                          >  1:3606811/1‑150 (MQ=255)
                                                                                                                            cATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAACACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCt                         <  2:1399634/150‑1 (MQ=255)
                                                                                                                              tCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTgg                       >  2:2136951/1‑150 (MQ=255)
                                                                                                                                cGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGtt                     <  2:4019819/150‑1 (MQ=255)
                                                                                                                                cGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGtt                     <  1:3890306/150‑1 (MQ=255)
                                                                                                                                cGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGtt                     <  1:1609744/150‑1 (MQ=255)
                                                                                                                                   aaGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATc                  <  2:2092515/150‑1 (MQ=255)
                                                                                                                                   aaGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATc                  <  2:2092599/150‑1 (MQ=255)
                                                                                                                                   aaGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCCGCCCGCCCTGGTTATc                  >  1:427643/1‑150 (MQ=255)
                                                                                                                                    aGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATcc                 <  2:4458292/150‑1 (MQ=255)
                                                                                                                                       cATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAat              <  1:2822505/150‑1 (MQ=255)
                                                                                                                                         tCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAatca            <  2:4038909/150‑1 (MQ=255)
                                                                                                                                                  tGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGACa   >  1:4461960/1‑150 (MQ=255)
                                                                                                                                                   gCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGACaa  <  1:1568760/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGAAAACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGACAA  >  CP009273/2194546‑2194842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGAAAACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGACAA  >  CP009273/2194546‑2194842
                                                                                                                                                     |                                                                                                                                                   
TGAAAACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCT                                                                                                                                                     >  SRR19973081.257248/1‑150 (MQ=60)
  AAAACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAAC                                                                                                                                                   >  SRR19973081.4747972/1‑150 (MQ=60)
    AACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTG                                                                                                                                                 >  SRR19973081.4530162/1‑150 (MQ=60)
     ACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAATATTGCGGCAGTCTGCTCGTCCAAATTAACCATCCGCAAGACATCCGCACCTAGCAACTGG                                                                                                                                                >  SRR19973081.1328322/1‑150 (MQ=60)
      CCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGT                                                                                                                                               >  SRR19973081.2844460/1‑150 (MQ=60)
      CCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGT                                                                                                                                               >  SRR19973081.2844459/1‑150 (MQ=60)
      CCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGT                                                                                                                                               >  SRR19973081.2844232/1‑150 (MQ=60)
       CACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTG                                                                                                                                              <  SRR19973081.4417126/150‑1 (MQ=60)
         CTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGC                                                                                                                                            <  SRR19973081.3183674/150‑1 (MQ=60)
         CTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGC                                                                                                                                            <  SRR19973081.3183397/150‑1 (MQ=60)
           TCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTG                                                                                                                                          >  SRR19973081.2618071/1‑150 (MQ=60)
           TCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTG                                                                                                                                          >  SRR19973081.2618044/1‑150 (MQ=60)
           TCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTG                                                                                                                                          >  SRR19973081.2617328/1‑150 (MQ=60)
              GCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATA                                                                                                                                       <  SRR19973081.858040/150‑1 (MQ=60)
               CTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAA                                                                                                                                      <  SRR19973081.4777562/150‑1 (MQ=60)
                      GCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAA                                                                                                                               >  SRR19973081.4217235/1‑150 (MQ=60)
                       CCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAAC                                                                                                                              >  SRR19973081.2111841/1‑150 (MQ=60)
                       CCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAAC                                                                                                                              >  SRR19973081.2111376/1‑150 (MQ=60)
                          GCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCA                                                                                                                           <  SRR19973081.4817938/150‑1 (MQ=60)
                              CGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCT                                                                                                                       >  SRR19973081.3123850/1‑150 (MQ=60)
                              CGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCT                                                                                                                       <  SRR19973081.4349208/150‑1 (MQ=60)
                                CCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGC                                                                                                                     >  SRR19973081.3490150/1‑150 (MQ=60)
                                CCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGC                                                                                                                     >  SRR19973081.1574826/1‑150 (MQ=60)
                                 CCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCA                                                                                                                    >  SRR19973081.1226136/1‑150 (MQ=60)
                                   TTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAAC                                                                                                                  >  SRR19973081.4188943/1‑150 (MQ=60)
                                    TTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACG                                                                                                                 >  SRR19973081.2503785/1‑150 (MQ=60)
                                      TGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTG                                                                                                               <  SRR19973081.3782598/150‑1 (MQ=60)
                                      TGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTG                                                                                                               <  SRR19973081.2175322/150‑1 (MQ=60)
                                       GCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGTCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCTCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGT                                                                                                              <  SRR19973081.720076/150‑1 (MQ=60)
                                       GCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGT                                                                                                              <  SRR19973081.1826131/150‑1 (MQ=60)
                                       GCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGT                                                                                                              <  SRR19973081.1826144/150‑1 (MQ=60)
                                          CGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGC                                                                                                           >  SRR19973081.2062334/1‑150 (MQ=60)
                                           GAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCT                                                                                                          <  SRR19973081.972018/150‑1 (MQ=60)
                                            AACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTA                                                                                                         <  SRR19973081.1034418/150‑1 (MQ=60)
                                              CAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAA                                                                                                       >  SRR19973081.3815413/1‑150 (MQ=60)
                                              CAAGGACTGGCAGCCAGTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAA                                                                                                       <  SRR19973081.4211343/150‑1 (MQ=60)
                                               AAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAAT                                                                                                      <  SRR19973081.1015854/150‑1 (MQ=60)
                                               AAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAAT                                                                                                      <  SRR19973081.446783/150‑1 (MQ=60)
                                                AGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATA                                                                                                     <  SRR19973081.2169081/150‑1 (MQ=60)
                                                AGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATA                                                                                                     <  SRR19973081.2167816/150‑1 (MQ=60)
                                                 GGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATAC                                                                                                    >  SRR19973081.4386865/1‑150 (MQ=60)
                                                  GACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACC                                                                                                   <  SRR19973081.3126908/150‑1 (MQ=60)
                                                   ACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCA                                                                                                  <  SRR19973081.901551/150‑1 (MQ=60)
                                                   ACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCA                                                                                                  >  SRR19973081.1279629/1‑150 (MQ=60)
                                                   ACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCA                                                                                                  >  SRR19973081.3754182/1‑150 (MQ=60)
                                                          GCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAAC                                                                                           <  SRR19973081.3444197/150‑1 (MQ=60)
                                                            CATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTT                                                                                         >  SRR19973081.4429400/1‑150 (MQ=60)
                                                            CATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTT                                                                                         <  SRR19973081.4580908/150‑1 (MQ=60)
                                                            CATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTT                                                                                         <  SRR19973081.3015896/150‑1 (MQ=60)
                                                                          CGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGC                                                                           <  SRR19973081.2675784/150‑1 (MQ=60)
                                                                           GATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCT                                                                          <  SRR19973081.4558048/150‑1 (MQ=60)
                                                                            ATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTC                                                                         >  SRR19973081.1840989/1‑150 (MQ=60)
                                                                            ATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTC                                                                         >  SRR19973081.1839834/1‑150 (MQ=60)
                                                                            ATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTC                                                                         >  SRR19973081.271198/1‑150 (MQ=60)
                                                                                ATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCG                                                                     <  SRR19973081.3983596/150‑1 (MQ=60)
                                                                                ATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCG                                                                     <  SRR19973081.74782/150‑1 (MQ=60)
                                                                                ATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCG                                                                     <  SRR19973081.74813/150‑1 (MQ=60)
                                                                                 TGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGC                                                                    >  SRR19973081.3919672/1‑150 (MQ=60)
                                                                                  GCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCA                                                                   >  SRR19973081.1401422/1‑150 (MQ=60)
                                                                                     CATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTG                                                                >  SRR19973081.2378258/1‑150 (MQ=60)
                                                                                     CATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTG                                                                >  SRR19973081.2378255/1‑150 (MQ=60)
                                                                                      ATAAAGAAGAGTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGTTGATAAAAACAAACCCCGCCTGCAACGTGTAGCTAAATACCATAAAAACGTCCCCCCTGCGACGCTCGCCGCACTGG                                                               <  SRR19973081.4557492/150‑1 (MQ=60)
                                                                                        AAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCA                                                             <  SRR19973081.4473859/150‑1 (MQ=60)
                                                                                          AGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGA                                                           >  SRR19973081.2586000/1‑150 (MQ=60)
                                                                                          AGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGA                                                           >  SRR19973081.2585985/1‑150 (MQ=60)
                                                                                             AGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACT                                                        <  SRR19973081.3795639/150‑1 (MQ=60)
                                                                                               ATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGC                                                      >  SRR19973081.2904597/1‑150 (MQ=60)
                                                                                               ATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGC                                                      >  SRR19973081.2904559/1‑150 (MQ=60)
                                                                                                TTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCT                                                     <  SRR19973081.1046472/150‑1 (MQ=60)
                                                                                                   CGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGC                                                  <  SRR19973081.83713/150‑1 (MQ=60)
                                                                                                   CGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGC                                                  <  SRR19973081.83548/150‑1 (MQ=60)
                                                                                                   CGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGC                                                  <  SRR19973081.266190/150‑1 (MQ=60)
                                                                                                   CGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGC                                                  <  SRR19973081.266229/150‑1 (MQ=60)
                                                                                                   CGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGC                                                  <  SRR19973081.267211/150‑1 (MQ=60)
                                                                                                    GGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCG                                                 >  SRR19973081.2029359/1‑150 (MQ=60)
                                                                                                    GGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCG                                                 >  SRR19973081.2031213/1‑150 (MQ=60)
                                                                                                          CTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACA                                           <  SRR19973081.4884090/150‑1 (MQ=60)
                                                                                                          CTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACA                                           <  SRR19973081.4884087/150‑1 (MQ=60)
                                                                                                          CTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACA                                           <  SRR19973081.4883698/150‑1 (MQ=60)
                                                                                                            GCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGA                                         <  SRR19973081.4400246/150‑1 (MQ=60)
                                                                                                             CTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAA                                        <  SRR19973081.1730978/150‑1 (MQ=60)
                                                                                                              TGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAAC                                       <  SRR19973081.4326546/150‑1 (MQ=60)
                                                                                                              TGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAAC                                       >  SRR19973081.1252002/1‑150 (MQ=60)
                                                                                                              TGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAAC                                       <  SRR19973081.183307/150‑1 (MQ=60)
                                                                                                              TGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCATGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAAC                                       <  SRR19973081.183145/150‑1 (MQ=60)
                                                                                                                GTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCA                                     >  SRR19973081.3941458/1‑150 (MQ=60)
                                                                                                                GTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCA                                     >  SRR19973081.1406087/1‑150 (MQ=60)
                                                                                                                GTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCA                                     >  SRR19973081.1406819/1‑150 (MQ=60)
                                                                                                                GTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACCGGCAGAACTGCTGGCGTTAACAGAACCA                                     <  SRR19973081.2005750/150‑1 (MQ=60)
                                                                                                                 TACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCAC                                    >  SRR19973081.3341478/1‑150 (MQ=60)
                                                                                                                         AACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGC                            >  SRR19973081.1168199/1‑150 (MQ=60)
                                                                                                                         AACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGC                            >  SRR19973081.4733941/1‑150 (MQ=60)
                                                                                                                         AACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGC                            >  SRR19973081.4733515/1‑150 (MQ=60)
                                                                                                                           CCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCC                          >  SRR19973081.1157594/1‑150 (MQ=60)
                                                                                                                           CCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCC                          >  SRR19973081.3628655/1‑150 (MQ=60)
                                                                                                                           CCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCC                          >  SRR19973081.2993236/1‑150 (MQ=60)
                                                                                                                            CATCCGCAAGTCATCCGCACCTCGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCT                         <  SRR19973081.2950162/150‑1 (MQ=60)
                                                                                                                                CGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTT                     <  SRR19973081.3914210/150‑1 (MQ=60)
                                                                                                                                CGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTT                     <  SRR19973081.1620536/150‑1 (MQ=60)
                                                                                                                                CGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTT                     <  SRR19973081.1620102/150‑1 (MQ=60)
                                                                                                                                CGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTT                     <  SRR19973081.1620062/150‑1 (MQ=60)
                                                                                                                                CGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTT                     <  SRR19973081.1620561/150‑1 (MQ=60)
                                                                                                                                   AAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCCGCCCGCCCTGGTTATC                  >  SRR19973081.430490/1‑150 (MQ=60)
                                                                                                                                   AAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATC                  >  SRR19973081.3503059/1‑150 (MQ=60)
                                                                                                                                   AAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATC                  >  SRR19973081.4130019/1‑150 (MQ=60)
                                                                                                                                   AAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATC                  >  SRR19973081.3503223/1‑150 (MQ=60)
                                                                                                                                       CATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAAT              <  SRR19973081.2839640/150‑1 (MQ=60)
                                                                                                                                              ACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAA       >  SRR19973081.4309497/1‑150 (MQ=60)
                                                                                                                                              ACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAA       >  SRR19973081.4365237/1‑150 (MQ=60)
                                                                                                                                              ACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAA       >  SRR19973081.1896287/1‑150 (MQ=60)
                                                                                                                                              ACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAA       <  SRR19973081.1195232/150‑1 (MQ=60)
                                                                                                                                              ACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAA       <  SRR19973081.1195826/150‑1 (MQ=60)
                                                                                                                                                  TGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGACA   >  SRR19973081.3309956/1‑150 (MQ=60)
                                                                                                                                                  TGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGACA   <  SRR19973081.4595836/150‑1 (MQ=60)
                                                                                                                                                  TGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGACA   >  SRR19973081.4489565/1‑150 (MQ=60)
                                                                                                                                                  TGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGACA   >  SRR19973081.253987/1‑150 (MQ=60)
                                                                                                                                                  TGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGACA   >  SRR19973081.4488726/1‑150 (MQ=60)
                                                                                                                                                   GCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGACAA  <  SRR19973081.1076648/150‑1 (MQ=60)
                                                                                                                                                   GCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGACAA  <  SRR19973081.1578900/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
TGAAAACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGACAA  >  CP009273/2194546‑2194842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑