Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,429,812 A→T 20.7% V106E (GTA→GAA)  usg ← putative semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,429,8120AT20.7% 801.4 / 203.1 323V106E (GTA→GAA) usgputative semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (132/124);  new base T (38/29);  total (170/153)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 326 total aligned reads displayed.

TAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGC  >  CP009273/2429663‑2429961
                                                                                                                                                     |                                                                                                                                                     
 aaCGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTAc                                                                                                                                                      >  1:1568339/1‑150 (MQ=255)
 aaCGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTAc                                                                                                                                                      <  1:4361881/150‑1 (MQ=255)
     cTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCg                                                                                                                                                  >  2:4055575/1‑150 (MQ=255)
       gATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCggg                                                                                                                                                >  1:1489262/1‑150 (MQ=255)
        aTACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGt                                                                                                                                               >  2:3496470/1‑150 (MQ=255)
        aTACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGt                                                                                                                                               >  2:3146970/1‑150 (MQ=255)
         tACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGtt                                                                                                                                              <  2:1656692/150‑1 (MQ=255)
          aCGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTc                                                                                                                                             <  2:4422162/150‑1 (MQ=255)
           cGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCg                                                                                                                                            >  1:4410728/1‑150 (MQ=255)
                tAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGc                                                                                                                                       <  2:4520286/150‑1 (MQ=255)
                       cccTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTTCGTCGGGTTCGAGAGCAAACAAt                                                                                                                                >  2:4805160/1‑150 (MQ=255)
                        ccTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATc                                                                                                                               <  2:2198202/150‑1 (MQ=255)
                        ccTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATc                                                                                                                               >  1:1005129/1‑150 (MQ=255)
                         cTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATcc                                                                                                                              >  1:3876299/1‑150 (MQ=255)
                         cTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATcc                                                                                                                              <  1:1201471/150‑1 (MQ=255)
                          tGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTTCGTCGGGTTCGAGAGCAAACAATCCa                                                                                                                             >  2:807714/1‑150 (MQ=255)
                          tGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCa                                                                                                                             >  1:2819477/1‑150 (MQ=255)
                           gATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCAc                                                                                                                            <  2:641907/150‑1 (MQ=255)
                            aTCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCAct                                                                                                                           <  2:3804648/150‑1 (MQ=255)
                            aTCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCAct                                                                                                                           >  1:857369/1‑150 (MQ=255)
                             tCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCActg                                                                                                                          >  1:3792066/1‑150 (MQ=255)
                                   aaCGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCg                                                                                                                    <  1:4655111/150‑1 (MQ=255)
                                   aaCGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCg                                                                                                                    >  2:1367713/1‑150 (MQ=255)
                                   aaCGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCg                                                                                                                    <  1:658598/150‑1 (MQ=255)
                                     cGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGAt                                                                                                                  >  1:2628817/1‑150 (MQ=255)
                                       gTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCa                                                                                                                >  2:1320839/1‑150 (MQ=255)
                                       gTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCa                                                                                                                >  1:4733235/1‑150 (MQ=255)
                                       gTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCa                                                                                                                <  1:1599755/150‑1 (MQ=255)
                                        tttCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCAc                                                                                                               <  2:4742521/150‑1 (MQ=255)
                                        tttCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCAc                                                                                                               <  2:4465304/150‑1 (MQ=255)
                                         ttCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCAcc                                                                                                              >  1:3075227/1‑150 (MQ=255)
                                          tCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCa                                                                                                             <  2:1877797/150‑1 (MQ=255)
                                                  gccAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCt                                                                                                     >  2:2384571/1‑150 (MQ=255)
                                                   ccAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTg                                                                                                    >  1:1998133/1‑150 (MQ=255)
                                                    cagcagCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGa                                                                                                   >  1:4082750/1‑150 (MQ=255)
                                                    cagcagCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGa                                                                                                   <  1:4515916/150‑1 (MQ=255)
                                                     agcagcTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAg                                                                                                  >  1:4422129/1‑150 (MQ=255)
                                                     agcagcTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAg                                                                                                  >  2:4678063/1‑150 (MQ=255)
                                                     agcagcTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAg                                                                                                  >  1:2107615/1‑150 (MQ=255)
                                                       cagcTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGtt                                                                                                <  1:3545309/150‑1 (MQ=255)
                                                           tggctggTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTc                                                                                            >  1:602428/1‑150 (MQ=255)
                                                            ggctggTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCg                                                                                           >  1:2460753/1‑150 (MQ=255)
                                                                   cAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCttcttc                                                                                    >  1:4816090/1‑150 (MQ=255)
                                                                     gACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa                                                                                  <  2:3106371/150‑1 (MQ=255)
                                                                             ggTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCa                                                                          >  1:4470547/1‑150 (MQ=255)
                                                                              gTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAg                                                                         <  1:1080941/150‑1 (MQ=255)
                                                                                aCGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCg                                                                       >  1:1283264/1‑150 (MQ=255)
                                                                                aCGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCg                                                                       <  2:678056/150‑1 (MQ=255)
                                                                                 cGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCgg                                                                      >  2:2543972/1‑150 (MQ=255)
                                                                                   gCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTa                                                                    <  1:735483/150‑1 (MQ=255)
                                                                                    cGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAg                                                                   >  1:3909152/1‑150 (MQ=255)
                                                                                    cGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAg                                                                   >  2:1338773/1‑150 (MQ=255)
                                                                                    cGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAg                                                                   >  1:1448459/1‑150 (MQ=255)
                                                                                     gATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGc                                                                  >  2:3919109/1‑150 (MQ=255)
                                                                                       tGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGctt                                                                >  1:1812564/1‑150 (MQ=255)
                                                                                           aTTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCttt                                                            <  1:2351136/150‑1 (MQ=255)
                                                                                           aTTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCttt                                                            <  2:146278/150‑1 (MQ=255)
                                                                                           aTTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACGTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCttt                                                            <  2:2469573/150‑1 (MQ=255)
                                                                                            ttccggttccggTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTg                                                           >  1:4264054/1‑150 (MQ=255)
                                                                                            ttccggttccggTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTg                                                           >  1:361997/1‑150 (MQ=255)
                                                                                            ttccggttccggTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTg                                                           <  1:3740752/150‑1 (MQ=255)
                                                                                             tccggttccggtAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGc                                                          <  1:3567456/150‑1 (MQ=255)
                                                                                              ccggttccggtAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGcc                                                         >  2:172547/1‑150 (MQ=255)
                                                                                              ccggttccggtAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGcc                                                         >  1:1096745/1‑150 (MQ=255)
                                                                                                ggttccggtAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTg                                                       >  2:64254/1‑150 (MQ=255)
                                                                                                     cggtAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACa                                                  <  2:104157/150‑1 (MQ=255)
                                                                                                     cggtAATCTGACAGTACAAACGGGTTTACTTCAGGCACCACCAGCGGTACGTCGGGTTCGAGATCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGAct                                                  <  2:111831/150‑2 (MQ=255)
                                                                                                        tAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTATTTGCCTGCGACaaaa                                               >  1:3665405/1‑150 (MQ=255)
                                                                                                        tAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACaaaa                                               <  1:4682002/150‑1 (MQ=255)
                                                                                                        tAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACaaaa                                               >  1:4774648/1‑150 (MQ=255)
                                                                                                         aaTCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACaaaaa                                              >  2:2252831/1‑150 (MQ=255)
                                                                                                         aaTCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACaaaaa                                              >  1:951323/1‑150 (MQ=255)
                                                                                                              gTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGcc                                         >  1:2038674/1‑150 (MQ=255)
                                                                                                                cAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAg                                       <  2:3432323/150‑1 (MQ=255)
                                                                                                                     cAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTgcg                                  <  2:1933604/150‑1 (MQ=255)
                                                                                                                        aCGGGTTTACTTCCGGCACCACCAGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCt                               <  2:477404/150‑1 (MQ=255)
                                                                                                                          gggTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGc                             <  2:4375475/150‑1 (MQ=255)
                                                                                                                              ttACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTcc                         <  2:602428/150‑1 (MQ=255)
                                                                                                                              ttACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTcc                         >  2:3326485/1‑150 (MQ=255)
                                                                                                                               tACTTCCTGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCa                        <  2:4003381/150‑1 (MQ=255)
                                                                                                                               tACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCa                        <  2:2107615/150‑1 (MQ=255)
                                                                                                                                aCTTCCGGCACCACCAGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAg                       >  2:789746/1‑150 (MQ=255)
                                                                                                                                aCTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAg                       >  2:353454/1‑150 (MQ=255)
                                                                                                                                   tCCGGCACCACCAGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCg                    >  1:2297149/1‑150 (MQ=255)
                                                                                                                                     cGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGaa                  >  1:2511058/1‑150 (MQ=255)
                                                                                                                                     cGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGaa                  >  2:881825/1‑150 (MQ=255)
                                                                                                                                      ggCACCACCAGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAAt                 <  2:3904927/150‑1 (MQ=255)
                                                                                                                                      ggCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAAt                 >  2:4273222/1‑150 (MQ=255)
                                                                                                                                       gCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAAtt                <  2:1558273/150‑1 (MQ=255)
                                                                                                                                       gCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAAtt                <  1:2992601/150‑1 (MQ=255)
                                                                                                                                          ccaccaGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAg             <  2:123919/150‑1 (MQ=255)
                                                                                                                                          ccaccaGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAg             <  1:3219950/150‑1 (MQ=255)
                                                                                                                                           caccaGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGc            <  1:134544/150‑1 (MQ=255)
                                                                                                                                            accaGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCg           <  2:4131472/150‑1 (MQ=255)
                                                                                                                                             ccaGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCgg          <  2:2930561/150‑1 (MQ=255)
                                                                                                                                               aGCGGTTCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCa        >  1:4018459/1‑150 (MQ=255)
                                                                                                                                               aGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCa        >  1:863415/1‑150 (MQ=255)
                                                                                                                                                   gTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCt    <  2:2819477/150‑1 (MQ=255)
                                                                                                                                                    tACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCCGCGTCCAGTCGAATTCAGCGGCATCCTg   >  2:2150330/1‑150 (MQ=255)
                                                                                                                                                     aCGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGc  <  2:3731918/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGC  >  CP009273/2429663‑2429961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A