Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,495,786 C→T 25.0% D727N (GAT→AAT)  fryA ← putative PTS enzyme, Hpr component/enzyme I component/enzyme IIA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,495,7860CT25.0% 538.1 / 195.6 273D727N (GAT→AAT) fryAputative PTS enzyme, Hpr component/enzyme I component/enzyme IIA component
Reads supporting (aligned to +/- strand):  ref base C (107/97);  new base T (32/36);  total (139/134)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 289 total aligned reads displayed.

GACTTCGCCCATTTCTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAA  >  CP009273/2495637‑2495935
                                                                                                                                                     |                                                                                                                                                     
gACTTCGCCCATTTCTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACAtc                                                                                                                                                       >  2:606219/1‑150 (MQ=255)
gACTTCGCCCATTGCTGACGGCCAGCCATGGGGTTTCGCCCGCCGGGCAATGCTGATACTGGAATGCCGGATCCAGTGTGTTTTGGTGTGGGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACAtc                                                                                                                                                       <  1:3731365/150‑1 (MQ=255)
   ttCGCCCTTTTCTGACTGCCAGACACTCGGTTTCGCCTGCCGGGAAATGCTGATACTGTATTGACGGATCCACTTTTATTTGGTGTGGGGTATCGATACGCAAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACAtcttc                                                                                                                                                    <  1:26218/150‑1 (MQ=255)
   ttCGCCCATTTCTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGTAATGACGGATCCACTTATATTTGGTGTGCGGTATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACAtcttc                                                                                                                                                    <  1:154408/150‑1 (MQ=255)
         cATTTCTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAg                                                                                                                                              <  1:1858892/150‑1 (MQ=255)
              cTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCaa                                                                                                                                         >  2:9729/1‑150 (MQ=255)
              cTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCaa                                                                                                                                         >  1:2682084/1‑150 (MQ=255)
                gACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAAc                                                                                                                                       >  1:3488550/1‑150 (MQ=255)
                      cagccaATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGt                                                                                                                                 >  1:2398799/1‑150 (MQ=255)
                            aTCGGTTTCGCCAGCCGGGCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTg                                                                                                                           <  2:9111/150‑1 (MQ=255)
                             tCGGTTTCGCCAGCCGGGCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTgc                                                                                                                          >  1:1152905/1‑150 (MQ=255)
                                gTTTCGCCAGCCGGGCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGcc                                                                                                                       <  1:3594959/150‑1 (MQ=255)
                                 tttCGCCAGCCGGGCAATGCTGATACTGGATTGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGccc                                                                                                                      <  1:2771847/150‑1 (MQ=255)
                                 tttCGCCAGCCGGGCAATGCTGATACTGGAATGACGGATCCACTGAGTTTTGGTGTGGGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGccc                                                                                                                      <  1:535106/150‑1 (MQ=255)
                                 tttCGCCAGCCGGGCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGccc                                                                                                                      <  2:3889700/150‑1 (MQ=255)
                                       cAGCCGGGCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAAc                                                                                                                >  1:3851182/1‑150 (MQ=255)
                                        aGCCGGGCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACg                                                                                                               >  2:4161945/1‑150 (MQ=255)
                                            gggCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGa                                                                                                           <  2:4199890/150‑1 (MQ=255)
                                            gggCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGa                                                                                                           >  1:4349298/1‑150 (MQ=255)
                                                aaTGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGtt                                                                                                       >  2:1334039/1‑150 (MQ=255)
                                                     tGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGc                                                                                                  >  1:1973494/1‑150 (MQ=255)
                                                         acggcattGACGTAACCAGTGAGTTTGGGTGTGGGGTAGCGCTACCCAAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAg                                                                                              <  1:3704596/143‑1 (MQ=255)
                                                         aCTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAg                                                                                              <  2:2792494/150‑1 (MQ=255)
                                                          gtGGAATGAGGGATCCACTGTTTTTTGGTGGGGGGGATCGCTACGCAAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAgg                                                                                             <  1:2520961/149‑1 (MQ=255)
                                                           tgtattGACGGATCCACTGAGTTTGGGGGTGGGGGATCGCTACGCAAAACCCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGa                                                                                            <  1:4130616/145‑1 (MQ=255)
                                                            ggAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGaa                                                                                           >  1:1677819/1‑150 (MQ=255)
                                                            ggAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGaa                                                                                           <  2:2825256/150‑1 (MQ=255)
                                                             taaTGACGGATCCACTTATATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAAc                                                                                          <  1:1469176/149‑1 (MQ=255)
                                                             gAATGACGGATCCACTGAGATTTGGTGTGGGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAAc                                                                                          <  1:506606/150‑1 (MQ=255)
                                                              aaTGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACt                                                                                         >  1:2564483/1‑150 (MQ=255)
                                                                tGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTgg                                                                                       >  2:1468288/1‑150 (MQ=255)
                                                                 gACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGa                                                                                      >  2:4207906/1‑150 (MQ=255)
                                                                 gACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGa                                                                                      >  2:2795350/1‑150 (MQ=255)
                                                                  ccGGACCCCCGGATATTTGGTGTGCGGTATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGAt                                                                                     <  1:1713017/149‑1 (MQ=255)
                                                                  aCGGATCCACTGATATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGAt                                                                                     <  1:645113/150‑1 (MQ=255)
                                                                  aCGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGAt                                                                                     <  2:3079746/150‑1 (MQ=255)
                                                                   cGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATc                                                                                    <  2:1710369/150‑1 (MQ=255)
                                                                    ggATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCg                                                                                   <  1:3909953/150‑1 (MQ=255)
                                                                    ggATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCg                                                                                   <  1:1359849/150‑1 (MQ=255)
                                                                      aTCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGcc                                                                                 <  2:1476032/150‑1 (MQ=255)
                                                                       tCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCt                                                                                >  1:2722936/1‑150 (MQ=255)
                                                                       tCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCt                                                                                <  2:1841039/150‑1 (MQ=255)
                                                                       tCCACTGAGATTTGGGGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCt                                                                                <  1:4684529/150‑1 (MQ=255)
                                                                         cACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGc                                                                              >  2:2444392/1‑150 (MQ=255)
                                                                           gtGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGctc                                                                            >  1:3980613/2‑150 (MQ=255)
                                                                            tGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCGTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGctct                                                                           <  2:487257/150‑1 (MQ=255)
                                                                               tttttGGTGTGCGGGATCGCTACGCAAAAACCACCGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATTTTCTTCAAGATCAACCGGATGTTCAGTGCGCCCTTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTa                                                                        <  1:3403665/148‑1 (MQ=255)
                                                                               gaTTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTa                                                                        <  2:1150704/150‑1 (MQ=255)
                                                                                aTTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTAt                                                                       >  1:2370169/1‑150 (MQ=255)
                                                                                aTTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTAt                                                                       <  2:1175659/150‑1 (MQ=255)
                                                                                 tttGGTGGGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTAtt                                                                      <  2:33835/150‑1 (MQ=255)
                                                                                   tGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGc                                                                    <  2:4033238/150‑1 (MQ=255)
                                                                                    ggTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCt                                                                   >  2:4427547/1‑150 (MQ=255)
                                                                                         gCGGTATCGCTCCGCAAAAACCAACGCCGGTGTTAACAATCTCTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAAt                                                              <  1:473017/150‑1 (MQ=255)
                                                                                          cGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATc                                                             <  2:2537816/150‑1 (MQ=255)
                                                                                           gggATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATcc                                                            >  2:885493/1‑150 (MQ=255)
                                                                                            ggATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCt                                                           <  2:2914137/150‑1 (MQ=255)
                                                                                            ggATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCt                                                           <  2:3626607/150‑1 (MQ=255)
                                                                                            ggATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCt                                                           <  1:1735516/150‑1 (MQ=255)
                                                                                            ggATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCt                                                           >  1:574387/1‑150 (MQ=255)
                                                                                               tCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGAt                                                        >  2:1578521/1‑150 (MQ=255)
                                                                                                 gCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATca                                                      <  2:1069703/150‑1 (MQ=255)
                                                                                                  cTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATcaa                                                     >  1:1772357/1‑150 (MQ=255)
                                                                                                     cGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATcaacaa                                                  <  1:2532490/150‑1 (MQ=255)
                                                                                                      gCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACaaa                                                 <  2:4769537/150‑1 (MQ=255)
                                                                                                      gCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACaaa                                                 >  2:1095709/1‑150 (MQ=255)
                                                                                                      gCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACaaa                                                 >  1:3602645/1‑150 (MQ=255)
                                                                                                           aaCCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGatat                                            <  2:2060278/150‑1 (MQ=255)
                                                                                                             ccAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATtc                                          >  1:713540/1‑150 (MQ=255)
                                                                                                               aaCGCGGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATtctc                                        <  1:4288917/150‑1 (MQ=255)
                                                                                                                 cGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCa                                      >  1:3664251/1‑150 (MQ=255)
                                                                                                                 cGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCa                                      <  2:3825233/150‑1 (MQ=255)
                                                                                                                 cGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGAAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCa                                      <  2:2356842/150‑1 (MQ=255)
                                                                                                                 cGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCa                                      >  1:2986815/1‑150 (MQ=255)
                                                                                                                     ggtggtAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAgcgc                                  >  2:3502344/1‑150 (MQ=255)
                                                                                                                       tggtAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCca                                >  2:754643/1‑150 (MQ=255)
                                                                                                                        ggtAACAATCTCTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCcag                               >  1:1935906/1‑150 (MQ=255)
                                                                                                                         gtAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCcagc                              >  2:3663977/1‑150 (MQ=255)
                                                                                                                          tAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCcagca                             <  1:1079114/150‑1 (MQ=255)
                                                                                                                          tAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCcagca                             <  1:606753/150‑1 (MQ=255)
                                                                                                                           aaCAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCcagcag                            >  2:4479233/1‑150 (MQ=255)
                                                                                                                                tctcTTCCCGCTGCCAGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGc                       >  1:129412/1‑150 (MQ=255)
                                                                                                                                tctcTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGc                       <  2:1864255/150‑1 (MQ=255)
                                                                                                                                 ctctTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCg                      <  2:3724781/150‑1 (MQ=255)
                                                                                                                                      cccGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGt                 >  1:799963/1‑150 (MQ=255)
                                                                                                                                         gCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGtctt              <  1:4608829/150‑1 (MQ=255)
                                                                                                                                           tGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGtcttct            >  2:2301488/1‑150 (MQ=255)
                                                                                                                                           tGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGtcttct            >  2:4594969/1‑150 (MQ=255)
                                                                                                                                           tGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGtcttct            >  1:1626583/1‑150 (MQ=255)
                                                                                                                                            gCCAGAAATGTTCTTCCAGCTCAAACGGATTTTCTGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTcttctt           <  1:3341432/150‑1 (MQ=255)
                                                                                                                                             ccAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCttcttc          >  2:2725598/1‑150 (MQ=255)
                                                                                                                                             ccAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCttcttc          <  2:1973494/150‑1 (MQ=255)
                                                                                                                                              cAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTcc         <  1:3965362/150‑1 (MQ=255)
                                                                                                                                               aGACATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCg        >  1:2070306/1‑150 (MQ=255)
                                                                                                                                                 aCATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGc      >  1:3255263/1‑150 (MQ=255)
                                                                                                                                                 aCATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGc      >  1:825021/1‑150 (MQ=255)
                                                                                                                                                  cATTTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCg     >  2:445368/1‑150 (MQ=255)
                                                                                                                                                    tcttctTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTa   >  2:2397388/1‑150 (MQ=255)
                                                                                                                                                    tcttctTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTa   <  1:995037/150‑1 (MQ=255)
                                                                                                                                                     cttcttCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTaa  <  1:1810095/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACTTCGCCCATTTCTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAA  >  CP009273/2495637‑2495935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A