Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,618,284 C→T 22.3% L628L (CTG→TTG)  ppk → polyphosphate kinase, component of RNA degradosome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,618,2840CT22.3% 579.8 / 162.9 260L628L (CTG→TTG) ppkpolyphosphate kinase, component of RNA degradosome
Reads supporting (aligned to +/- strand):  ref base C (110/92);  new base T (37/21);  total (147/113)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

AACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATT  >  CP009273/2618137‑2618433
                                                                                                                                                   |                                                                                                                                                     
      cGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGAt                                                                                                                                               >  1:2023792/1‑150 (MQ=255)
         gCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCcg                                                                                                                                            >  1:4517541/1‑150 (MQ=255)
          ccATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCcgc                                                                                                                                           >  1:872174/1‑150 (MQ=255)
               aGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTg                                                                                                                                      >  2:3158418/1‑150 (MQ=255)
                 tATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGaa                                                                                                                                    >  1:2503070/1‑150 (MQ=255)
                  aTTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAag                                                                                                                                   >  1:2444429/1‑150 (MQ=255)
                  aTTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAag                                                                                                                                   >  1:39282/1‑150 (MQ=255)
                  aTTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAag                                                                                                                                   <  1:4482975/150‑1 (MQ=255)
                         aCCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCggg                                                                                                                            >  1:2045663/1‑150 (MQ=255)
                         aCCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCggg                                                                                                                            >  1:1949815/1‑150 (MQ=255)
                           cGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTa                                                                                                                          >  1:2693797/1‑150 (MQ=255)
                           cGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTa                                                                                                                          >  1:1341879/1‑150 (MQ=255)
                           cGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTa                                                                                                                          >  2:535972/1‑150 (MQ=255)
                               aCCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTgg                                                                                                                      <  1:4127197/150‑1 (MQ=255)
                                ccTTGAACATGACCGGGTTTATATTTTTGAACATGGCGGCGATACAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGa                                                                                                                     <  2:1165948/150‑1 (MQ=255)
                                ccTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGa                                                                                                                     <  1:4838655/150‑1 (MQ=255)
                                ccTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGa                                                                                                                     <  1:3916587/150‑1 (MQ=255)
                                 cTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGAc                                                                                                                    >  2:4537790/1‑150 (MQ=255)
                                 cTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGAc                                                                                                                    >  2:4203262/1‑150 (MQ=255)
                                      aCATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGAcatcat                                                                                                               <  2:4428737/150‑1 (MQ=255)
                                       cATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACatcatc                                                                                                              >  1:4318157/1‑150 (MQ=255)
                                        aTGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCg                                                                                                             <  2:1938537/150‑1 (MQ=255)
                                        aTGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCg                                                                                                             >  2:2908810/1‑150 (MQ=255)
                                         tGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGa                                                                                                            >  1:2183507/1‑150 (MQ=255)
                                          gACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGAc                                                                                                           <  2:886595/150‑1 (MQ=255)
                                             cGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACata                                                                                                        <  1:2927847/150‑1 (MQ=255)
                                               ggTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATAtt                                                                                                      >  1:4147324/1‑150 (MQ=255)
                                               ggTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATAtt                                                                                                      >  1:342900/1‑150 (MQ=255)
                                                gTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTg                                                                                                     <  1:3847501/150‑1 (MQ=255)
                                                 tttATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGt                                                                                                    >  1:4779057/1‑150 (MQ=255)
                                                 tttATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGt                                                                                                    >  1:4114578/1‑150 (MQ=255)
                                                  ttATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGtt                                                                                                   <  1:1033390/150‑1 (MQ=255)
                                                   tataTTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTc                                                                                                  >  1:759114/1‑150 (MQ=255)
                                                   tataTTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTc                                                                                                  >  1:3190854/1‑150 (MQ=255)
                                                      atTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGc                                                                                               >  2:1501900/1‑150 (MQ=255)
                                                        ttttGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGa                                                                                             >  2:1074319/1‑150 (MQ=255)
                                                         tttGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGAt                                                                                            <  2:2675083/150‑1 (MQ=255)
                                                          ttGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATa                                                                                           <  2:1421446/150‑1 (MQ=255)
                                                             aaaaTGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACgg                                                                                        <  2:3522576/150‑1 (MQ=255)
                                                                 tGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCaa                                                                                    <  2:4147115/150‑1 (MQ=255)
                                                                   gcggcgATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAg                                                                                  <  2:85538/150‑1 (MQ=255)
                                                                     ggcgATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCa                                                                                <  1:1929585/150‑1 (MQ=255)
                                                                     ggcgATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCa                                                                                >  1:1710604/1‑150 (MQ=255)
                                                                        gATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGt                                                                             >  1:4042915/1‑150 (MQ=255)
                                                                            aaaaGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTtata                                                                         <  1:4581085/150‑1 (MQ=255)
                                                                             aaaGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTatat                                                                        <  2:658151/150‑1 (MQ=255)
                                                                              aaGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATc                                                                       >  1:2772579/1‑150 (MQ=255)
                                                                               aGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCg                                                                      <  1:3328123/150‑1 (MQ=255)
                                                                               aGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCg                                                                      <  2:3034660/150‑1 (MQ=255)
                                                                                 gTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGAt                                                                    <  1:130454/150‑1 (MQ=255)
                                                                                  tCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATa                                                                   <  2:3597159/150‑1 (MQ=255)
                                                                                   cTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATaa                                                                  <  2:4269366/150‑1 (MQ=255)
                                                                                   cTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATaa                                                                  >  1:2392088/1‑150 (MQ=255)
                                                                                    tACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATaaa                                                                 >  1:527503/1‑150 (MQ=255)
                                                                                    tACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATaaa                                                                 <  1:4157880/150‑1 (MQ=255)
                                                                                      ccTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGa                                                               <  2:3170301/150‑1 (MQ=255)
                                                                                        tttCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAAc                                                             >  1:3537420/1‑150 (MQ=255)
                                                                                         ttcttcCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACt                                                            <  1:3025842/150‑1 (MQ=255)
                                                                                          tcttcCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTc                                                           >  1:4211347/1‑150 (MQ=255)
                                                                                           cttcCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCa                                                          <  1:792298/150‑1 (MQ=255)
                                                                                              ccgccgACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTa                                                       >  1:1592320/1‑150 (MQ=255)
                                                                                               cgccgACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTaa                                                      >  2:1283053/1‑150 (MQ=255)
                                                                                               cgccgACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTaa                                                      >  1:4085272/1‑150 (MQ=255)
                                                                                                 ccgACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATc                                                    <  1:2640709/150‑1 (MQ=255)
                                                                                                 ccgACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATc                                                    <  1:2640935/150‑1 (MQ=255)
                                                                                                      tGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTAc                                               <  2:4687798/150‑1 (MQ=255)
                                                                                                       ggATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACg                                              <  2:325461/150‑1 (MQ=255)
                                                                                                       ggATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACg                                              <  1:4251584/150‑1 (MQ=255)
                                                                                                         aTGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGtt                                            <  2:1772810/150‑1 (MQ=255)
                                                                                                          tGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTc                                           >  2:3944524/1‑150 (MQ=255)
                                                                                                            aCGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTccc                                         <  1:60970/150‑1 (MQ=255)
                                                                                                             cgcgCAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTcccc                                        <  2:4058091/150‑1 (MQ=255)
                                                                                                              gcgcAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCcg                                       >  2:4098468/1‑150 (MQ=255)
                                                                                                               cgcAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCcgc                                      >  1:3621874/1‑150 (MQ=255)
                                                                                                                 cAATATTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCgg                                    >  1:2964307/1‑150 (MQ=255)
                                                                                                                    tatTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCaa                                 >  1:3699330/1‑150 (MQ=255)
                                                                                                                    tatTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCaa                                 <  2:3890888/150‑1 (MQ=255)
                                                                                                                     atTGATTATCGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAATGCACGGTATATCGAAAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAAt                                >  2:2635524/1‑150 (MQ=255)
                                                                                                                       tGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATcg                              <  2:2348033/150‑1 (MQ=255)
                                                                                                                              cGTATTGAAGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCaaa                       <  1:1523436/150‑1 (MQ=255)
                                                                                                                              cGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCaaa                       <  1:406331/150‑1 (MQ=255)
                                                                                                                              cGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCaaa                       <  1:406298/150‑1 (MQ=255)
                                                                                                                              cGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCaaa                       >  2:2184687/1‑150 (MQ=255)
                                                                                                                               gTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAg                      >  2:2305150/1‑150 (MQ=255)
                                                                                                                                 aTTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTa                    >  2:4499224/1‑150 (MQ=255)
                                                                                                                                 aTTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTa                    >  2:654277/1‑150 (MQ=255)
                                                                                                                                     aaGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACggg                >  2:3686370/1‑150 (MQ=255)
                                                                                                                                     aaGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACggg                >  1:1560724/1‑150 (MQ=255)
                                                                                                                                      aGTGGCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGgc               <  2:3151384/150‑1 (MQ=255)
                                                                                                                                      aGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCTATCGCCGCAAAGTACGGgc               >  2:1022694/1‑150 (MQ=255)
                                                                                                                                      aGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGgc               >  1:2943266/1‑150 (MQ=255)
                                                                                                                                       gTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGgcg              <  1:861583/150‑1 (MQ=255)
                                                                                                                                        tGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGgcgc             >  1:512400/1‑150 (MQ=255)
                                                                                                                                         ggCGACGCCGTTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCa            <  2:4407974/150‑1 (MQ=255)
                                                                                                                                         ggCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCa            >  2:2148848/1‑150 (MQ=255)
                                                                                                                                            gACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGtt         <  1:1211867/150‑1 (MQ=255)
                                                                                                                                             aCGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTg        >  2:1891679/1‑150 (MQ=255)
                                                                                                                                              cgccgcTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTgg       >  2:554696/1‑150 (MQ=255)
                                                                                                                                               gccgcTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGc      <  2:238947/150‑1 (MQ=255)
                                                                                                                                                   ctgctCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGAtt  <  1:108681/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATT  >  CP009273/2618137‑2618433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A