Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,752,877 C→A 27.3% G178G (GGC→GGA)  yfjI → CP4‑57 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,752,8770CA27.3% 289.5 / 109.4 139G178G (GGC→GGA) yfjICP4‑57 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (57/44);  new base A (23/15);  total (80/59)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

Only 100 of 143 total aligned reads displayed.

GTAACAAAGATCACTTGGCAACAAATGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTC  >  CP009273/2752729‑2753026
                                                                                                                                                    |                                                                                                                                                     
gTAACAAAGATCACTTGGCAACAAATGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGca                                                                                                                                                      >  2:1663574/1‑150 (MQ=255)
  aaCAAAGATCACTTGGCAACAAATGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGaatc                                                                                                                                                    >  2:3553240/1‑150 (MQ=255)
    cAAAGATCACTTGGCAACAAATGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGcatcat                                                                                                                                                  <  1:714408/150‑1 (MQ=255)
     aaaGATCACTTGGCAACAAATGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATg                                                                                                                                                 >  1:2036113/1‑150 (MQ=255)
     aaaGATCACTTGGCAACAAATGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATg                                                                                                                                                 >  1:2038222/1‑150 (MQ=255)
     aaaGATCACTTGGCAACAAATGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATg                                                                                                                                                 <  1:3135698/150‑1 (MQ=255)
        gATCACTTGGCAACAAATGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCt                                                                                                                                              >  2:287825/1‑150 (MQ=255)
          tCACTTGGCAACAAATGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCtga                                                                                                                                            >  1:1902756/1‑150 (MQ=255)
           cACTTGGCAACAAATGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCtgat                                                                                                                                           >  1:3372786/1‑150 (MQ=255)
              ttGGCAACAAATGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGaa                                                                                                                                        >  2:1540989/1‑150 (MQ=255)
               tGGCAACAAATGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAg                                                                                                                                       <  2:2475862/150‑1 (MQ=255)
                   aacaaATGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTgg                                                                                                                                   >  1:2313495/1‑150 (MQ=255)
                      aaaTGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGAt                                                                                                                                >  2:1073760/1‑150 (MQ=255)
                      aaaTGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGAt                                                                                                                                >  2:2351406/1‑150 (MQ=255)
                       aaTGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATc                                                                                                                               <  2:636384/150‑1 (MQ=255)
                        aTGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCa                                                                                                                              >  1:3522038/1‑150 (MQ=255)
                        aTGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCa                                                                                                                              <  2:3712319/150‑1 (MQ=255)
                         tGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCAt                                                                                                                             <  1:3781657/150‑1 (MQ=255)
                          gAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCCTGTCTGATGAAGCTGGGATCAtt                                                                                                                            >  2:2439828/1‑150 (MQ=255)
                             aGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCAttttt                                                                                                                         <  2:3867546/150‑1 (MQ=255)
                             aGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCAttttt                                                                                                                         <  2:3868857/150‑1 (MQ=255)
                                    aaGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGtt                                                                                                                  <  2:145649/150‑1 (MQ=255)
                                     aGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTa                                                                                                                 <  2:4550758/150‑1 (MQ=255)
                                      gAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTac                                                                                                                <  2:894051/150‑1 (MQ=255)
                                            ctTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACtt                                                                                                          >  2:2679336/1‑150 (MQ=255)
                                            ctTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGACTCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACtt                                                                                                          >  2:106361/1‑150 (MQ=255)
                                            ctTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACtt                                                                                                          >  2:4219207/1‑150 (MQ=255)
                                              tACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTaa                                                                                                        <  1:4311669/150‑1 (MQ=255)
                                              tACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTaa                                                                                                        <  1:4309251/150‑1 (MQ=255)
                                               aCGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAAc                                                                                                       <  1:3332388/150‑1 (MQ=255)
                                                cGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACg                                                                                                      >  1:3632854/1‑150 (MQ=255)
                                                    aaaCCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCt                                                                                                  >  2:1165429/1‑150 (MQ=255)
                                                    aaaCCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCt                                                                                                  <  1:4021172/150‑1 (MQ=255)
                                                    aaaCCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCt                                                                                                  <  1:3523331/150‑1 (MQ=255)
                                                    aaaCCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCt                                                                                                  >  1:3004927/1‑150 (MQ=255)
                                                    aaaCCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCt                                                                                                  <  1:4021539/150‑1 (MQ=255)
                                                    aaaCCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCt                                                                                                  >  1:3979047/1‑150 (MQ=255)
                                                    aaaCCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCt                                                                                                  >  1:3979271/1‑150 (MQ=255)
                                                    aaaCCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCt                                                                                                  >  1:1314721/1‑150 (MQ=255)
                                                         cGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGt                                                                                             >  2:3314859/1‑150 (MQ=255)
                                                           aaaGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGttt                                                                                           <  1:549541/150‑1 (MQ=255)
                                                            aaGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTa                                                                                          >  1:822739/1‑150 (MQ=255)
                                                                    gTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAg                                                                                  >  2:1168043/1‑150 (MQ=255)
                                                                     tGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGa                                                                                 <  2:4006704/150‑1 (MQ=255)
                                                                     tGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGa                                                                                 <  2:4006583/150‑1 (MQ=255)
                                                                       agaTTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGAtg                                                                               >  2:412527/1‑150 (MQ=255)
                                                                            cAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGa                                                                          >  2:648873/1‑150 (MQ=255)
                                                                             aaaTTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGAt                                                                         >  2:1533947/1‑150 (MQ=255)
                                                                             aaaTTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGAt                                                                         <  2:1164033/150‑1 (MQ=255)
                                                                               aTTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATgg                                                                       <  2:3788287/150‑1 (MQ=255)
                                                                                tttttATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGt                                                                      <  1:1971956/150‑1 (MQ=255)
                                                                                tttttATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGt                                                                      >  1:1575446/1‑150 (MQ=255)
                                                                                       ttaACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAatat                                                               <  1:4792469/150‑1 (MQ=255)
                                                                                           cGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTAc                                                           <  2:3004927/150‑1 (MQ=255)
                                                                                           cGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTAc                                                           <  2:3979271/150‑1 (MQ=255)
                                                                                           cGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTAc                                                           >  2:2394201/1‑150 (MQ=255)
                                                                                               gCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTg                                                       >  1:4472960/1‑150 (MQ=255)
                                                                                                  acacCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTggaa                                                    >  1:4668867/1‑150 (MQ=255)
                                                                                                  acacCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTggaa                                                    <  2:1305881/150‑1 (MQ=255)
                                                                                                    acCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTggaaag                                                  >  1:1674845/1‑150 (MQ=255)
                                                                                                      cTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTggaaagga                                                >  2:17447/1‑150 (MQ=255)
                                                                                                       tGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAGGATGTGGGATGGTTCAATATTTACGGTggaaaggaa                                               >  1:3101114/1‑150 (MQ=255)
                                                                                                       tGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTggaaaggaa                                               <  2:489118/150‑1 (MQ=255)
                                                                                                       tGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTggaaaggaa                                               <  1:4753455/150‑1 (MQ=255)
                                                                                                       tGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTggaaaggaa                                               >  1:1758934/1‑150 (MQ=255)
                                                                                                             tATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGa                                         <  2:1345646/150‑1 (MQ=255)
                                                                                                             tATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGa                                         >  1:1793115/1‑150 (MQ=255)
                                                                                                              aTTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAg                                        >  1:1175657/1‑150 (MQ=255)
                                                                                                              aTTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAg                                        >  2:4874021/1‑150 (MQ=255)
                                                                                                              aTTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAg                                        >  1:4504850/1‑150 (MQ=255)
                                                                                                              aTTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAg                                        >  2:3349670/1‑150 (MQ=255)
                                                                                                              aTTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAg                                        <  1:1111196/150‑1 (MQ=255)
                                                                                                                 aaaGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGccc                                     >  2:2140182/1‑150 (MQ=255)
                                                                                                                 aaaGCTCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGccc                                     >  1:391277/1‑150 (MQ=255)
                                                                                                                   aGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCga                                   <  2:1452941/150‑1 (MQ=255)
                                                                                                                      tCATCTCTGTGGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGaa                                >  2:2134676/1‑150 (MQ=255)
                                                                                                                       cATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGaaa                               >  1:4662631/1‑150 (MQ=255)
                                                                                                                         tctcTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAaatt                             <  2:4689124/150‑1 (MQ=255)
                                                                                                                         tctcTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAaatt                             <  2:4222639/150‑1 (MQ=255)
                                                                                                                         tctcTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAaatt                             <  2:4689034/150‑1 (MQ=255)
                                                                                                                          ctctGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAaatta                            >  1:640361/1‑150 (MQ=255)
                                                                                                                          ctctGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAaatta                            >  1:2730289/1‑150 (MQ=255)
                                                                                                                            ctGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAaattaat                          <  1:2379744/150‑1 (MQ=255)
                                                                                                                             tgtgGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAaattaatt                         >  1:883315/1‑150 (MQ=255)
                                                                                                                             tgtgGGCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAaattaatt                         >  1:883308/1‑150 (MQ=255)
                                                                                                                              gtgGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAattaatta                        >  1:2982752/1‑150 (MQ=255)
                                                                                                                                gggCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTaga                      <  2:2038222/150‑1 (MQ=255)
                                                                                                                                gggCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTaga                      <  2:2036113/150‑1 (MQ=255)
                                                                                                                                gggCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTaga                      >  2:2472806/1‑150 (MQ=255)
                                                                                                                                gggCACTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTaga                      <  1:1073760/150‑1 (MQ=255)
                                                                                                                                   cACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGAt                   >  1:1514269/1‑150 (MQ=255)
                                                                                                                                   cACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGAt                   >  2:398594/1‑150 (MQ=255)
                                                                                                                                    aCTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATg                  >  2:1552951/1‑150 (MQ=255)
                                                                                                                                    aCTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATg                  >  1:1814103/1‑150 (MQ=255)
                                                                                                                                    aCTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATg                  <  2:4447007/150‑1 (MQ=255)
                                                                                                                                    aCTGGCGATCAGTCGGAATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATg                  <  2:4446355/150‑1 (MQ=255)
                                                                                                                                       ggCGATCAGTCGTCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGATAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCaa               <  2:4608752/150‑1 (MQ=255)
                                                                                                                                            tCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATa          >  2:869766/1‑150 (MQ=255)
                                                                                                                                                tCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTGTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAacac      >  1:3802133/1‑150 (MQ=255)
                                                                                                                                                    catcatGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTc  <  2:4774997/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTAACAAAGATCACTTGGCAACAAATGAAAGACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTC  >  CP009273/2752729‑2753026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A