Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,106,398 A→C 23.1% intergenic (‑98/+28) yghF ← / ← yghG pseudogene, secretion pathway protein, C‑type protein homology;putative transport; Not classified; putative general secretion pathway for protein export (GSP)/secretin (GspDbeta) OM localization lipoprotein pilotin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,106,3980AC23.1% 469.9 / 189.3 242intergenic (‑98/+28)yghF/yghGpseudogene, secretion pathway protein, C‑type protein homology;putative transport; Not classified; putative general secretion pathway for protein export (GSP)/secretin (GspDbeta) OM localization lipoprotein pilotin
Reads supporting (aligned to +/- strand):  ref base A (110/76);  new base C (36/20);  total (146/96)
Fisher's exact test for biased strand distribution p-value = 5.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 253 total aligned reads displayed.

AGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAGTGATCATTAATTTCACCGTC  >  CP009273/3106250‑3106546
                                                                                                                                                    |                                                                                                                                                    
 gAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAAcc                                                                                                                                                    >  2:1332941/1‑150 (MQ=255)
 gAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAAcc                                                                                                                                                    >  1:2098752/1‑150 (MQ=255)
  aaaTAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAAccc                                                                                                                                                   >  1:1062203/1‑150 (MQ=255)
  aaaTAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAAccc                                                                                                                                                   <  2:2512737/150‑1 (MQ=255)
    ataataAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCcgc                                                                                                                                                 <  2:2363236/150‑1 (MQ=255)
    ataataAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCcgc                                                                                                                                                 >  2:2856378/1‑150 (MQ=255)
     taataaGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACACCCcgcg                                                                                                                                                >  2:1595693/1‑150 (MQ=255)
      aataaGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCgcgc                                                                                                                                               >  1:16684/1‑150 (MQ=255)
        taaGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCa                                                                                                                                             >  2:3655590/1‑150 (MQ=255)
         aaGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCaa                                                                                                                                            >  1:3476288/1‑150 (MQ=255)
                  cAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCt                                                                                                                                   <  2:3435505/150‑1 (MQ=255)
                   aGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACACCCCGCGCCAATTTATATCTa                                                                                                                                  <  1:1026558/150‑1 (MQ=255)
                    gCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTAc                                                                                                                                 >  1:4620912/1‑150 (MQ=255)
                      cAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTAccc                                                                                                                               <  1:1535612/150‑1 (MQ=255)
                       aGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACACCCCGCGCCAATTTATATCTACCCg                                                                                                                              >  2:3963947/1‑150 (MQ=255)
                       aGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCg                                                                                                                              >  1:2518329/1‑150 (MQ=255)
                       aGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCg                                                                                                                              >  1:2518427/1‑150 (MQ=255)
                       aGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCg                                                                                                                              >  2:309692/1‑150 (MQ=255)
                        gAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGa                                                                                                                             >  1:1852198/1‑150 (MQ=255)
                           cATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAACAACAACCCGCGCCAATTTATATCTACCCGACGt                                                                                                                          >  2:2817841/1‑150 (MQ=255)
                             tCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTa                                                                                                                        <  1:3966264/150‑1 (MQ=255)
                              cccGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACACCCCGCGCCAATTTATATCTACCCGACGTTAt                                                                                                                       <  2:469014/150‑1 (MQ=255)
                              cccGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTAt                                                                                                                       >  1:934326/1‑150 (MQ=255)
                               ccGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATg                                                                                                                      <  1:1114948/150‑1 (MQ=255)
                                     gCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGa                                                                                                                <  1:2645870/150‑1 (MQ=255)
                                      cAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGAc                                                                                                               >  2:4432324/1‑150 (MQ=255)
                                       aaTTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACt                                                                                                              >  2:590853/1‑150 (MQ=255)
                                        aTTTTTCGCAGTTGCTCTTTGTCTTTATTAAGAGATTGTCTCTGGTTAAGTGTGTGACTAATTTTTGTCAGCCATTTAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTa                                                                                                             <  1:4546026/150‑1 (MQ=255)
                                          ttttCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTAtt                                                                                                           <  1:2322475/150‑1 (MQ=255)
                                                          ttGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGAt                                                                                           <  1:4748546/150‑1 (MQ=255)
                                                            gTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATcc                                                                                         >  1:1353335/1‑150 (MQ=255)
                                                            gTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATcc                                                                                         >  1:2500064/1‑150 (MQ=255)
                                                                 tATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACACCCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGttt                                                                                    >  1:642941/1‑150 (MQ=255)
                                                                  aTTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACACCCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTc                                                                                   <  1:3262458/150‑1 (MQ=255)
                                                                  aTTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTc                                                                                   >  2:1376661/1‑150 (MQ=255)
                                                                  aTTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTc                                                                                   >  2:3835353/1‑150 (MQ=255)
                                                                   ttAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACACCCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTcc                                                                                  >  2:2151/1‑150 (MQ=255)
                                                                    tAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCg                                                                                 >  2:2713537/1‑150 (MQ=255)
                                                                     aaGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGc                                                                                >  1:2135962/1‑150 (MQ=255)
                                                                       gagaTTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACACCCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTg                                                                              <  1:3013036/150‑1 (MQ=255)
                                                                        agaTTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGa                                                                             >  2:798159/1‑150 (MQ=255)
                                                                          aTTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACACCCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATa                                                                           <  2:4548388/150‑1 (MQ=255)
                                                                          aTTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATa                                                                           >  1:4369156/1‑150 (MQ=255)
                                                                             gTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTg                                                                        >  2:933315/1‑150 (MQ=255)
                                                                                tctGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGtt                                                                     >  2:203994/1‑150 (MQ=255)
                                                                                tctGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGtt                                                                     >  2:461103/1‑150 (MQ=255)
                                                                                  tGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTAc                                                                   >  1:1478318/1‑150 (MQ=255)
                                                                                  tGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTAc                                                                   >  2:2244258/1‑150 (MQ=255)
                                                                                      tAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGt                                                               >  1:1058270/1‑150 (MQ=255)
                                                                                       aaGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTa                                                              >  1:1084875/1‑150 (MQ=255)
                                                                                       aaGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTa                                                              >  2:4570848/1‑150 (MQ=255)
                                                                                       aaGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCCACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTa                                                              <  1:3725856/150‑1 (MQ=255)
                                                                                           gtgtGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACgtg                                                          <  1:4124448/150‑1 (MQ=255)
                                                                                           gtgtGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACgtg                                                          <  2:3274113/150‑1 (MQ=255)
                                                                                            tgtgACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACACCCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACATGTACgtgt                                                         >  2:1888622/1‑150 (MQ=255)
                                                                                             gtgACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTa                                                        >  1:4426446/1‑150 (MQ=255)
                                                                                              tgACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTAt                                                       <  2:316500/150‑1 (MQ=255)
                                                                                              tgACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTAt                                                       <  2:1985736/150‑1 (MQ=255)
                                                                                              tgACGAATTTTTGTCAGCCAGTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTAt                                                       <  2:4816788/150‑1 (MQ=255)
                                                                                                 cGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCAt                                                    >  2:4165891/1‑150 (MQ=255)
                                                                                                  gAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACACCCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCAtt                                                   >  1:990023/1‑150 (MQ=255)
                                                                                                  gAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCAtt                                                   >  1:2932290/1‑150 (MQ=255)
                                                                                                  gAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCAtt                                                   <  1:3188824/150‑1 (MQ=255)
                                                                                                   aaTTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTa                                                  <  2:1606237/150‑1 (MQ=255)
                                                                                                   aaTTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTa                                                  <  2:4318734/150‑1 (MQ=255)
                                                                                                     tttttGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACACCCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAAt                                                >  1:376868/1‑150 (MQ=255)
                                                                                                      ttttGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATc                                               <  1:3655590/150‑1 (MQ=255)
                                                                                                      ttttGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATc                                               <  1:3434991/150‑1 (MQ=255)
                                                                                                      ttttGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATc                                               <  2:1062203/150‑1 (MQ=255)
                                                                                                         tGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGtt                                            >  2:4251441/1‑150 (MQ=255)
                                                                                                           tCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTAt                                          <  1:718125/150‑1 (MQ=255)
                                                                                                             aGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTGTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGc                                        >  1:1286641/1‑150 (MQ=255)
                                                                                                              gCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCt                                       >  1:476008/1‑150 (MQ=255)
                                                                                                               ccATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTg                                      <  2:3614796/150‑1 (MQ=255)
                                                                                                                cATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGt                                     >  1:1037984/1‑150 (MQ=255)
                                                                                                                    gAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGtaatt                                 >  2:571357/1‑150 (MQ=255)
                                                                                                                     aaTGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGtaatta                                <  1:2769776/150‑1 (MQ=255)
                                                                                                                      aTGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGtaattaa                               >  2:3811907/1‑150 (MQ=255)
                                                                                                                      aTGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCCGTACGTGTATCATTAATCGTTATGCTGtaattaa                               >  2:171217/1‑150 (MQ=255)
                                                                                                                         agaTAAATTCGTGCGTCACGAAAAACACCCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTc                            >  2:4045253/1‑150 (MQ=255)
                                                                                                                         agaTAAATTCGTGCGTCACGAAAAACACCCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTc                            >  2:4045355/1‑150 (MQ=255)
                                                                                                                         agaTAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTc                            <  1:2397941/150‑1 (MQ=255)
                                                                                                                         agaTAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTc                            >  1:3905236/1‑150 (MQ=255)
                                                                                                                             aaaTTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTc                        >  1:4064867/1‑150 (MQ=255)
                                                                                                                               aTTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAg                      >  1:3126550/1‑150 (MQ=255)
                                                                                                                                  cGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAGTGa                   <  1:4320114/150‑1 (MQ=255)
                                                                                                                                   gTGCGTCACGAAAAACACCCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAGTGAt                  <  1:1916292/150‑1 (MQ=255)
                                                                                                                                   gTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAGTGAt                  >  2:3495389/1‑150 (MQ=255)
                                                                                                                                   gTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAGTGAt                  >  2:3554494/1‑150 (MQ=255)
                                                                                                                                    tGCGTCACGAAAAACACCCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAGTGATc                 >  1:4039728/1‑150 (MQ=255)
                                                                                                                                         cACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAGTGATCATTaa            >  1:4197747/1‑150 (MQ=255)
                                                                                                                                            gAAAAACACCCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAGTGATCATTAAttt         >  2:3939294/1‑150 (MQ=255)
                                                                                                                                            gAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAGTGATCATTAAttt         <  1:2713500/150‑1 (MQ=255)
                                                                                                                                             aaaaaCAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAGTGATCATTAATTTc        >  2:1286795/1‑150 (MQ=255)
                                                                                                                                              aaaaCACCCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAGTGATCATTAATTTCa       >  2:2330764/1‑150 (MQ=255)
                                                                                                                                              aaaaCACCCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAGTGATCATTAATTTCa       >  2:2330731/1‑150 (MQ=255)
                                                                                                                                               aaaCAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAGTGATCATTAATTTCAc      >  1:461887/1‑150 (MQ=255)
                                                                                                                                                   aacCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAGTGATCATTAATTTCACCGTc  >  1:1463513/1‑150 (MQ=255)
                                                                                                                                                   aacCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAGTGATCATTAATTTCACCGTc  >  2:1357873/1‑150 (MQ=255)
                                                                                                                                                   aacCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAGTGATCATTAATTTCACCGTc  <  2:3771897/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTGGTTAAGAGTGTGACGAATTTTTGTCAGCCATTGAATGAGATAAATTCGTGCGTCACGAAAAACAACCCGCGCCAATTTATATCTACCCGACGTTATGCTTTGACTATTCCACAGGTGGTACGATCCAGTTTCCGCTGATACTGGTTACCTGTACGTGTATCATTAATCGTTATGCTGTAATTAATTCCCTCAGTGATCATTAATTTCACCGTC  >  CP009273/3106250‑3106546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A