Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,169,378 G→T 21.3% S272* (TCA→TAA)  cpdA ← 3',5' cAMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,169,3780GT21.3% 748.3 / 164.6 277S272* (TCA→TAA) cpdA3',5' cAMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (108/110);  new base T (30/29);  total (138/139)
Fisher's exact test for biased strand distribution p-value = 8.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 280 total aligned reads displayed.

TCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTC  >  CP009273/3169229‑3169527
                                                                                                                                                     |                                                                                                                                                     
 cGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGa                                                                                                                                                      <  1:2724873/150‑1 (MQ=255)
 cGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGa                                                                                                                                                      >  2:369820/1‑150 (MQ=255)
   gAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTTAAg                                                                                                                                                    <  1:338329/150‑1 (MQ=255)
    aaGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGc                                                                                                                                                   >  1:4052675/1‑150 (MQ=255)
    aaGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGc                                                                                                                                                   >  2:1299878/1‑150 (MQ=255)
     aGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTTAAGCg                                                                                                                                                  <  1:2410492/150‑1 (MQ=255)
        aTACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTa                                                                                                                                               <  2:3494900/150‑1 (MQ=255)
          aCGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATc                                                                                                                                             >  2:3330131/1‑150 (MQ=255)
          aCGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATc                                                                                                                                             <  1:1310757/150‑1 (MQ=255)
            ggcggcAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAg                                                                                                                                           >  1:2714804/1‑150 (MQ=255)
               ggcAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTTAAGCGGTATCAGGtt                                                                                                                                        >  2:2068116/1‑150 (MQ=255)
                gcAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTTAAGCGGTATCAGGTTg                                                                                                                                       <  1:1408904/150‑1 (MQ=255)
                  aaCTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGa                                                                                                                                     >  1:3131471/1‑150 (MQ=255)
                   aCTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTACATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGaa                                                                                                                                    >  2:4710670/1‑150 (MQ=255)
                   aCTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGaa                                                                                                                                    >  1:2317280/1‑150 (MQ=255)
                    cTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGaaa                                                                                                                                   <  1:2108980/150‑1 (MQ=255)
                    cTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGaaa                                                                                                                                   <  1:362084/150‑1 (MQ=255)
                     tGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAAc                                                                                                                                  <  2:1524376/150‑1 (MQ=255)
                      gCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACg                                                                                                                                 <  2:1973337/150‑1 (MQ=255)
                       cGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAAAgt                                                                                                                                >  1:3490271/1‑150 (MQ=255)
                        ggAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGTGGTATCAGGTTGGAAACgtg                                                                                                                               >  2:327830/1‑150 (MQ=255)
                         gAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACgtgt                                                                                                                              <  1:2061435/150‑1 (MQ=255)
                         gAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACgtgt                                                                                                                              <  1:2762957/150‑1 (MQ=255)
                          aaTGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTTAAGCGGTATCAGGTTGGAAACgtgtg                                                                                                                             >  1:819248/1‑150 (MQ=255)
                          aaTGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACgtgtg                                                                                                                             >  2:3148793/1‑150 (MQ=255)
                           aTGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACgtgtgt                                                                                                                            <  2:4452/150‑1 (MQ=255)
                             gATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTcc                                                                                                                          <  2:4837932/150‑1 (MQ=255)
                              atcatcTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTTAAGCGGTATCAGGTTGGAAACGTGTGTCCg                                                                                                                         >  1:3162549/1‑150 (MQ=255)
                                   ctcAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAgg                                                                                                                    >  2:1772440/1‑150 (MQ=255)
                                   ctcAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAgg                                                                                                                    <  2:2032962/150‑1 (MQ=255)
                                     cAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCg                                                                                                                  >  1:1705205/1‑150 (MQ=255)
                                     cAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCg                                                                                                                  >  1:1717587/1‑150 (MQ=255)
                                      aaCGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGa                                                                                                                 >  2:1855706/1‑150 (MQ=255)
                                        cGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATg                                                                                                               <  1:1494238/150‑1 (MQ=255)
                                           cAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCAc                                                                                                            >  2:3844540/1‑150 (MQ=255)
                                             gggTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTTAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCt                                                                                                          <  1:1753271/150‑1 (MQ=255)
                                             gggTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCt                                                                                                          <  1:1997226/150‑1 (MQ=255)
                                              ggTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTc                                                                                                         >  1:4630484/1‑150 (MQ=255)
                                                tGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCgg                                                                                                       <  2:42058/150‑1 (MQ=255)
                                                  aTGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTTAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCggtg                                                                                                     <  1:3349136/150‑1 (MQ=255)
                                                  aTGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCggtg                                                                                                     >  2:2030231/1‑150 (MQ=255)
                                                      tCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCa                                                                                                 <  1:624732/150‑1 (MQ=255)
                                                       ccGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAg                                                                                                <  1:3767886/150‑1 (MQ=255)
                                                       ccGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAg                                                                                                <  2:2767037/150‑1 (MQ=255)
                                                        cGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGc                                                                                               <  2:4326642/150‑1 (MQ=255)
                                                          ccagccagTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGt                                                                                             >  2:2317728/1‑150 (MQ=255)
                                                             gccagTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTTAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGcc                                                                                          >  1:1560034/1‑150 (MQ=255)
                                                             gccagTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGcc                                                                                          >  2:2861390/1‑150 (MQ=255)
                                                              ccagTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTTAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCa                                                                                         >  1:2918956/1‑150 (MQ=255)
                                                              ccagTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTTAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCa                                                                                         <  2:4095746/150‑1 (MQ=255)
                                                              ccagTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCa                                                                                         <  1:905244/150‑1 (MQ=255)
                                                              ccagTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCa                                                                                         <  2:3625744/150‑1 (MQ=255)
                                                               cagTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCAt                                                                                        <  2:2227533/150‑1 (MQ=255)
                                                                 gTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTTAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCa                                                                                      <  2:534512/150‑1 (MQ=255)
                                                                  ttttttAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTTAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAg                                                                                     >  1:3557011/1‑150 (MQ=255)
                                                                    ttttAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCa                                                                                   >  2:3901432/1‑150 (MQ=255)
                                                                      ttAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATg                                                                                 >  2:503475/1‑150 (MQ=255)
                                                                      ttAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATg                                                                                 <  2:3394897/150‑1 (MQ=255)
                                                                       tAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGt                                                                                >  1:2683858/1‑150 (MQ=255)
                                                                        aaCAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTa                                                                               >  2:2574312/1‑150 (MQ=255)
                                                                        aaCAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTa                                                                               <  2:4058935/150‑1 (MQ=255)
                                                                            aGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTc                                                                           >  1:1202134/1‑150 (MQ=255)
                                                                              cTCGCTGTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCga                                                                         <  2:4764032/150‑1 (MQ=255)
                                                                               tCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCgag                                                                        <  1:4438810/150‑1 (MQ=255)
                                                                                  cTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGt                                                                     <  1:277483/150‑1 (MQ=255)
                                                                                     ttGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACg                                                                  >  2:1016216/1‑150 (MQ=255)
                                                                                        cAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCa                                                               <  1:2460602/150‑1 (MQ=255)
                                                                                        cAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCa                                                               <  1:2540162/150‑1 (MQ=255)
                                                                                         agagCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTTAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAg                                                              >  1:4130670/1‑150 (MQ=255)
                                                                                               cggcgAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTTAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGgc                                                        >  2:1869813/1‑150 (MQ=255)
                                                                                                   gatcTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTTAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGa                                                    <  1:2624182/147‑1 (MQ=255)
                                                                                                   gAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGa                                                    >  2:3834731/1‑150 (MQ=255)
                                                                                                   gAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGa                                                    >  2:3833808/1‑150 (MQ=255)
                                                                                                    aGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGAt                                                   >  2:1415031/1‑150 (MQ=255)
                                                                                                     gCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATg                                                  <  1:4367817/150‑1 (MQ=255)
                                                                                                      cTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATgg                                                 >  1:1048137/1‑150 (MQ=255)
                                                                                                       tGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGt                                                >  1:1447048/1‑150 (MQ=255)
                                                                                                          tGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATc                                             >  1:3957643/1‑150 (MQ=255)
                                                                                                                 gtgtAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTa                                      <  1:4672035/150‑1 (MQ=255)
                                                                                                                 gtgtAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTa                                      >  1:2266332/1‑150 (MQ=255)
                                                                                                                      aataaaGAAGCGTAGACATCAGTAGCCTTCTTAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGtt                                 >  2:3963922/1‑150 (MQ=255)
                                                                                                                       ataaaGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTg                                >  1:4649069/1‑150 (MQ=255)
                                                                                                                       ataaaGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTg                                >  1:635945/1‑150 (MQ=255)
                                                                                                                         aaaGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGa                              >  1:3629199/1‑150 (MQ=255)
                                                                                                                              agCGTAGACATCAGTAGCCTTCTTAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGt                         >  1:2165065/1‑150 (MQ=255)
                                                                                                                              agCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGt                         <  1:4739989/150‑1 (MQ=255)
                                                                                                                               gCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTg                        <  2:4533/150‑1 (MQ=255)
                                                                                                                               gCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTg                        <  2:4052522/150‑1 (MQ=255)
                                                                                                                                cGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGc                       <  1:3876010/150‑1 (MQ=255)
                                                                                                                                 gTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCg                      >  1:1132216/1‑150 (MQ=255)
                                                                                                                                 gTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCg                      >  1:3604030/1‑150 (MQ=255)
                                                                                                                                  tAGACATCAGTAGCCTTCTTAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAATAGTGCgg                     >  1:2242004/1‑150 (MQ=255)
                                                                                                                                    gACATCAGTAGCCTTCTTAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCt                   <  2:730271/150‑1 (MQ=255)
                                                                                                                                          aGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACt             >  1:1180329/1‑150 (MQ=255)
                                                                                                                                           gTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTg            >  2:2657378/1‑150 (MQ=255)
                                                                                                                                            tAGCCTTCTTAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGc           >  2:2465993/1‑150 (MQ=255)
                                                                                                                                            tAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGc           >  2:4296105/1‑150 (MQ=255)
                                                                                                                                             aGCCTTCTTAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGca          <  2:819248/150‑1 (MQ=255)
                                                                                                                                             aGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGca          >  2:2444598/1‑150 (MQ=255)
                                                                                                                                                cTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGcacac       >  1:1840595/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTC  >  CP009273/3169229‑3169527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A