Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,507,749 G→T 48.8% H224N (CAT→AAT)  rpe ← D‑ribulose‑5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,507,7490GT48.8% 114.3 / inf 307H224N (CAT→AAT) rpeD‑ribulose‑5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (64/93);  new base T (73/77);  total (137/170)
Fisher's exact test for biased strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 307 total aligned reads displayed.

TCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTT  >  CP009273/3507601‑3507895
                                                                                                                                                    |                                                                                                                                                  
tCCAGTTAATAACGCTTTCTTCACCTGCGACGGGCAACACCAGCGCATACAGCGCAATATCTACCGCAGCACCAAGACCAGCACCACTGTCGACCAGCTTACCAACAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGa                                                                                                                                                   <  2:4527108/150‑1 (MQ=255)
tCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGa                                                                                                                                                   <  2:4402949/150‑1 (MQ=255)
  cAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACt                                                                                                                                                 >  1:74766/1‑150 (MQ=255)
      taataaCGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTAcc                                                                                                                                             <  2:137147/150‑1 (MQ=255)
        ataaCGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCtt                                                                                                                                           >  2:3869236/1‑150 (MQ=255)
         taaCGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCttt                                                                                                                                          >  1:2431865/1‑150 (MQ=255)
          accgcgTTCTTCACCTGCGACGGTCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAATATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTg                                                                                                                                         <  1:1854502/148‑1 (MQ=255)
          aaCGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTg                                                                                                                                         >  1:2213704/1‑150 (MQ=255)
             gggTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCTGGAGCACTGTCGACCAGCGTACCATCAAGATCAAACGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCa                                                                                                                                      <  1:3729948/148‑1 (MQ=255)
             gcgTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCACGACCCGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCa                                                                                                                                      <  2:4784577/150‑1 (MQ=255)
             gcgTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCa                                                                                                                                      >  1:1752874/1‑150 (MQ=255)
              cgTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAg                                                                                                                                     >  1:3755032/1‑150 (MQ=255)
               gTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGt                                                                                                                                    <  2:761421/150‑1 (MQ=255)
                  cttcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCa                                                                                                                                 >  2:2695035/1‑150 (MQ=255)
                  cttcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGAACAAACGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCa                                                                                                                                 <  1:947884/150‑1 (MQ=255)
                    tcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACt                                                                                                                               <  2:1162003/150‑1 (MQ=255)
                    tcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACt                                                                                                                               <  2:2603881/150‑1 (MQ=255)
                    tcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACt                                                                                                                               >  1:4388302/1‑150 (MQ=255)
                    tcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACt                                                                                                                               <  2:3622628/150‑1 (MQ=255)
                    tcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACt                                                                                                                               >  1:2375450/1‑150 (MQ=255)
                     cACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTg                                                                                                                              >  1:4783415/1‑150 (MQ=255)
                     cACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTg                                                                                                                              <  2:728503/150‑1 (MQ=255)
                      aCCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTgc                                                                                                                             >  1:1676062/1‑150 (MQ=255)
                          gcgcCGGGCAACTCCAGCGAATACAGCGCCATATCTACCGCCGCAGCAAGACCAGTAGCACTGTCGACCCGCGTACCATCAACAAAAAACGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAt                                                                                                                         <  1:1268346/150‑1 (MQ=255)
                            gACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAttt                                                                                                                       <  2:3787445/150‑1 (MQ=255)
                            gACGGGCAACTCCAGCGAATACAGCGCAATATCTCCCCCAGAAGAAACACCAGGAGCACTGTCGACCAGCTTACAAAAAAGAAAAAACGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAttt                                                                                                                       <  1:2928707/150‑1 (MQ=255)
                            gACGGGAAACTCCAGCGCATACAGCGCAATATCTACCGCAGCAGAAAGACCAGGAGCACTGTCGACCAGCGTACAATCAATAACAAACGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAttt                                                                                                                       <  1:665614/150‑1 (MQ=255)
                              cGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTtca                                                                                                                     >  1:1124399/1‑150 (MQ=255)
                               gggCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCGGGAGCACTGTCGACCAGCGTACCATCAAGAACAAACGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTtcat                                                                                                                    <  1:1488998/150‑1 (MQ=255)
                               gggCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCTTACCATCAAGAAAAAACGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTtcat                                                                                                                    <  1:1662547/150‑1 (MQ=255)
                                ggCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTtcatc                                                                                                                   >  2:1828442/1‑150 (MQ=255)
                                 gCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTAGTCATGACTTACCTTTGCCAGTTCACTGCGCATTtcatca                                                                                                                  <  2:1691157/150‑1 (MQ=255)
                                    aCTCCAGCGCAAACCGCGCAATATCTACCGCAGCAGCAAGACCAGGACCACTTCCCACCAGCTAACCACAAACAACAAACGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATg                                                                                                               <  1:125/150‑1 (MQ=255)
                                     cTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGa                                                                                                              >  1:4035159/1‑150 (MQ=255)
                                     cTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGa                                                                                                              >  2:3098032/1‑150 (MQ=255)
                                     cTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGa                                                                                                              >  2:622271/1‑150 (MQ=255)
                                         aGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAACGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACttt                                                                                                          <  1:2284015/150‑1 (MQ=255)
                                          gcgcATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACtttt                                                                                                         <  2:3187727/150‑1 (MQ=255)
                                           cgcATACAGCGCCATATCTCCCGCAGCTGAAAGACCAGGAGCAGTGTCGACCCGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACttttt                                                                                                        <  1:4493217/150‑1 (MQ=255)
                                            tcATACAGCGCAATATCTACCGCAGCAGAAAGACCAGGAGCATTGTCGACCAGCGTACCATCAAGAAAAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACtttttt                                                                                                       <  1:3384178/149‑1 (MQ=255)
                                            gaaTACAGCTCCATATCTACCGCTTCAGCAAGACCTGTATCACTGTCGACCAGGGTACCATCATGATAAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACtttttt                                                                                                       <  2:913677/148‑1 (MQ=255)
                                             cATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTg                                                                                                      >  2:4354185/1‑150 (MQ=255)
                                                 cAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATAAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGt                                                                                                  <  1:3179966/150‑1 (MQ=255)
                                                   gcgcCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTct                                                                                                <  2:1608156/150‑1 (MQ=255)
                                                     gcCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctgg                                                                                              <  2:3487552/150‑1 (MQ=255)
                                                     gcCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctgg                                                                                              >  1:4621073/1‑150 (MQ=255)
                                                      ccATATCTACCGCAGCAGCAAGACCAGGAGCACTTTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctggc                                                                                             <  2:698174/150‑1 (MQ=255)
                                                        atatCTACCGCAGCAGCAATCCCAGGAGCACTGTCGACCAGCGTACAATCAAGAACAACAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctggctg                                                                                           <  1:2106641/150‑1 (MQ=255)
                                                          atCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGt                                                                                         >  2:447901/1‑150 (MQ=255)
                                                             tACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGa                                                                                      <  1:1568196/150‑1 (MQ=255)
                                                               ccGCAGCAGCAAGACCAGGAGCACGGTCGACCAGCGTACCATCAAGAACAACCGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAg                                                                                    <  1:550766/150‑1 (MQ=255)
                                                                   agcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTg                                                                                >  2:85359/1‑150 (MQ=255)
                                                                   agcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTg                                                                                >  1:3513394/1‑150 (MQ=255)
                                                                         aataCCAGGAGCAGGTTCGACCAGCGTACCATCAAGAACAAACGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAAc                                                                          <  1:413564/147‑1 (MQ=255)
                                                                             ccAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGc                                                                      <  2:735553/150‑1 (MQ=255)
                                                                              cAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcg                                                                     >  1:4760458/1‑150 (MQ=255)
                                                                              cAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcg                                                                     <  2:2378781/150‑1 (MQ=255)
                                                                               aGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcga                                                                    >  2:1034893/1‑150 (MQ=255)
                                                                                   gCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGaa                                                                <  2:529669/150‑1 (MQ=255)
                                                                                     aCTGTCGACCAGCGTACCATCAAGATCAAAAGCGATGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACa                                                              <  1:489252/150‑1 (MQ=255)
                                                                                       tGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACata                                                            >  2:3993915/1‑150 (MQ=255)
                                                                                       tGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCATTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACata                                                            <  2:2890601/150‑1 (MQ=255)
                                                                                         tCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATc                                                          <  2:3336315/150‑1 (MQ=255)
                                                                                         tCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATc                                                          >  1:179612/1‑150 (MQ=255)
                                                                                          cGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATcc                                                         >  1:2643415/1‑150 (MQ=255)
                                                                                          cGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTGACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATcc                                                         <  2:676928/150‑1 (MQ=255)
                                                                                           gACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCcg                                                        >  1:2043254/1‑150 (MQ=255)
                                                                                           gACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCcg                                                        <  2:4566144/150‑1 (MQ=255)
                                                                                              cAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCgcgc                                                     >  1:2056215/1‑150 (MQ=255)
                                                                                                 cGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcccg                                                  <  2:1801898/150‑1 (MQ=255)
                                                                                                  gTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcccgc                                                 >  1:1051909/1‑150 (MQ=255)
                                                                                                   tACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcccgcc                                                <  2:4176904/150‑1 (MQ=255)
                                                                                                      cATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCa                                             >  1:3454781/1‑150 (MQ=255)
                                                                                                          aaGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGa                                         >  1:3796878/1‑150 (MQ=255)
                                                                                                          aaGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCCGCGa                                         >  2:1518847/1‑150 (MQ=255)
                                                                                                           aGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGAt                                        >  2:4729458/1‑150 (MQ=255)
                                                                                                            gATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGAtt                                       <  1:4333115/150‑1 (MQ=255)
                                                                                                             aacAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGAttt                                      <  1:2270161/148‑1 (MQ=255)
                                                                                                               cAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCg                                    <  1:1402856/150‑1 (MQ=255)
                                                                                                                   aGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCaa                                >  1:3023233/1‑150 (MQ=255)
                                                                                                                    gCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAt                               <  2:1938328/150‑1 (MQ=255)
                                                                                                                      gACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAtgt                             >  2:2222034/1‑150 (MQ=255)
                                                                                                                        cgccgcGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAtgttg                           <  1:230943/150‑1 (MQ=255)
                                                                                                                          ccgcGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATgttgtt                         >  2:2162015/1‑150 (MQ=255)
                                                                                                                           cgcgAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTc                        >  1:3268012/1‑150 (MQ=255)
                                                                                                                                atatCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCtt                   >  1:1005682/1‑150 (MQ=255)
                                                                                                                                atatCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGAATTTCATCAATGACTTTTTTGTAGTATGGCTGGTCGAAGATTGCCGAACAGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCtt                   >  1:272555/1‑150 (MQ=255)
                                                                                                                                  atCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCa                 <  2:1433987/150‑1 (MQ=255)
                                                                                                                                  atCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCa                 <  2:2963750/150‑1 (MQ=255)
                                                                                                                                      tCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGcc             >  1:910958/1‑150 (MQ=255)
                                                                                                                                        aaaCTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCAc           >  1:1211813/1‑150 (MQ=255)
                                                                                                                                          aCTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCg         >  1:3946712/1‑150 (MQ=255)
                                                                                                                                            ttATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTc       >  1:1628097/1‑150 (MQ=255)
                                                                                                                                             tATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTcc      >  2:1234884/1‑150 (MQ=255)
                                                                                                                                             tATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTcc      <  1:2879014/150‑1 (MQ=255)
                                                                                                                                             tATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTcc      <  1:4431747/150‑1 (MQ=255)
                                                                                                                                              aTTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCa     >  1:3153666/1‑150 (MQ=255)
                                                                                                                                               ttCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCAc    <  1:85359/150‑1 (MQ=255)
                                                                                                                                               ttCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTTCAGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGAGTTCGCCAATGTTGATCACCTTCACTCCACCGACCAc    >  2:428834/1‑150 (MQ=255)
                                                                                                                                                tCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACt   >  1:2330189/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTT  >  CP009273/3507601‑3507895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A