Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,555,311 T→C 28.1% intergenic (‑128/‑62) glpE ← / → glpD thiosulfate:cyanide sulfurtransferase (rhodanese)/sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,555,3110TC28.1% 327.4 / 164.3 196intergenic (‑128/‑62)glpE/glpDthiosulfate:cyanide sulfurtransferase (rhodanese)/sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (82/59);  new base C (36/19);  total (118/78)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 199 total aligned reads displayed.

TAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGTTTATCCGTGC  >  CP009273/3555162‑3555460
                                                                                                                                                     |                                                                                                                                                     
tAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGAtt                                                                                                                                                       >  2:3309603/1‑150 (MQ=255)
  aTACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCt                                                                                                                                                     <  1:2968362/150‑1 (MQ=255)
     cATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTg                                                                                                                                                  <  1:606629/150‑1 (MQ=255)
      aTTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGc                                                                                                                                                 >  2:2838454/1‑150 (MQ=255)
      aTTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGc                                                                                                                                                 >  1:4325648/1‑150 (MQ=255)
         cGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTaa                                                                                                                                              >  2:3134460/1‑150 (MQ=255)
            aCTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAatat                                                                                                                                           <  2:2668687/150‑1 (MQ=255)
              tGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGt                                                                                                                                         <  2:2089645/150‑1 (MQ=255)
               gATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGTTTCTTTGCTAATATGtt                                                                                                                                        >  2:4481669/1‑150 (MQ=255)
                 tCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCg                                                                                                                                      >  2:4041566/1‑150 (MQ=255)
                   cATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGAt                                                                                                                                    >  2:2829571/1‑150 (MQ=255)
                     tGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATaa                                                                                                                                  <  1:2900895/150‑1 (MQ=255)
                     tGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATaa                                                                                                                                  >  2:1549534/1‑150 (MQ=255)
                       cGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACg                                                                                                                                >  2:3441962/1‑150 (MQ=255)
                         tctctcTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGaa                                                                                                                              >  1:3773707/1‑150 (MQ=255)
                         tctctcTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGaa                                                                                                                              >  2:4312495/1‑150 (MQ=255)
                         tctctcTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGaa                                                                                                                              >  1:1310573/1‑150 (MQ=255)
                         tctctcTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGaa                                                                                                                              >  2:406461/1‑150 (MQ=255)
                          ctctctTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAAc                                                                                                                             <  1:3097529/150‑1 (MQ=255)
                           tctctTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACa                                                                                                                            <  2:4196029/150‑1 (MQ=255)
                           tctctTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACa                                                                                                                            <  2:1498487/150‑1 (MQ=255)
                            ctctTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACAt                                                                                                                           >  2:3580794/1‑150 (MQ=255)
                                     tACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGACCATTTATGAGCt                                                                                                                  >  1:210600/1‑150 (MQ=255)
                                      acaaacaaGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCtt                                                                                                                 <  2:415771/150‑1 (MQ=255)
                                      acaaacaaGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCtt                                                                                                                 <  2:2848159/150‑1 (MQ=255)
                                       caaacaaGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCttt                                                                                                                <  2:3916358/150‑1 (MQ=255)
                                            aaGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGa                                                                                                           >  2:1377812/1‑150 (MQ=255)
                                              gTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGaaa                                                                                                         <  2:2047084/150‑1 (MQ=255)
                                              gTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGaaa                                                                                                         >  1:3689935/1‑150 (MQ=255)
                                               tGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAg                                                                                                        >  2:174349/1‑150 (MQ=255)
                                               tGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAg                                                                                                        >  1:1526091/1‑150 (MQ=255)
                                                gggCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGt                                                                                                       <  1:1935132/150‑1 (MQ=255)
                                                   cAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGaa                                                                                                    <  2:4311137/150‑1 (MQ=255)
                                                    aaaTTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAAt                                                                                                   >  1:163518/1‑150 (MQ=255)
                                                    aaaTTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAAt                                                                                                   >  1:164067/1‑150 (MQ=255)
                                                    aaaTTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAAt                                                                                                   >  1:3148737/1‑150 (MQ=255)
                                                     aaTTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATg                                                                                                  <  2:4647632/150‑1 (MQ=255)
                                                     aaTTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATg                                                                                                  <  2:1123693/150‑1 (MQ=255)
                                                         tACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAggg                                                                                              >  2:3603411/1‑150 (MQ=255)
                                                            cGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGgcag                                                                                           >  2:2439266/1‑150 (MQ=255)
                                                            cGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGgcag                                                                                           <  1:4533394/150‑1 (MQ=255)
                                                              cacaGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGgcagca                                                                                         >  2:4563596/1‑150 (MQ=255)
                                                               acaGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCAt                                                                                        <  2:4092148/150‑1 (MQ=255)
                                                                caGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATg                                                                                                  <  2:2651575/139‑1 (MQ=255)
                                                                 aGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATgg                                                                                      >  1:803581/1‑150 (MQ=255)
                                                                   tttACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGaa                                                                                    <  2:775236/150‑1 (MQ=255)
                                                                    ttACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGaaa                                                                                   >  2:3932271/1‑150 (MQ=255)
                                                                          cGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAg                                                                             <  1:4312479/150‑1 (MQ=255)
                                                                          cGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAg                                                                             >  1:2026184/1‑150 (MQ=255)
                                                                            aaGCGGCATATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGAt                                                                           <  2:4379857/150‑1 (MQ=255)
                                                                            aaGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGAt                                                                           <  2:1930749/150‑1 (MQ=255)
                                                                               cGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTg                                                                        >  1:227108/1‑150 (MQ=255)
                                                                                  cAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGAtt                                                                     <  1:2644676/150‑1 (MQ=255)
                                                                                    gATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATtgt                                                                   >  2:3166200/1‑150 (MQ=255)
                                                                                      tAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGa                                                                 <  1:4555911/150‑1 (MQ=255)
                                                                                       aaaCGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGAt                                                                <  2:3794754/150‑1 (MQ=255)
                                                                                        aaCGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATa                                                               >  1:3630192/1‑150 (MQ=255)
                                                                                        aaCGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATa                                                               >  2:214330/1‑150 (MQ=255)
                                                                                               tAATGTTATACATATCACTCTAAAATGTTTGTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGAGAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCACAGCTCTGAGTGTGATAGGGggcg                                                        <  2:2190266/150‑1 (MQ=255)
                                                                                                   gTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCAt                                                    >  1:921068/1‑150 (MQ=255)
                                                                                                    ttATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATc                                                   <  2:803581/150‑1 (MQ=255)
                                                                                                    ttATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATc                                                   >  2:1985086/1‑150 (MQ=255)
                                                                                                     tataCATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCa                                                  >  1:4651957/1‑150 (MQ=255)
                                                                                                       taCATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAAt                                                <  1:1404259/150‑1 (MQ=255)
                                                                                                        aCATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCCAAGATCTGATTGTGATAGGGGGCGGCATCAATg                                               >  2:263470/1‑150 (MQ=255)
                                                                                                            atCACTCTAAAATGTTAATTCAATGTTACCTAAAGCGCGAATCTTTGCTAAAATGATCGATAACGAACATATATTAGCTTTAACGAAAGAGAATGAGTGCACCATGGACACCAAATATCTGATACTGATAGCGGGCGGCATCAAAGCTGc                                           >  2:1795253/1‑150 (MQ=255)
                                                                                                             tCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCt                                          <  1:2068945/150‑1 (MQ=255)
                                                                                                             tCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCt                                          <  1:1480223/150‑1 (MQ=255)
                                                                                                             tCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCt                                          >  2:4781179/1‑150 (MQ=255)
                                                                                                             tCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCt                                          >  2:125951/1‑150 (MQ=255)
                                                                                                              cACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTg                                         >  1:1498714/1‑150 (MQ=255)
                                                                                                                    aaaaTGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCACAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATcg                                   >  2:3481792/1‑150 (MQ=255)
                                                                                                                      aaTGTTTTTTCAATGTTACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATcgcg                                 >  2:2593074/1‑150 (MQ=255)
                                                                                                                       aTGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCgg                                >  1:2576537/1‑150 (MQ=255)
                                                                                                                        tGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGc                               <  2:644440/150‑1 (MQ=255)
                                                                                                                        tGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGc                               >  2:3602782/1‑150 (MQ=255)
                                                                                                                        tGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGc                               >  1:2803132/1‑150 (MQ=255)
                                                                                                                         gTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCa                              >  1:2304698/1‑150 (MQ=255)
                                                                                                                           tttttCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGa                            >  1:1213990/1‑150 (MQ=255)
                                                                                                                             tttCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGAcg                          >  1:4023276/1‑150 (MQ=255)
                                                                                                                              ttCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGAcgc                         >  2:481784/1‑150 (MQ=255)
                                                                                                                               tCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGAcgcc                        >  1:2944677/1‑150 (MQ=255)
                                                                                                                                cAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGAcgccg                       >  1:4751950/1‑150 (MQ=255)
                                                                                                                                  aTGTTACCTAAAGCGCGATCCTATGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACTAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCt                     >  1:3338904/1‑150 (MQ=255)
                                                                                                                                   tGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGACTGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTg                    >  2:3858396/1‑150 (MQ=255)
                                                                                                                                   tGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTg                    <  1:3602782/150‑1 (MQ=255)
                                                                                                                                    gTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTgg                   <  1:1211270/150‑1 (MQ=255)
                                                                                                                                    gTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTgg                   <  2:872171/150‑1 (MQ=255)
                                                                                                                                     ttACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGa                  <  1:2699027/150‑1 (MQ=255)
                                                                                                                                     ttACCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGa                  >  1:1807995/1‑150 (MQ=255)
                                                                                                                                      tACCTAACGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGAc                 <  1:237206/150‑1 (MQ=255)
                                                                                                                                      tACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGAc                 >  2:1719136/1‑150 (MQ=255)
                                                                                                                                       aCCTAAAGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGAcg                >  1:3062085/1‑150 (MQ=255)
                                                                                                                                        ccTAAAGCGCGATTCTTTGCTAATATGTTCGGTAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGAcgc               >  1:390897/1‑150 (MQ=255)
                                                                                                                                           aaaGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGt            >  1:3504689/1‑150 (MQ=255)
                                                                                                                                            aaGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGtt           <  2:333933/150‑1 (MQ=255)
                                                                                                                                             aGCGCGATCCTTTGCTAATATGCTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGttt          <  2:3092078/150‑1 (MQ=255)
                                                                                                                                               cgcgATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGTTTAt        <  2:3676464/150‑1 (MQ=255)
                                                                                                                                                 cgATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGTTTATcc      <  2:392115/150‑1 (MQ=255)
                                                                                                                                                  gATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGTTTATCCg     >  2:1654273/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGTTTATCCGTGC  >  CP009273/3555162‑3555460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A