Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I1 R1 274 511.2 22276341 99.2% 22098130 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,673,519 N→C intergenic (+68/+30) fabI → / ← PA14_RS16690 enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,673,5190NC99.8% 1529.8 / NA 422intergenic (+68/+30)fabI/PA14_RS16690enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base C (224/197);  minor base G (1/0);  total (225/197)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 430 total aligned reads displayed.

CCAGCGGTATCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGNGTAAACGGCCTTTTTCTTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAG  >  NC_008463/3673372‑3673667
                                                                                                                                                   |                                                                                                                                                    
ccAGCGGTATCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGt                                                                                                                                                    <  1:69377/150‑1 (MQ=255)
 cAGCGGTCTCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTGTCATGGGCGGAATAAAGAAAAGGCCGCGTa                                                                                                                                                   >  2:10264077/1‑150 (MQ=255)
  aGCGGTATCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTaa                                                                                                                                                  >  1:8466646/1‑150 (MQ=255)
  aGCGGTATCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTaa                                                                                                                                                  <  2:2426370/150‑1 (MQ=255)
     ggTATCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCg                                                                                                                                               >  1:7276994/1‑150 (MQ=255)
      gTATCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCgg                                                                                                                                              <  2:9160255/150‑1 (MQ=255)
      gTATCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCgg                                                                                                                                              <  1:1864196/150‑1 (MQ=255)
        aTCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGcc                                                                                                                                            <  1:6865079/150‑1 (MQ=255)
           aGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCttt                                                                                                                                         >  1:1649865/1‑150 (MQ=255)
           aGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCttt                                                                                                                                         <  2:8361488/150‑1 (MQ=255)
            ggggCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACAGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTGTGCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCgttt                                                                                                                                        >  1:8212331/3‑150 (MQ=255)
             tGGCGTGATCCTCTACGTCGATGGCGGCTTCAACACTCCCGCCATGGGCCCGCTGTACGACGAATGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCATTTTTCTTGGGCGGAATAACCAAAAGGCCGCGTAAGCGGCCttttc                                                                                                                                       <  2:5146551/150‑2 (MQ=255)
             tGGCGAGATCCTCTACGTCGATGGCGGCTACACCACTACCGCCAGGGGCCCGCTGGACGACGACTGATCTCCCCGCTCAGGCAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATCAAGAAACGGCCGCGTAAGCGGCCttttc                                                                                                                                       <  2:10005149/150‑2 (MQ=255)
              ggCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCttttct                                                                                                                                      >  2:10933488/1‑148 (MQ=255)
                  agaTCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCttttctctgc                                                                                                                                  >  1:6358416/1‑144 (MQ=255)
                       ctctACGTCGATGGCGGCTACAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAACAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCAtctc                                                                                                                             <  1:2645894/150‑1 (MQ=255)
                        tctACGTCGAGGGCGGCTTCAACACTCCCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTACATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCg                                                                                                                            <  1:2176502/150‑1 (MQ=255)
                            cGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGtct                                                                                                                        >  1:1095952/1‑150 (MQ=255)
                             gTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGtctc                                                                                                                       <  2:6465797/150‑1 (MQ=255)
                              tCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCa                                                                                                                      <  1:10042317/150‑1 (MQ=255)
                              tCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCa                                                                                                                      >  1:689871/1‑150 (MQ=255)
                              tCGATGGCGGCTTCAACACTACCGCCAGGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCa                                                                                                                      >  2:4900291/1‑150 (MQ=255)
                                    gcggcTTTAACACTCCCGCCAGGGGCCCGCTGGACGACGACTGATCACCCCGCACATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGTAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTAttt                                                                                                                <  1:8706419/150‑1 (MQ=255)
                                    gcggcTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTAttt                                                                                                                <  1:7979207/150‑1 (MQ=255)
                                    gcggcTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTAttt                                                                                                                <  2:5995032/150‑1 (MQ=255)
                                      ggcTTCAACACTACCGCCAGGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAAAAAACAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTtct                                                                                                              <  1:2286408/150‑1 (MQ=255)
                                       gcTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCATCGAGCGGCCTTTTTCATGGGCGGAATAAAGATAAGGCCGCGTAAGCGGCCTTTGCTCTGCATCTCGGTCTCAGTATTtcac                                                                                                             >  2:8472487/1‑148 (MQ=255)
                                         ttCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGa                                                                                                           <  1:7400985/150‑1 (MQ=255)
                                         tacAACACTACCGCCATGGGCCCGCTGGACGCCGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCTTGGGCGGAGTAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGa                                                                                                           <  1:5963942/148‑1 (MQ=255)
                                           cAACCCGACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCCTGAAAAAGGGCCGCTTCGAGCGGCCTTTTTCTTGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACt                                                                                                         <  1:9468636/150‑1 (MQ=255)
                                            aaCACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACtt                                                                                                        <  2:8538165/150‑1 (MQ=255)
                                            aaCACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACtt                                                                                                        >  1:3216560/1‑150 (MQ=255)
                                             acacTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTc                                                                                                       >  1:5858665/1‑150 (MQ=255)
                                                cTACCGCCAGGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGc                                                                                                    <  1:7857724/150‑1 (MQ=255)
                                                 tACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGcc                                                                                                   >  2:741368/1‑150 (MQ=255)
                                                  cccGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCACATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCTTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCt                                                                                                  <  1:3648537/149‑1 (MQ=255)
                                                     gCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGt                                                                                               >  1:8362809/1‑150 (MQ=255)
                                                      ccATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTc                                                                                              >  1:9687774/1‑150 (MQ=255)
                                                         tGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTACGAGCGGCCTTTTTCATGGGCGGAATAACGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTg                                                                                           <  1:9596314/150‑1 (MQ=255)
                                                          gggCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGc                                                                                          <  2:10385448/150‑1 (MQ=255)
                                                             cccGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAAc                                                                                       <  1:863629/150‑1 (MQ=255)
                                                             cccGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGGCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAAc                                                                                       >  2:9395579/1‑150 (MQ=255)
                                                                gCTGGACGACGACTGATCACCCCGCTCACGAAAAATGGCCGCTTCGAGCGGCCTTTATCATGGGCGGAATAAAGCAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGa                                                                                    <  2:872095/150‑1 (MQ=255)
                                                                  tggtctacgacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCATTTTTCATGGGCGGAATAAAGAAACGGCCGCGTATGCGGCCTTTTCTCTGCATCTCGGTATCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAcg                                                                                  <  1:10172205/144‑1 (MQ=255)
                                                                   ggACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAcgg                                                                                 >  2:892654/1‑150 (MQ=255)
                                                                   ggACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAcgg                                                                                 >  2:216353/1‑150 (MQ=255)
                                                                    gacgacgacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTGGTCTCTGCATCTCGGTCTCAGTCTTTCTCGACTTCCGCCTTGTCCTGCAACTGAcggc                                                                                >  2:6082814/1‑150 (MQ=255)
                                                                         cgacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAACAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAg                                                                           <  1:5424577/150‑1 (MQ=255)
                                                                         cgacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAg                                                                           >  1:3483663/1‑150 (MQ=255)
                                                                           acTGATCACCCCGCTCATGAAAAAGGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAggc                                                                         <  2:5459238/150‑1 (MQ=255)
                                                                               aTCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGgcggcg                                                                     >  1:10534399/1‑150 (MQ=255)
                                                                                tCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGa                                                                    >  2:1078496/1‑150 (MQ=255)
                                                                                  aCCCCGCTCATGACAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAg                                                                  <  2:5705362/150‑1 (MQ=255)
                                                                                  aCCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAg                                                                  <  1:649717/150‑1 (MQ=255)
                                                                                    cccGCTCTTGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTc                                                                <  1:5278313/150‑1 (MQ=255)
                                                                                     ccGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCt                                                               >  2:4238649/1‑150 (MQ=255)
                                                                                      cGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTg                                                              >  1:8804591/1‑150 (MQ=255)
                                                                                        cTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGcc                                                            <  1:7713592/150‑1 (MQ=255)
                                                                                          cATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTg                                                          >  2:7909684/1‑150 (MQ=255)
                                                                                            tGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTc                                                        <  1:1378976/150‑1 (MQ=255)
                                                                                             gAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCGGTcc                                                       >  2:3057409/1‑150 (MQ=255)
                                                                                                aaaTGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCt                                                    >  2:2884907/1‑150 (MQ=255)
                                                                                                 aaTGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTg                                                   >  2:1367792/1‑150 (MQ=255)
                                                                                                 aaTGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTg                                                   >  1:3386061/1‑150 (MQ=255)
                                                                                                  aTGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTgc                                                  <  2:2075713/150‑1 (MQ=255)
                                                                                                   tGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTgcg                                                 >  2:632480/1‑150 (MQ=255)
                                                                                                   tGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTgcg                                                 <  1:11128860/150‑1 (MQ=255)
                                                                                                       cGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGaa                                             >  1:2954408/1‑150 (MQ=255)
                                                                                                       cGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGaa                                             <  2:9721146/150‑1 (MQ=255)
                                                                                                       cGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTGTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTGGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGaa                                             <  2:6869667/150‑1 (MQ=255)
                                                                                                        gCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAg                                            >  2:1159291/1‑150 (MQ=255)
                                                                                                          ttCGAGCGGCCTTTTTCATGGGCGGAATAAAGAACAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATGTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGcc                                          <  2:5045459/150‑1 (MQ=255)
                                                                                                           tCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCa                                         >  2:6955586/1‑150 (MQ=255)
                                                                                                            cGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAg                                        <  2:7216544/150‑1 (MQ=255)
                                                                                                              aGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGa                                      >  1:2545625/1‑150 (MQ=255)
                                                                                                              aGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGa                                      >  1:4504455/1‑150 (MQ=255)
                                                                                                               gCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGaa                                     <  2:8094321/150‑1 (MQ=255)
                                                                                                               gCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGACTTTACTCTGCATCTCGGTCTCATTATTTCTCGACTTCCGCCTAGTCATTCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGaa                                     >  2:4017041/1‑150 (MQ=255)
                                                                                                                cGGCCTTTTTCATGGGCGGAATAAAGAACAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAg                                    <  1:6667229/150‑1 (MQ=255)
                                                                                                                cGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGacg                                    >  1:9221619/1‑148 (MQ=255)
                                                                                                                 ggCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTACGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgc                                   >  2:4479084/1‑150 (MQ=255)
                                                                                                                       tttCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGt                             <  2:8826545/150‑1 (MQ=255)
                                                                                                                         tCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTAc                           >  1:4810029/1‑150 (MQ=255)
                                                                                                                          cATGGGCGGAATAAAGAACAGGCCGCGTACGCGGCCTTTACTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACGGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACa                          <  1:4399577/150‑1 (MQ=255)
                                                                                                                            tGGGCGGAATAAAGAAAAGGCCGCGTACGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATg                        <  1:5471831/150‑1 (MQ=255)
                                                                                                                               gCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGcc                     >  2:2791536/1‑150 (MQ=255)
                                                                                                                                cGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCt                    >  1:3965051/1‑150 (MQ=255)
                                                                                                                                cGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGACCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCt                    >  1:4113995/1‑150 (MQ=255)
                                                                                                                                 ggAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCATGCAACTGACGGCGGAAGGCGGCGAAGTCTGACTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCtt                   >  2:6598227/1‑150 (MQ=255)
                                                                                                                                     tAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcg               >  2:10574813/1‑150 (MQ=255)
                                                                                                                                      aaaGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgt              >  1:10832665/1‑150 (MQ=255)
                                                                                                                                      aaaGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgt              >  2:3763829/1‑150 (MQ=255)
                                                                                                                                        aGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcg            >  1:5029899/1‑150 (MQ=255)
                                                                                                                                         gAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCCTCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGGCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcgt           <  2:7593538/150‑1 (MQ=255)
                                                                                                                                          aaaaGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcgtc          >  2:3517696/1‑150 (MQ=255)
                                                                                                                                           aaaGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcgtcg         >  2:33660/1‑150 (MQ=255)
                                                                                                                                               gCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGgaga     >  2:1636033/1‑150 (MQ=255)
                                                                                                                                               gCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGgaga     <  2:2364364/150‑1 (MQ=255)
                                                                                                                                                 cgcgTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATa   >  1:76620/1‑150 (MQ=255)
                                                                                                                                                  gCGTAAGCGGCCTTTTCTCGGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAg  >  2:2618460/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCAGCGGTATCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGNGTAAACGGCCTTTTTCTTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAG  >  NC_008463/3673372‑3673667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A