Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	255	512.9	22377240	99.2%	22198222	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,028,201	2 bp→CG	intergenic (‑123/+13)	PA14_RS22975 ← / ← PA14_RS22980	Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,028,201	0	G	C	98.8%	1631.2 / 5.7	426	intergenic (‑123/+14)	PA14_RS22975/PA14_RS22980	Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein
Reads supporting (aligned to +/- strand): ref base G (5/0); new base C (207/214); total (212/214)
Fisher's exact test for biased strand distribution p-value = 2.98e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01
*	NC_008463	5,028,202	0	T	G	98.8%	1656.9 / 6.6	426	intergenic (‑124/+13)	PA14_RS22975/PA14_RS22980	Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (207/214); minor base . (5/0); total (212/214)
Fisher's exact test for biased strand distribution p-value = 2.98e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 426 total aligned reads displayed.

CGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGGTGGGTTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGTCC  >  NC_008463/5028060‑5028338

||

 gTCGGCCGGCACCGCCCCGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGG‑GGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTtccctcc                                                                                                                                <  2:5229681/150‑1 (MQ=255)

 gTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTtccctcc                                                                                                                                <  1:4718193/150‑1 (MQ=255)

  tCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTtccctccc                                                                                                                               <  2:8073129/150‑1 (MQ=255)

     gccggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGACCGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTccctcccctc                                                                                                                            <  2:5563527/150‑1 (MQ=255)

      ccggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCa                                                                                                                           >  2:3768520/1‑150 (MQ=255)

       cggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAg                                                                                                                          <  2:7337607/150‑1 (MQ=255)

        ggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAgg                                                                                                                         >  1:1985226/1‑150 (MQ=255)

        ggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAgg                                                                                                                         >  2:4144883/1‑150 (MQ=255)

          cACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGcc                                                                                                                       <  2:6720237/150‑1 (MQ=255)

           aCCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCt                                                                                                                      >  1:4927883/1‑150 (MQ=255)

            ccGCCACGCTGTGGCTGTCCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTg                                                                                                                     <  2:1823949/150‑1 (MQ=255)

                cACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGgcg                                                                                                                 >  1:6810384/1‑150 (MQ=255)

                cACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGgcg                                                                                                                 <  1:6002369/150‑1 (MQ=255)

                   gCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGgcgcgc                                                                                                              <  1:547416/150‑1 (MQ=255)

                    cTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGACGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCt                                                                                                             <  2:3982702/150‑1 (MQ=255)

                    cTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCt                                                                                                             <  1:5544641/150‑1 (MQ=255)

                          cTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGa                                                                                                       >  2:4671644/1‑150 (MQ=255)

                          cTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGa                                                                                                       <  2:6994993/150‑1 (MQ=255)

                           tGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCGCAGGCCTGGGCGCGCTGGCCGAc                                                                                                      >  1:2927786/1‑150 (MQ=255)

                             aCCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCa                                                                                                    <  1:76100/150‑1 (MQ=255)

                               cgccgAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAgg                                                                                                  >  2:4477771/1‑150 (MQ=255)

                                gccgAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGAGCAGGc                                                                                                 >  2:2950832/1‑150 (MQ=255)

                                   gAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCa                                                                                              <  1:10202693/150‑1 (MQ=255)

                                   gAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCa                                                                                              <  1:3614067/150‑1 (MQ=255)

                                       gACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTc                                                                                          <  1:3131075/150‑1 (MQ=255)

                                          aGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTGCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGc                                                                                       <  2:1611869/150‑1 (MQ=255)

                                           gAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCg                                                                                      >  1:332743/1‑150 (MQ=255)

                                             aCGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGAc                                                                                    >  2:10699542/1‑150 (MQ=255)

                                                  gcgGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTtct                                                                               <  2:2308905/150‑1 (MQ=255)

                                                   cgGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTtctc                                                                              <  1:3424514/150‑1 (MQ=255)

                                                    ggTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTcg                                                                             <  2:6838963/150‑1 (MQ=255)

                                                      gtggccGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTcgc                                                                            >  2:10057627/5‑150 (MQ=255)

                                                         gccGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGcgcc                                                                         <  1:10504966/149‑1 (MQ=255)

                                                          ccGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGcgccg                                                                        >  2:6949479/1‑150 (MQ=255)

                                                            gtggtgGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGAGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCt                                                                      >  1:1304704/1‑150 (MQ=255)

                                                             tggtggTCAGGGAGTATTTCACGAGTCGACGCCGGATCGGGGAGGAAGTCCGACCAGCCTAAACGTATCACACCGGACGCG‑TCATCCCTCCCCACACGACTCGGCGCGCAGGACGACCAGGCTCAGCACGCCGACCATCTCGCGACGCTg                                                                     >  2:3018904/1‑150 (MQ=255)

                                                              ggtggGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTgc                                                                    >  1:9461371/1‑150 (MQ=255)

                                                               gtggGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTgcg                                                                   >  1:10796407/1‑150 (MQ=255)

                                                               gtggGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTgcg                                                                   <  1:9359632/150‑1 (MQ=255)

                                                                 gggCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGcc                                                                 >  1:746705/1‑150 (MQ=255)

                                                                     aGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGt                                                             <  1:2243486/150‑1 (MQ=255)

                                                                      gggAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTa                                                            >  1:5198018/1‑150 (MQ=255)

                                                                      gggAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTa                                                            >  1:9044471/1‑150 (MQ=255)

                                                                        gAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGc                                                          <  2:1302876/150‑1 (MQ=255)

                                                                          gTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCgg                                                        >  2:3152060/1‑150 (MQ=255)

                                                                          gTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCgg                                                        >  1:11065416/1‑150 (MQ=255)

                                                                              ttCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGc                                                    >  1:7977270/1‑150 (MQ=255)

                                                                              ttCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGc                                                    >  2:2020075/1‑150 (MQ=255)

                                                                               tCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCa                                                   <  2:4986982/150‑1 (MQ=255)

                                                                                cACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCaa                                                  <  1:9757407/150‑1 (MQ=255)

                                                                                cACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCATCTCGCGCCGCTGCGCCAGGTAGCGGGTGCaa                                                  >  1:10416526/1‑150 (MQ=255)

                                                                                 aCGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAAc                                                 >  1:8652117/1‑150 (MQ=255)

                                                                                  cGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCACCt                                                >  2:7764650/1‑150 (MQ=255)

                                                                                  cGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACt                                                <  1:7699985/150‑1 (MQ=255)

                                                                                  cGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACt                                                >  2:575285/1‑150 (MQ=255)

                                                                                    aGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGa                                              >  2:310503/1‑150 (MQ=255)

                                                                                    aGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTGCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGa                                              >  2:6271669/1‑150 (MQ=255)

                                                                                    aGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCGGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGa                                              >  1:1418625/1‑150 (MQ=255)

                                                                                     ggCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGac                                             >  2:7573198/1‑150 (MQ=255)

                                                                                     ggCGACGCCGGATCGGGGAGGAAGTGAGACCAGCCTAAACGTATCACCCC‑GGTCGCGTATTCCCTCCACTCAGGCCTGGGCGCGCTGGCCGACCAGGCTAAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGAAGCGGGTGCAACAGac                                             >  1:8820672/1‑150 (MQ=255)

                                                                                         aCGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGGCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGc                                         >  2:5919475/1‑150 (MQ=255)

                                                                                           gCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCag                                       <  1:6454917/150‑1 (MQ=255)

                                                                                           gCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCag                                       <  2:2648726/150‑1 (MQ=255)

                                                                                            ccGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCagg                                      >  2:11076797/1‑150 (MQ=255)

                                                                                            ccGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCagg                                      <  1:2111022/150‑1 (MQ=255)

                                                                                            ccGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCagg                                      >  1:616730/1‑150 (MQ=255)

                                                                                              ggATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggca                                    >  2:5123700/1‑148 (MQ=255)

                                                                                              ggATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaa                                    <  1:2703624/150‑1 (MQ=255)

                                                                                               gATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaag                                   <  1:10008568/150‑1 (MQ=255)

                                                                                               gATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaag                                   >  1:7108484/1‑150 (MQ=255)

                                                                                                 tCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaagga                                 <  2:8643673/150‑1 (MQ=255)

                                                                                                  cGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAaggag                                <  2:3290614/150‑1 (MQ=255)

                                                                                                  cGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAaggag                                <  1:9674954/150‑1 (MQ=255)

                                                                                                  cGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAaggag                                <  2:10892820/150‑1 (MQ=255)

                                                                                                   ggggAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAaggagg                               <  1:915411/150‑1 (MQ=255)

                                                                                                   ggggAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAaggagg                               <  2:11181154/150‑1 (MQ=255)

                                                                                                    gggAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGc                              <  1:2373941/150‑1 (MQ=255)

                                                                                                      gaggaAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGa                            <  1:8299897/150‑1 (MQ=255)

                                                                                                         gaAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGt                         <  1:1673780/150‑1 (MQ=255)

                                                                                                           aGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTg                       <  1:8450748/150‑1 (MQ=255)

                                                                                                            gTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGa                      >  2:3503949/1‑150 (MQ=255)

                                                                                                              gCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGc                    >  1:6381138/1‑150 (MQ=255)

                                                                                                               cGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCa                   <  2:2500499/150‑1 (MQ=255)

                                                                                                                 aCCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCAcc                 >  2:4872080/1‑150 (MQ=255)

                                                                                                                  ccAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCCGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCACCTGACACGCAGGCAGGAGGCGAAGTTGAGCACCt                <  2:1056497/150‑1 (MQ=255)

                                                                                                                   cAGCCTACACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTc               <  2:8603615/150‑1 (MQ=255)

                                                                                                                   cAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTc               <  1:4061612/150‑1 (MQ=255)

                                                                                                                     gCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgc             <  2:2171192/150‑1 (MQ=255)

                                                                                                                      ccTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgcc            <  1:5757190/150‑1 (MQ=255)

                                                                                                                      ccTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgcc            <  2:9577609/150‑1 (MQ=255)

                                                                                                                       cTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGACGTTGAGCACCTcgccg           >  2:9786117/1‑150 (MQ=255)

                                                                                                                        tAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCACCTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgccgc          >  2:9125761/1‑150 (MQ=255)

                                                                                                                           aCGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGAt       >  1:9573630/1‑150 (MQ=255)

                                                                                                                            cGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATa      >  1:2591379/1‑150 (MQ=255)

                                                                                                                              tttCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGTGCACCTCGCCGCGTTAGt    >  2:10249800/1‑150 (MQ=255)

                                                                                                                              tttCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGt    >  2:5350339/1‑150 (MQ=255)

                                                                                                                              tttCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGt    >  1:435178/1‑150 (MQ=255)

                                                                                                                              tttCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGt    <  1:3939539/150‑1 (MQ=255)

                                                                                                                                tCACCCCGGTCGCG‑TCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGCTAGTcc  >  2:7486172/1‑150 (MQ=255)

                                                                                                                                tCACCCCGGTCGCG‑TCTTCCCTCCCCTCAGGCCGGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGTcc  >  2:8496792/1‑150 (MQ=255)

||

CGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGGTGGGTTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGTCC  >  NC_008463/5028060‑5028338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A