Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	255	512.9	22386308	99.2%	22207217	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	1,128,156	3 bp→GCG	intergenic (+17/‑42)	PA14_RS05280 → / → PA14_RS05285	DUF1302 domain‑containing protein/DUF1329 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	1,128,156	0	N	G	99.8%	1585.2 / NA	422	intergenic (+17/‑44)	PA14_RS05280/PA14_RS05285	DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
Reads supporting (aligned to +/- strand): ref base N (0/0); major base G (206/215); minor base T (1/0); total (207/215)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NC_008463	1,128,157	0	N	C	99.8%	1560.5 / NA	422	intergenic (+18/‑43)	PA14_RS05280/PA14_RS05285	DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
Reads supporting (aligned to +/- strand): ref base N (0/0); major base C (206/215); minor base G (1/0); total (207/215)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NC_008463	1,128,158	0	N	G	99.8%	1546.3 / NA	422	intergenic (+19/‑42)	PA14_RS05280/PA14_RS05285	DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
Reads supporting (aligned to +/- strand): ref base N (0/0); major base G (206/214); minor base T (0/1); total (207/215)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 422 total aligned reads displayed.

CCTGGGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGC  >  NC_008463/1128010‑1128304

|||

ccTGGGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGa                                                                                                                                                   >  1:1797095/1‑150 (MQ=255)

 cTGGGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCGGATGTACGCCGGCCCGGTGCGac                                                                                                                                                  >  1:4240865/1‑149 (MQ=255)

  tGGGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGTACTTCTGATGTACGCCGGCCCGGTGCGAAc                                                                                                                                                 >  1:8024676/1‑150 (MQ=255)

  tGGGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACGTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAAc                                                                                                                                                 >  1:7673681/1‑150 (MQ=255)

   gggggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAAcc                                                                                                                                                <  2:10124519/150‑2 (MQ=255)

      ggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTACTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCggg                                                                                                                                             <  2:170838/150‑2 (MQ=255)

          gACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCTACGGCAAGTACACCCCCGTGGACGAGCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCgg                                                                                                                                         >  2:10330073/1‑150 (MQ=255)

           aCGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGc                                                                                                                                        <  1:10990190/150‑1 (MQ=255)

            cgcgGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCCTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCg                                                                                                                                       >  1:9098833/1‑150 (MQ=255)

            cgcgGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCg                                                                                                                                       >  2:2507847/1‑150 (MQ=255)

             gcgGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTAGGCATGAACTTCTGATGTAAGCCGGCCCGGTGCGAACCGGGCCGGCGa                                                                                                                                      >  1:3734178/1‑150 (MQ=255)

              cgGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAg                                                                                                                                     <  1:3580394/150‑1 (MQ=255)

              cgGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAg                                                                                                                                     <  2:5261095/150‑1 (MQ=255)

               ggAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGc                                                                                                                                    >  2:4267244/1‑150 (MQ=255)

               ggAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGATCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGc                                                                                                                                    <  2:2285581/150‑1 (MQ=255)

                gAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTTCGCCGGCCCGGTGCGAACCGGGCCGGCGAGCa                                                                                                                                   >  1:9238717/1‑150 (MQ=255)

                 aGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAg                                                                                                                                  <  2:9239917/150‑1 (MQ=255)

                  gtcccAGAACACCTACACCGCGAGCCTCTCCTACACCCACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTAGCGCTCAGCTTCGGCATGACCGTCGGATGGTAGCCGGGCCGGTGCGAACCGGGCCGGCGAGCCgg                                                                                                                                 >  1:5104778/4‑150 (MQ=255)

                  gTACCAGAACACCTACCCCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAgg                                                                                                                                 <  2:828655/150‑1 (MQ=255)

                    aCCAGAACACCTACACCGCGAGCCTCTCCTACACCACCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGAc                                                                                                                               <  1:6510383/150‑1 (MQ=255)

                    aCCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCCGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGAc                                                                                                                               >  2:411030/1‑150 (MQ=255)

                     ccAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACt                                                                                                                              >  2:364199/1‑150 (MQ=255)

                     ccAGAACACCAACCCCGCGAGCCTCTCCTACACCAACTTCTTCGACGGAAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGATCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACt                                                                                                                              <  1:307773/150‑1 (MQ=255)

                       aGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTa                                                                                                                            >  2:787744/1‑150 (MQ=255)

                       aGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACATCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCCGTGCGAACCCGGCCGGCGAGCCGGACttt                                                                                                                            >  1:9328927/1‑149 (MQ=255)

                           cacCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGa                                                                                                                        >  2:4512666/1‑150 (MQ=255)

                            acCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACCCCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAg                                                                                                                       <  1:1569417/150‑1 (MQ=255)

                             ccTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAgg                                                                                                                      <  2:9032783/150‑1 (MQ=255)

                             ccTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAgg                                                                                                                      <  1:8361671/150‑1 (MQ=255)

                              cTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGAGCGCGACTTCGTCGCGCTCAGCTTCGGCCTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGGCGGCGAGCAGGACTTAACGAGga                                                                                                                     >  1:5752530/1‑150 (MQ=255)

                              cTACACCGCGAGCCTCTCCTACACCAACTCCTTCGTCGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGTTGTACGCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTAACGAGga                                                                                                                     <  1:379412/150‑1 (MQ=255)

                               tACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgac                                                                                                                    <  2:10981685/150‑1 (MQ=255)

                                   ccGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCCGGACTTAAAGAGGacgacg                                                                                                                >  1:1302050/1‑150 (MQ=255)

                                    cgcgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGGGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGAcgacga                                                                                                               <  2:5850150/150‑1 (MQ=255)

                                     gcgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAg                                                                                                              >  2:7197598/1‑150 (MQ=255)

                                     gcgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAg                                                                                                              <  1:3102986/150‑1 (MQ=255)

                                      cgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGc                                                                                                             <  1:6337870/150‑1 (MQ=255)

                                         gCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACATATGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGaa                                                                                                          >  1:6984160/1‑150 (MQ=255)

                                          ccTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAAt                                                                                                         <  1:9584945/150‑1 (MQ=255)

                                            tctcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGa                                                                                                       >  1:10014497/1‑150 (MQ=255)

                                             ctcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGaa                                                                                                      <  1:3216647/150‑1 (MQ=255)

                                              tcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGaaa                                                                                                     <  1:5472520/150‑1 (MQ=255)

                                              tcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGAGCGCGACATCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCCGCCCGGTGCGAACCGGGCCGGCGCGCAGGACTTAACGAGGACGACGAGCGAATGaaa                                                                                                     >  1:3281621/1‑150 (MQ=255)

                                                 tACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaaca                                                                                                  <  2:10088275/150‑1 (MQ=255)

                                                   cacCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaacaac                                                                                                <  2:3670257/150‑1 (MQ=255)

                                                     ccAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGa                                                                                              <  2:1537212/150‑1 (MQ=255)

                                                      cAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTTCGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGTCGACGAGCGAATGAAAACAACGaa                                                                                             >  1:10850448/1‑150 (MQ=255)

                                                       aaCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGGCGCGCTCAGCGTCGGCCTGAACGTAGGATGTTCGCCGGCCCGGTGCGGACCGGGCCGGCGGGCAGGACCTAAAGAGGACGACGCGCGAATGGAAACAACggat                                                                                            >  1:7520548/1‑147 (MQ=255)

                                                        aCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGa                                                                                           <  2:3338432/150‑1 (MQ=255)

                                                            cttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAttct                                                                                       <  1:7240034/150‑1 (MQ=255)

                                                            cttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAttct                                                                                       <  1:4110930/150‑1 (MQ=255)

                                                              tcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAttcttc                                                                                     <  2:5703359/150‑1 (MQ=255)

                                                              tcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAttcttc                                                                                     <  1:1226920/150‑1 (MQ=255)

                                                              tcGACGGCAAGTACACCACCGTGGACGATCGCGACATCGTCGCGCACAGCTTAGGCATTAACTTCTGAATTACCCCGGACCCGTTCGAAACCGGCCGGCGTGCCGGACATAACGAGGACGACGAGCGAATGAAAACAAAGAAAAtaatta                                                                                     >  2:140742/1‑145 (MQ=255)

                                                               cGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATtcttct                                                                                    <  2:7392477/150‑1 (MQ=255)

                                                               cGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTATGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATtcttct                                                                                    <  2:13569/150‑1 (MQ=255)

                                                                gACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCGTCGGCATGAACTTCTGATGTTCGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTg                                                                                   >  2:9529320/1‑150 (MQ=255)

                                                                   ggCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCAt                                                                                <  2:102933/150‑1 (MQ=255)

                                                                   ggCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCAt                                                                                <  1:3410859/150‑1 (MQ=255)

                                                                       aGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGCGCAGGACTTAACGAGGACGACGAGCGAATGCAAACAACGAAGATTCTTCTGCATACCg                                                                            >  2:7766882/1‑150 (MQ=255)

                                                                         tACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGgt                                                                          >  2:7050424/1‑150 (MQ=255)

                                                                              caccGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGTCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCTAATGAAAACAACGAAGATTCTTCTGCTTACCGGTGTACt                                                                     <  2:9716506/150‑1 (MQ=255)

                                                                              caccGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACt                                                                     <  2:8788828/150‑1 (MQ=255)

                                                                              caccGTGGACGATCGCGACTTAGTCGCGCTCAGCTTAGGCATGAAATTCAGATGTACGCCGGCCCGGTGCGAAACGGGCCGGCGAGCCGGACTTAACGAGGACTACGAGCGAATGAAAACAACGAAGATTAATCTGCATAACGGTGTACt                                                                     >  2:1156788/1‑150 (MQ=255)

                                                                               accGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCAAGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTg                                                                    >  2:11112240/1‑150 (MQ=255)

                                                                               accGTGGACGATCGCGACATCGTCGCGCTCAGCTTCGGCATGAACATCTGATGTAAGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAAAGAAAACAACGAAGATTCTTATGCATACCGGTGTACTg                                                                    >  1:2674360/1‑150 (MQ=255)

                                                                                 cGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTATGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACATAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGc                                                                  >  2:6998177/1‑150 (MQ=255)

                                                                                         aTCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTACCGAGGACGACGAGCGAATGAAAACACCGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGcc                                                          <  1:6672514/150‑1 (MQ=255)

                                                                                            gcgACTACGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGCGCGAATGAAAACAACGCAGATTCTTCTGCATACCGGGGTACTGGCCCTGAGCctgc                                                       <  1:4618844/150‑1 (MQ=255)

                                                                                               aCTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTgg                                                    >  2:8577855/1‑150 (MQ=255)

                                                                                                   cgtcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCAc                                                >  2:4704301/1‑150 (MQ=255)

                                                                                                    gtcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCAcc                                               >  1:9825331/1‑150 (MQ=255)

                                                                                                     tcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGACTGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCAccc                                              >  2:5495612/1‑150 (MQ=255)

                                                                                                        cgcTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAgg                                           <  2:3878706/150‑1 (MQ=255)

                                                                                                         gcTCAGCTGCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGt                                          <  1:6867001/150‑1 (MQ=255)

                                                                                                          cTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTg                                         >  2:5016705/1‑150 (MQ=255)

                                                                                                            cAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGAt                                       >  1:10679178/1‑150 (MQ=255)

                                                                                                               cTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggc                                    <  1:8788106/150‑1 (MQ=255)

                                                                                                               cTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggc                                    <  1:10246361/150‑1 (MQ=255)

                                                                                                                ttCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcg                                   >  1:10168226/1‑150 (MQ=255)

                                                                                                                 tCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcgg                                  <  2:6119316/150‑1 (MQ=255)

                                                                                                                   ggCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGgcggcg                                >  2:2926386/1‑150 (MQ=255)

                                                                                                                      aTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTa                             >  1:10454544/1‑150 (MQ=255)

                                                                                                                      aTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTa                             >  2:6719656/1‑150 (MQ=255)

                                                                                                                        gAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATc                           >  1:485630/1‑150 (MQ=255)

                                                                                                                          aCTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccg                         <  2:10603364/150‑1 (MQ=255)

                                                                                                                          aCTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccg                         >  2:7598880/1‑150 (MQ=255)

                                                                                                                           cTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccgc                        >  2:6159749/1‑150 (MQ=255)

                                                                                                                            ttCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccgcc                       >  1:4457012/1‑150 (MQ=255)

                                                                                                                                gATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCcgacg                   >  2:2040704/1‑150 (MQ=255)

                                                                                                                                 aTGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCcgacga                  >  1:1930623/1‑150 (MQ=255)

                                                                                                                                  tGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCGTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAg                 >  2:6269039/1‑150 (MQ=255)

                                                                                                                                     aCGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggcg              >  2:5405279/1‑150 (MQ=255)

                                                                                                                                      cGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggcgg             >  2:1851951/1‑150 (MQ=255)

                                                                                                                                            cccGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGc       >  1:2161555/1‑150 (MQ=255)

                                                                                                                                             ccGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCt      >  1:8706895/1‑150 (MQ=255)

                                                                                                                                              cGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACAGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTc     >  2:2415421/1‑150 (MQ=255)

                                                                                                                                                gTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCgg   <  1:6137257/150‑1 (MQ=255)

                                                                                                                                                gTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCgg   >  1:8224734/1‑150 (MQ=255)

                                                                                                                                                 tGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGc  >  1:7788078/1‑150 (MQ=255)

|||

CCTGGGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGC  >  NC_008463/1128010‑1128304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A