Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I1 R1 256 475.0 21680010 99.2% 21506569 151.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 6,128,635 Δ1 bp intergenic (+100/‑108) PA14_RS28015 → / → gshA PaaI family thioesterase/glutamate‑‑cysteine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084636,128,6330G.98.5% 2180.0 / 26.3 458intergenic (+98/‑110)PA14_RS28015/gshAPaaI family thioesterase/glutamate‑‑cysteine ligase
Reads supporting (aligned to +/- strand):  ref base G (5/2);  new base . (180/269);  total (185/273)
Fisher's exact test for biased strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 472 total aligned reads displayed.

GGCAAGGCGACAAGCTAGTCGCGAAAGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACCGGGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAGCGATCTTCTCTCCCGCCGCCTGGCTCTGCTCG  >  NC_008463/6128483‑6128779
                                                                                                                                                      |                                                                                                                                                  
ggCAAGGCGACAAGCTAGTCGCGAAAGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACCg                                                                                                                                                    >  1:6353516/1‑151 (MQ=255)
ggCAAGGCGACAAGCTAGTCGCGAAAGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACCg                                                                                                                                                    <  1:5221235/151‑1 (MQ=255)
 gCAAGGCGACAAGCTAGTCGCGAAAGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACCgg                                                                                                                                                   >  1:7697885/1‑151 (MQ=255)
  cAAGGCGACAAGCTAGTCGCGAAAGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACCggc                                                                                                                                                  <  1:2350053/151‑2 (MQ=255)
    aGGCGACAAGCTAGTCGCGAAAGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACCGGccc                                                                                                                                                >  2:3880177/1‑151 (MQ=255)
        gACAAGCTAGTCGCGAAAGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAgg                                                                                                                                           <  1:1904546/151‑1 (MQ=255)
        gACAAGCTAGACGCGAAAGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGATTCGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAgg                                                                                                                                           <  1:8342941/151‑1 (MQ=255)
         aCAAGCTAGTCGCGAAAGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGACGGCGCGGCGACC‑GGCCCCAGGa                                                                                                                                          >  2:992167/1‑151 (MQ=255)
         aCAAGCTAGTCGCGAAAGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGa                                                                                                                                          <  1:4195598/151‑1 (MQ=255)
          cAAGCTAGTCGCGAAAGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGAc                                                                                                                                         >  1:3294298/1‑151 (MQ=255)
              cTAGTCGCGAAAGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAg                                                                                                                                     >  2:10437172/1‑151 (MQ=255)
               tAGTCGCGAAAGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGc                                                                                                                                    <  2:1701743/151‑1 (MQ=255)
                 gTCGCGAAAGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGa                                                                                                                                  >  1:4996892/1‑151 (MQ=255)
                  tCGCGAAAGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGaa                                                                                                                                 <  1:998571/151‑1 (MQ=255)
                    gcgAAAGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGaaaa                                                                                                                               >  1:9301186/1‑151 (MQ=255)
                       aaaGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGc                                                                                                                            >  1:1238637/1‑151 (MQ=255)
                        aaGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCa                                                                                                                           >  1:10829169/1‑151 (MQ=255)
                        aaGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCa                                                                                                                           >  1:10611006/1‑151 (MQ=255)
                        aaGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCa                                                                                                                           >  2:6242926/1‑151 (MQ=255)
                          ggACAACGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCAtt                                                                                                                         <  1:1493234/151‑1 (MQ=255)
                           gACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCAttt                                                                                                                        <  2:9175476/151‑1 (MQ=255)
                           gACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCAttt                                                                                                                        <  1:9456313/151‑1 (MQ=255)
                           gACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCAttt                                                                                                                        <  1:9160772/151‑1 (MQ=255)
                            aCAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTa                                                                                                                       >  2:7215941/1‑151 (MQ=255)
                             cacggCACCTTCGCCCAGTTGTCAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTAc                                                                                                                      <  1:8217536/148‑1 (MQ=255)
                             cAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTAc                                                                                                                      >  1:8975139/1‑151 (MQ=255)
                               aGGCACCTTCGCCCAGTTGTAAACCGGCGAGGCCGCCAAGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATATACGt                                                                                                                    >  1:7120750/1‑151 (MQ=255)
                                ggCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGtc                                                                                                                   >  2:4527928/1‑151 (MQ=255)
                                   aCCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGtcctc                                                                                                                <  2:7547365/151‑1 (MQ=255)
                                   aCCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGtcctc                                                                                                                >  2:4138842/1‑151 (MQ=255)
                                   aCCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGtcctc                                                                                                                <  1:2501999/151‑1 (MQ=255)
                                    ccTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGtcctcc                                                                                                               <  2:5123718/151‑1 (MQ=255)
                                     cTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTcctccc                                                                                                              >  1:8676213/1‑151 (MQ=255)
                                       tCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTcctccctc                                                                                                            <  1:10361765/151‑1 (MQ=255)
                                       tCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTcctccctc                                                                                                            <  2:4046924/151‑1 (MQ=255)
                                          cccAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTg                                                                                                         >  1:5834135/1‑151 (MQ=255)
                                          cccAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTg                                                                                                         <  1:2198716/151‑1 (MQ=255)
                                           ccAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGt                                                                                                        <  2:9760777/151‑1 (MQ=255)
                                           ccAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGt                                                                                                        <  1:6488362/151‑1 (MQ=255)
                                             aGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAg                                                                                                      >  2:6395659/1‑151 (MQ=255)
                                             aGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAg                                                                                                      <  2:9547194/151‑1 (MQ=255)
                                             aGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTGGTAg                                                                                                      >  2:8015501/1‑151 (MQ=255)
                                              gtggTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCACCGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATGTACGTCCTCCCTCGTGTAGa                                                                                                     <  2:4756692/148‑1 (MQ=255)
                                              gTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGa                                                                                                     <  2:1430168/151‑1 (MQ=255)
                                                  tAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGc                                                                                                 >  2:3022310/1‑151 (MQ=255)
                                                   aaaCCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGCCGGCTCCCGCCGCAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCt                                                                                                >  2:6606325/1‑151 (MQ=255)
                                                    aaCCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTc                                                                                               >  2:2258151/1‑151 (MQ=255)
                                                    aaCCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTc                                                                                               <  1:404936/151‑1 (MQ=255)
                                                    aaCCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTc                                                                                               >  1:9567833/1‑151 (MQ=255)
                                                     aCCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCg                                                                                              <  1:5545339/151‑1 (MQ=255)
                                                     aCCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCg                                                                                              <  1:8540572/151‑1 (MQ=255)
                                                     aCCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCg                                                                                              <  1:6743869/151‑1 (MQ=255)
                                                      ccGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCgg                                                                                             <  2:2695973/151‑1 (MQ=255)
                                                        ggCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTc                                                                                           >  2:5379813/1‑151 (MQ=255)
                                                          cgcgGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGCTCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTATTGTAGACCGCTCGGTCCa                                                                                         <  2:243274/151‑1 (MQ=255)
                                                            cgGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCAcc                                                                                       >  2:6752989/1‑151 (MQ=255)
                                                             ggCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGTAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCAccc                                                                                      <  2:1152269/151‑1 (MQ=255)
                                                              gccgccATGCGGCAGAATGGCGCGCAACGGCGATCCCGCTCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCt                                                                                     <  1:4816008/151‑1 (MQ=255)
                                                               ccgccATGCGGCAGAATGGCGCGCACCGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTc                                                                                    <  1:4508084/151‑1 (MQ=255)
                                                               ccgccATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTc                                                                                    <  1:7812716/151‑1 (MQ=255)
                                                                cgccACGCGGCAGACTGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGCAGGCGCGGCGACC‑GGCCCCAGGACGACGCGAAAACGCATTTACGGCCTCCCTCTTGTAGACCGCTCGGTCCACCcaca                                                                                   <  1:10129047/151‑1 (MQ=255)
                                                                 gccATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCat                                                                                  >  1:1600530/1‑151 (MQ=255)
                                                                  ccATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCata                                                                                 >  2:7884146/1‑151 (MQ=255)
                                                                   cATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCatat                                                                                <  1:1568224/151‑1 (MQ=255)
                                                                        ggCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTgggg                                                                           <  1:8833585/151‑1 (MQ=255)
                                                                         gCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGc                                                                          <  2:4712049/151‑1 (MQ=255)
                                                                             aaTGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGAcc                                                                      <  2:10763364/151‑1 (MQ=255)
                                                                                  cgcgcAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCt                                                                 <  2:10629230/151‑1 (MQ=255)
                                                                                  cgcgcAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCt                                                                 <  2:7332441/151‑1 (MQ=255)
                                                                                   gcgcAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTg                                                                <  2:6832457/151‑1 (MQ=255)
                                                                                   gcgcAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTg                                                                <  1:3483143/151‑1 (MQ=255)
                                                                                   gcgcAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCAGTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTg                                                                >  1:9485861/1‑151 (MQ=255)
                                                                                       aaCGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGAc                                                            >  1:10767274/1‑151 (MQ=255)
                                                                                          ggCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGAcgcg                                                         >  1:755168/1‑151 (MQ=255)
                                                                                          ggCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGAcgcg                                                         >  1:3749485/1‑151 (MQ=255)
                                                                                                cccgatccGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATcaca                                                   <  1:3079973/151‑1 (MQ=255)
                                                                                                   gatccGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAgg                                                >  2:1897089/1‑151 (MQ=255)
                                                                                                      ccGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAAt                                             <  2:7676430/151‑1 (MQ=255)
                                                                                                      ccGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAAt                                             <  2:10322000/151‑1 (MQ=255)
                                                                                                       cGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATc                                            <  2:8122271/151‑1 (MQ=255)
                                                                                                       cGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATc                                            >  1:3965770/1‑151 (MQ=255)
                                                                                                             gggCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACt                                      <  1:7394380/151‑1 (MQ=255)
                                                                                                                 gcccgccTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAg                                  <  2:5605769/151‑1 (MQ=255)
                                                                                                                  cccgccTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAGc                                 <  2:10135447/151‑1 (MQ=255)
                                                                                                                   ccgccTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAGCg                                >  1:8051082/1‑151 (MQ=255)
                                                                                                                     gccTCGACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTGGTAGACCGCTCGGTCCACCCTCATATTGGGGCGCCCGAGCTGGGACGCGTTCAGAAGGAATCGCGACTTGAGCGag                              >  2:705784/1‑150 (MQ=255)
                                                                                                                        tCGCCGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATAGTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAGCGAtctt                           <  2:3263381/151‑1 (MQ=255)
                                                                                                                          gACGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCGCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAGCGAtcttct                         >  2:2807352/1‑151 (MQ=255)
                                                                                                                            cGGCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAGCGATCTtctct                       >  2:8663768/1‑151 (MQ=255)
                                                                                                                             ggCTCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAGCGATCTtctctc                      <  1:2879038/151‑1 (MQ=255)
                                                                                                                                tCCCGCCGAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAGCGATCTTCTCTCccg                   <  2:8490764/151‑1 (MQ=255)
                                                                                                                                 cccGCCCAAGGCGCGGCGAAC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTACTCCCTCTTGTAGACCTCTCGGTCCACACACATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACATGAGCGAGCTTCTCTCccgc                  <  2:6054169/151‑1 (MQ=255)
                                                                                                                                  ccgccgAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAGCGATCTTCTCTCccgcc                 <  1:9702956/151‑1 (MQ=255)
                                                                                                                                  ccgccgAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAGCGATCTTCTCTCccgcc                 <  1:1799022/151‑1 (MQ=255)
                                                                                                                                     ccgAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAGCGATCTTCTCTCCCgccgcc              <  2:89493/151‑1 (MQ=255)
                                                                                                                                       gAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAGCGATCTTCTCTCCCGCCGCCTg            >  1:2010389/1‑151 (MQ=255)
                                                                                                                                       gAAGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAGCGATCTTCTCTCCCGCCGCCTg            <  2:3676915/151‑1 (MQ=255)
                                                                                                                                        aaGGCGCGGCGACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAGCGATCTTCTCTCCCGCCGCCTgg           >  1:6963622/1‑151 (MQ=255)
                                                                                                                                             gcggcgACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAGCGATCTTCTCTCCCGCCGCCTGGCTCTg      >  2:8446551/1‑151 (MQ=255)
                                                                                                                                             gcggcgACC‑GGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAGCGATCTTCTCTCCCGCCGCCTGGCTCTg      >  1:4381551/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
GGCAAGGCGACAAGCTAGTCGCGAAAGGACAAGGCACCTTCGCCCAGTTGTAAACCGGCGCGGCCGCCATGCGGCAGAATGGCGCGCAACGGCGATCCCGATCCGTGCCGGGCGCCCGCCTCGACGGCTCCCGCCGAAGGCGCGGCGACCGGGCCCCAGGACGAAGCGAAAACGCATTTACGTCCTCCCTCTTGTAGACCGCTCGGTCCACCCTCATATTGGGGCGACCGATCTGGGACGCGATCACAAGGAATCCCGACTTGAGCGATCTTCTCTCCCGCCGCCTGGCTCTGCTCG  >  NC_008463/6128483‑6128779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A