Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	10	329.4	8810116	98.1%	8642723	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	2,073,848	Δ2 bp	intergenic (+106/+161)	lysV → / ← xapR	tRNA‑Lys/DNA‑binding transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	2,073,847	0	G	.	100.0%	520.6 / NA	114	intergenic (+105/+163)	lysV/xapR	tRNA‑Lys/DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (37/77); total (37/77)
*	AP012306	2,073,848	0	T	.	100.0%	520.6 / NA	114	intergenic (+106/+162)	lysV/xapR	tRNA‑Lys/DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (37/77); total (37/77)

Only 100 of 122 total aligned reads displayed.

CTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGTGCTGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTATTT  >  AP012306/2073698‑2073987

||

cTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCg                                                                                                                                              >  2:4184823/1‑150 (MQ=32)

 ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgc                                                                                                                                             >  1:1091894/1‑149 (MQ=14)

 ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgc                                                                                                                                             >  2:3405923/1‑149 (MQ=14)

   ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcgg                                                                                                                                           <  2:2382250/150‑4 (MQ=1)

   ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcgg                                                                                                                                           >  1:1507524/1‑147 (MQ=1)

                                                                                                    gAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGa                                       >  1:2778645/1‑150 (MQ=0)

                                                                                                    gAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGa                                       <  1:4293741/150‑1 (MQ=0)

                                                                                                    gAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGa                                       <  2:3707331/150‑1 (MQ=0)

                                                                                                    gAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGa                                       <  1:1764873/150‑1 (MQ=0)

                                                                                                     aaCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGaa                                      >  2:3281713/1‑150 (MQ=2)

                                                                                                     aaCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGaa                                      >  1:1504006/1‑150 (MQ=2)

                                                                                                      aCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGaaa                                     <  1:3088628/150‑1 (MQ=255)

                                                                                                      aCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGaaa                                     >  1:3467075/1‑150 (MQ=255)

                                                                                                       ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAt                                    <  1:3040275/150‑1 (MQ=255)

                                                                                                       ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAt                                    >  2:1593187/1‑150 (MQ=255)

                                                                                                       ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAt                                    <  1:4401006/150‑1 (MQ=255)

                                                                                                        cTGCAGCGGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAtt                                   >  1:4165224/1‑150 (MQ=11)

                                                                                                        cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAtt                                   >  1:4255018/1‑150 (MQ=255)

                                                                                                        cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAtt                                   <  1:2690576/150‑1 (MQ=255)

                                                                                                        cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAtt                                   <  1:564331/150‑1 (MQ=255)

                                                                                                        cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAtt                                   <  2:4193991/150‑1 (MQ=255)

                                                                                                        cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAtt                                   <  1:1154350/150‑1 (MQ=255)

                                                                                                        cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAtt                                   >  2:1041002/1‑150 (MQ=255)

                                                                                                         tGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTg                                  >  2:1387125/1‑150 (MQ=255)

                                                                                                         tGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTg                                  <  2:3150472/150‑1 (MQ=255)

                                                                                                           cagcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCTGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGcc                                <  1:3703545/150‑1 (MQ=11)

                                                                                                           cagcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGcc                                >  1:1546248/1‑150 (MQ=255)

                                                                                                           cagcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGcc                                >  2:760589/1‑150 (MQ=255)

                                                                                                           cagcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGcc                                >  1:2198640/1‑150 (MQ=255)

                                                                                                            agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               <  2:464129/150‑1 (MQ=255)

                                                                                                            agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               >  2:3229411/1‑150 (MQ=255)

                                                                                                            agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               >  1:1387591/1‑150 (MQ=255)

                                                                                                            agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               >  1:292471/1‑150 (MQ=255)

                                                                                                            agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               >  1:2988345/1‑150 (MQ=255)

                                                                                                            agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               <  2:1976504/150‑1 (MQ=255)

                                                                                                            agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               >  1:125001/1‑150 (MQ=255)

                                                                                                             gcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTg                              <  1:1773129/150‑1 (MQ=255)

                                                                                                             gcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTg                              >  1:1673484/1‑150 (MQ=255)

                                                                                                              cagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGa                             <  2:4339210/150‑1 (MQ=255)

                                                                                                               agGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGAt                            <  1:1202551/150‑1 (MQ=255)

                                                                                                               agGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGAt                            >  1:852961/1‑150 (MQ=255)

                                                                                                               agGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGAt                            <  2:508230/150‑1 (MQ=255)

                                                                                                                ggTTTGAGTTGAGCGCAGCGAAACACCGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  2:3259517/150‑1 (MQ=14)

                                                                                                                ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  2:2388563/150‑1 (MQ=255)

                                                                                                                ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           >  1:708112/1‑150 (MQ=255)

                                                                                                                ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  1:3427267/150‑1 (MQ=255)

                                                                                                                 gTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgc                          >  1:2028946/1‑150 (MQ=255)

                                                                                                                 gTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgc                          >  2:2344181/1‑150 (MQ=255)

                                                                                                                  tttGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcg                         <  2:4168267/150‑1 (MQ=255)

                                                                                                                  tttGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcg                         <  1:1311206/150‑1 (MQ=255)

                                                                                                                    tgagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCt                       <  1:2893905/150‑1 (MQ=255)

                                                                                                                     gagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCtt                      <  1:4396395/150‑1 (MQ=255)

                                                                                                                     gagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCtt                      <  2:1476863/150‑1 (MQ=255)

                                                                                                                     gagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCtt                      >  2:1083368/1‑150 (MQ=255)

                                                                                                                      agttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTc                     >  2:773685/1‑150 (MQ=255)

                                                                                                                      agttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTc                     <  2:2694428/150‑1 (MQ=255)

                                                                                                                       gttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCa                    >  1:2487983/1‑150 (MQ=255)

                                                                                                                        ttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCAc                   >  1:2145768/1‑150 (MQ=255)

                                                                                                                        ttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCAc                   <  1:198232/150‑1 (MQ=255)

                                                                                                                        ttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCAc                   <  2:2000764/150‑1 (MQ=255)

                                                                                                                         tgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACt                  <  2:2346486/150‑1 (MQ=255)

                                                                                                                         tgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACt                  <  1:1013655/150‑1 (MQ=255)

                                                                                                                          gagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACtt                 <  2:1260360/150‑1 (MQ=255)

                                                                                                                          gagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACtt                 <  2:1712038/150‑1 (MQ=255)

                                                                                                                          gagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACtt                 <  2:2158994/150‑1 (MQ=255)

                                                                                                                          gagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACtt                 <  1:2250974/150‑1 (MQ=255)

                                                                                                                          gagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACtt                 >  2:2047672/1‑150 (MQ=255)

                                                                                                                           agCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTa                <  2:1007252/150‑1 (MQ=255)

                                                                                                                           agCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTa                <  2:2855911/150‑1 (MQ=255)

                                                                                                                           agCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTa                <  1:3441660/150‑1 (MQ=255)

                                                                                                                           agCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTa                <  1:2983636/150‑1 (MQ=255)

                                                                                                                            gcgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAg               <  1:283690/150‑1 (MQ=255)

                                                                                                                             cgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGc              <  2:930593/150‑1 (MQ=255)

                                                                                                                             cgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGc              <  1:2312639/150‑1 (MQ=255)

                                                                                                                              gcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCa             <  1:1763700/150‑1 (MQ=255)

                                                                                                                              gcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCa             <  1:450494/150‑1 (MQ=255)

                                                                                                                               cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            <  2:3015286/150‑1 (MQ=255)

                                                                                                                               cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            <  2:3638537/150‑1 (MQ=255)

                                                                                                                                aGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGa           <  2:1969023/150‑1 (MQ=255)

                                                                                                                                aGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGa           <  2:1289707/150‑1 (MQ=255)

                                                                                                                                aGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGa           >  2:2609698/1‑150 (MQ=255)

                                                                                                                                aGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGa           <  1:3107300/150‑1 (MQ=255)

                                                                                                                                 gCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGac          <  2:2952538/150‑1 (MQ=255)

                                                                                                                                  cGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGact         <  1:1950197/150‑1 (MQ=255)

                                                                                                                                   gAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGacta        <  2:1312526/150‑1 (MQ=255)

                                                                                                                                     aacaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactact      <  1:1325128/150‑1 (MQ=255)

                                                                                                                                     aacaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactact      >  1:2387232/1‑150 (MQ=255)

                                                                                                                                      acaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGActacta     <  2:1135215/150‑1 (MQ=255)

                                                                                                                                      acaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGActacta     <  1:1410970/150‑1 (MQ=255)

                                                                                                                                      acaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCCGATGCGCTTCACTTAGCAGActacta     <  2:2526557/150‑1 (MQ=17)

                                                                                                                                       caacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCATGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAt    <  1:332626/150‑1 (MQ=18)

                                                                                                                                       caacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAt    >  1:3913434/1‑150 (MQ=255)

                                                                                                                                       caacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAt    <  1:4060452/150‑1 (MQ=255)

                                                                                                                                       caacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAt    <  2:1316160/150‑1 (MQ=255)

                                                                                                                                       caacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAt    <  1:1589248/150‑1 (MQ=255)

                                                                                                                                       caacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAACAGACTACTAt    <  1:3369255/150‑1 (MQ=17)

                                                                                                                                         acGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAttt  >  1:1272361/1‑150 (MQ=255)

                                                                                                                                         acGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAttt  <  1:1091125/150‑1 (MQ=255)

                                                                                                                                         acGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAttt  >  1:1996533/1‑150 (MQ=255)

                                                                                                                                         acGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAttt  >  2:1566822/1‑150 (MQ=255)

||

CTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGTGCTGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTATTT  >  AP012306/2073698‑2073987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A