Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R3 46 423.2 7875468 99.1% 7804588 124.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA AssemblyC74_contig_1 443,385 G→A intergenic (+514/–) rasttk_feature_annotation_tool=annotate_proteins_si milarity → / – Conserved hypothetical protein ArsC related/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AssemblyC74_contig_1443,3850GA79.7% 2270.8 / inf 1295intergenic (+514/–)rasttk_feature_annotation_tool=annotate_proteins_si milarity/–Conserved hypothetical protein ArsC related/–
Reads supporting (aligned to +/- strand):  ref base G (126/136);  new base A (444/586);  total (571/724)
Fisher's exact test for biased strand distribution p-value = 1.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1322 total aligned reads displayed.

ATAAGTTATGATAAATGAGTTGCTGTAAGATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGA  >  AssemblyC74_contig_1/443253‑443516
                                                                                                                                     |                                                                                                                                   
          aTAAATGAGTTGCTGTAAGATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCtgat                                                                                                                         >  1:2875773/1‑135 (MQ=40)
             aaTGAGTTGCTGTAAGATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGaa                                                                                                                      <  2:2842373/135‑1 (MQ=255)
                  gTTGCTGTAAGATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGt                                                                                                                    <  1:885031/132‑1 (MQ=255)
                  gTTGCTGGAAGATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAATAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGa                                                                                                                 >  1:1380580/1‑135 (MQ=255)
                  gTTGCTGAAAGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTa                                                                                                                   >  1:1434848/1‑134 (MQ=11)
                     gCTGTAAGATTTCGAAAAACTAAATA‑AAAAAAACTTATAAAAAAGAGTTGAAAATTACTTGTCTCTCAGATAAGATATAAAAGTAGCTACGGCAGCCAAAAACCAATCGGGTAACACTCCACATGAAGTAGAACt                                                                                                              >  2:2587999/1‑135 (MQ=255)
                     gCTGTAAGATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCt                                                                                                              >  2:1728680/1‑135 (MQ=255)
                      ccgaaaGATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCtt                                                                                                             >  1:1676128/5‑135 (MQ=11)
                        gaaaGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCttt                                                                                                            >  2:652381/3‑135 (MQ=11)
                        gaaaGATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTg                                                                                                           >  1:2553541/3‑135 (MQ=11)
                         aaaGATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGa                                                                                                          <  1:64403/134‑1 (MQ=11)
                           aGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGaa                                                                                                         >  2:1391647/1‑135 (MQ=14)
                           aGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGa                                                                                                          <  1:1814279/134‑1 (MQ=14)
                           aGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGa                                                                                                          <  1:3823470/134‑1 (MQ=14)
                           aGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGa                                                                                                          <  2:2933760/134‑1 (MQ=14)
                               ttCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACt                                                                                                     <  2:2272618/135‑1 (MQ=14)
                               ttCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACt                                                                                                     <  1:414677/135‑1 (MQ=14)
                               ttCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACt                                                                                                     <  1:1499752/135‑1 (MQ=14)
                               ttCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACt                                                                                                     <  2:1089971/135‑1 (MQ=14)
                               ttCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACt                                                                                                     <  1:262012/135‑1 (MQ=14)
                               ttCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACt                                                                                                     <  2:155107/134‑1 (MQ=255)
                               ttCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACt                                                                                                     <  2:1960296/134‑1 (MQ=255)
                               ttCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACt                                                                                                     <  2:3847654/134‑1 (MQ=255)
                               ttCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACt                                                                                                     <  2:52765/134‑1 (MQ=14)
                               ttCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACt                                                                                                     <  1:3396558/134‑1 (MQ=14)
                               ttCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACt                                                                                                     <  1:3147000/134‑1 (MQ=255)
                                  gAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAAc                                                                                                 >  1:1752266/1‑135 (MQ=14)
                                   aaaaaCGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAAc                                                                                                 >  2:2191353/1‑135 (MQ=17)
                                   aaaaaCGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAAACGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGaaa                                                                                                 >  2:1621070/1‑134 (MQ=14)
                                   aaaaaCGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACa                                                                                                >  1:1949254/1‑135 (MQ=255)
                                   aaaaaCGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACa                                                                                                >  1:2999068/1‑135 (MQ=255)
                                     aaaCGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACaa                                                                                               <  1:2098839/134‑1 (MQ=14)
                                      aaCGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAg                                                                                             >  1:1087151/1‑135 (MQ=255)
                                        cGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTa                                                                                           <  1:3547456/135‑1 (MQ=255)
                                        cGAAATA‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTATCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTa                                                                                           <  2:1793386/135‑1 (MQ=255)
                                          aaaTC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTaa                                                                                          >  1:726394/1‑134 (MQ=255)
                                              cgaaaaaaaCTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACa                                                                                      <  1:2328159/133‑1 (MQ=18)
                                                gaaaaaaCTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACaaa                                                                                    <  2:3040256/134‑1 (MQ=18)
                                                aaaaaaaCTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACaaa                                                                                    <  1:2042487/135‑1 (MQ=18)
                                                aaaaaaaCTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACaaa                                                                                    <  1:120397/135‑1 (MQ=18)
                                                 aaaaaaCTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAAc                                                                                   <  1:489549/135‑1 (MQ=255)
                                                 aaaaaaCTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAAc                                                                                   <  2:1275525/135‑1 (MQ=255)
                                                 aaaaaaCTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAAc                                                                                   <  1:2973689/135‑1 (MQ=255)
                                                  aaaaaCTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAAc                                                                                   <  1:2410408/134‑1 (MQ=18)
                                                  aaaaaCTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAAc                                                                                   <  1:3876504/134‑1 (MQ=18)
                                                   aaaaCTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAAc                                                                                   <  2:2979076/133‑1 (MQ=18)
                                                      aCTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAAt                                                                              <  2:2469107/135‑1 (MQ=255)
                                                           tAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTa                                                                         <  2:3415726/135‑1 (MQ=21)
                                                           tAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTa                                                                         <  2:1174129/135‑1 (MQ=21)
                                                             aaaaaGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAg                                                                        >  2:2409059/1‑134 (MQ=21)
                                                                       gACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTg                                                               <  2:829951/133‑1 (MQ=255)
                                                                       gACAATCGCTTGTCTCTCTGTTATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACTCTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTg                                                               <  2:2746400/133‑1 (MQ=25)
                                                                       gACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTg                                                               <  2:759256/133‑1 (MQ=25)
                                                                       gACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTg                                                               <  2:3214634/133‑1 (MQ=25)
                                                                          aaTTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGattca                                                          >  1:3751753/1‑135 (MQ=255)
                                                                             cgtttGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGattcaatt                                                       <  1:1909374/132‑1 (MQ=37)
                                                                                  gTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGa                                                  >  2:2023905/1‑135 (MQ=255)
                                                                                    ctctctGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGac                                                 >  1:2470281/1‑134 (MQ=255)
                                                                                    ctctctGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGaca                                                >  2:1497633/1‑135 (MQ=37)
                                                                                     tctctGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGacaa                                               >  1:3312177/1‑135 (MQ=255)
                                                                                     tctctGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGacaa                                               >  2:2142912/1‑135 (MQ=255)
                                                                                       tctGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACt                                                                                                     >  2:3912860/1‑79 (MQ=255)
                                                                                       tctGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGacaaca                                             >  1:79729/1‑135 (MQ=255)
                                                                                          gatatgatatAAAAGTTTAAACAGCAGCCAAAAACGAATCGGGAAACAAAACCGATGAAGTATAACGTTGAAAAATGAAACAAGTAAGACAAACAAAATTTGTAGGGCGTCGTTATTCAATTAAAGAAaacaaaa                                          >  2:2094475/1‑134 (MQ=16)
                                                                                               gatatAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAAc                                                                                   >  2:908975/1‑89 (MQ=25)
                                                                                                   taAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACAtttttaac                                 >  1:271868/1‑132 (MQ=38)
                                                                                                    aaaaGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAAc                                >  2:233866/1‑135 (MQ=255)
                                                                                                    aaaaGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACAtttttaaca                                <  1:1006850/135‑5 (MQ=255)
                                                                                                    aaaaGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACAtttttaaca                                <  2:2643131/135‑5 (MQ=255)
                                                                                                    aaaaGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACAtttttaaca                                >  2:1852840/1‑131 (MQ=255)
                                                                                                    aaaaGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACAtttttaaca                                <  1:1621070/135‑5 (MQ=255)
                                                                                                    aaaaGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACAtttt                                     <  1:1575802/130‑1 (MQ=255)
                                                                                                     aaaTTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAAcaa                              <  1:2199794/135‑1 (MQ=255)
                                                                                                     aaaGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAAca                               <  1:3079800/135‑1 (MQ=255)
                                                                                                     aaaGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAAcaa                              <  2:1908083/135‑1 (MQ=255)
                                                                                                      aaGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAAcaag                             >  1:1143739/1‑135 (MQ=255)
                                                                                                        gTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAAcaagc                            >  2:470806/1‑135 (MQ=255)
                                                                                                        gTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTg                                                               >  1:3713907/1‑100 (MQ=255)
                                                                                                        gTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGacaat                                              <  1:3255737/117‑2 (MQ=255)
                                                                                                        gTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAAcaagca                           >  2:1023999/1‑135 (MQ=255)
                                                                                                             tACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGAcc                                                                                                               >  2:3349953/1‑47 (MQ=17)
                                                                                                              acagCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGattcaatt                                                       >  2:2684183/4‑102 (MQ=255)
                                                                                                              acagCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAat                     >  1:2251649/4‑135 (MQ=255)
                                                                                                              aCGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACAtttt                                     >  1:3462078/1‑120 (MQ=255)
                                                                                                              aCGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAat                     >  2:1355453/1‑135 (MQ=255)
                                                                                                              aCGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTTACaagcaagcta                      >  2:1438058/1‑133 (MQ=255)
                                                                                                              aCGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAAgcaagcaa                      >  1:593408/1‑135 (MQ=255)
                                                                                                              aCGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAAgcaagcaa                      >  2:2546656/1‑135 (MQ=255)
                                                                                                              aCGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAAgcaagcaa                      >  1:927068/1‑135 (MQ=255)
                                                                                                              aCGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACt                                                                                                     >  1:359925/1‑56 (MQ=255)
                                                                                                              aCGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAAcaa                              <  2:3137226/126‑1 (MQ=255)
                                                                                                              aCGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAGCAAGCAat                     >  2:3670790/1‑135 (MQ=255)
                                                                                                              aCGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAGCAAGCAat                     >  2:700581/1‑135 (MQ=255)
                                                                                                              aCGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAGCAAGCAat                     >  1:1282382/1‑135 (MQ=255)
                                                                                                                       aaaaaCGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACAtttttaaca                                >  2:3274903/1‑112 (MQ=255)
                                                                                                                        aaaaCGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGca            >  2:3502454/1‑134 (MQ=255)
                                                                                                                                  ggggAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTg   >  2:3586719/1‑134 (MQ=255)
                                                                                                                                  gggaAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTg   >  1:2806702/1‑133 (MQ=255)
                                                                                                                                  gggaAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAg                                                                                         >  1:2661598/1‑48 (MQ=255)
                                                                                                                                  gggaAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTg   >  2:3765343/1‑133 (MQ=255)
                                                                                                                                     |                                                                                                                                   
ATAAGTTATGATAAATGAGTTGCTGTAAGATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGA  >  AssemblyC74_contig_1/443253‑443516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 812 total aligned reads displayed.
CAAATAACAAATGATAAGTTATGATAAATGAGTTGCTGTAAGATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAATCGT‑‑‑‑‑  >  AssemblyC74_contig_1/443240‑443529
                                                                                                                                                  |                                                                                                                                                     
           TGATAAGTTATGATAAATGAGTTGCTGTAAGATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAG                                                                                                                                       <  SRR22894673.1078571/151‑1 (MQ=50)
             ATAAGTTATGATAAATGAGTTGCTGTAAGATTTCTAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGT                                                                                                                                      <  SRR22894673.3547044/150‑1 (MQ=48)
               AAGTTATGATAAATGAGTTGCTGAAAGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAG                                                                                                                                    <  SRR22894673.4021769/151‑1 (MQ=0)
                AGTTATGATAAATGAGTTGCTGAAAGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGA                                                                                                                                   >  SRR22894673.1375274/1‑151 (MQ=1)
                 TTTATGATAAATGAGTTGCTGTAAGATTTATAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAATTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACC                                                                                                                                 <  SRR22894673.275762/151‑1 (MQ=55)
                 GTTATGATAAATGAGTTGCTGAAAGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGA                                                                                                                                   >  SRR22894673.1004639/1‑150 (MQ=1)
                  TTATGATAAATGAGTTGCTGAAAGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACC                                                                                                                                 <  SRR22894673.549131/151‑1 (MQ=0)
                  TTATGATAAATGAGTTGCTGAAAGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACC                                                                                                                                 <  SRR22894673.1922549/151‑1 (MQ=0)
                  TTATGATAAATGAGTTGCTGAAAGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACC                                                                                                                                 <  SRR22894673.3295361/151‑1 (MQ=0)
                  TTATGATAAATGAGTTGCTGAAAGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACC                                                                                                                                 <  SRR22894673.873037/151‑1 (MQ=4)
                    ATGATAAATGAGTTGCTGAAAGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTT                                                                                                                               >  SRR22894673.3061592/1‑151 (MQ=8)
                     TGATAAATGAGTTGCTGTAAGATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTG                                                                                                                             >  SRR22894673.3057084/1‑151 (MQ=60)
                     TGATAAATGAGTTGCTGGAAGATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTG                                                                                                                             >  SRR22894673.3189475/1‑151 (MQ=1)
                     TGATAAATGAGTTGCTGGAAGATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTG                                                                                                                             >  SRR22894673.3811889/1‑151 (MQ=1)
                     TGATAAATGAGTTGCTGAAAGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTT                                                                                                                              >  SRR22894673.3513929/1‑151 (MQ=2)
                     TGATAAATGAGTTGCTGAAAGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTT                                                                                                                              >  SRR22894673.208066/1‑151 (MQ=2)
                      GATAAATGAGTTGCTGAAAGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTG                                                                                                                             >  SRR22894673.3678628/1‑151 (MQ=14)
                       ATAAATGAGTTGCTGAAAGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGA                                                                                                                            >  SRR22894673.1880489/1‑151 (MQ=16)
                        TAAATGAGTTGCTGTAAGATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTGGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAA                                                                                                                          <  SRR22894673.3135492/151‑1 (MQ=60)
                        TAAATGAGTTGCTGGAAGATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAA                                                                                                                          >  SRR22894673.1544453/1‑151 (MQ=7)
                        TAAATGAGTTGCCGAAAGATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAA                                                                                                                          <  SRR22894673.3124312/151‑1 (MQ=0)
                        TAAATGAGTTGCCGAAAGATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAA                                                                                                                          <  SRR22894673.2624444/151‑1 (MQ=0)
                        TAAATGAGTTGCCGAAAGATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAA                                                                                                                          <  SRR22894673.2989984/151‑1 (MQ=0)
                         AAATGAGTTGCTGAAAGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAA                                                                                                                          <  SRR22894673.1656632/151‑1 (MQ=8)
                           ATGAGTTGCTGAAAGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAAC                                                                                                                        <  SRR22894673.248111/151‑1 (MQ=14)
                                TTGCCGAAAGATATCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAAC                                                                                                                   <  SRR22894673.499105/150‑1 (MQ=15)
                                  GCTGTAAGATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTATGAAAACTGAACAAA                                                                                                                >  SRR22894673.3369054/1‑151 (MQ=60)
                                  GCTGGAAGATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAA                                                                                                                >  SRR22894673.2438083/1‑151 (MQ=30)
                                  GCTGAAAGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAA                                                                                                                 >  SRR22894673.393759/1‑151 (MQ=35)
                                  GCTGAAAGATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAA                                                                                                                 >  SRR22894673.1057022/1‑151 (MQ=35)
                                   CTGTAAGATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACCATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACC                                                                                                                                 <  SRR22894673.2024245/133‑1 (MQ=59)
                                   CTGTAAGATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAG                                                                                                               >  SRR22894673.366643/1‑151 (MQ=60)
                                    cgaAAGATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGT                                                                                                              <  SRR22894673.1173671/148‑1 (MQ=25)
                                    TGTAAGATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGT                                                                                                              <  SRR22894673.2696806/151‑1 (MQ=60)
                                    TGTAAGATTTAGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGT                                                                                                              <  SRR22894673.3469931/151‑1 (MQ=50)
                                     GTAAGATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTA                                                                                                             >  SRR22894673.1114186/1‑151 (MQ=60)
                                     GTAAGATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATG                                                                                                                                          <  SRR22894673.2030266/122‑1 (MQ=38)
                                     GGAAGATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTA                                                                                                             >  SRR22894673.1824656/1‑151 (MQ=42)
                                         TATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.3537839/151‑1 (MQ=60)
                                         GATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGA                                                                                                          >  SRR22894673.744648/1‑150 (MQ=60)
                                          ATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.2741598/151‑1 (MQ=40)
                                          ATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.2141490/151‑1 (MQ=40)
                                          ATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.94397/151‑1 (MQ=40)
                                          ATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.424648/151‑1 (MQ=40)
                                          ATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.1474603/151‑1 (MQ=40)
                                          ATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.118127/151‑1 (MQ=50)
                                          ATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.125398/151‑1 (MQ=50)
                                          ATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.404003/151‑1 (MQ=50)
                                          ATTTCGAAAAACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.2944305/151‑1 (MQ=50)
                                          ATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.3414336/150‑1 (MQ=55)
                                          ATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.3039801/150‑1 (MQ=55)
                                          ATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.3240957/150‑1 (MQ=55)
                                          ATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.2110273/150‑1 (MQ=55)
                                          ATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.1493427/150‑1 (MQ=34)
                                          ATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.3557807/150‑1 (MQ=34)
                                          ATTTCGAAAAACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.1221078/150‑1 (MQ=34)
                                          ATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.2900015/150‑1 (MQ=60)
                                          ATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.2593446/150‑1 (MQ=60)
                                          ATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.1744273/150‑1 (MQ=60)
                                                   AACGAAATC‑GAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATG                                                                                               <  SRR22894673.79796/151‑1 (MQ=60)
                                                   AACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATG                                                                                               <  SRR22894673.3218681/151‑1 (MQ=60)
                                                    ACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATG                                                                                               <  SRR22894673.1755610/151‑1 (MQ=56)
                                                    ACGAAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATG                                                                                               <  SRR22894673.1810110/151‑1 (MQ=56)
                                                       AAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGT                                                                                            <  SRR22894673.2360943/151‑1 (MQ=60)
                                                       AAATCGAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTG                                                                                             <  SRR22894673.926690/150‑1 (MQ=60)
                                                          TC‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGG                                                                                        <  SRR22894673.891364/151‑1 (MQ=60)
                                                          TC‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGG                                                                                        <  SRR22894673.2773457/151‑1 (MQ=60)
                                                           cgAAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGG                                                                                        <  SRR22894673.3902527/149‑1 (MQ=60)
                                                           C‑GAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGC                                                                                       <  SRR22894673.1779438/151‑1 (MQ=60)
                                                           C‑GAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGC                                                                                       <  SRR22894673.1286686/151‑1 (MQ=60)
                                                           C‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGC                                                                                       <  SRR22894673.620796/151‑1 (MQ=60)
                                                             tgggctcggagatgtgtataagattcagtcttttgtctataTGATATGATATAAAAGTTGATACAGCAGACAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGC                                                                                       <  SRR22894673.2817067/109‑1 (MQ=60)
                                                             AAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCG                                                                                      >  SRR22894673.1616782/1‑151 (MQ=60)
                                                             AAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCG                                                                                      <  SRR22894673.2177145/151‑1 (MQ=60)
                                                             AAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGC                                                                                       <  SRR22894673.1039649/150‑1 (MQ=60)
                                                             AAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGC                                                                                       <  SRR22894673.2391425/150‑1 (MQ=60)
                                                             AAAAAAACTTTTAAAAAAGAGTTGACAATCGTTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGC                                                                                       <  SRR22894673.3975575/150‑1 (MQ=60)
                                                             AAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGC                                                                                       <  SRR22894673.1936197/150‑1 (MQ=60)
                                                             AAAAAAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGC                                                                                       <  SRR22894673.2300598/150‑1 (MQ=60)
                                                             AAAAAAACTTTTAAAAAAGAGTTGACAATCGATTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGC                                                                                       <  SRR22894673.1977935/150‑1 (MQ=60)
                                                              AAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGC                                                                                       <  SRR22894673.1696872/149‑1 (MQ=60)
                                                                 AAACTTTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTT                                                                                  <  SRR22894673.1983908/151‑1 (MQ=60)
                                                                      TTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTC                                                                             <  SRR22894673.3711850/151‑1 (MQ=60)
                                                                      TTTAAAAAAGAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTC                                                                             <  SRR22894673.2689928/151‑1 (MQ=60)
                                                                         AAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAAT                                                                          >  SRR22894673.3847654/1‑151 (MQ=60)
                                                                           AAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTC                                                                        <  SRR22894673.603018/151‑1 (MQ=60)
                                                                               GAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACAC                                                                                                                                                  >  SRR22894673.1337758/1‑73 (MQ=12)
                                                                               GAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGA                                                                    >  SRR22894673.2536188/1‑151 (MQ=60)
                                                                               GAGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGA                                                                    >  SRR22894673.3198241/1‑151 (MQ=60)
                                                                                AGTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAG                                                                     <  SRR22894673.158727/149‑1 (MQ=60)
                                                                                 GTTGACTATTACTTGTCTATCTGATATGATATAAAAGTTGCTTCGGCAGCCAAAAACGAATAGGGTAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.3832242/112‑1 (MQ=60)
                                                                                 GTTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACA                                                                  >  SRR22894673.1452767/1‑151 (MQ=60)
                                                                                  ttgacgatcgCTTGTCTCTCTGGTATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGAC                                                                   <  SRR22894673.576276/139‑1 (MQ=60)
                                                                                  TTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGAC                                                                   <  SRR22894673.3617658/149‑1 (MQ=60)
                                                                                  TTGACAATCGCTTGTCTCTCTGATATGATATAACAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGAC                                                                   <  SRR22894673.2281323/149‑1 (MQ=60)
                                                                                  TTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGAC                                                                   <  SRR22894673.3485257/149‑1 (MQ=60)
                                                                                  TTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGAC                                                                   <  SRR22894673.2378530/149‑1 (MQ=60)
                                                                                  TTGACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGAC                                                                   <  SRR22894673.484740/149‑1 (MQ=60)
                                                                                   tgacaatcgtTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTC                                                                        >  SRR22894673.2680512/11‑143 (MQ=60)
                                                                                    GACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGTACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGAC                                                                   <  SRR22894673.3857468/147‑1 (MQ=60)
                                                                                    GACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACA                                                               >  SRR22894673.1568161/1‑151 (MQ=60)
                                                                                    GACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTC                                                                             >  SRR22894673.2339088/1‑137 (MQ=60)
                                                                                    GACAATCGCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATG                                                                                               >  SRR22894673.196821/1‑119 (MQ=60)
                                                                                        atcgtTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA                                                           <  SRR22894673.1957399/146‑1 (MQ=60)
                                                                                        atcgCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.827694/101‑1 (MQ=50)
                                                                                        ATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA                                                           >  SRR22894673.3317391/1‑151 (MQ=60)
                                                                                         tcgCTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGT                                                                                                              <  SRR22894673.1469355/96‑1 (MQ=42)
                                                                                         TTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACAT                                                          >  SRR22894673.3822582/1‑151 (MQ=60)
                                                                                               GTCTCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTAA                                                    >  SRR22894673.1679311/1‑151 (MQ=60)
                                                                                                TCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAAC                                                  <  SRR22894673.2406851/151‑1 (MQ=60)
                                                                                                TCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAACCA‑TTTTTAAC                                                  <  SRR22894673.630178/151‑1 (MQ=60)
                                                                                                TCTCTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACATTTTTAA                                                    >  SRR22894673.3003739/1‑150 (MQ=60)
                                                                                                 CTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGC                                                                                       <  SRR22894673.1714462/114‑1 (MQ=60)
                                                                                                  TCTCTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAA                                                >  SRR22894673.967887/1‑151 (MQ=60)
                                                                                                   CTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAG                                               >  SRR22894673.3021526/1‑151 (MQ=60)
                                                                                                   CTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAG                                               >  SRR22894673.2112367/1‑151 (MQ=60)
                                                                                                   CTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAG                                               >  SRR22894673.3783312/1‑151 (MQ=60)
                                                                                                   CTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAG                                               >  SRR22894673.2553263/1‑151 (MQ=60)
                                                                                                   CTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAG                                               >  SRR22894673.693408/1‑151 (MQ=60)
                                                                                                   CTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑ATTTTAACAAG                                               >  SRR22894673.2000631/1‑151 (MQ=60)
                                                                                                   CTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATG                                                                                               <  SRR22894673.2519253/104‑1 (MQ=60)
                                                                                                   CTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTAGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAG                                               >  SRR22894673.1632937/1‑151 (MQ=60)
                                                                                                   CTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAA                                                >  SRR22894673.117117/1‑151 (MQ=60)
                                                                                                   CTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAG                                               >  SRR22894673.3957805/1‑151 (MQ=60)
                                                                                                   CTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAG                                               >  SRR22894673.3982804/1‑151 (MQ=60)
                                                                                                   CTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCTATGAAGTAGACCTTTGAAAAATGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTTATTCAATTCAAtacactaaacatatttaacaag                                                >  SRR22894673.812095/1‑129 (MQ=60)
                                                                                                   CTCTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAG                                               >  SRR22894673.2582887/1‑151 (MQ=60)
                                                                                                   CTCTGATATGATATAAAAGCTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTC                                                                        <  SRR22894673.388309/127‑1 (MQ=60)
                                                                                                     CTGATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAGCA                                             >  SRR22894673.1767985/1‑151 (MQ=60)
                                                                                                     CTGATATGATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATT                                                                              >  SRR22894673.2847911/1‑119 (MQ=60)
                                                                                                       GATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGG                                                                                        <  SRR22894673.720385/107‑1 (MQ=60)
                                                                                                       GATATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAGCAAG                                           >  SRR22894673.3687072/1‑151 (MQ=60)
                                                                                                          ATGATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTGGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAA                                        <  SRR22894673.1867922/151‑1 (MQ=60)
                                                                                                          ATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAGCAAGCAA                                        <  SRR22894673.660575/151‑1 (MQ=60)
                                                                                                          ATGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAGCAAGCAA                                        <  SRR22894673.131429/151‑1 (MQ=60)
                                                                                                           TGATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAAC                                                  >  SRR22894673.356962/1‑140 (MQ=60)
                                                                                                            GATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTC                                                                                                                                                <  SRR22894673.43851/46‑1 (MQ=27)
                                                                                                            GATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATA                                      >  SRR22894673.3080365/1‑151 (MQ=60)
                                                                                                            GATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAAC                                                                                                                   <  SRR22894673.3435835/75‑1 (MQ=44)
                                                                                                             ATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATAT                                     >  SRR22894673.804429/1‑151 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATAT                                     >  SRR22894673.910142/1‑151 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATAT                                     >  SRR22894673.2460562/1‑151 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGAC                                                                   <  SRR22894673.1262455/122‑1 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATT                                                                         <  SRR22894673.2152434/116‑1 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTC                                                                             <  SRR22894673.1128135/112‑1 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGC                                                                                       >  SRR22894673.15073/1‑102 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATAT                                     >  SRR22894673.363978/1‑151 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATAT                                     >  SRR22894673.529774/1‑151 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATAT                                     >  SRR22894673.2302818/1‑151 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATAT                                     >  SRR22894673.1409036/1‑151 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATAT                                     >  SRR22894673.3879401/1‑151 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         <  SRR22894673.646207/84‑1 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAGCAAGCAATAT                                     >  SRR22894673.3386760/1‑151 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAGCAAGCAATAT                                     >  SRR22894673.3743840/1‑151 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAGCAAGCAATAT                                     >  SRR22894673.2917200/1‑151 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAGCAAGCAATAT                                     >  SRR22894673.1554523/1‑151 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAGCAAGCAATAT                                     >  SRR22894673.3355636/1‑151 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACGGAAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACA                                                 <  SRR22894673.2655021/139‑1 (MQ=60)
                                                                                                             ATATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATAT                                     >  SRR22894673.1425482/1‑151 (MQ=60)
                                                                                                              TATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATG                                    <  SRR22894673.463953/151‑1 (MQ=60)
                                                                                                              TATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATG                                    <  SRR22894673.1100931/151‑1 (MQ=60)
                                                                                                               ATAAAAGTTGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGC                                   <  SRR22894673.216313/151‑1 (MQ=60)
                                                                                                                TAAAATTTGCTACGGCAGCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCT                                  <  SRR22894673.2255626/151‑1 (MQ=60)
                                                                                                                TAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAAGCAAGCAATATGC                                   <  SRR22894673.3158126/151‑1 (MQ=60)
                                                                                                                 AAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAAGCAAGCAATATGCT                                  <  SRR22894673.929261/151‑1 (MQ=60)
                                                                                                                  AAAGTTGCTACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAG                                <  SRR22894673.731264/151‑1 (MQ=60)
                                                                                                                       TGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAAGCAAGCAATATGCTAGCAAA                            >  SRR22894673.961244/1‑151 (MQ=60)
                                                                                                                       TGCTACAGCAGCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAA                            >  SRR22894673.1705572/1‑150 (MQ=60)
                                                                                                                           ACGGCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAAT                       <  SRR22894673.1527448/151‑1 (MQ=60)
                                                                                                                              GCAGCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTG                     <  SRR22894673.3926962/150‑1 (MQ=60)
                                                                                                                                 GCCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAG                   >  SRR22894673.2877729/1‑149 (MQ=60)
                                                                                                                                 GCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAG                   >  SRR22894673.842616/1‑149 (MQ=60)
                                                                                                                                 GCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCA                              <  SRR22894673.115387/138‑1 (MQ=60)
                                                                                                                                 GCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATG                                    <  SRR22894673.3447468/132‑1 (MQ=60)
                                                                                                                                 GCCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         >  SRR22894673.2729089/1‑64 (MQ=60)
                                                                                                                                 GCCAAAAACGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAG                   >  SRR22894673.3049286/1‑149 (MQ=60)
                                                                                                                                  CCAAAAATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGC                  <  SRR22894673.2506109/149‑1 (MQ=60)
                                                                                                                                  CCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTT                >  SRR22894673.2666985/1‑151 (MQ=60)
                                                                                                                                  CCAAAAACGAATCGGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAG                   <  SRR22894673.2807001/148‑1 (MQ=60)
                                                                                                                                        ATGAATCGGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAAT          <  SRR22894673.2710032/151‑1 (MQ=60)
                                                                                                                                         CGAATCGGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGAC                                                                                                         >  SRR22894673.449094/1‑56 (MQ=60)
                                                                                                                                           AATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAATCG        >  SRR22894673.247632/1‑151 (MQ=60)
                                                                                                                                           AATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAATCG        <  SRR22894673.2786240/151‑1 (MQ=60)
                                                                                                                                               GGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAATCGTaat    >  SRR22894673.1357438/1‑148 (MQ=60)
                                                                                                                                               GGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAATCGTaag    >  SRR22894673.2797551/1‑148 (MQ=60)
                                                                                                                                               GGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAAGCAAGCAATATG                                    >  SRR22894673.166144/1‑119 (MQ=60)
                                                                                                                                               GGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAATCGcaaga   >  SRR22894673.2103194/1‑146 (MQ=60)
                                                                                                                                               GGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAATCGcaaga   >  SRR22894673.3644188/1‑146 (MQ=60)
                                                                                                                                               GGGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAATCGcaaga   >  SRR22894673.1834535/1‑146 (MQ=60)
                                                                                                                                               GGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAATCGcaaga   >  SRR22894673.261749/1‑146 (MQ=60)
                                                                                                                                               GGGAAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAATCGcaaga   >  SRR22894673.1848888/1‑146 (MQ=60)
                                                                                                                                               GGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAATCGcaaga   >  SRR22894673.3115318/1‑146 (MQ=60)
                                                                                                                                               GGGAAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATG                                    >  SRR22894673.3491898/1‑118 (MQ=60)
                                                                                                                                                GGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAATCGcaagat  >  SRR22894673.2351968/1‑145 (MQ=60)
                                                                                                                                                GGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAATCGcaagat  >  SRR22894673.3915190/1‑145 (MQ=60)
                                                                                                                                                GGAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGAC                                                                   <  SRR22894673.325826/87‑1 (MQ=60)
                                                                                                                                                 GAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAATAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAATCGcaagat  <  SRR22894673.1797866/150‑7 (MQ=60)
                                                                                                                                                 GAAACACTCTCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTATTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAATCGcaagat  <  SRR22894673.3395948/150‑7 (MQ=60)
                                                                                                                                                  GAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAATCGTaagat  <  SRR22894673.2205133/150‑6 (MQ=60)
                                                                                                                                                  AAACACTCCCGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACA‑TTTTTAACAAGCAAGCAATATGC                                   >  SRR22894673.3210121/1‑116 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
CAAATAACAAATGATAAGTTATGATAAATGAGTTGCTGTAAGATTTCGAAAAACGAAATC‑AAAAAAACTTTTAAAAAAGAGTTGACAATTACTTGTCTCTCTGATATGATATAAAAGTTGCTACGGCAGCCAAAAACGAATCGGGGAACACTCCTGATGAAGTAGACCTTTGAAAACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTCTTGATTCAATTCAAGACAACAAACATTTTTTAACAAGCAAGCAATATGCTAGCAAACAAATTGAGCTTAACAATCGT‑‑‑‑‑  >  AssemblyC74_contig_1/443240‑443529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑