Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F9 I0 R1 113 83.2 1840812 91.6% 1686183 224.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,228,796 T→G 13.7% V356G (GTG→GGG)  yjbB → putative Na+/Pi‑cotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,228,7960TG13.7% 257.2 / 3.7 101V356G (GTG→GGG) yjbBputative Na+/Pi‑cotransporter
Reads supporting (aligned to +/- strand):  ref base T (48/34);  new base G (12/5);  total (62/39)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

TCCACGTCTTCTACAACCTTGTACGTTGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAAC‑CTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCA  >  NC_000913/4228562‑4229034
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                
tcccctttttttgacatcTTGTTCGTTTGCTGG‑TTATGCTGCCATTTGTGGAACCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATg                                                                                                                                                                                                                                              <  2:92010/222‑1 (MQ=255)
agggacagttttcaacccTTTTTCGTTGCCGGG‑TCATGCTGCTATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATg                                                                                                                                                                                                                                              <  2:596079/223‑1 (MQ=255)
     gTCTTCTACAACCTTGTACGTTGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACgg                                                                                                                                                                                                                                         <  1:483923/239‑1 (MQ=255)
        ttctACAACCTTGTACGTTGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGCA‑CGCCCCGCGCGAAACCCTGCGCATTGGCGACGCCTTGGAACAGCTGATGGAAGGGTTGAATAAAGGGATgaccggaga                                                                                                                                                                                                                                      >  1:266619/1‑231 (MQ=255)
        ttctACAACCTTGTACGTTGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGGTGCACGGTGa                                                                                                                                                                                                                                      >  1:577863/1‑239 (MQ=255)
            accaCCGTGTACTTTGCCTGG‑TTGTGCTGCCATTTGTTGGCCTGATGGCACGGTTTTG‑CCAAACGATTATGCGCGATGGA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCa                                                                                                                                                                                                                                  <  1:764405/236‑1 (MQ=255)
                ccTTGTACGTTGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCCGGGAAAAGATGATGGAGGGGGTGAAAAAAGGGATGCCCGGGGAGCCACggg                                                                                                                                                                                                                              >  2:863983/1‑238 (MQ=255)
                 cTTGTACGTTGCCTGGTTTTTGCT‑CCTTTTGTTGACCCGTGGGCCTGGTTTTG‑CAAAACGATTTATCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGGGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCa                                                                                                                                                                                                                             <  2:430905/239‑1 (MQ=255)
                  ttGTACGTTGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGGAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAg                                                                                                                                                                                                                            >  1:420777/1‑239 (MQ=255)
                   tGTACGTTGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGTTGCACGGTGAGc                                                                                                                                                                                                                                    >  1:596079/1‑230 (MQ=255)
                   tGTACGTTGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGAACGGTGAGCCACGGCAgg                                                                                                                                                                                                                           >  1:73791/1‑239 (MQ=255)
                   tGTACGTTGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGAGGGAAGGGTTGAATAAAGGGATGCACGGGGAGCCACGGCggg                                                                                                                                                                                                                           >  1:94637/1‑239 (MQ=255)
                   tGTACGTTGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGAAGGCAGGGTTGAATAAAGTGATGCACGGCGAGCCACGGCAgg                                                                                                                                                                                                                           >  1:577297/1‑239 (MQ=255)
                   tGTACGTTGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGACGATGGAAGGGTTGAATAAAAGGATGCACGGGGAGCCACGGGAgg                                                                                                                                                                                                                           >  2:294749/1‑239 (MQ=255)
                   tGTACGTTGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCCGCCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGAGGTGAACGGTGAACCACGGCggg                                                                                                                                                                                                                           >  2:333037/1‑239 (MQ=255)
                   tGTACGTTGCCTGG‑TCATGCTGCCACTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGCTACCATGCGCATTGGCGACCCCATGGAACAGCTGTTGGAAGGGTTGAATAAAGGTATGCACGGTGAGCCAGGGCAgg                                                                                                                                                                                                                           >  1:463127/1‑239 (MQ=255)
                    gTACGTTGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGGCAGCGCGCTGGATACGCACACCCTTGCTCTGGCGCA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGCCGCCCAGGAAAAGATGATGGAAGGGTTGGATAAAGGGATGCACGGGGGGACACgggcggg                                                                                                                                                                                                                          >  2:487892/1‑234 (MQ=255)
                       cGTTGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCCCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGAGGATGGCAGGGTTGAATAAAGTGCTGCACGGTGTGCCCCGGGGGGA‑Gaa                                                                                                                                                                                                                      >  1:91746/1‑239 (MQ=255)
                          tGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑Gaaaaa                                                                                                                                                                                                                   >  1:753378/1‑237 (MQ=255)
                          tGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGAGGCACGGTGAGCCACGGCAGGG‑Gaaaaa                                                                                                                                                                                                                   >  1:459088/1‑237 (MQ=255)
                          tGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCCCGCGAAACCCTGCACATTGGCGACGCCCTGGAACAGGAGATGGAAGGGTAGAATAAAGAGAGTGACGGTGCGACACGGCAGGA‑Gaaaaa                                                                                                                                                                                                                   >  2:14892/1‑237 (MQ=255)
                           gCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGTTGAA‑CCGGAACTGGATACCCAACTACAGCCTAAACATCTGGATGTCACCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCCGCCGAAACCCCTCCCATTGGCGACGCCATGGAACAAGTGATGGGAGGGTCGGATAAAGGGATGCACGGGAAGCCCGCGACGGA‑GAAagag                                                                                                                                                                                                                  >  2:294678/1‑239 (MQ=255)
                           gCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTAAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCCCGCGAACCCCTCGGCATTGGCGACGCCATGGAACGGAGGATGGGAGGGTTGAATAAAGGGAGGCACGGGGGGCAACGCAAGGA‑GAAagag                                                                                                                                                                                                                  >  2:673121/1‑239 (MQ=255)
                           gCCTGG‑TAATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCACGCGCGGAACCCTAGGCATTGGCCACCCCATGGACAAGCAGTTGGAAGGGGTGATAAAAAGGATGCCCTGGGAGCCAC‑GCAGGAGGAAagag                                                                                                                                                                                                                  >  2:33735/1‑239 (MQ=255)
                               gg‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGAAGATGGAAGGGTTGATAAAAGGGATGCACGGTGAGCCCCGGAGGGA‑GAAAAAGGTgg                                                                                                                                                                                                              >  2:840078/1‑238 (MQ=255)
                                     tgctgcCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAg                                                                                                                                                                                                         <  1:840078/239‑1 (MQ=255)
                                             ttttgtgccccGGTGGCCCGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAga                                                                                                                                                                                                 <  2:252011/231‑1 (MQ=255)
                                               tgttgACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGGGATGCCCGGTGAGCCACGGCAGGG‑GAAAGAGCTGGGTAAGCTGGCaaagg                                                                                                                                                                                               >  1:444733/1‑235 (MQ=255)
                                                 tttcccccggttgcccgTTTTGTCAAAACGATTATGCGCGATGGA‑CCGGAACTGGATACCCAGCTACGGCCGAAACATCGGGATGGCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAgatga                                                                                                                                                                                              <  2:463127/223‑1 (MQ=255)
                                                    gcccGATGGCCCGGTTTTG‑GAAAACGATTAGTCACGATGAA‑CCGGAACTGGAGTCCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGatat                                                                                                                                                                                           <  1:725197/237‑1 (MQ=255)
                                                    aCCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCGGATGATATc                                                                                                                                                                                          >  1:48060/1‑239 (MQ=255)
                                                     cccGATGGCACGGTTTTG‑CAAAACGATTAGTCGCGATGAC‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGGCAGCGCGCTGGATACGCCCACGGTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCa                                                                                                                                                                                         <  1:508582/239‑1 (MQ=255)
                                                      ccgggggCAAGGTTTTG‑AAAAACGGTTTTTCGCGATGGA‑CCGGAAATGGATCCCCAGGTACGGCCTAAACATCTGGATGTCAGGGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCa                                                                                                                                                                                         <  2:73791/233‑1 (MQ=255)
                                                       cggtGGCACGGTTTGG‑CAAAACGATTATTCGCGATGAA‑CCGGGACTGGATACCCAGCCACGGTCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAAc                                                                                                                                                                                       <  2:124696/236‑1 (MQ=255)
                                                       cGATGGCACGGTTTTG‑GAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAAc                                                                                                                                                                                       <  1:159114/239‑1 (MQ=255)
                                                          tggccaggTTTTC‑AAAACCGTTTTTTCGGGACGAA‑CCGGAACTGGATCCCCAGATACGGCCTAAACATCTGGATGTCAGCGCGCTGGATCCGCCCACGCTTGCTCTGGGGAA‑CGCCGCGCGCGAAACCCTGCCCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCTCGGCAGGA‑GAAAGAGCTGCGTAAGCTGGAAGATGATATCAACGtt                                                                                                                                                                                    <  2:883762/233‑1 (MQ=255)
                                                          tGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATTCCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAAc                                                                                                                                                                                       <  1:863983/236‑1 (MQ=255)
                                                                   tttt‑CAAAACCTTTTTTCGGGGTGGACCCGGAACGGGACCCCCAGCGCGGGCCAAAACATCGGGAGGGCAGGGCGGGGGATCCCCCCCGGCTTGCTTGGGCAAA‑CGCCGCGGGCAAACCCCGGCGGATGGGCGACGCAAGGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCCCGGCAGGA‑AAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTacac                                                                                                                                                                            <  2:48060/239‑1 (MQ=255)
                                                                   tttG‑AAAAAGGATTATTCGCGATGAC‑CCGGAACGGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCGGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTacac                                                                                                                                                                            <  1:367374/238‑1 (MQ=255)
                                                                        cAAAACTATTAGTCGCGATTAA‑CCGGAACTGGATACCCAGCTACGGCCTAACCATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCa                                                                                                                                                                       <  1:398215/239‑1 (MQ=255)
                                                                             cGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GACAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAg                                                                                                                                                                  >  1:370683/1‑239 (MQ=255)
                                                                             cGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGACATGGCACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGG‑GGAAGAAGTGCGTAAGCAGGCAGAAGGAGCAAACGTGCTCTACACCACCATTAAg                                                                                                                                                                  >  2:524993/1‑239 (MQ=255)
                                                                                ctttgtcgcgtgtacccGGAACGGGTTACCCAGCGGGGGACAAAACGTGTGGATGGCAGGGCGCGGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGGGCGGGAAACCTGGCGCATTGGCGACGCCATGGAACAAGTGAGGGAAGGGTTGAATAAGTGGATGCGCGGTGAGCCAGGGCGGGA‑GAAAGAGCGGGGTAAGCTGGAAGATGATAACAACTTTCTCTACACCGCCATTAAGCt                                                                                                                                                                <  2:288023/224‑1 (MQ=255)
                                                                                  attCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTa                                                                                                                                                             >  1:299274/1‑239 (MQ=255)
                                                                                                        gATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTCGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGCCGCCATGGAACAGATGATGTAAGGGTTGAACAAAGTGATGCACGGTAAGCCACGGCAGCA‑AGAAGAGCTGCGCAAGCTGGCAGAAGATAACAACGTTCTCTACCCCGCCATTAAGCTGTAACTGGCGCGGAAGCGaaaaaa                                                                                                                                        >  2:459558/1‑237 (MQ=255)
                                                                                                             ccAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGG‑GAAAGAGCTGCGTAAGCTGGCAGATGAGATCAAAGTTCTCTAAACCGCAATTAAACGGTAT‑TGGGGCggggtgcaaaaaaaaagaa                                                                                                                                  >  2:814828/1‑222 (MQ=255)
                                                                                                             ccAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGGAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGc                                                                                                                                   >  1:125899/1‑239 (MQ=255)
                                                                                                             ccAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGGTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGAGGAAATCAACGTTCTCTAAACCGCCATTAGGCTGTAACTgggcgggagggcaaaaaaaaaga                                                                                                                                   >  2:541189/1‑218 (MQ=255)
                                                                                                                  tACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTacac                                                                                                                                                                            >  2:323491/1‑193 (MQ=255)
                                                                                                                  tACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTacac                                                                                                                                                                            <  1:323491/193‑1 (MQ=255)
                                                                                                                  tACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCg                                                                                                                              >  1:626157/1‑239 (MQ=255)
                                                                                                                         tAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGAAGGAAGGGTTGAATAAAGGACTGCACGGTGAGCCACGGGAGGG‑GAAAGAGCGGCGAAAGCTGTGAGAGGATATACACGTGCTCTCTACCGCCATTAAGTTGTAGCGGGAGGGGCTGGCAAAAAAAGGGCtaggaggggaag                                                                                                                       >  2:682394/1‑228 (MQ=255)
                                                                                                                           aaCATCGGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAAc                                                                                                                                                                                       <  1:405256/173‑1 (MQ=255)
                                                                                                                               tCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTcg                                                                                                                  >  1:150274/1‑238 (MQ=255)
                                                                                                                                 tGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGCATAAAGTGATGCACGGTGAGCCACGGCCGGA‑GACAAAGCTGCGTAAGCTGGCAGATGATGTCAACGTTCCCTAAACCGCCATTAAGCTGAATCTGGCGCGGATGCCCAAATAAAAGCGGGGGGGGGAGGAGTccggg                                                                                                               >  2:204668/1‑235 (MQ=255)
                                                                                                                                 tGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACGCCCATTAAGCTGTATCTGCCCCGGTTGCCAAAAAAAGACCTGGCggaggacagacagcg                                                                                                                >  1:388506/1‑229 (MQ=255)
                                                                                                                                   gATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAg                                                                                                                                                                                                         <  1:557747/147‑1 (MQ=255)
                                                                                                                                   gATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAg                                                                                                                                                                                                         >  2:557747/1‑147 (MQ=255)
                                                                                                                                    gtgtcggggggcgggtcccGCCCACGGTTGC‑CTGGGGAACCGCCGGGCGCAAACCCCGGGGCATGGGCGACGCCAGGGAAGAGATGAGGGAAGGGTTGAATAAAGGGATGCGCGGTGAGCCAGGGCAGGA‑GAAAGAGCTGGGTAAGCTGGAAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGcgccg                                                                                                             <  2:8077/220‑1 (MQ=255)
                                                                                                                                    atgccggcgcgcGGGATCCGCCCCCGTTTGCTCGGGCGAA‑CGCCGCGCGCAAAACCCTGCGCATGGGCGACGCAATGGAACAGATGAGGGAAGGTTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGcgccg                                                                                                             <  1:478835/232‑1 (MQ=255)
                                                                                                                                    agtccagccgcgtGGATGCCCCCACGCTTGCTCGGGGGAA‑CGCCGCGCGGGAACCCCTGCGCATTGCCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGGAATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCTTAAGCTGGAAGATGATATAAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGcgccg                                                                                                             <  1:33735/226‑1 (MQ=255)
                                                                                                                                    aggTCGGCGCGGTGGATACGCCCACGCTCGCTCTGGCGAA‑CGCGGGGGGGGAAACCCGGCGAATTGGCAACGCAATGGAACAGATGATGGAAGGGTTGAATAAAGGGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGGAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGcgccg                                                                                                             <  1:294678/236‑1 (MQ=255)
                                                                                                                                           cgcgcTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGACGCGACGCTGGGCag                                                                                                     >  1:604522/1‑239 (MQ=255)
                                                                                                                                           cgcgcTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCGACACCGCCCTTAAGCTGGATCTGGCGCGGATGCCCAAAAAAGGGCTGGCGGGGGaaaaagaggggcgcggggggg                                                                                                     >  2:478835/1‑220 (MQ=255)
                                                                                                                                            gcgcTGGATACGCCCCCGCTGGCCCGGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGACCAGATGATGGAAGGGTTGAATAAAGTGATGCAGGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCa                                                                                                      <  1:632939/237‑1 (MQ=255)
                                                                                                                                             cgcTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCaga                                                                                                    >  1:8077/1‑238 (MQ=255)
                                                                                                                                                tGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTacac                                                                                                                                                                            <  1:300482/163‑1 (MQ=255)
                                                                                                                                                tGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTacac                                                                                                                                                                            >  2:300482/1‑163 (MQ=255)
                                                                                                                                                        cccACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAAt                                                                                        >  1:86482/1‑239 (MQ=255)
                                                                                                                                                         ccACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAAt                                                                                        >  1:216514/1‑238 (MQ=255)
                                                                                                                                                            cGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTc                                                                                     >  1:840088/1‑238 (MQ=255)
                                                                                                                                                            cGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGAAGAGCTAACCGCAATGTc                                                                                     >  1:282941/1‑238 (MQ=255)
                                                                                                                                                              gtttctgtGGCAAA‑CGCCGCGCGCAAACCCGTGCCGTTGGGCCACGCCATGGAACAGAGTAGGGAGGGGTTGAATAAAGTGATGCACGGTGAGCCAGGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCt                                                                                  <  2:388506/232‑1 (MQ=255)
                                                                                                                                                              cTTTCTCTGCCGAA‑GCCCGCGCGTGAACCCCTGCGATTGGGGGACGCGATGGAACGGATGGAGGAAGGGTGGAATAAAGTGAGGCAGGGTGAGCCACGGCAGGA‑AAAAGACCTGCGTAAGCGGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCt                                                                                  <  2:444733/239‑1 (MQ=255)
                                                                                                                                                               ttgcctgggCAAA‑CGCCGCGCGACAAACCCTGCGAATTGGCGACGGCAGGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCCCGGTGAGCCAGGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCt                                                                                  <  1:164985/231‑1 (MQ=255)
                                                                                                                                                               ttGCTGGGGCGAA‑CGCCGGGCGAGAAACCCGGCGGAGTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGAGGCCCGGTGAGCCAGGGAAGGA‑GAAAGAGCTGGGTAAGCTGGAAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCt                                                                                  <  1:474104/238‑1 (MQ=255)
                                                                                                                                                               ttGCTCGGGCGAA‑CGCCGCGCGCAAACCCCTGCGCATTGGCGACGCTATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCt                                                                                  <  1:294749/238‑1 (MQ=255)
                                                                                                                                                                       gCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGGGGAAACGTAGGGCCGCTGGGCAGGGATCATCGGAATGGCGCCCACC‑CTTGa                                                                        >  1:445583/1‑239 (MQ=255)
                                                                                                                                                                       gCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAAC‑CTTGa                                                                        >  1:199742/1‑239 (MQ=255)
                                                                                                                                                                         aaa‑CGCCCGGCGCAAACCCCTGCGAATGGGCGACGCAAGGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAAC‑CTTGaa                                                                       <  1:320398/237‑1 (MQ=255)
                                                                                                                                                                             cgccgcGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCa                                                                                                                                                                       <  1:438482/140‑1 (MQ=255)
                                                                                                                                                                             cgccgcGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCa                                                                                                                                                                       >  2:438482/1‑140 (MQ=255)
                                                                                                                                                                                cgcgcgcgAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGCGGAAGAGTCGCGCCGCTGGGCAGAGACACTCGAAATGTCGCCCAAC‑CTTGAACAGGcc                                                                 >  1:859521/1‑238 (MQ=255)
                                                                                                                                                                                  cgcgcgAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGCGTCGCGCCGCTGGGCAGAGCTCATCGTAATGTCGCTCAAC‑CTTGAACAGGctacc                                                              >  1:632512/1‑235 (MQ=255)
                                                                                                                                                                                               gcgcATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAAC‑CTTGAACAGGCCTCCGATATCGTCGAg                                                  >  1:772163/1‑238 (MQ=255)
                                                                                                                                                                                               gcgcATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAAC‑CTTGAACAGGCCTCCGATATCGTCGAg                                                  >  1:330523/1‑238 (MQ=255)
                                                                                                                                                                                                 gcATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCCCAAC‑CTTGAAAAGGCCCCCGGTAtcggcaggggc                                               >  2:150709/1‑231 (MQ=255)
                                                                                                                                                                                                     tGGCGACGCCATGGAACAGATGATGGAAGGGTTGCATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGCATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGCGGAAAAGTCGCCACGCTGGCCGGAGAGCATCGAAA‑GCCGCCCCACACTAGAACAGGCCTCCAGTAtcgctaaacccacgg                                           >  2:246431/1‑227 (MQ=255)
                                                                                                                                                                                                          aCGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCCTCGCAATGTCCCTCAAC‑CTTGAACAAGCCTCCGATAACCACCAGCGCATggggcgc                                      >  2:78096/1‑235 (MQ=255)
                                                                                                                                                                                                          aCGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCCCGCCGCTGGGCAAGGATCATCCAAAGGTCGCCCAAC‑CTTGAACAGGCCCCCCGAATCGTCGAgcggcggggccgc                                      >  2:886718/1‑229 (MQ=255)
                                                                                                                                                                                                                        atgatgGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGGAGAGTCGCGCCGCTGGGCAGAGATCATAGAAATGTCGCTCACC‑CTTGCACAGGCCCCCGCACTCGTCCGGGGCATGGGCacgcaaaatgttggaaa                        >  2:175528/1‑223 (MQ=255)
                                                                                                                                                                                                                        atgatgGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCCGAGAACATCGAAATGTCGCCCCAC‑CTTGAACCGCGCTCCGTTATCGTGGAGCGCATGGGCGGCGAAAATGATGACaa                        >  2:641550/1‑239 (MQ=255)
                                                                                                                                                                                                                        atgatgGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGGTATCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGATGGCGGATGCAGAGTCCCGCCGCTGGGCAGAGATCATCGAAATGTCGGCCAAC‑CTTGAACAGGACTCCGAAAACGTCGAGAGCAATGGCaagaaaagtaccaacaa                        >  2:861365/1‑223 (MQ=255)
                                                                                                                                                                                                                          gatgGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCACCGAAATGTCGCTCAAC‑CTTGAACAGGCCTCCGATATCGTCGAGCGCACGGGCACGGAAATTGcgaccaag                       >  1:50746/1‑231 (MQ=255)
                                                                                                                                                                                                                           ggggAGGGTTTGAATAATGTGGTTCAGGGGGGCCCAGGGCAGGA‑AAAAGAGCTGCGGAGGTGGGAAGATGATTACACGGTGCTCAAACCCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAAC‑CTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAAt                      <  2:125899/236‑1 (MQ=255)
                                                                                                                                                                                                                             ggAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAAC‑CTTGAACAGGCCTCCGATCTCGCCGAGGACATGGGAAGCGAAATTGTTAACaaaacca                   >  1:434040/1‑235 (MQ=255)
                                                                                                                                                                                                                                  ggTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGGCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAAC‑CTTGAACAGGCCTCCGACATCGTCGAGCGCAGGGTGAGCGAAATTGCTGACAAAACACggggg              >  2:852206/1‑237 (MQ=255)
                                                                                                                                                                                                                                   gTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAAC‑CTTGAACAGGCCTCCGAAACCGCCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGgca              >  1:658245/1‑238 (MQ=255)
                                                                                                                                                                                                                                       aaaaaaGGGATGGACGGTGAGCCCGGGCAGGG‑AAAAGAGCTGCTTAAGCTGGCAGATGATAACAACGTTCCTTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAAC‑CTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCAcg         <  1:673121/236‑1 (MQ=255)
                                                                                                                                                                                                                                              tGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAAC‑CTTGAACAGGCCCCCGATATCGTCGAGCGCATGGGCAGCCAAATTGCGAAAAAAACACAGGCAACAcggcggcga  >  2:577379/1‑236 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                
TCCACGTCTTCTACAACCTTGTACGTTGCCTGG‑TCATGCTGCCATTTGTTGACCCGATGGCACGGTTTTG‑CAAAACGATTATTCGCGATGAA‑CCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAA‑CGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGA‑GAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAAC‑CTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCA  >  NC_000913/4228562‑4229034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A