Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F9 I0 R1	103	124.7	2465218	97.5%	2403587	230.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	628,868	T→G	27.8%	G106G (GGT→GGG)	entA →	2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	628,868	0	T	G	27.8%	115.8 / 4.4	64	G106G (GGT→GGG)	entA	2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (14/27); new base G (19/2); total (34/30)
Fisher's exact test for biased strand distribution p-value = 2.98e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTGGCGGGTAGCGGCGTGCGCTGTAATGTGGTTTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTG  >  NC_000913/628642‑629105

aaGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATTGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGTGGGTAACGTCGGCGGGGC‑GGTTTAccct                                                                                                                                                                                                                                         >  2:8431/1‑237 (MQ=255)

     aCAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGGCCGATCAGCTCAGTAAAGAGGGCTGGCAGCAGACTTTTGCGGGTAACGTCGGCGGGGC‑GGTTTAACTGT‑Tcc                                                                                                                                                                                                                                    >  1:1151462/1‑238 (MQ=255)

            ttGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGTGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAAcc                                                                                                                                                                                                                           >  2:33815/1‑239 (MQ=255)

                  aaGCGTTCCCTCAGGAGCAATATCCCTTTGCGACCCGAGTGGTGGGTGTTGCCGACGCTGAGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGGAACGGAGGGACTGGGCGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAAc                                                                                                                                                                                                                     <  1:841032/239‑1 (MQ=255)

                   aGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGGGCGACCGATCAGCTCAGTAAAGAGGGCTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGGGC‑GCGTAACCTGT‑TCCAG‑CAAACCATTAAc                                                                                                                                                                                                                     >  1:212128/1‑238 (MQ=255)

                   aGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCCGCTCAGTAAAGAGGACTGGCAGCAGACTTTTTCGGGTAACGTCTGCGGGGC‑GTTTTACCTCT‑TCCCG‑CAAACAATGaaac                                                                                                                                                                                                                    >  1:803061/1‑237 (MQ=255)

                   aGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGGGG‑GGTTAACCTGG‑TCCAG‑CAAAACATGGAcc                                                                                                                                                                                                                    >  1:538480/1‑239 (MQ=255)

                    gCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTTCGGTTAACGTCGGCGGTGC‑GTTTAAACTGT‑TCCAG‑CCAAACATGAACCa                                                                                                                                                                                                                   >  2:989680/1‑239 (MQ=255)

                    gCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCa                                                                                                                                                                                                                   >  2:556964/1‑239 (MQ=255)

                              aGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTTCGGTTAACGTCGGCGGGGG‑GTTTAACCCGT‑TCCCG‑CAAAACATGAACCA‑GTTTTGCCg                                                                                                                                                                                                         >  2:277169/1‑238 (MQ=255)

                              aGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCCCAGGAAAGAGGACAGGCAGCAGACGTTTGCGGTTAACGTCGGCGGGGCGGTTTAACC‑GG‑TCCAA‑CAAACCATGacaaaggttctccg                                                                                                                                                                                                          >  1:784597/1‑225 (MQ=255)

                                 aGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCCCAGTAAAAAAGGCTGGCAGCAGACTTTTGCGGGTAACGGCGGCGGGGC‑GGTTGACCCTT‑TTCCG‑CAAAcccaggccccagttcccccctc                                                                                                                                                                                                       >  1:1124042/1‑218 (MQ=255)

                                   cAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACCCATGGGGCCGGCCGAGCAGGTCAGTGAAGAGGACTGGCGACAGACCTTTGCGGTTAAAGTCGGGGGGGC‑GTTGAACCTGT‑TCCaa‑ccaacccctaacccacttgcccacccacc                                                                                                                                                                                                    >  1:464280/1‑216 (MQ=255)

                                      tatCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCACTGGGGCGACCGATCAGCTTCGTAAAGAGGACTGGCAGCCGACTTTTGCGGTTACAGGCGGGGGGGG‑GGTTTAGCTGTATCCAG‑CAAACAACACAACA‑TTTTCG‑CGTCCg‑cgggg                                                                                                                                                                                               >  1:833563/1‑239 (MQ=255)

                                        tCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAg‑cggggcg                                                                                                                                                                                             <  2:1125617/239‑1 (MQ=255)

                                        tCCCTGTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAg‑cggggcg                                                                                                                                                                                             <  1:255465/239‑1 (MQ=255)

                                             ttGCGACCGAGGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAACCGGAGCGGCGGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGGGTGATCGATCCGCTCAGTAAAGAGGACTGGCAGCGGACCTTTGCGGTTAACGTCGCCCGGGC‑GGTTAACCCGT‑TCCCGCCAACCCTTGAACC‑‑GGTTCGCCGCCCC‑CCgggccgggcg                                                                                                                                                                                        >  1:546850/1‑239 (MQ=255)

                                              tGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGGGG‑GTTTAAACTGT‑TTCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGgggcggggcgg                                                                                                                                                                                       >  2:779810/1‑238 (MQ=255)

                                                  aCCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGGATTTTACGCATGGGCGCGGCCCCTCAGCTCAGTCAAGAGGACCGGCCGCCGGCTTTTGCGGTTTACGTCGGCGGGGC‑GTTTTACCCTT‑TCCCG‑CCAACCACGAACCAGGTTTCCCCG‑CCG‑ccgggcgggggcggtgt                                                                                                                                                                                   >  1:64806/1‑232 (MQ=255)

                                                   ccGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGt                                                                                                                                                                                   <  2:607877/238‑1 (MQ=255)

                                                      aaGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAGCGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGGAAAAAGGACTGGCAGCAGACTTTTGCGGTTAACGTCCGCGGGGG‑GGTTTACCCGT‑TCCAG‑CAAACCATGAACCA‑GTTTTGCCCTCCG‑cgggggggggggggtgtcgcc                                                                                                                                                                               >  1:1110695/1‑228 (MQ=255)

                                                                   tGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGCATTTTACGCCTGGGCGCGACCGATCAGCCCAGTAAAGAGGACTGGCAGCCCACTTTTTCGGTTAACGTCCGCGGTGC‑GTTTTACCTCT‑TCCCC‑CCAACCCTGAACCC‑GTTTCGCCGTCAA‑CGGGGCGGGGGGGCTGTCACTGTGGccttcgcc                                                                                                                                                                   >  1:1027235/1‑231 (MQ=255)

                                                                   tGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCCCAGTAAAGAGGGCTGGCAGCAGACTTTTTCGGGTAACGTCGGCGGGGG‑GTTTAAACCGT‑TCCAG‑CAAAACAATGACCC‑GTTTTCCCGTCAC‑cggggcgggggggttttatactttggcctccga                                                                                                                                                                   >  1:351010/1‑215 (MQ=255)

                                                                                     cgcgCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGt                                                                                                                                                <  2:1125612/238‑1 (MQ=255)

                                                                                        gcAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCCGACTTTTTCGGTTCACGTCGGCGGGGG‑GGTTTACCTGG‑TCACC‑CAAAACCAGAACCA‑CATTTCCCGTCCG‑CGGGGCGGGGCGCATGTCTCTCTGGC‑GCTCGGcgccccccgcccgcccccgcatg                                                                                                                                            >  1:651070/1‑220 (MQ=255)

                                                                                        gcAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTAtt                                                                                                                                             <  1:1189851/238‑1 (MQ=255)

                                                                                        gcAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGGTAACGTCGGCGGGGC‑GTTTAACCTGT‑TCCAG‑CAAAACATGAACCA‑GGTTTGCCCTCAG‑CGGGGCGGGGGGGTTGTTCCTGTGGC‑GTCCGACCCCCCGCACCCGCcccggaatg                                                                                                                                            >  2:39395/1‑234 (MQ=255)

                                                                                          aaGTGTGTCAGCGACTGTTAGCTGAAACGGGGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCATCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGc                                                                                                                                          <  2:546850/239‑1 (MQ=255)

                                                                                             tgtgtCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GGTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGGTTGTTACTGTGGC‑GGCCGGCGCCGCGCACACCCCGCGGATTTGCAtt                                                                                                                                       >  2:793689/1‑238 (MQ=255)

                                                                                             cgtgtCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGAATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GCTTAACCCCG‑TCCAG‑CAAACCATGGAACA‑GTTTCGCCCGCAG‑CGGGGGGGGGCGGGTTTGACTGTGGG‑GCCCGGCGCCCCGcaccaccgccgtgctgggc                                                                                                                                         >  1:605910/2‑221 (MQ=255)

                                                                                              gtgtCAGCGACTGTTAGCTGAAGCGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATg                                                                                                                                       <  2:1110695/238‑1 (MQ=255)

                                                                                              gtgtCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCAt                                                                                                                                        <  2:1151462/237‑1 (MQ=255)

                                                                                              gtgtCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATg                                                                                                                                       <  2:746339/238‑1 (MQ=255)

                                                                                               tgtCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCCGCAGACTTTTGCGGGTAACGTCCGCGGGGC‑GGTTTACCCTT‑TCCCG‑CAAACCATGACACA‑GTTTTGCCCGCCG‑CGGGGGGGGGCGGCTTTTCCTTTGGG‑GCCCCGCGCCCCCCACACGCCGCGTACTgggcgtgg                                                                                                                                     >  1:743143/1‑233 (MQ=255)

                                                                                                gtCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAg                                                                                                                                     <  2:61539/238‑1 (MQ=255)

                                                                                                                                  cgcgCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGc                                                                                                  <  2:1232121/238‑1 (MQ=255)

                                                                                                                                          tCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGtt                                                                                          <  1:556964/238‑1 (MQ=255)

                                                                                                                                                cggcggGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGt                                                                                    <  1:823758/238‑1 (MQ=255)

                                                                                                                                                   cggGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGG‑GGTTAACCTGT‑TCCAG‑CAAAACATGAACCA‑GTTTCGCCGTCAGCCGGGCCGGGGCGATT‑TCACCTTGGCGGTCCGA‑GCCCCCCACCCCCCCCCGACTGGCCCGGGTGCCTTTGGCGGATCGAAAGCGGCGGCTGAAAGGctcgggctgggggtccgg                                                                                 >  1:1031899/1‑222 (MQ=255)

                                                                                                                                                   cggGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCgg                                                                                 <  2:429152/238‑1 (MQ=255)

                                                                                                                                                         ttttACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGaa                                                                          <  2:128138/239‑1 (MQ=255)

                                                                                                                                                            tACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTg                                                                       <  1:33815/239‑1 (MQ=255)

                                                                                                                                                                 aTGGGCGCGACCGATCAGCTCAGTAAAGCGGACTGGCAGCAGACTTTTGCGGCTAACGTCTGCGGGGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGGACCA‑GGTTCGCCGTCAG‑CGGGGCGGGGCGCTTGTCACTGTGGC‑GGCCGGAGCCGCGCACCCGCCGCCGATTGGGATGAGTGCTTATGGGGCCTGGGAAGGGGC‑GCCGACAAAGCTGGCTGTGCGCGGCGCGGCGGAACTgggcgg                                                                  >  1:885983/1‑235 (MQ=255)

                                                                                                                                                                 aTGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTGGCggg                                                                  >  2:582694/1‑239 (MQ=255)

                                                                                                                                                                   gggCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCCCCCCCGCGCCCCAGCCCCGCATTGGCCTGAGTGCCTTTGGCGCCTCGCAACGCGC‑CCTCAAAAGCCTGGCGTTGCGCGTTGGGCCGGGACTGGCCGGtg                                                                >  1:499016/1‑238 (MQ=255)

                                                                                                                                                                           ccGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTGGCGGGTAGCGGCGTg                                                        <  2:99320/239‑1 (MQ=255)

                                                                                                                                                                           ccGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTGGCGGGTAGCGGCGTg                                                        <  2:212128/239‑1 (MQ=255)

                                                                                                                                                                           ccGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTGGCGGGTAGCGGCGTg                                                        <  2:1144763/239‑1 (MQ=255)

                                                                                                                                                                             gATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTGGCGGGTAGCGGCGTgcg                                                      <  2:784597/239‑1 (MQ=255)

                                                                                                                                                                                        tAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCCG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACCCGCCGCGGATTGGCATGAGTGCTTTTGGCGCCTCGGAAGCGGC‑GCGGGAAAGCCCGGCGTTGGGCGTCGGGCTGGGACTGGCgggggggggggggcgggctgaggtgg                                           >  1:908213/1‑216 (MQ=255)

                                                                                                                                                                                             agGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGc                                                                               >  2:501306/1‑198 (MQ=255)

                                                                                                                                                                                             agGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGc                                                                               <  1:501306/198‑1 (MQ=255)

                                                                                                                                                                                             agGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTGGCGGGGTGCGGCGGGGGCCGTCAAGTGGgttt                                       >  2:461220/1‑237 (MQ=255)

                                                                                                                                                                                             agGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGCGC‑GTTTTACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGGGATTGTGACTGCGGC‑GTCCGCCGCCGCGCACACGCCGCGGATTTGCATGAGGGCTTATGGCGCAACGAGAGCGGG‑GCCGCAAAGCCCGGCGCTGGGCGCCGGGCTGGGACCTGCGGGGCgcgggcgggggcgggggtcgggtgt                                       >  1:1037250/1‑217 (MQ=255)

                                                                                                                                                                                                aCTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGGTTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGGATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAACCTGGGGTTGAGCGTCGGGCTGGAACTGGCGGGGAGCGGGGGgcggcgggattggggtttttc                                    >  1:478266/1‑220 (MQ=255)

                                                                                                                                                                                                  tGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGCTTGTCCCCGTGGC‑CTCCGGCGCCGCGCACCCGCCGCGTATTGGCGTGGGTGCTTATGGGGGATCGGAAGCGGC‑GCTCACAAGCCTGGCGCTGCGCGTCGGGCTGGGACTGGCGGGGTGCGGGGcgcgggggggagtggggtttctcgc                                 >  1:340777/1‑218 (MQ=255)

                                                                                                                                                                                                         agtcTTTTGCGGTTAGCGGCGGCGGTGC‑GTTTAGCCTGT‑GCCAG‑CAAACCATGACCCA‑GTTGCGCCGTCAG‑CGGGGCGGGGTGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTGGCGGGTAGCGGCGTGCGCTGTAATGTGGTTTCGCCTGGCTcc                             <  1:8431/233‑1 (MQ=255)

                                                                                                                                                                                                            cTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTGGCGGGTAGCGGCGTGCGCTGTAATGTGGTTTCGCCTGGCTCcaccga                        <  2:538480/238‑1 (MQ=255)

                                                                                                                                                                                                             ttttGCGGTTAACGTCGGCGGGGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTGGCGGGTAGCGGCGTGCGCTGTAATGTGGTTTCGCCTGGCTCcaccgaca                      <  1:779810/239‑1 (MQ=255)

                                                                                                                                                                                                             ttgtGCGGTTAACGGCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTGGCGGGTAGCGGCGTGCGCTGTAATGTGGTTTCGCCTGGCTCcaccgaca                      <  1:39395/236‑1 (MQ=255)

                                                                                                                                                                                                             tgttGCGGTTAACGTCGGCGGGGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTGGCGGGTAGCGGCGTGCGCTGTAATGTGGTTTCGCCTGGCTCcaccgaca                      <  2:885983/237‑1 (MQ=255)

                                                                                                                                                                                                                          gTCGGCGGAGC‑GTATAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTGGCGGGTAGCGGCGTGCGCTGTAATGTGGTTTCGCCTGGCTCCACCGACACCGATATGCAacg         <  2:245435/239‑1 (MQ=255)

                                                                                                                                                                                                                            cggcggTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTGGCGGGTAGCGGCGTGCGCTGTAATGTGGTTTCGCCTGGCTCCACCGACACCGATATGCAacgca       <  2:64806/239‑1 (MQ=255)

                                                                                                                                                                                                                               cggTGC‑GTTTAACCTGT‑TCCAG‑AAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTGGCGGGTAGCGGCGTGCGCTGTAATGTGGTTTCGCCTGGCTCCACCGACACCGATATGCAacgcacg     <  2:383808/238‑1 (MQ=255)

                                                                                                                                                                                                                                  tGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTGGCGGGTAGCGGCGTGCGCTGTAATGTGGTTTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCtg  <  2:651070/238‑1 (MQ=255)

AAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGC‑GTTTAACCTGT‑TCCAG‑CAAACCATGAACCA‑GTTTCGCCGTCAG‑CGGGGCGGGGCGATTGTCACTGTGGC‑GTCCGACGCCGCGCACACGCCGCGTATTGGCATGAGTGCTTATGGCGCATCGAAAGCGGC‑GCTGAAAAGCCTGGCGTTGAGCGTCGGGCTGGAACTGGCGGGTAGCGGCGTGCGCTGTAATGTGGTTTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTG  >  NC_000913/628642‑629105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A