Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F18 I0 R1	274	115.5	2655328	86.7%	2302169	226.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	665,104	A→C	18.9%	S29R (AGT→AGG)	rlpA ←	septal ring protein, suppressor of prc, minor lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	665,104	0	A	C	18.9%	200.0 / 9.8	103	S29R (AGT→AGG)	rlpA	septal ring protein, suppressor of prc, minor lipoprotein
Reads supporting (aligned to +/- strand): ref base A (39/39); new base C (9/14); total (48/55)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

CGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAAT  >  NC_000913/664870‑665332

cgtcggcgtggtggggagaactGCTCCGCCAGGGGCCGTCAGGTTACTGCGGGGTCCGGCATCAAAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTa                                                                                                                                                                                                                                         <  2:462240/218‑1 (MQ=255)

     gCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACCCTTACCg                                                                                                                                                                                                                                      >  2:544262/1‑237 (MQ=255)

         tgtCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGGAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACCCGGGT‑CCGCCCCGCTAATTTCAACTCTAGGGCCGTT‑ACATACCCCAGGCGCCGGGACCCTTACCgcccttg                                                                                                                                                                                                                                >  1:904621/1‑233 (MQ=255)

         tggcGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGTTTCGGCATCATAGATGGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTa                                                                                                                                                                                                                                         <  2:1159192/227‑1 (MQ=255)

           tCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTTGCAACGCGGGT‑GCGCCCCGCCAATTTTAAATATAGGGCCGTTCATATACCAC‑GGCTGCCGTCAAATTACCGTCGT‑TTGc                                                                                                                                                                                                                             >  1:17956/1‑239 (MQ=255)

              gATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTCCCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCTTT‑ACATACCGCCGGCGGCGGGACCATTTCCGGCTG‑TTGCTg                                                                                                                                                                                                                           >  1:1236970/1‑238 (MQ=255)

                tCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGCACACTTACCGTCTG‑TTGCTT‑Ac‑c                                                                                                                                                                                                                      >  2:429270/1‑239 (MQ=255)

                tCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTCCCGTCGCGTTCAGTGGTTCGAAACGCGGGG‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACCAACCGCGAGCCGCGGCACCCTTAcctttcccttaaacac                                                                                                                                                                                                                         >  1:1065077/1‑225 (MQ=255)

                   gtttctcccgcgggggCCGTCGGTTTACTGACGGGTTCGTAGTAATTGTTGGCCGCCAGTCCCGCCTGGATAAATCGAGACGGATCCTGCACCATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑catc                                                                                                                                                                                                                   <  1:1081784/225‑1 (MQ=255)

                   aaTGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTTGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑CAATACCCCGGGCCGGGGGACCCTTACCGCCTT‑TTGccctgctct                                                                                                                                                                                                                     >  1:1159192/1‑231 (MQ=255)

                   aaTGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGGAACGCGGGT‑CCGCCCCGCTAATTTCAAATATAGGGCCGTT‑ACATACCGCGGGCTGCGGGACACTTACCGCCTG‑GTGTTG‑Ac‑cccc                                                                                                                                                                                                                   >  1:567863/1‑236 (MQ=255)

                   aaTGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGCCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑cat                                                                                                                                                                                                                    >  2:1081784/1‑238 (MQ=255)

                    aTGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTCCGGCATTATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑catc                                                                                                                                                                                                                   <  2:927222/238‑1 (MQ=255)

                    aTGCTCCCCCAGGGGCCGTCGGGTTGCTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑catc                                                                                                                                                                                                                   <  2:928016/238‑1 (MQ=255)

                     tGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTACTTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑catca                                                                                                                                                                                                                  >  2:248319/1‑238 (MQ=255)

                        ggggCCAGGGGCCGTCAGGTTACGGCGGGGTTCGGAATAATAGGTTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGc                                                                                                                                                                                                              <  2:895678/236‑1 (MQ=255)

                                      tCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑CCCTACCCCCGGCTGCGGTACACTTACCGCCTG‑TTGCTG‑CC‑CACCATCCCCTTTACATGCcccg                                                                                                                                                                                                >  1:716962/1‑239 (MQ=255)

                                      tCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGTTGCGGTACCCTTACCGTCTG‑TTCCTG‑AC‑CATCATCCCTTGTACATGCcccg                                                                                                                                                                                                >  2:760381/1‑239 (MQ=255)

                                      caggtttcttggcGGGTCCGGTATACTATATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCCGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCcgcg                                                                                                                                                                                                <  2:68968/227‑1 (MQ=255)

                                        cgtttcgtGCGGGGTGCGGCATCATAGTTTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAg                                                                                                                                                                                              <  2:976628/232‑1 (MQ=255)

                                            tACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑GC‑CATCATCGCTTGTACATGCCGCGGGCAt                                                                                                                                                                                           >  2:62008/1‑238 (MQ=255)

                                                   gggTTCGGCATCATAGTTTCCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCc‑g                                                                                                                                                                                  <  2:637769/239‑1 (MQ=255)

                                                    ggTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCCCGAGCATTCCTGCc‑gc                                                                                                                                                                                 >  2:12347/1‑239 (MQ=255)

                                                    ggTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGAAAACTTACCGACTG‑TTGCTG‑AC‑CTTCATCGCTTGTACATTCCGCGAGCATTCCTGCc‑g                                                                                                                                                                                  >  2:919531/1‑238 (MQ=255)

                                                     ttgcGGCATCAGAGGTTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTTCCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCc‑gc                                                                                                                                                                                 <  2:1050789/235‑1 (MQ=255)

                                                      ctccctaataagggctGCCGCTACGCCCGCCTGGCTAAACCTGGTCGGATCCTGCCCGATTTTGTTGTTGTTCGCCTGGAGCTGGTAATCCTGATTTGCCGTTGCGTTAATGGGTTCGAAACGCGGGT‑CCGCCCCGTTTATTTCAACTATAGGGCTGTT‑AAATACCGCAGGCTGCGGTACCCTTGCCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCCCGAGCATTCCTGCc‑gcg                                                                                                                                                                                <  1:871478/223‑1 (MQ=255)

                                                        gtcaatataagtttcccgccATTCCCGCCTTGTTAAATCGAGACGGATCCTGCCCGATTTTGGAGCTTTTACCGTTGGGCTGTTAATCCTCATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGATAATTTCAACTAGAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATg                                                                                                                                                                            <  1:919531/224‑1 (MQ=255)

                                                              cggtggtgcccgccgGCCCCCCCGGGGTAAAGCGGGCCTGACCCTGCACGGTTGTGGTGCTTTACCCGGCGCGCTGGTAATCCTTATTGGCCGTCGCGTTCAGGGGTTCGAAACGCGGGG‑CCGCCCCGCTAATTGCAACTATAGGGCCGTT‑ACATACCGCAGGCGGCGGTACCCTTCCCGTCTG‑TTGCTG‑CC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATc                                                                                                                                                                      <  1:429270/230‑1 (MQ=255)

                                                                     tcccgccAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTCCCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGcc                                                                                                                                                               <  2:716962/237‑1 (MQ=255)

                                                                     tcccccctgccccGCCTGGTAAAAGCGGGCGGGTTCCTGCGCGTTTTTGTAGTTGTTCCCGGCGCGCTGGTAATCCTTATTTGCGGTCGGGTTCAGTGGTTCGAAACGGGGGT‑CCGCCCCGCTAATTCAAACTATAGGGCCGTT‑ACATCCCGAAGGCGGCGGTCCACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACAGGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGcc                                                                                                                                                               <  1:238696/229‑1 (MQ=255)

                                                                     tGCCGCCAGTCCCGCCTGGTAAAATCGAGACGGATCCTGTCCGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCTTGATTGGCCTTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGcc                                                                                                                                                               <  2:1034821/239‑1 (MQ=255)

                                                                     tGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTACTTTCAACTATAGGGCCGTT‑CACTACCGCAGGATGCGGTACCCTTACCGCCGG‑TTTCTG‑AC‑ACGCATCGCTTGTACATGCCCCGCGCAGTCCTGCc‑gcgtcacaaatcccagacc                                                                                                                                                                >  1:822995/1‑223 (MQ=255)

                                                                       ctgccACTCCCGCGTGGTTAAATGGAGCGTGTTCCTGCGCGGTTTGGTAGCTTTTACCGTCGGGCTGGTAACCCTGATTTGCCGTAGCGTTCAGTGGTTCGAAACGCGGGC‑CCGCCCCGCTAATTTCAATTATAGGGCCGTT‑ACATCCGGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCAc                                                                                                                                                             <  1:381789/237‑1 (MQ=255)

                                                                       cctccAGGCCCGCCTGGGTAAATCGGGCCGGATCCTGCCCGATTTTGGAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCAc                                                                                                                                                             <  2:638191/236‑1 (MQ=255)

                                                                       ccgccAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATCGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑TC‑CATCATCCCTTTGCCACGCCCCCAGCATTCCTGCC‑CCC‑TGGCTGAACCCGAGCCAc                                                                                                                                                             >  1:330558/1‑239 (MQ=255)

                                                                        cgccAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCCCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCAc                                                                                                                                                             >  2:193719/1‑238 (MQ=255)

                                                                           cttctcccccTGGTTAATTCGAGTCGGTTCTTGCACGGTTTTGTATTTTTTACCGTCGTCGTGGTAATCCTGTTTGGCCGGCCCGTCCAGGGGTTCGAACCCGGGGC‑CCGCCCCGCTAATTTCAACTATGGGGCCGTT‑ACATACCGCAGGCTGCGGTCCACTTCCCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCt                                                                                                                                                         <  1:76539/231‑1 (MQ=255)

                                                                                   ccTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGCCTGCGGTAAACTTACCGTCTG‑TTGTTG‑AC‑CATCATCCCTTGACCATGCCGCGGGCCTTCCTGCC‑GCG‑GTGCAGCCCCCGAGCCACCCCTTAGGCCtt                                                                                                                                                 >  1:1266896/1‑239 (MQ=255)

                                                                                      ggtcaaaTCGAGCCGGATCCCGCAAGGTTTTGTAGCTTTTACCGGCGCGCTGGTAATCCTGATTTGCCGCCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTgcg                                                                                                                                              <  2:824935/235‑1 (MQ=255)

                                                                                      ggCTAAAGCGAGACGGATCCTGCACGATTTTGTAGCTTTTCCCGGCGCGCTGGTAACCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTgcg                                                                                                                                              <  2:1076455/239‑1 (MQ=255)

                                                                                        cTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTACTCCTGGTCTGCCGACCCGTTAAGTGGTTCGAAACGCGTGT‑CCGCCCCGTTAATTTCAACTATAGGGCCTTT‑ACCACCCCCAGGCGGGCGTACCCTTTCCGTCTG‑TTCCTG‑AC‑CATCTTCGCTTGTCCATTCC‑CGCGCACTCCCGCC‑ACGCATTCAGTTACCCAGCCAATACCTACGAATAgcgc                                                                                                                                             >  1:1017676/1‑238 (MQ=255)

                                                                                                 gaCGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTac                                                                                                                                   >  2:1288876/1‑238 (MQ=255)

                                                                                                 gaCGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTAAATGCCGCGAGCATTCCTGCC‑GCG‑ATGGCGATCCCGAGCCACTGCCTCCCCATTTCGCC‑CCCCTTaca                                                                                                                                  >  1:1003812/1‑239 (MQ=255)

                                                                                                  cgggATCCGGCCCGATTTTGTAGCTTTTCCCGGCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTaca                                                                                                                                  <  2:409409/236‑1 (MQ=255)

                                                                                                  aCGGTTTCTGCCCGTTTTTTGCGCTTTTACCCTCGCGTTGGTAATCCTGATTTGCCGTCGCGGTAGGTGGTCCGAAACGGGGGT‑CCGGCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTaca                                                                                                                                  <  1:193719/238‑1 (MQ=255)

                                                                                                       tCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCCCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCTCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCCCTTACACGCttt                                                                                                                            >  2:184294/1‑239 (MQ=255)

                                                                                                          tGCACGATTTTGCAGCTTTTACCGGCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGACTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAg                                                                                                                                                                                              <  2:239376/173‑1 (MQ=255)

                                                                                                          ggacattttttGTGGCTTTTACCGGCGGGCGGGTAACTCTGTTTTGCGTGCGCGTTCGTGGGTTCAAACCGCGGAC‑CCGACCCGCTACTTCCAACTAGAGGCGCGTC‑ACATACCGAGGGTGGCGGTACCCTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACTGGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCg                                                                                                                         <  1:223313/232‑1 (MQ=255)

                                                                                                           ggccgtttttGTCGCTTTTCCCCGCGGGCTGTTAACCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATCCCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGa                                                                                                                        <  2:256861/233‑1 (MQ=255)

                                                                                                           gcccgtttttGTGCCTTTTACCGTCGCGCTGGTAATCCTGTTTTGCCATCGCGTTCAGTGGTTCGAAACGCGGGT‑CCCCCCCTCTAATTTCAACTATAGGGCCGTT‑ACATTCCGCAGGCTGCGGTACTCTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGa                                                                                                                        <  2:147551/233‑1 (MQ=255)

                                                                                                           gcccGATTTTGTAGCTTTTACCGTCCGGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCAAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGa                                                                                                                        <  2:1130853/236‑1 (MQ=255)

                                                                                                           gccataTTTTGTAGCTTTTCCCGGCCCGCTGGTAACCCTAATTTGCCGTCGCGTTCAGGTTTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCATCTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTT‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGa                                                                                                                        <  2:616455/234‑1 (MQ=255)

                                                                                                                aTTTTGTAGCTTCTACCGTCGCGCTGGTAATCCTGATTTGCCGTCCCGTTCCGTGGTTCGAAACTCGTGT‑CCGCCCCGCTAATTTCAACAATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACCCTTCCATCCTG‑TTTCTT‑AC‑CATCATCCCTTGCACATGCCGCTACCATTCCTTCC‑GCC‑ATGAAGCACCCGAGCCGCTCCTTACACACTGCGCACCCTCATAC‑CGCTTTTctgcaccc                                                                                                                   >  1:182281/1‑232 (MQ=255)

                                                                                                                 ttttGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTCCGCA‑CCTCTTACACGCTTTTCGACAACAt                                                                                                                  >  2:908021/1‑239 (MQ=255)

                                                                                                                 ttttGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTAAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCGGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCCTCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGTACACCCAGTTACCCATGGGGCC‑CCACTTACACGCTTTTCGCCAACAt                                                                                                                  >  1:616455/1‑239 (MQ=255)

                                                                                                                    tGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCTAGCCACTGCTTACGCATTGCGCA‑CCTTTTACACGCTTTTCGCCAACActt                                                                                                                >  2:659927/1‑238 (MQ=255)

                                                                                                                        gCTTTTACCGTCGCGCTGGAAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCtg                                                                                                           <  2:904621/239‑1 (MQ=255)

                                                                                                                           tttACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACCTTTTCCTTTgg                                                                                                        >  2:1284742/1‑239 (MQ=255)

                                                                                                                           tttACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTgg                                                                                                        >  2:1168679/1‑239 (MQ=255)

                                                                                                                           tttACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGACTG‑TTGCTG‑AC‑CACCATCCCTTGTACATGCCTCTAGCATCCCTGCC‑GCG‑ATGCAGATCCCGCCCCACTGCTTACGCATTGCGCA‑CACCCTCCACGCTTTTCGACAACATTTACCTCTgg                                                                                                        >  1:231554/1‑239 (MQ=255)

                                                                                                                             tACCGTCGCGCTGGTAATCCTGATTTGCCGGCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTCCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑gt                                                                                                     <  2:567863/239‑1 (MQ=255)

                                                                                                                                   cgcgCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACCTTTTCCTGTGG‑GTGTGTAt                                                                                               >  2:844272/1‑239 (MQ=255)

                                                                                                                                   cgcgCTGGTAAGCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGTTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGAt                                                                                               <  2:1236970/239‑1 (MQ=255)

                                                                                                                                       cTGGTAATCCTGATTGGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGa                                                                                            <  2:291610/238‑1 (MQ=255)

                                                                                                                                           gattctttttttGCCGTCGTGTTCGGGGGTTCGCACCCGGGGTCCCGCCCCG‑TATTTTCAAATATGGGGGCTTT‑ACATACCGAGGGCTGGGGTACACTTCCCGTCTG‑TTGCTG‑AC‑CACCAGCGCTTGTCCATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTa                                                                                       <  1:12347/230‑1 (MQ=255)

                                                                                                                                             aTCCTGATTTGCCGTCGCTTTCAGTGGTTCAAAACGCGGGC‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTCCCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACa                                                                                     <  2:822995/239‑1 (MQ=255)

                                                                                                                                                  gATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACCCTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATccc                                                                                <  2:1003812/239‑1 (MQ=255)

                                                                                                                                                    tttGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTTTGG‑GTGTGGATTGACATTACAATCCCg                                                                               >  2:27745/1‑238 (MQ=255)

                                                                                                                                                    tttGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCCG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑GTGCCGATCCCGAGCCACTGCTTCCGCATTGCTCC‑CCTCTCACACGCTTTTCGACAACCTTTTCCTGTGG‑GGGGGGATTGAAATTACaactccgc                                                                              >  1:786786/1‑233 (MQ=255)

                                                                                                                                                        ccGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GGGTGGATTGACATTACAATCCCGAAccc                                                                          >  2:1158487/1‑239 (MQ=255)

                                                                                                                                                             gcgTTCAGGGGTTCGAACCGCGTGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATCCCCCAGGCTGCGGTACCCTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCa                                                                     <  2:1266896/239‑1 (MQ=255)

                                                                                                                                                               tttgtttaagagacaccgcgGGT‑CCACCCACCAAATTTCAACTATGGGCTCGTG‑ACAGACCGCAGGCTGCGGTACCCTATCCGTCTT‑GTGCGT‑CC‑CATCCTCGCTGTTACGTGCTGCGAGCTTTCCCGCC‑GGG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCGCTTACACGCTTGTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCAt                                                                    <  1:45287/222‑1 (MQ=255)

                                                                                                                                                                  cggTGCTTAGAACCGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACAGACCGCAGGCGGCGGTACCCTTACCGTCTG‑GTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGc                                                                <  2:17956/237‑1 (MQ=255)

                                                                                                                                                                        ttCGAAACGGGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAAt                                                           <  2:912317/238‑1 (MQ=255)

                                                                                                                                                                          cGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGAc                                                                                           >  2:192145/1‑204 (MQ=255)

                                                                                                                                                                                tggggT‑CCGACCCGCTAATTTCAACTATGGGGCCGTT‑ACATACCGCAGGCTGCGGTCCCCTTACCGTCCG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGa                                                  <  2:1279360/237‑1 (MQ=255)

                                                                                                                                                                                gggggT‑CCCGCTCGCTTTTTTCCACTATTGGGCGTTT‑CCACACCGCGGGCCGCGTTCCACTTCCCGCCTG‑TTGGTG‑AC‑CATCCCCGCTTTAACATGCCGGGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGa                                                  <  1:803668/237‑1 (MQ=255)

                                                                                                                                                                                gggggT‑CCCCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATGCCGCAGGCTGCGGTACCCTTCCCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGa                                                  <  2:1038042/237‑1 (MQ=255)

                                                                                                                                                                                cggggT‑CCCCCCCGGTATTTTCACCTTTACGGCCGTT‑ACACACCGCGGCCTGCGGTACACTTACCGTCGG‑TTTCGC‑ACACCTC‑TCGCTTGTACATGCGCGGAGCATTCCTGCC‑GCG‑ACGCCGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGa                                                  <  1:34579/237‑1 (MQ=255)

                                                                                                                                                                                 gcggtgccGCCCCGTTAATTTCACTTATAGGGGCGTT‑ACATACCGGAGGCTGCGGTACATTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACCGGCCGGGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGa                                                  <  1:354453/233‑1 (MQ=255)

                                                                                                                                                                                       ccGCCCCGTTAATTCAAACTATAGGGCCGTT‑TCATCCCCCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATCCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGAtcctc                                             <  2:231554/238‑1 (MQ=255)

                                                                                                                                                                                       ccGCCCCGCTAATTTCAACTATGGGGCCGTG‑ACATTCCGCAGGCTGCGGTACCCTTACCGTCTG‑TTGCTG‑GC‑CATCATCGGTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGAtcctc                                             <  2:380732/238‑1 (MQ=255)

                                                                                                                                                                                        cccccccctattttacattttggggCCTTT‑ACATCCCGCGGGGCGGGGTCACCTTCCCGTTTG‑TTGGTG‑CC‑CATCACCGCTTGTACAGTCCCCGAGCTTCCCGGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCa                                           <  1:1284742/217‑1 (MQ=255)

                                                                                                                                                                                        cGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGCCAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCa                                           >  2:394188/1‑239 (MQ=255)

                                                                                                                                                                                           cccTCTTATTTCCCCTTTAGGGCCGTT‑CCCTACCGCCGGCTGCGGTAC‑CTTTCTCTGTGTTTGGTGCAC‑CA‑CACCGCTTGTACATCCCCGGAGCCTTCCTCCCTGAG‑TTG‑AGATCCCGAGCCACTGCTTACGGATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATaa                                        <  1:601382/239‑1 (MQ=255)

                                                                                                                                                                                           cccGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCCGCACAATTactgtctcttataacca                                         >  2:45287/1‑222 (MQ=255)

                                                                                                                                                                                             cGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTTCTTACGCATTGCGAA‑CCCCTTACACGCTTTTCGACAACCCTTTCCTGTTG‑GTGTGGATTGCAATTAAACTCCCCGAACCACCCCTCACCCAAATTATCCCCGATCCTCCaaaaat                                      >  1:1268041/1‑237 (MQ=255)

                                                                                                                                                                                                            taGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTTCGGATTTACATTACAATCCCGAACCCCGCCACTCCCACAATTACGCCCGATCCCCCAAAAcataaccagggcgaccg                       >  1:527124/1‑223 (MQ=255)

                                                                                                                                                                                                            taGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGa                                                  <  2:697672/212‑1 (MQ=255)

                                                                                                                                                                                                            taGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGa                                                  >  1:697672/1‑212 (MQ=255)

                                                                                                                                                                                                                    tt‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCCGAGGGACCGCAACCCCTa               >  2:562208/1‑239 (MQ=255)

                                                                                                                                                                                                                    tt‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCtc               >  2:275783/1‑238 (MQ=255)

                                                                                                                                                                                                                              cccggCCGCGGTCCCCTTCCCGTCTG‑TTGCTC‑CC‑CATCTTCGCTTTCACTGGCCGCGAGCTTCCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCa      <  1:1158487/236‑1 (MQ=255)

                                                                                                                                                                                                                               cAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTCCAACCGGCAg     >  2:922348/1‑239 (MQ=255)

                                                                                                                                                                                                                               cAGGCTGCGGGACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAAAATTTTCCTGTGGTGAGTGG‑TTGCCATTACAATCCCACACCCACCCATCAGCACACTTAGCGCCGACCCCCCCAAACTCACCCGTGGGACCGGAAcacaacaaaacggcca     >  1:1082789/1‑224 (MQ=255)

                                                                                                                                                                                                                                aGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGACCGGAACCCCTACAACCGGCaaa    >  2:597282/1‑237 (MQ=255)

                                                                                                                                                                                                                                 ggCTGCGGTACCTTTACCGTCTG‑TTGCCG‑AC‑CAGCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGaa   <  2:973055/239‑1 (MQ=255)

                                                                                                                                                                                                                                  gCTGCGGTACCCTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGc                                                                <  2:960199/177‑1 (MQ=255)

                                                                                                                                                                                                                                  gCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGc                                                                >  1:960199/1‑177 (MQ=255)

                                                                                                                                                                                                                                  gCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAAt  >  2:155663/1‑239 (MQ=255)

CGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGT‑CCGCCCCGCTAATTTCAACTATAGGGCCGTT‑ACATACCGCAGGCTGCGGTACACTTACCGTCTG‑TTGCTG‑AC‑CATCATCGCTTGTACATGCCGCGAGCATTCCTGCC‑GCG‑ATGCAGATCCCGAGCCACTGCTTACGCATTGCGCA‑CCTCTTACACGCTTTTCGACAACATTTTCCTGTGG‑GTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAAT  >  NC_000913/664870‑665332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A