Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F18 I0 R1	274	115.5	2655328	86.7%	2302169	226.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	909,181	A→G	13.9%	A38A (GCT→GCC)	ltaE ←	L‑allo‑threonine aldolase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	909,181	0	A	G	13.9%	191.4 / 2.9	94	A38A (GCT→GCC)	ltaE	L‑allo‑threonine aldolase, PLP‑dependent
Reads supporting (aligned to +/- strand): ref base A (41/35); new base G (3/12); total (44/50)
Fisher's exact test for biased strand distribution p-value = 2.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAA  >  NC_000913/908943‑909415

tCGCCATTTTATCCAGGGGTAGCGGGCCGTCGGCCGCCGCGTCTATGGGTTGCGGTTGAATCCTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCaga                                                                                                                                                                                                                                                 <  2:365336/239‑1 (MQ=255)

         tacccAGCGGTAGCGTGCCGTCGGCAGCCGCTTCTATGGGTTGCGGTTGAATCCTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACg                                                                                                                                                                                                                                       <  2:1177956/236‑1 (MQ=255)

              aGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCCGCGCAGGCCCGCAGAGCATTC‑ACGGTAgg                                                                                                                                                                                                                                  >  1:1290367/1‑239 (MQ=255)

                cGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGAAggg                                                                                                                                                                                                                                 >  2:846310/1‑238 (MQ=255)

                cGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑GCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTCCATAGCCTTA‑ACGGTAtgg                                                                                                                                                                                                                                 >  2:482623/1‑238 (MQ=255)

                    aGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGgtcgt                                                                                                                                                                                                                             <  2:310660/238‑1 (MQ=255)

                          ccGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGGTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCCCGTTCGCAGGGACTGGGCATAGCGACCAGGGTGGCCTGAGGGCCGGTCGGCAGAAAAATGGCGGCT‑GCTTTACCGGGA‑A‑ACTGCTCGGCGGAATACTGCGGAGCATTA‑AAAGTAGGGGCGTCCCCgga                                                                                                                                                                                                                      >  1:979980/1‑237 (MQ=255)

                               ggCAGCCGCGTCTAGGGGTTGCGGTTGAATCCTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAAcgt                                                                                                                                                                                                                 <  2:1271404/239‑1 (MQ=255)

                                             aagagaccGGTTGAATCCTGCCCAGGCCCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCggg                                                                                                                                                                                                   <  2:183095/232‑1 (MQ=255)

                                                          aaTACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGCTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑C‑GACTTGCTGCGTAGTCCTGCCGAGCCGTA‑CCGGGCGGGCCGTCCCCGCAAACGCCGCCCCCAACCGGGGCGGcccccctc                                                                                                                                                                                       >  1:944969/1‑232 (MQ=255)

                                                                 cccAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCCGAGCGACCACGTTGGCCTGAGTGCCGGTAGGCAGAAGACTGGCGGCT‑TCTTTACCGGAA‑ATGTTCTCTTGGGCA‑ACCTGCAAAGCATTA‑CCAGTAGGGCCCGCTCCGTAAACGTCGCTCCCCCCCGGGGGGGCcacctctcaccccg                                                                                                                                                                                >  1:946728/1‑226 (MQ=255)

                                                                 cccAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGTAGGGCATTT‑ACGGTGAGGCCGGCTCCCTACAAGTGATCCCCAAACGGGGGGGAcacaatccgtttctt                                                                                                                                                                               >  1:183095/1‑226 (MQ=255)

                                                                 cccAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGa                                                                                                                                                                                >  2:808679/1‑238 (MQ=255)

                                                                 cccAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGGCGTCTCCGTAAACGTCGTTCCCAACCGCGGCGGCCATCATCCGTTCggg                                                                                                                                                                               >  2:1294553/1‑237 (MQ=255)

                                                                 cccAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGGCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAg                                                                                                                                                                               >  2:29171/1‑239 (MQ=255)

                                                                 cccAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGAATTA‑ACCGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCCTCACCGCTTCggc                                                                                                                                                                               >  2:895268/1‑237 (MQ=255)

                                                                 cccAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGCTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTTCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACCTCGTCCCCAACCGGGGCGGCCATCCTCGCTTCGa                                                                                                                                                                                >  2:124550/1‑238 (MQ=255)

                                                                      cACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCC‑TCTTTACCGGAACA‑GTTC‑GCTGCGGGGGCCAGCAGGGACTTA‑ACGGGAGAGGTGTACCCGCAAACGTCGTCCCCAACCGGGGGGGCCGCCATCCCCTCggagaggg                                                                                                                                                                          >  1:491670/1‑232 (MQ=255)

                                                                           ccgcGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GGCTTGCTTAGTCGTCCCGCACAGCCTTA‑ACGGAAGGGGTGTCCCCGTAAACGCATTCCCCAAACGGGGGGGCcacaaacgcgctcggacggggaggg                                                                                                                                                                     >  1:648363/1‑216 (MQ=255)

                                                                           ccgcGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGggcg                                                                                                                                                                     >  2:1201433/1‑238 (MQ=255)

                                                                           ccgcGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGGCCTGCAAGGCATTA‑ACGGTAGGGTCGTTTCCGTAAACGGCGTCCCCAACCCGGGCGGCCATCATCGCTTCGAG‑CATGGcccg                                                                                                                                                                     >  2:867370/1‑238 (MQ=255)

                                                                           ccgcGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGACT‑TATTTATCGGGA‑A‑GCTCTGCTGCGTAGGCCTTCAGAGAATTA‑ACGGTAGGGTCGTCTCCGTAACCGGCGTCCCCAACCGGGGCGGGCATCAACCCTT‑GAGTCATGGcgcg                                                                                                                                                                     >  1:244342/1‑238 (MQ=255)

                                                                                ccACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCCTCATCGCTTCGGG‑CATGGCGCGGCTTgg                                                                                                                                                               >  2:351709/1‑239 (MQ=255)

                                                                                 cACCGGCTTCAAACAGATAGTTACGCGCGGCCTGACCGACAATATACCCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGGGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTgg                                                                                                                                                               <  2:742001/238‑1 (MQ=255)

                                                                                      gttgcAAACAGTTTGTTAGGGGCGGCCTGGCCGACAATGTCCCCGTC‑GCCGCGGTCGCAGTGACTGGGCAGGGCGACCAGGTTGGCCTGAGTGCCGGGAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGt                                                                                                                                                         <  1:29171/235‑1 (MQ=255)

                                                                                               tggtggtttggggcgGCCGGACCGA‑AATATTACCTTGGGCCGCGTTCGCGGTGACTGAGCAAGGGGTCCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATc                                                                                                                                                <  1:595113/227‑1 (MQ=255)

                                                                                                       aTGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGAAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGCCGGCCATCATCGCTTCTGG‑CATGGCGCGGCTTCGCCGGGGAACCGTCTCACTGctgc                                                                                                                                        >  2:865497/1‑236 (MQ=255)

                                                                                                        tGCGCGGCATGGCCGAAAATAGAACTTTC‑GCCGGGTTCGGATGGCCTTGGCAGGGGGACCAGGTTGGCCTGAGTGGCGGTAGGAAGAAAAAGGGCGGCT‑CCTTTACCGGAA‑A‑GCTCTGATGCCTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTaa                                                                                                                                       <  1:839692/239‑1 (MQ=255)

                                                                                                           gcgGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCCTCATCGCTTCGAG‑CATGGCGCGGCTTGGGCGGGGAACGGGCTCACTGCGTaaa                                                                                                                                      >  2:595113/1‑237 (MQ=255)

                                                                                                           gcgGCCTGACCGACAATATACTCTTC‑CCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGCA‑A‑GCTCTGCTGCGTAGTCCTGCCGAGCAGTA‑CCGGTAGCGTCGGCCCCGGAAACGCCGTCCGCGACCGGGGGGGCCATCATCTCTCGCAG‑CACGGCCGGGCCTCGTCGGGGGACGGtgcccactggaaaagc                                                                                                                                    >  1:626958/1‑224 (MQ=255)

                                                                                                           gcgGACTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAaat                                                                                                                                     <  2:1241425/238‑1 (MQ=255)

                                                                                                             ggCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑CCGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGGAACGGTATCACTGCGGAaatcaa                                                                                                                                  >  2:219839/1‑239 (MQ=255)

                                                                                                               ccTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGGAACGGGATCACTGCGTAaataaatc                                                                                                                                >  2:408909/1‑239 (MQ=255)

                                                                                                                cTGACCGAAAATTTACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAaatcaatc                                                                                                                                <  2:16556/238‑1 (MQ=255)

                                                                                                                    ccGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGGCCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATgg                                                                                                                            <  2:244342/238‑1 (MQ=255)

                                                                                                                     cgaacatgtgcctttC‑GCCGCGTTCGCAGTGACTGAACAGGGCGCCCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCGGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCa                                                                                                                          <  2:525562/226‑1 (MQ=255)

                                                                                                                     cgaacatatGCTCTTC‑GCCGCGTTCGCAGGGACTGAGCAGAGCGACCATGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCCCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGACATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCa                                                                                                                          <  2:984687/234‑1 (MQ=255)

                                                                                                                     cGACAATATACCCTTC‑GCCGGGTTGGCAGTGACTGAGCCGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCa                                                                                                                          <  2:201122/239‑1 (MQ=255)

                                                                                                                     cGACAATATACCCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCa                                                                                                                          <  2:420921/239‑1 (MQ=255)

                                                                                                                     cGAAAATATTCTCTTC‑GCCGCGTTCGCAGTGACTGGGCAGGGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCTATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCa                                                                                                                          <  2:491670/239‑1 (MQ=255)

                                                                                                                       aCAATATACTCCTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGGG‑CATGGCGCGGCTTTGTCGGGGAACGGTATCACTGCGTAAATAAATCATGGCATg                                                                                                                        >  2:335772/1‑239 (MQ=255)

                                                                                                                        cAATATCCTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCAt                                                                                                                         <  2:166993/237‑1 (MQ=255)

                                                                                                                        cAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGc                                                                                                                           <  2:778101/235‑1 (MQ=255)

                                                                                                                         aaTATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATAACTGCGTAAATCAATCATGGCATG‑Tc                                                                                                                     <  2:888476/239‑1 (MQ=255)

                                                                                                                                  ttC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTCCGAG‑CATGGCGCGGCCTGGTCGGGGAACGGGTAAACTGCCGAAATCAATCAGGGCATGCTCCtttttg                                                                                                              >  1:888476/1‑237 (MQ=255)

                                                                                                                                  ttC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGGCCCCAACCGGGGCGGCCCTCATCGCTTCGGG‑CATGGCGCGGCTTGGGCGGGGAACGGGATCACCGCCGCAATCAAAAATgggcagggcctattttg                                                                                                             >  1:778101/1‑224 (MQ=255)

                                                                                                                                  ttC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGCCGTCCCCAACCGGGGCGGCCATCATCGCTTCGCG‑CATGGCGCGGCCTGGTCGGGGTACGGGATAACTGCGGAAATCCACAATGGCGCT‑TCCTtattatt                                                                                                            >  1:166993/1‑238 (MQ=255)

                                                                                                                                  ttC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GATCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCCTCATCGCTTCGGG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATAAATCATGGCATG‑ACCTTATTATg                                                                                                            >  2:825855/1‑239 (MQ=255)

                                                                                                                                   tC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGa                                                                                                           >  2:200960/1‑239 (MQ=255)

                                                                                                                                    c‑gccgcGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGGATCACTGCGTAAATCAATCATGGCAGG‑TCCTTATTATGAc                                                                                                          >  2:721596/1‑239 (MQ=255)

                                                                                                                                        cgggTTGGCAGTGACTGGTCAGGGCGACCAGGTGGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGGCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACggg                                                                                                       <  1:1201433/236‑1 (MQ=255)

                                                                                                                                                 ggggagcgaggcgggCGACCGGGTTGGCCTGAGGTGCGGGAGGCAGAAAAAGGGGCGCT‑TCTTTACCGGAA‑A‑GCTCGTGTGCGTAGTCCTGGAGGGCAGTA‑ACGGTAGGGTCGTCGCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑Ca                                                                                              <  1:1243560/224‑1 (MQ=255)

                                                                                                                                                      tgtggcgagcgcccccggTTGGCCTTGGTCCCGGTAGGCCGGAAAATGGCGGTT‑TCTTTGCCGTAA‑A‑GCTCTGCGTCGTTGGCCTGCAGTGAATTA‑ACGGTAGGGGCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCttt                                                                                        <  1:867370/223‑1 (MQ=255)

                                                                                                                                                         aGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGGG‑CATGGCGCGGGTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCCTG‑TCCTTATTTTGACGGGAACTGC‑CACCCctttt                                                                                      >  2:605429/1‑238 (MQ=255)

                                                                                                                                                              agCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGAATTA‑ACGGTAGGGTCGTCTCCGTAAACGGCGTCCCCAACCCGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCCTGGTCGGGGAACGGTGTCACACGCTAACTCCATCATGGCATG‑TCCTTATCTTGACGGGCGATGC‑CACCCCTTTTAc                                                                                    >  1:1039398/1‑235 (MQ=255)

                                                                                                                                                                    tatgTTGGCCTGAGGGGCGGTGGGTAGAAAAATTGTGGTG‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTGGTCCTGAAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCTCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAgt                                                                         <  1:1128057/236‑1 (MQ=255)

                                                                                                                                                                               tggggCCGGTGGGCATAAAAGTGGCGGCT‑TCTTTGCCGTAA‑A‑GCCTTGTTGCGTGTGCCTGCAGGGCATTA‑ACGGAAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCAGCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGcc                                                              <  1:167372/235‑1 (MQ=255)

                                                                                                                                                                                aGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGgc                                                                                                                                                                        >  2:1062335/1‑135 (MQ=255)

                                                                                                                                                                                aGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGgc                                                                                                                                                                        <  1:1062335/135‑1 (MQ=255)

                                                                                                                                                                                aGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCCATCATGGCATG‑ACCTTCTTATTACGGGGAATTG‑CACCCTTTTTAACTT‑AACCAGTTTGTTTTTGc                                                               >  2:1128057/1‑237 (MQ=255)

                                                                                                                                                                                   gCCGGGGGGGAGAAAAGTGG‑GGCTGTTTTTCCCTGAA‑C‑GCTTTGCTGCGTGGGCCCGGAGACGATTA‑ACGGTTGGGTCGTCTCCGTAAACGTTGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGt                                                           <  1:904505/238‑1 (MQ=255)

                                                                                                                                                                                       gtgggCCGAAAAATGGGGGTT‑TCTTTTCCGGAA‑A‑TCCCGGGTGGGTCGTCCCGACGCGCATTA‑ACGGTAGGGTCGGCCCCGTAAACGGCCTCCCCAACCGGGGCGGCCATCCTCCGTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGAt                                                      <  1:1294553/236‑1 (MQ=255)

                                                                                                                                                                                        tAGGGAGAAAAATGGCGGCT‑TCCTTACCGGAA‑A‑GCTGCGCCGCGTAGTGCTGAAGAGGATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGAt                                                      <  2:946728/238‑1 (MQ=255)

                                                                                                                                                                                        tAGGCATGAAAATGGCGGGT‑TTCTTACCGGAA‑C‑GCTCTGCTGCGTAGTCCTGCAGAGCTTTA‑ACGGTATGGTCGTCTCCGTAAACGTCCTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGAt                                                      <  2:649434/238‑1 (MQ=255)

                                                                                                                                                                                         aGGCGGAAAAACGGCGGTT‑TTGTTACCGACA‑C‑TGTCTGCTGCGTATGCCTGAAGGGCATTA‑ACGGGAGGGTCGTCTCCGTAACCGTCGTCCCCAACAGGGGCGGCCATCATCGCTGCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCa                                                    <  1:1310624/239‑1 (MQ=255)

                                                                                                                                                                                          ggCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCCACCGGGTCGGCCATCATCGCTTGGAG‑CATGGCGCGGCTTGGTCGGGGACCGGGATCACACCGGTAATACACCACTGCATG‑TACTTATTTGGACGGGAGATTG‑CCCCCCTTTTACCTTCACCCAGTTTTTTTTcccagagccaga                                                      >  1:1063272/1‑226 (MQ=255)

                                                                                                                                                                                          ggCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGt                                                                                                                                                              <  1:123747/135‑1 (MQ=255)

                                                                                                                                                                                          ggCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGt                                                                                                                                                              >  2:123747/1‑135 (MQ=255)

                                                                                                                                                                                          ggCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCCCTGCGTAAATAAATCATgg                                                                                                                            >  2:801135/1‑169 (MQ=255)

                                                                                                                                                                                          ggCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCCTAAATCAATCATGGCCGG‑GCCTTATTTTTGCGGGGAAAGCACCCCCTTTTACCCTT‑ACCCAG‑TTGTTTTTGCCaagtgggacac                                                   >  1:1054167/1‑229 (MQ=255)

                                                                                                                                                                                               aaaaaTGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGa                                                                                                           <  2:1014207/180‑1 (MQ=255)

                                                                                                                                                                                                      gcggcT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGGCCTGCAGGGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACcgc                                        <  2:1039398/238‑1 (MQ=255)

                                                                                                                                                                                                       cggcT‑TCTGTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CTCCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACcgc                                        <  2:834060/237‑1 (MQ=255)

                                                                                                                                                                                                          tt‑tcttTCCCGGAA‑A‑TCTTTTCTGCGTAGTCCTGCAGGGCATTA‑ACGGTAGGGTCGGTGCCGTAAACGGCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTcc                                    <  1:351709/237‑1 (MQ=255)

                                                                                                                                                                                                          ct‑tcttTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTcc                                    <  2:213481/238‑1 (MQ=255)

                                                                                                                                                                                                                        a‑a‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCCCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTgcgc                      <  2:26493/239‑1 (MQ=255)

                                                                                                                                                                                                                        a‑a‑GCTCTGCTGCGTAGGCCTGCAGGGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCATTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTgcgc                      <  2:1290367/239‑1 (MQ=255)

                                                                                                                                                                                                                          atgCTCTGCCGCGGTGTCCCGCAGGGCTTTACACGTTA‑GGACGTCTGCGTAAACGTGCTCCCCAACCGGGGCGGCCATCCTCGCTTCGAG‑CATGGCGGGGCTTGGTCGGGTAACGGTATCACGGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CATCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTgcgc                      <  1:482623/237‑1 (MQ=255)

                                                                                                                                                                                                                          agctcgtcgcggcttcgcccggagagGAGTA‑AAGGGAGGGTCGTCTCCTGTAACGGCGGCCCCAACCGGGGCGGCCATCATCGCGTCGGG‑CAGGGCTCGGCTTGGTCAGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTgcgc                      <  1:335772/217‑1 (MQ=255)

                                                                                                                                                                                                                          aatctctGCTGCTGAGCCCTGCAGGGCATTA‑ACGGTAGGTTCGTCCCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTgcgc                      <  2:483050/236‑1 (MQ=255)

                                                                                                                                                                                                                          a‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCCCTGCGTAAATCAATCATGGCATG‑TCCTTCTGATGCCGGGAAAAGC‑CGCCCTTTTTACCTT‑TCACGGTTTGTTTTTTCCCCTTCGATCGCTCCCTCACcgctgccccggcggtgtgccgc                     >  1:289589/1‑220 (MQ=255)

                                                                                                                                                                                                                          a‑GCCCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTgcgc                      <  2:380338/238‑1 (MQ=255)

                                                                                                                                                                                                                           agcttcgccgggtcggccTGCAGCGCATTT‑ACTGTAGGGTCGTCTCCGGAAACGTGTCCCCCAACCGGGGCCGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACGGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTgcgc                      <  1:605429/222‑1 (MQ=255)

                                                                                                                                                                                                                           acctctGCCTCGTCGTCCTGCAGAGCATTG‑ACGTTAGGGTCGTTTCCGTAAGCGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGGGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTgcgc                      <  1:895268/236‑1 (MQ=255)

                                                                                                                                                                                                                            actcttgttcGTTGTCCTGGAGGGGATTA‑ACGGGAGGGTCGTTGCCGGAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGGGCGGTTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTgcgcgc                    <  1:808679/230‑1 (MQ=255)

                                                                                                                                                                                                                             ctctGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGCTTGTTTTCGCCCGTTCGATCACTTCATCACCTCGTCCCCCTATGATTGCGCgca                   >  2:849134/1‑239 (MQ=255)

                                                                                                                                                                                                                              tctGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTgcgcgc                    <  2:1054167/237‑1 (MQ=255)

                                                                                                                                                                                                                              tctGCTGCGTAGTCCCGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCgca                   <  2:648363/238‑1 (MQ=255)

                                                                                                                                                                                                                                    tcGTTGTCCTGCAAAGCATTA‑ACGGTAGGGTCGTCTCCGGAAACGCCGTCCCCAACCGGGGCGGCCAGCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCAtata            <  2:708076/238‑1 (MQ=255)

                                                                                                                                                                                                                                    gcgttttCCTGCAGGGCTTTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCAtata            <  1:721596/233‑1 (MQ=255)

                                                                                                                                                                                                                                    gcGTTGGCCGGCAGAGCATTA‑ACGGTAGGGTCGTCCCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCAtata            <  2:1145622/239‑1 (MQ=255)

                                                                                                                                                                                                                                       tAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGGGCGGCATATAAAg         >  2:301316/1‑239 (MQ=255)

                                                                                                                                                                                                                                          tCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCCCCGCGTCCGCTGATGATTGTGCCCAGCAATTACAg         >  2:866057/1‑236 (MQ=255)

                                                                                                                                                                                                                                            cTGCAGGGCATTA‑ACGGTTGGGTCGTCTCCGTAAACGGCGGCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGa    <  2:979438/239‑1 (MQ=255)

                                                                                                                                                                                                                                            cTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGGATCACTGCGTAAAGCAATCATGGCACG‑TCCTTATTATTACGGGAAATGC‑CACCCTTTTTACCTT‑AGACAGTTTGTTTTTGCCAATTCGATACATTCCTCACCCCTTCCCCCGATGATTggcggcagccatacaaggtgga    >  1:611883/1‑218 (MQ=255)

                                                                                                                                                                                                                                               cAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGGCCGCAGCATATACAGGCTGaaa  >  2:811633/1‑238 (MQ=255)

TCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTC‑GCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCT‑TCTTTACCGGAA‑A‑GCTCTGCTGCGTAGTCCTGCAGAGCATTA‑ACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAG‑CATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATG‑TCCTTATTATGACGGGAAATGC‑CACCCTTTTTACCTT‑AGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAA  >  NC_000913/908943‑909415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A