Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F18 I0 R1 274 115.5 2655328 86.7% 2302169 226.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,639,706 A→C 12.5% C118G (TGT→GGT)  rrrQ ← Qin prophage, putative lysozyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,639,7060AC12.5% 284.9 / 4.9 121C118G (TGT→GGT) rrrQQin prophage, putative lysozyme
Reads supporting (aligned to +/- strand):  ref base A (53/47);  new base C (5/15);  total (58/63)
Fisher's exact test for biased strand distribution p-value = 2.76e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

TCCGACGATAAATCCTGACAACACGAGAAAAATCACCATGAATATCTGATTCACTGTTCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATG  >  NC_000913/1639474‑1639936
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                          
tCCGACGATAAATCCTGACAACACGAGAAAAATCACCATGAATATCTGATTCACTGTTCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCCCCAACATTCACCCGCTTCTAAA‑ACGACGCCGGGAAAAACTCAcc                                                                                                                                                                                                                                      >  1:828930/1‑239 (MQ=255)
tCCGACGATAAATCCTGACAACACGAGAAAAATCACCATGAATATCTGATTCACTGTTCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAAT‑CACCAGCGAATCGCTTCGCA‑TGCACCTTTCCGATCACCAGCATTACGCGCCTTATAAA‑ACCTCGCAGGAAAACAATTACCg                                                                                                                                                                                                                                     >  1:268629/1‑239 (MQ=255)
  cgacgaT‑AACCCTGACAAAACGAGAAAAATCCCCATGAATATCTGATTCACTTTTCTATCCCCCAGCAGGTTAA‑TGCGCTTTCCTGGCCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCggg                                                                                                                                                                                                                                   <  2:556145/239‑1 (MQ=255)
   gacgaTAAATCCTGACAACACGAAAAAAATCCCCATGAATATCTGATTCACTGGTCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCggg                                                                                                                                                                                                                                   <  2:707752/239‑1 (MQ=255)
    acgaTAAATCCTGACAACACGAGAAAAATCACCATGAATATCTGATTCACTGTTCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGAATTCAGCCGCTTATAAA‑CCTTCGACGGGAAACCCTTACCggg                                                                                                                                                                                                                                   >  2:524044/1‑238 (MQ=255)
     cgattaagtcTGACAACACGAGAAAAATCACCATGAATATCTGATTCACTGTTCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGc                                                                                                                                                                                                                                 <  2:655730/230‑1 (MQ=255)
       ttAACTCCTGAAACCACGAGAAATACCCCCAGGAAAACCTGAATCGCTGTACTATCCCCCAGCAGGGTTA‑GTAGCTCTCCCGGGAACGACGTAAACCCTGATCTTTGCAGTTATTTGAACCAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAAACGCTTCGCA‑TGCATCTTTACGATCACCCGCATTCAGCCGCTTATAAA‑CCGTCGACGGGAAACCCTTACCGGGGCCa                                                                                                                                                                                                                               <  1:1082466/238‑1 (MQ=255)
        tAAATCCTGAAAACACGAGAAAAATCACCATGAATATCTGATTCACTGTTCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCa                                                                                                                                                                                                                               <  2:828930/238‑1 (MQ=255)
                gACAACACGAGAAAAATCACCATGAATATCTGATTCACTGTTCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCGTTAATCCACCAGCGAATCGCTTCGAC‑TGCACATTTACGTTCACCAGCATTCAACCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGAGGCCAAGGTCATA‑g                                                                                                                                                                                                                     >  1:1126431/1‑239 (MQ=255)
                  caccccGAAAAAAATCCCCATGAATATCTTATTCACTGTTCTATCCCCCAGCAGGTTAA‑TGCCCTCGCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑ggg                                                                                                                                                                                                                   <  1:767644/234‑1 (MQ=255)
                     cacGAGAAAAATCACCATGAATATCTGATTCACTGTTCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCCCTTATAAA‑ACGTCGACGGGAACCCCTTACCGGGGCCAATGTTATA‑GGGACa                                                                                                                                                                                                                >  2:1087795/1‑239 (MQ=255)
                                   ccATGAATTTCTGATTCACTGTTCCTACCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑c                                                                                                                                                                                                <  2:1007939/239‑1 (MQ=255)
                                    cATGAATATCTGATTCACTGTTCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGCAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCACCATTCAGCCGCTTATAAA‑ACGTCGCCGGGAAACACTTACCGGGGCCCACGTTATA‑GGGAAAAAAAGAC‑GCAAAA‑cc                                                                                                                                                                                               >  1:1055121/1‑239 (MQ=255)
                                      tGAATATCTGATTCACTGTTCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑ccc                                                                                                                                                                                              >  2:723704/1‑238 (MQ=255)
                                        aggccagggttccttgtttttccccccAGCAGGTTAA‑TGCGCTCCCCGGGTCCCGGCGAATAACCGGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCt                                                                                                                                                                                           <  1:599733/217‑1 (MQ=255)
                                              tGATTCACTGTTCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATCAA‑ACTTCGCCGGGAAACACTTACCGGGGCAAAGGTTATA‑GGGACAAAATAAC‑GCGAAC‑CCCCATTTTtgt                                                                                                                                                                                     >  1:912583/1‑239 (MQ=255)
                                                aTTCACTGTTCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCCTTCAGCCGCTTATAAA‑ACGTCGACAGGAAACACTTACCGGGGCCAATGTTATA‑GGGAAAAAAtacggcaaaacaccctttttgggg                                                                                                                                                                                     >  1:672988/1‑216 (MQ=255)
                                                aTTCACTGTTCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTAACGACGAATAACCTGCCCGTAACAGTTATTTGAACGAGTGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATAGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGCAACCCTTACCGGGGCCAATGTTATA‑GGGCAAAAAGGAC‑GCGAAA‑CCCGCTTTTTgggg                                                                                                                                                                                   >  1:1170859/1‑239 (MQ=255)
                                                  tcccTGTTCCTTCCCCCAGCAGGTTAA‑TGGCCTCCCCCGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAAGGCGGTAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGtt                                                                                                                                                                                 <  1:1300442/236‑1 (MQ=255)
                                                  gctcttttCTACCCCCCGACAGGTTGA‑TTGCCCCCCCTGGTACCGCCGAATAGCCCGACCCGAGAAGTTAGTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAAGCGCTTCGCA‑TGCACCTGTACGATCACCAGCATTCAGCCGCTTATAAA‑CCGGCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGtt                                                                                                                                                                                 <  1:706837/233‑1 (MQ=255)
                                                    cgggttttctCCCCCCGCAGGTTTA‑TGCCCTCCCCTGGTCACTACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCCCCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGtt                                                                                                                                                                                 <  1:749327/228‑1 (MQ=255)
                                                      ttttttttCCCCCAAAAGTTTAA‑TGGGCTCCCCTGGTCACGACGAATACCCTGAACGTAACAGTTTTTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCCCCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGt                                                                                                                                                                             <  1:829942/232‑1 (MQ=255)
                                                      ttttCTATCCCCTAGCATGTTAA‑TGCCGTCCCCGGGTCTCGACTGATACACCGACCGTAACATGGTTTTAACCGAATGCGGCAATCACTGCCGCCATCCTTAATCCACCATGTAATCGCTTCGCA‑TGCAACTTTACCATCACCAGCAATCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGTGCAAATTTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGt                                                                                                                                                                             <  1:473428/237‑1 (MQ=255)
                                                      tGTTCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGt                                                                                                                                                                             <  2:777989/239‑1 (MQ=255)
                                                      tGTTCTATCCCCCAACATGTTAA‑TGCGCCCCCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGAAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGt                                                                                                                                                                             <  1:723704/239‑1 (MQ=255)
                                                        ttCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGCTCACCAGCATTACGCCGCTTATCAA‑ACGCCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCCATTTTTGGGGGTCGGCCa                                                                                                                                                                           >  1:801189/1‑239 (MQ=255)
                                                        ttCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACCCTTACCGGGGCCCATTTTATA‑GGGCCAAAATGAC‑CCGAAA‑CCCCCTTTTTGTGGTTCGGCCa                                                                                                                                                                           >  1:1305490/1‑239 (MQ=255)
                                                        ttCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGGGTTTCGGCCa                                                                                                                                                                           >  2:528507/1‑239 (MQ=255)
                                                          cTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGt                                                                                                                                                                         >  2:1165293/1‑239 (MQ=255)
                                                           tATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑TCCGCTTTTTGTGGTTCGGTCAggg                                                                                                                                                                        >  2:277102/1‑237 (MQ=255)
                                                            accccccAGCAGGTTAA‑TGCGGTCTCCTGGTCCCGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTgg                                                                                                                                                                       <  2:910883/237‑1 (MQ=255)
                                                            accccccAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTgg                                                                                                                                                                       <  2:710551/237‑1 (MQ=255)
                                                            aacccccAGCAGGTTAA‑TGCGCTCTCCTGGGCCCGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTgg                                                                                                                                                                       <  2:545875/237‑1 (MQ=255)
                                                            aTCCCCCAGCATGTTAAGTGC‑CTCTTCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGCCCGCCATCCGTAAGCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTgg                                                                                                                                                                       <  2:654174/239‑1 (MQ=255)
                                                            aTCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTgg                                                                                                                                                                       <  2:1325987/239‑1 (MQ=255)
                                                            aTCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTgg                                                                                                                                                                       <  2:672988/239‑1 (MQ=255)
                                                            aTCCCCCAGCAGGTTAA‑TGCCCTCTTCTGGGCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTgg                                                                                                                                                                       <  2:1190110/239‑1 (MQ=255)
                                                             tCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATAGGGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTgg                                                                                                                                                                       <  2:27699/239‑1 (MQ=255)
                                                                 ccAGCGGGTTAA‑GCGGCTCCCCGGGGCCCGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAACCCCCCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACtt                                                                                                                                                                  <  1:674641/239‑1 (MQ=255)
                                                                  cagcagGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGTCCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCACCGAATCGCTTCGCC‑TGCACCTTTACGATCACAAGCATTCGGCCGCTTCTCAA‑ACGTCGCCGCGAACCCCTTACCGTGGCAAAGGTCACA‑GGGCAAAAGTGCC‑GCGGTA‑CCCGCttttttgtgttgtttaagtggtcatgt                                                                                                                                                                 >  1:324628/1‑217 (MQ=255)
                                                                  cagcagGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGTAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGAACttt                                                                                                                                                                 >  2:1265956/1‑239 (MQ=255)
                                                                  cagcagGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTTTGGTTCGGTAAGTgg                                                                                                                                                                       >  2:749327/1‑233 (MQ=255)
                                                                  cagcagGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACttt                                                                                                                                                                 >  2:674641/1‑239 (MQ=255)
                                                                  cagcagGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAA‑GCGATA‑CCCGCATTTTGTGGTTTGGTCCGGGGAACttt                                                                                                                                                                 >  1:183991/1‑239 (MQ=255)
                                                                          tAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGCTCACCAGCATTCAGCCGCTTCTAAC‑ACGTCGTCGGGACACACTTACCGGGGCCACTGTTATC‑GGTACAAAATGAC‑GCGATA‑ACCGCTTTTTGTGTTTCGGTAAGGGGGACTTTTAttttc                                                                                                                                                          >  1:334489/1‑237 (MQ=255)
                                                                            a‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGAGGGAATTTAata                                                                                                                                                             >  2:1133750/1‑233 (MQ=255)
                                                                              cgcgcTCTCCTGTGCAAGGCGAATAACCTGACCGGAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCt                                                                                                                                                      <  2:1015678/238‑1 (MQ=255)
                                                                                   tctcCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGCCGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAA‑GACACCGAAA‑CCCGCTTTTGGGGGTCCGGTCA‑TGGGACATTaaaaattggcccccacc                                                                                                                                                >  1:1303246/1‑224 (MQ=255)
                                                                                   tctcCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCAc                                                                                                                                                  >  2:908241/1‑238 (MQ=255)
                                                                                   tctcCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGGGGTTCGGTCAGTGGTACATTAATAATGCGcaccac                                                                                                                                                  >  1:210972/1‑238 (MQ=255)
                                                                                     tcCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCa                                                                                                                                               >  2:1107432/1‑239 (MQ=255)
                                                                                          gTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCAACGGGAAAAACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGCGGGTCGGTACGGGGGACTTAAAAATTGCGCTCCACCCaatcaa                                                                                                                                          >  1:208318/1‑234 (MQ=255)
                                                                                               gacgaATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTTTGGTTCGGTCAGTGGAACTTTAATATTGCGCTCCACCCATGCCAgcg                                                                                                                                       >  2:560057/1‑237 (MQ=255)
                                                                                               gacgaATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGccgacg                                                                                                                                       >  2:752909/1‑233 (MQ=255)
                                                                                               gacgaATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAACGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAAGGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCCCTTTTTTGGGTTCGTGAAGGGGGACTTTAATAATGCCCCCCACCCATCCCAgcg                                                                                                                                       >  1:497770/1‑237 (MQ=255)
                                                                                                  aaatacccgtacgctAACAATTATTTGAACAAATGCCGCAATCGTCCTCTCCATCCTTAACCATCCATCGAATCGTTTCGCC‑ATCCCCCTTACGAACACCAGCATTCAGCCTCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCAGGCCAGCGCCTTa                                                                                                                                  <  1:1087795/225‑1 (MQ=255)
                                                                                                       aCCTGATCTTAAACGTTGTTTTAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTTGCA‑TGCTCCTTTACGGTCAGCAGCATTCAGCCGCGTATAAA‑ACGTCGACGGGAAACACGTGCCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTGTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACg                                                                                                                             <  1:6064/239‑1 (MQ=255)
                                                                                                       aCCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACg                                                                                                                             <  2:585782/239‑1 (MQ=255)
                                                                                                            aCCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCaa                                                                                                                        <  2:151375/239‑1 (MQ=255)
                                                                                                                  aCAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCATCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACAAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑CGGCGCAAATGAC‑GCCACACCCCGCTTTTTG‑GGTTCGGCCAGTGGACCGTTAATATTCCTCTCAACCCACGCCAACCCCCTATCCCCCCCAAAGGCGt                                                                                                                  >  1:1094011/1‑239 (MQ=255)
                                                                                                                      ttATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACg                                                                                                                             >  2:238309/1‑224 (MQ=255)
                                                                                                                          gtGAACCAATCCGGCACTCGCGCTCCACATCCTTAACCCCCCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCCCCAGCATGCAGGCGCTTAAAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCACTGTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTgg                                                                                                          <  1:528507/238‑1 (MQ=255)
                                                                                                                              aCGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTc                                                                                                        >  2:667160/1‑237 (MQ=255)
                                                                                                                                  aTGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGa                                                                                                                                                                                                                  <  1:45893/129‑1 (MQ=255)
                                                                                                                                  aTGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGa                                                                                                                                                                                                                  >  2:45893/1‑129 (MQ=255)
                                                                                                                                           tcccgTCCGCCATCCTTAAGCCACCAGCCAATCGCTTCGCA‑TGCACCTTTACGATCCCCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGa                                                                                        <  2:938619/236‑1 (MQ=255)
                                                                                                                                           taccttCCGCAATCCTTCATCCACCCGTGCATCGCGT‑GCAGTTCCCCTTTACCAACCCCCGGTCTCAGCCGCTTAGAAA‑ACATGCACGGGAAACACGTACCGGGGCCAAGGTTATA‑GGGATAAAATGAC‑GCGATC‑CCCGCTTTTTGTGGTTCGGTCAGTTGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATCGCGTTGACCTGGTCGCA‑TTTTTCCTTCGa                                                                                        <  1:277102/234‑1 (MQ=255)
                                                                                                                                                tCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGTCAAAATGAC‑GCGATA‑CCCGCTTTTTGGGGTTCGGTCAGTGGTACTTTAATATTGCGCCCCACCCCTGCCAGCGCCTTATCACGCCCAATGGCGTTGACCTGGT‑GCACTTTTTccctggctcttt                                                                                   >  1:751267/1‑229 (MQ=255)
                                                                                                                                                tCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTTACCTGGTCGCA‑TTTTTCCTTCGACAGtt                                                                                   >  2:1217334/1‑239 (MQ=255)
                                                                                                                                                       tatctAACCCACCTGCGAACCGCTTAGCA‑TGCCCCTTAATATTCAGTTGCAGTCAGCCGC‑TATAAACACATAGACGGACAACACTTACCGGGGCCAATGTTATA‑AGAACAAAATGAG‑CCTGTA‑CCTGCTTTTTGTGGTTCGGTCAGTGGTTCTTTAATATTGCGCGCCACCCATGCCAGCGCCTTATCGAGCGCAATGGCATTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATAtt                                                                            <  1:1156819/235‑1 (MQ=255)
                                                                                                                                                       tCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCCCCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATAtt                                                                            <  2:1301550/239‑1 (MQ=255)
                                                                                                                                                             aTCCACCAGCGAATCGCTTCGCA‑TGCCCCTTTACGATCACCAGCATTCAGGCGATTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGaaa                                                                      <  2:1126431/239‑1 (MQ=255)
                                                                                                                                                                       gAATCCCTGCGCA‑TGCACCTTGACCATCACCAGGATTCAGGCGCTGAAAAA‑ACGTCGACGGGAAACACTTACCTGGGCCAATGTTATA‑GGGACAAAATTAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATAGTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGttttt                                                            <  2:334489/239‑1 (MQ=255)
                                                                                                                                                                         tttgtataagagacagccTTTTCGATCCCCCGAATTCAGGCTGTTAAAAA‑ACGTCGACGGGAAACCCTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATC‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGttttt                                                            <  1:552121/222‑1 (MQ=255)
                                                                                                                                                                             cTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTTACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTtccttc                                                       >  2:1007963/1‑238 (MQ=255)
                                                                                                                                                                               tCGGA‑TGCACCTTTACGATCCCCAGCATTCAGCCGCTTAAAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATccac                                                    <  2:124675/239‑1 (MQ=255)
                                                                                                                                                                                      tccccgttACGATCCCCAGCATTCAGCCGTTTAAAAA‑ACGGCGACGGGAAACCCTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCTCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACtgt                                              <  2:1265117/233‑1 (MQ=255)
                                                                                                                                                                                        cccTTTTCGATCACCAGCAATCAGCCGCTTATAAA‑ACGTCGACGGGAAACCCTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTgg                                            <  2:208318/238‑1 (MQ=255)
                                                                                                                                                                                            ttACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATccacccccgttcccc                                         >  1:333027/1‑228 (MQ=255)
                                                                                                                                                                                                  tCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCACTGGCGTTGACCTGGTCGCA‑TTTTTCCTTCCACAGTTTCAACTTTGGAAAAACGGTTTTTCCATCCCCCACTGTGGCACCCCGAcc                                  >  1:1289700/1‑238 (MQ=255)
                                                                                                                                                                                                    aCCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATccacca                                                  >  2:552121/1‑221 (MQ=255)
                                                                                                                                                                                                    aCCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGa                                >  2:794232/1‑239 (MQ=255)
                                                                                                                                                                                                     ccAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGAt                               <  2:355379/239‑1 (MQ=255)
                                                                                                                                                                                                      cAGCATTCAGCCGCTTAAAAA‑ACGGCGACGGGAAACCCTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATC‑CCCGCTTTTCGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATg                              <  2:1305490/239‑1 (MQ=255)
                                                                                                                                                                                                           ttccgcaccTTATAAA‑AGTGCGACGGGAAACCCTTCCCGGGGCCAATTTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCa                         <  1:1165293/231‑1 (MQ=255)
                                                                                                                                                                                                           ttCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCa                         <  2:1055121/239‑1 (MQ=255)
                                                                                                                                                                                                           gttcggcgattAAAAA‑ACGGCGGGGGGAAACTCCTTCCGGGGCCAAGGTTAAA‑GGGAAAAAAGTGC‑GCCGTA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATGGCGCCCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTCCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCa                         <  1:667160/230‑1 (MQ=255)
                                                                                                                                                                                                              aGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCatat                      >  2:801204/1‑239 (MQ=255)
                                                                                                                                                                                                              aGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGGACTTTAATATTGCGATCCACCCATGCCAGCGCCTTACCACGCTCACTGGCGTTGACCTGGTCCCA‑TTTTTCCTTCGACAGTTGCATATTTGGAAACACGGTTTTTCCATCCACCACTGTGCCACCCCGACAGAGGGTCCatat                      >  1:1290164/1‑239 (MQ=255)
                                                                                                                                                                                                                   cTTTTAAA‑ACGGCTGCGGGAATCCTTTCCCGGGGCCAATGTTATA‑GGGACAAAAGGGA‑GCGATT‑CCCAGTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCTATGCCAGCGCCTTATCCCGCGCAATGGCTTTGACCTGGTCGCA‑TTTTTCCTTCGACATTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCTCCCCGACAGATGGCCCATATGCCAg                 <  1:1175990/239‑1 (MQ=255)
                                                                                                                                                                                                                     tataAA‑ACGTCGCCGGGAAACCCTTGCCGGGGCCAATGGTATG‑GGGACAAAATGAC‑GCGGTA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGc                    <  1:331507/234‑1 (MQ=255)
                                                                                                                                                                                                                         aa‑aCGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCAt           <  2:268629/239‑1 (MQ=255)
                                                                                                                                                                                                                         aa‑aCGGCGACGGGAAACCCTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCAt           <  2:333027/239‑1 (MQ=255)
                                                                                                                                                                                                                              gTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATcgcg       <  2:917315/239‑1 (MQ=255)
                                                                                                                                                                                                                               tCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCgg      >  2:726524/1‑239 (MQ=255)
                                                                                                                                                                                                                               tCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCCCgg      >  2:297995/1‑239 (MQ=255)
                                                                                                                                                                                                                                cGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATc          >  2:331507/1‑234 (MQ=255)
                                                                                                                                                                                                                                agACGGGAAACCCTTGCCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GGGATT‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGt     <  1:1107432/238‑1 (MQ=255)
                                                                                                                                                                                                                                 gACGGGAAACCCTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTa    <  2:497770/239‑1 (MQ=255)
                                                                                                                                                                                                                                   cGGGAAACCCTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATg  <  2:1170859/239‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                          
TCCGACGATAAATCCTGACAACACGAGAAAAATCACCATGAATATCTGATTCACTGTTCTATCCCCCAGCAGGTTAA‑TGCGCTCTCCTGGTCACGACGAATAACCTGACCGTAACAGTTATTTGAACGAATGCGGCAATCGCGTCCGCCATCCTTAATCCACCAGCGAATCGCTTCGCA‑TGCACCTTTACGATCACCAGCATTCAGCCGCTTATAAA‑ACGTCGACGGGAAACACTTACCGGGGCCAATGTTATA‑GGGACAAAATGAC‑GCGATA‑CCCGCTTTTTGTGGTTCGGTCAGTGGTACTTTAATATTGCGCTCCACCCATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCA‑TTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATG  >  NC_000913/1639474‑1639936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A