Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F27 I0 R1	83	86.1	2053078	89.1%	1829292	215.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	525,571	T→G	13.5%	W771G (TGG→GGG)	rhsD →	Rhs protein with DUF4329 family putative toxin domain, putative neighboring cell growth inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	525,571	0	T	G	13.5%	191.1 / 4.8	91	W771G (TGG→GGG)	rhsD	Rhs protein with DUF4329 family putative toxin domain, putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand): ref base T (46/27); new base G (7/10); total (54/37)
Fisher's exact test for biased strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAGGATGAGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACC  >  NC_000913/525336‑525807

tGAGGATGAGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGGGGGAAA‑CCCGGGGACGGGGGAA‑ATGGTGG‑Ggc                                                                                                                                                                                                                                             >  1:333491/1‑239 (MQ=255)

  aGGATGAGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCCAGGACGATAAAGGCCGCCTG‑ACCGGCGAAGGCCAGACGGTGGGGAA‑CCCGGAGAACGGGGAA‑CAGCAGG‑Ggc‑a                                                                                                                                                                                                                                           >  1:236531/1‑238 (MQ=39)

       gggggACTTGTCCACCTCTGGTCATACGATGAATGGGGACGTATCACTCACCGCACGGGGAACCGCGAACCGGCAGAGCAGTGGCAGTAGGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATga                                                                                                                                                                                                                                     <  1:110432/237‑1 (MQ=37)

        aGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGCAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGCTAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGGGGGGAA‑ACCGGGGACGGGGGAA‑CTGCTAG‑GGC‑GGCATgag                                                                                                                                                                                                                                    >  1:195974/1‑239 (MQ=255)

        aGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGCTAAAGGCCGCCTG‑ACAGGCGAAAGCCACACGGGGGGGAA‑CCCGGAAACCGGGGAA‑CTGCGGA‑Ggc‑agaaaggg                                                                                                                                                                                                                                    >  1:177718/1‑233 (MQ=255)

        aGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCCGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATggg                                                                                                                                                                                                                                    >  2:61288/1‑237 (MQ=255)

        aGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGGGACGGGGGAA‑CTGCtgt‑g                                                                                                                                                                                                                                               >  2:834749/1‑229 (MQ=255)

        aGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATgag                                                                                                                                                                                                                                    >  2:969864/1‑239 (MQ=255)

        aGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGGGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGG‑GGA‑CGCATgag                                                                                                                                                                                                                                    >  2:919311/1‑239 (MQ=255)

        aGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCAGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGCTAAAGGCCGCCTG‑ACCGGCGACTGCCAGACGGGGGCGAC‑CACGACGACGGGGGAA‑TTGCTGT‑ggggaactgag                                                                                                                                                                                                                                     >  2:90024/1‑230 (MQ=255)

          ggACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGG‑GGC‑GGCATGAGAc                                                                                                                                                                                                                                  >  2:582857/1‑239 (MQ=255)

              ttggccaccTCTGGTACTACGGTGAATCGGACCGTACCCCTCACCGCACGGTGAACGGCGAACCGGTAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGACACGTGAGCGAAGGCAAACGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGaaa                                                                                                                                                                                                                              <  2:67664/232‑1 (MQ=37)

              ttGTCATCCTCTGGTACTACGATTAATCGGACCGTATCACTCACCGCACGGTGGACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCCGTCTGCCAGACATCAGCCACCTGAGCGAAGGCCACCGCGTTGCCGTCCACTATGGCATGGACCATAAAGGCCGCCTG‑ACCCGCGAAGGACAGCCGGTGGAGAC‑CCCGGAAAAGGGGGGA‑AAGCGGG‑GGC‑AGCAAGGGAAGaaa                                                                                                                                                                                                                              >  1:552109/1‑239 (MQ=255)

              ttGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGaaa                                                                                                                                                                                                                              <  2:256583/239‑1 (MQ=255)

                   aTCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGc                                                                                                                                                                                                                         <  2:154962/239‑1 (MQ=255)

                           gTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGCCGGGGGGGAA‑CCCGGA‑ACCGGGGAA‑CCGCCGTGGGC‑AGCATgagcccggtcccttttaccaa                                                                                                                                                                                                                  >  2:779550/1‑220 (MQ=37)

                           gTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCCGT‑GGC‑ACCATGAGACGAAAAACGCAAACAACg                                                                                                                                                                                                                 >  2:375908/1‑239 (MQ=255)

                             tctacGGTGAACCGGACCGTTTTCCCACCCGCACGGGGAACGGCAAACCGGCAGAGCAGGGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGGGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAg                                                                                                                                                                                                               <  1:969864/238‑1 (MQ=25)

                               ttcGATGAATCGGACCGTATCCCTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCa                                                                                                                                                                                                             <  2:798200/237‑1 (MQ=255)

                                   aTGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAAAACGCATAAAACGAGAAggg                                                                                                                                                                                                          >  2:837693/1‑238 (MQ=255)

                                           gACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCaa                                                                                                                                                                                                  >  2:314605/1‑238 (MQ=255)

                                                tATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGCAAAACGCATACAACGGGCAGGGGCTGGCAAACcccg                                                                                                                                                                                            >  2:906033/1‑239 (MQ=255)

                                                tATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGCGCAGGGGCTGGCAAACcgc                                                                                                                                                                                             >  2:1019142/1‑238 (MQ=255)

                                                tATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGGGTTACCGTCCACTATGGCTATGACGGTAAAAGCCGCCTG‑ACCGCGGAATGCCAGACGGGGGAAGA‑CCCGGAGACGGGGGAA‑ATGATGG‑GGC‑AGCATCAAACCAAACACACAAACAACAGACAGGGGAGGGCaaaagcgg                                                                                                                                                                                            >  1:760453/1‑234 (MQ=255)

                                                gtgcactcacCGGACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACcgcg                                                                                                                                                                                            <  2:336852/236‑1 (MQ=255)

                                                gatccactcccggccGGTAAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACcgcg                                                                                                                                                                                            <  2:416572/225‑1 (MQ=255)

                                                        acCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGGGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCCGGTCACGcc                                                                                                                                                                                     >  2:800639/1‑238 (MQ=255)

                                                        acCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAAGACGCATACACCGAGCCGGGGCTGGCAAACCGCGTCACGCCg                                                                                                                                                                                    >  2:292427/1‑239 (MQ=255)

                                                          cgcgcgggaaaCGGAGAACCGGGAGAGCAGGGGGGGTGTGATGGCCACGGCGGGCTGACAGAAATCGGCCACCTGAGCGAAGGCCACCGTGTGGCCGGCCGCTATGGCTGTGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGa                                                                                                                                                                                  <  1:997138/230‑1 (MQ=21)

                                                          cGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGGGCCCGGGGAA‑CTGCTGT‑GGG‑AGCATGGGACGAAACACCCCAACAACCAGAAGGGGGTGGCAAACCGCGCCAcccccag                                                                                                                                                                                  >  1:815369/1‑236 (MQ=37)

                                                          cGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGa                                                                                                                                                                                  <  2:333491/239‑1 (MQ=255)

                                                          cGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACAAGCATACAACGAGCAGGGGCTGGCAAACCGCGGCACGCCgg                                                                                                                                                                                   >  2:997138/1‑238 (MQ=255)

                                                            cACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAAAACGCATACAACGGGCACGGGCGGGCAAACCCCGTCCCGCCGGAc                                                                                                                                                                                 >  2:1003192/1‑238 (MQ=255)

                                                                gTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAg                                                                                                                                                                               >  2:871011/1‑236 (MQ=255)

                                                                              ggCAGAGCAGTGGCAGTATGGTGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTAGGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGt                                                                                                                                                              <  2:760453/239‑1 (MQ=255)

                                                                                 cggccggtgggggtttggtgGCCCCGGGTGGCTGACACGCTCCGGCCCCCTGAGCGAAGGCCCCCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑CCCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGc                                                                                                                                                           <  1:582857/221‑1 (MQ=17)

                                                                                  gagCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCCCCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGc                                                                                                                                                           <  2:177718/238‑1 (MQ=255)

                                                                                    gctgggggtgttggagggCCAGGGCTTGGGGACAGACCACCGCCCCCTGACCGAAGGCCTCCGTGTTGCCGTCCACTATGGCTATGACGAGAAAGGCCGCCTG‑CCCGGCGAATGCCAGACGGGGGAGAC‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCACACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACACGCTGCCGCCGGTGGAGTGGCTg                                                                                                                                                         <  1:401309/222‑1 (MQ=17)

                                                                                         ggCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGGGCAGGGGCTGGCAAACCCCGTCACCCCGGACAGCCGGCCCCCGGTGGAGGGGCTGACGCAt                                                                                                                                                   >  2:396924/1‑239 (MQ=255)

                                                                                            cgtgtggtgGCCCTGGGTGGCGGACGAACATCCGCCGCCTGGGCGAAGGGCACCGGTGTGCCGCCCTCTGTGGCTATGACCATAAAGGCCGCCGG‑CCCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGG‑GGC‑AGCATGAGACGAAACCCGCATCCAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATgg                                                                                                                                                 <  1:890224/231‑1 (MQ=17)

                                                                                             gTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGc                                                                                                                                                <  2:171029/238‑1 (MQ=255)

                                                                                              tATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTgccgc                                                                                                                                                                       <  2:167525/214‑1 (MQ=255)

                                                                                                        aCGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTg                                                                                                                                    >  2:820351/1‑239 (MQ=255)

                                                                                                        aCGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGGGGAGTGGCTGCCGTATGGCAGCGGTTACCTg                                                                                                                                    >  2:993206/1‑239 (MQ=255)

                                                                                                          ggctggctGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCCTGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGCACCGCCGGACAGCCTGCCGCCGCGGGAGGGGCTCACGGATGGCAGGCGTGACCTCGc                                                                                                                                  >  2:749279/1‑239 (MQ=21)

                                                                                                          ggctggctGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACa                                                                                                                                                                                                                    <  1:954972/157‑1 (MQ=255)

                                                                                                          ggctggctGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACa                                                                                                                                                                                                                    >  2:954972/1‑157 (MQ=255)

                                                                                                                         aTCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGCAACACGCATACAACGCGCAGGGGCTGGCCAACCGCGTCACGCCGGACAGCCCGCCGCCGGGGGAGCGGCTGGCGTATGGCCGCGGTTACCTGGCGGG‑GAAGAAACgggg                                                                                                                  >  1:42884/1‑239 (MQ=18)

                                                                                                                         aTCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACCAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACCCCGGACAGCCTGCCGCCGGTGTACTGGCTGACGTATAGAAGCGGTCAACTGGACGG‑AATGCGGCTggg                                                                                                                  >  2:1018940/1‑239 (MQ=18)

                                                                                                                             gCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACTATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCa                                                                                                                                                                                                                        <  1:6715/134‑1 (MQ=255)

                                                                                                                             gCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCa                                                                                                                                                                                                                        >  2:6715/1‑134 (MQ=255)

                                                                                                                                   tGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGGcgcc                                                                                                         >  2:234837/1‑238 (MQ=255)

                                                                                                                                       cGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGGA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACAGCGTCCCGCCGGCCAGCCTGCCGCCGGTGGAGTg                                                                                                                                                             >  2:262052/1‑183 (MQ=255)

                                                                                                                                        gAAGGCCCCCGTGTTCGCGTCCCCTGGGGCTAGGACGATAAGGGCCGCCTG‑ACCGGCGAATGCCAGACGGGGGAAAA‑CCCGGAGACGGGGGAA‑CTGCTGG‑GGC‑AGCAGGAGACGAAACACGCATAAAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGt                                                                                                   <  1:234837/239‑1 (MQ=17)

                                                                                                                                                  gtgtTGCCGCCCTCTATGGCTATGAGGAAAAAGGCCGCCTG‑CCCGGCGAATGCCAGGCGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATAcg                                                                                         <  1:820351/239‑1 (MQ=255)

                                                                                                                                                   tgtTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGCA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTgc                                                                                                                                                                          >  1:266941/1‑158 (MQ=255)

                                                                                                                                                   tgtTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTgc                                                                                                                                                                          <  2:266941/158‑1 (MQ=255)

                                                                                                                                                      gcccccccctgtggggtaggACGATAAAGCCCGGCGG‑AGCGGCAAATGCCGGACGG‑GGAGAACCCCGGAAGCGGGGGAG‑CGGCGGT‑GGA‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCggg                                                                                     <  1:1003192/220‑1 (MQ=18)

                                                                                                                                                       gCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGACAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGGCGGGTATCCGCgggc                                                                                    >  2:288660/1‑238 (MQ=255)

                                                                                                                                                       ccccgccatcttGGCTTTGACTAAAAGGGCGCCCTG‑CCCGGGGACGCCCGGAGGGGGGAGCA‑CCGGGGGCCGGGGGAA‑CGGTGGG‑GGC‑AGCATGAGACGAAACACGCAACCACCGAGCAGGGGCTGGCAAACCGCGTCACGCTGGACGGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGa                                                                                    <  1:871011/228‑1 (MQ=255)

                                                                                                                                                        ccGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGAc                                                                                   >  2:313185/1‑239 (MQ=255)

                                                                                                                                                           tCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGGGCAGGGGCTGGCAAACCGCGTCACGCCGGCCAGCCTGCCGCCGGGGGGTGGGCTGCCGCATGGCAGGGGGTCCCTTGCGGG‑AGTGAAGCCGGGGCGGACGCCGCGGGTGGAGGAAACGCggggaagg                                                                                >  1:1002971/1‑234 (MQ=17)

                                                                                                                                                           tCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAg                                                                                 >  2:311813/1‑238 (MQ=255)

                                                                                                                                                             ccctgtGGCTATGACGATAAAGGCCTGCTG‑CCGGGTGAATTCCAGGGGGGGGAGAC‑CCCGGAGAGGGGGGAA‑CTGCTGG‑GGC‑AGCGTGAGACGAAACGCGCATACAACGGGCAGGGGCGGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGGATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCt                                                                              <  1:396924/234‑1 (MQ=255)

                                                                                                                                                             cACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑gg                                                                                                                                                                                                                                              <  1:1006182/81‑1 (MQ=38)

                                                                                                                                                             cACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑gg                                                                                                                                                                                                                                              >  2:1006182/1‑81 (MQ=38)

                                                                                                                                                              tcttggcctttGACGATAAGGGCC‑CCTGCACCGGCGAATGCCAGGGGGGGGAGAA‑CCCGGAGTCGGGGGAG‑CTGCGGT‑GGC‑AGTAAGAGACGAAACCCGCATAAATCGGGCAGGGGCGGGCAACCCGCGGCACGGCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGAAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTg                                                                             <  1:265302/229‑1 (MQ=255)

                                                                                                                                                                  tGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CCGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGGGGGGTGGCTGACGTATGGCAGCGGTTACCTCGGGGG‑GATGAAGGTGGGAGGGGCGCCGCTGGGCGGGGATAACGGGGGCAGGcgcgacc                                                                         >  1:606414/1‑233 (MQ=17)

                                                                                                                                                                     cTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGGATACCCGGGACAGGCTGCACCggg                                                                      >  2:698634/1‑237 (MQ=255)

                                                                                                                                                                        tgaacaaaaaGGCGGCGTA‑ACCGGGGGTGGCCAGGCGGCGGAGAC‑CCCGG‑GAGGGGGGAAGCTGTGGT‑GGC‑GAAAGGAGACAAACCACGCATACAAGGGGGAGGGGCGGGCAATCCGCGCCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTgaga                                                                   <  1:298903/232‑1 (MQ=255)

                                                                                                                                                                        tGACGATAAAGGCCGCCTG‑ACCGGGGAATGCCAGGCGGGGGAGAA‑CCCGGAGACGGGGGAG‑CTGCTGG‑GGC‑AGCATGGGACGAAACCCGCATCCAACGAGCAGGGGCGGGCAAACCGGGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTgaga                                                                   <  1:163458/239‑1 (MQ=255)

                                                                                                                                                                        tGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTgaga                                                                   >  2:163458/1‑239 (MQ=255)

                                                                                                                                                                        tGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGAATGGCACCGGTTACCTGGCGGG‑AATGAAGCGGGGCGGGACCCCGCGGGCCGAGTATACGCGGCAACGGCTGAAACGGgagc                                                                   >  2:669154/1‑238 (MQ=18)

                                                                                                                                                                        ggACGAGAAAGGCCGCCTG‑ACCGGCAAAGGCCGGACGGGGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACCCGCATACAACGGGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTgaga                                                                   <  2:42884/238‑1 (MQ=255)

                                                                                                                                                                          aCGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGGTACCTGGCGGG‑AATGAAGCCGGGCGGGACGCCGCTGGTCGGGGATACGCGGGACAGGCTGCACCGGgaga                                                                   >  2:265302/1‑237 (MQ=255)

                                                                                                                                                                              tAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGGgc                                                             >  2:298903/1‑239 (MQ=255)

                                                                                                                                                                              tAAAGGCCGCCTG‑ACCGACGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGCAAGGCTGCACCGTGAGAGCGTgc                                                             >  2:835397/1‑239 (MQ=255)

                                                                                                                                                                                       ccTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTTGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGGATACGCGGGACAGGCTGGACCGTGAGACGGTGCGAAGCTTCg                                                    >  2:1000760/1‑239 (MQ=255)

                                                                                                                                                                                       ccTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGGGGAGAGGCTGACGTATGGCAGCGGTTACCCGGCGGG‑TATGAAACGGGGGGGGGCGCCCCGCGTGCGGTATCCGCGGGGCCAGCTGCAcccgcaggcggcggcccgcctcg                                                    >  1:641017/1‑218 (MQ=17)

                                                                                                                                                                                       ccTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGCGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTTGCAACCCGCGTCACGCCGGCCAGCCGGCCGCCGGAGCAGTGGCTGACGTATGGCAGCGGGTACCTGGCGGGTAAGGAAG‑TGGGCGGGACGCCGCCGGTCCCGGTCAACCGGGGCAGGCGGCACCGGGGGCCGGGgcgcgagcatg                                                    >  1:605109/1‑232 (MQ=255)

                                                                                                                                                                                         gttgccGGGGAATGGCCGGGGGGGGAGAC‑CCCGGAGACGGGGGGA‑CGGCTGG‑GGC‑GGCATGAGACGAAACCCGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCgg                                                   <  1:993206/235‑1 (MQ=18)

                                                                                                                                                                                                    aTGCCAGACGGTGGAGAA‑CCCGGAGCGGGGGGAA‑CTGCGGG‑GGC‑AGCATGAAACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATggca                                         <  2:195974/238‑1 (MQ=255)

                                                                                                                                                                                                     tGCCAGACGGTGGAGAA‑CCCGGGGACGGGGGAA‑CTGCGTT‑GGC‑AGCATGAGCCGAAACACGCATACACCGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATggcagg                                       <  2:895712/239‑1 (MQ=255)

                                                                                                                                                                                                                         gggAGAGGGGGGAA‑CGGCTGG‑GGCGAGC‑GGAGACCAAACCCGCATACACCGAGCAGGGGCTGGCAAACCGCGTCCCGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGaa                    <  2:1002971/238‑1 (MQ=18)

                                                                                                                                                                                                                          ggAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGGGGGACGCCGCTGGCCGAGTATACGCGGGACAGGCGGACCCGGGGGAGGGGGGGCAGCTCCGGGGGCATGGGAGGGAGAAAAGCCggcaaacaac                   >  1:44366/1‑230 (MQ=18)

                                                                                                                                                                                                                          ggAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCa                                                                           >  2:777903/1‑183 (MQ=255)

                                                                                                                                                                                                                          ggAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCa                                                                           <  1:777903/183‑1 (MQ=255)

                                                                                                                                                                                                                          ggAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGGGGAGTGGCTGAGGTATGGCAGCGGGTACCTGGCGGG‑GATGAAGGTGGGGGGGACGCCGCTGGGCGAGTTAACGGCGGACCGGCGGGACCGTGGGACGGGGGGCAGCTTCGGGAGACTGGGCGGAGGTAATGCCGcgacggagg                   >  1:862851/1‑231 (MQ=17)

                                                                                                                                                                                                                            ggaCGGGGAAA‑CGGCTTG‑GGC‑AGCATGGGACGAACCCCGCAACCAGCGAGCAGGGGCTGGCAAACCGCGTACCGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACt                  <  2:17949/237‑1 (MQ=18)

                                                                                                                                                                                                                                 ggggAA‑CTGCTGG‑GGC‑AGCATGAGACGAAACCCGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCa             <  2:44366/238‑1 (MQ=255)

                                                                                                                                                                                                                                         tgctgT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATCAGCGGGACAGGCTGCACCGGGAGACGGGGCGCAGCTTCCGCAGCCTGGCCGGCGGGAATGCCGCCACCGAAAGGACCAGccaacaa     >  1:1016696/1‑233 (MQ=18)

                                                                                                                                                                                                                                           ctgT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCAACGGAACTGACCAggcaaaaaa    >  1:1023020/1‑230 (MQ=255)

                                                                                                                                                                                                                                             gt‑gGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGCCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCCGACGTATGGCAGCGGTTACCTGGCGGG‑ACTGAAGCTGGGGCGGACGCCCCGGGGCGAGAATACGCGGGGCTGGCGGCACCGTGACACGGCCCGCAGCCTCCGCAGCCAGGCAGGCAGTAATCCCCCAACAGAACTGAcacacacaaaaacc  >  1:997130/1‑225 (MQ=255)

TGAGGATGAGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTG‑ACCGGCGAATGCCAGACGGTGGAGAA‑CCCGGAGACGGGGGAA‑CTGCTGT‑GGC‑AGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGG‑AATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACC  >  NC_000913/525336‑525807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A