Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F9 I0 R1 260 102.5 2178490 93.2% 2030352 226.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 759,427 A→G 13.3% E241G (GAG→GGG)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913759,4270AG13.3% 284.0 / 3.3 120E241G (GAG→GGG) sucA2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base A (53/46);  new base G (4/14);  total (59/61)
Fisher's exact test for biased strand distribution p-value = 2.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 120 total aligned reads displayed.

ACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAAC  >  NC_000913/759192‑759662
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            
aCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAACTTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGGGACGCGTTAATCCCGATGCTTAACGAgat                                                                                                                                                                                                                                           >  1:1000056/1‑239 (MQ=255)
aCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAgat                                                                                                                                                                                                                                           >  1:648413/1‑239 (MQ=255)
    aCTGCGGCCCGATTGGTGCCGAGTATATGCACA‑TACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGACGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGGGACGCGGTAATCCCGATGATTAAAGAAATGAAcc                                                                                                                                                                                                                                      >  2:1071384/1‑239 (MQ=255)
         ggCCCGATTGGTTCCGAGTATAGGCACATTACCAGCACCGAAGAAAACCGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑cc                                                                                                                                                                                                                                  <  1:1076077/238‑1 (MQ=255)
         ggCCCGATTGGTCCCGAGTATCGCCACATTACCAGCACCGAAGAAAAACGCTGGATCAAACAGCGTATCGAGTCTGGTCGCCGGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑cc                                                                                                                                                                                                                                  <  1:180001/238‑1 (MQ=255)
                    tGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACa                                                                                                                                                                                                                      >  1:597367/1‑239 (MQ=255)
                           tataTGCCCATTACCAGCACCGAAGAAAACCGCGGGACCCAACAGCGTACCGAGTCGGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACg                                                                                                                                                                                                                               <  1:1081468/223‑1 (MQ=255)
                            atatGCACATTCCCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTAGCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTAACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCAc                                                                                                                                                                                                               <  1:148977/238‑1 (MQ=255)
                                   acttcccAGCCCCGAAAAAAACCGCTGGAACAAAAAGCGTATCGAGTCGGGTCGCTGCATTGTCAATAGGGAAGAAAAAAAACC‑TG‑TCTTAAGCAAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAg                                                                                                                                                                                                       <  2:1000056/234‑1 (MQ=255)
                                    aTTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGAACCCGCGAAGt                                                                                                                                                                                                      >  1:35553/1‑239 (MQ=255)
                                     ttACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑cc                                                                                                                                                                                                                                  >  1:481020/1‑210 (MQ=255)
                                     ttACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGGGACGGGTTAATCCCGATGCTTAAAGAGATGTTCCG‑CCCAGCTGGCAAAAGccggtcttttaaaaaa                                                                                                                                                                                                     >  2:1081468/1‑224 (MQ=255)
                                     ttACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGGGACGCGTTACTCCCGATGCTTAAAGAGAGGATCCG‑CCACGCGGGCAACAGCGGCACCCGCGAAGTg                                                                                                                                                                                                     >  1:31649/1‑239 (MQ=255)
                                     ttACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGCCGGGGACGCGTTAATCCCGATGCTTAAAGAGATGATCCC‑CCACGCGGGCAACAACGGCACCCGCGAAgag                                                                                                                                                                                                     >  2:91247/1‑237 (MQ=255)
                                     ttACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTCCTCG‑CTGGAAGGCGGTGACGGGTTAATCCCGAGGCTTAAAGCGATATTCCG‑CCCAGCTGGCAACAGCGGCACCCGCGacggg                                                                                                                                                                                                     >  2:205440/1‑235 (MQ=255)
                                     ttACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGCCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGGGACGCGTTAATCCCGAGGCTTAAAGAGATGATCCA‑CCCCGCTGGCCACAGCGGCACACGCGAAggg                                                                                                                                                                                                     >  2:213087/1‑237 (MQ=255)
                                           gCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CGGGAAGTCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACCCTGGCACCAGCGGCACCCGCCAAGTGGGtctc                                                                                                                                                                                               >  1:591704/1‑239 (MQ=255)
                                            cACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGATATGATCCG‑CCACCCTGGCAACACGCGCCCCCGCGAAGGGGGtccag                                                                                                                                                                                              >  2:1015213/1‑236 (MQ=255)
                                             aCCGAAAAAACACGCTGAACCCAACGGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGAGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTtct                                                                                                                                                                                                <  1:480230/236‑1 (MQ=255)
                                              ccGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCggg                                                                                                                                                                                            >  1:970485/1‑239 (MQ=255)
                                                gaagaaAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCCCGAATGGGTTCTCGGGAt                                                                                                                                                                                          >  1:716132/1‑239 (MQ=255)
                                                gaagaaAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACA‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTACAGGGATGATCCG‑CACCGCTGGCAAAAGCGGCCCCCGCGAAGTGGTTCTCggggg                                                                                                                                                                                          >  2:416285/1‑237 (MQ=255)
                                                 aagaaAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGGGATGATCCG‑CCACGCTGGCAACAGCGGCCCCCGGGACGGGGTTCCCgggggg                                                                                                                                                                                         >  2:452813/1‑236 (MQ=255)
                                                 aagaaAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATg                                                                                                                                                                                         >  1:789932/1‑239 (MQ=255)
                                                  agaaAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTAAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATgg                                                                                                                                                                                        <  1:57378/239‑1 (MQ=255)
                                                                       cggtacggggtttGGTCGGGCGACTTGAAAAAGGGAAGAAAAAAACCG‑CT‑TTCGAACCGAATT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCtgaacg                                                                                                                                                                   <  2:952801/227‑1 (MQ=255)
                                                                        cGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATCTTTAAAGGGATGATCCG‑CACCGCTGGCAAAAACGGCACCCGCGAGAGGGTTCTCGGGACGCCGCACCCGGGACGCCtgaacgg                                                                                                                                                                  >  2:243986/1‑238 (MQ=255)
                                                                         gtttCGATTCTGGGCGCGCGGTTTTCAAATGCGAAGGAAAAAAACG‑CT‑TCTAAAGCACACT‑GC‑CCGCCGCTGAAGGGCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCtgaacgtg                                                                                                                                                                 <  2:35553/236‑1 (MQ=255)
                                                                          tATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGGTGGCGCCCCGGGGGCGGCtgaacgggc                                                                                                                                                                >  2:400312/1‑239 (MQ=255)
                                                                          tATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGGTCTCGGGATGGCGCCCCGTGGCCGTCtgaacgcgc                                                                                                                                                                >  1:284387/1‑239 (MQ=255)
                                                                          tATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGACGCTTAAAGCGATGATCCG‑CCCCGCTGGCCACAGAGGACCCCGCGCGGGGGTTCTCGGGTTGGCGCAcactgggagttggcacgcgc                                                                                                                                                                >  2:725934/1‑220 (MQ=255)
                                                                          tATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTAACTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CACCGCTGGCAACAGCGGCCCACGCGAAGCGGTGCCCGGGACGGCGCACCGGGGTCGTCggaacgggc                                                                                                                                                                >  2:449667/1‑239 (MQ=255)
                                                                                 tCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGTCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACACCGCCCCCCGCCAATGGGTCTTCGGGGGGGCCCCCCCGGGTGGTCttaacgtgctggggta                                                                                                                                                         >  2:640439/1‑235 (MQ=255)
                                                                                    ggTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCCCGAAGGGGTTCTCGGGATGGCGCCCCGGGGGCGTCagaacgggctggtgaaggg                                                                                                                                                      >  2:589735/1‑236 (MQ=255)
                                                                                    ggTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCC‑CCACGCTGGCAACAGCGGCCCCCCCGAAGTGGTTCTCGGGATGGCCCACCGTGGTCGTCtgaaccggctggggaacgg                                                                                                                                                      >  2:718832/1‑238 (MQ=255)
                                                                                     gcccgccgACTTCTAATTGCGAAGAGAAAAAACC‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCtgaacgtgctggtgaacgtg                                                                                                                                                     <  2:281308/233‑1 (MQ=255)
                                                                                     gTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCttaacgtgctggtgaacgt                                                                                                                                                      >  1:536595/1‑238 (MQ=255)
                                                                                     gTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGGGGTCGTCtgaacgggggggggaacggg                                                                                                                                                     >  2:256946/1‑237 (MQ=255)
                                                                                      ccgcgccACTTTCAAGACAGAAGA‑AAAAAACGTCT‑TGTAAAGGACAGT‑GC‑CCGCCCCTGAAGGTCTTGAACTTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCCCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCtgaacgtgctggtgaacgtg                                                                                                                                                     <  2:118559/237‑1 (MQ=255)
                                                                                          gcgACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGCCGTCTGAACGTGCTGGTGAACGTGCTggg                                                                                                                                                >  1:624454/1‑239 (MQ=255)
                                                                                                 ttcaaggggagaagaAAAAAGG‑CTCTATTAAGC‑AACTCGG‑CCGGCGCTGAAGGTCTTGAACGTTCCCTCGGCGCAAATTTCCCGGGCGAAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTaaaaa                                                                                                                                          <  2:31649/227‑1 (MQ=255)
                                                                                                        cGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTCATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGCAGTGGGTCCCGGGATGGCGCACCGGGACCGTCTGAACGGGGCGGTGAACGTGCTGGGGAAAAAAcacccaa                                                                                                                                  >  2:583413/1‑233 (MQ=255)
                                                                                                             agaAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTAGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTg                                                                                                                             >  1:280759/1‑239 (MQ=255)
                                                                                                                           tcttAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGtt                                                                                                                  >  1:147532/1‑238 (MQ=255)
                                                                                                                                ggccaaCT‑GA‑CCGACGGTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAAGCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCgg                                                                                                            <  1:373574/235‑1 (MQ=255)
                                                                                                                                 gcgcccT‑GC‑CCCCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCGGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGt                                                                                                           <  2:691545/234‑1 (MQ=255)
                                                                                                                                 gCGATCT‑GC‑CCGCCGCTTAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCGGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAAGCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGt                                                                                                           <  2:922206/239‑1 (MQ=255)
                                                                                                                                   ggaCT‑GACCCGCCGCGTAAGGTCCTGAACGTTACCCGGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCCCG‑GTGGAAGGCGGTGACGGTTTAACCCCGAGGCTTAAAGGAGTGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGGGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTa                                                                                                          <  2:743319/237‑1 (MQ=255)
                                                                                                                                   gcgatctccccgccgCTGAAGGTTTTGAACTTTTCCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTGCGCG‑CTGGAAGGCGGTAGCGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGt                                                                                                           <  2:815154/230‑1 (MQ=255)
                                                                                                                                    aaCT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGGGGTCGCCTGAACGCGCTGGTGAAGGGGCGGGGTAAAAAACCCCAAAACTTGTTCGACGAGTTCGCCGGaaaa                                                                                                        >  2:564889/1‑239 (MQ=255)
                                                                                                                                     aacttgcccgccgCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGt                                                                                                           <  2:648413/230‑1 (MQ=255)
                                                                                                                                     aaacttcccggcgCTGAAGGTCTTGAACGTTACCTCGGGCCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGt                                                                                                           <  1:499253/230‑1 (MQ=255)
                                                                                                                                         gA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATa                                                                                                    <  1:452813/239‑1 (MQ=255)
                                                                                                                                          a‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATaa                                                                                                   >  1:22161/1‑239 (MQ=255)
                                                                                                                                          a‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGGCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATaa                                                                                                   >  1:688297/1‑239 (MQ=255)
                                                                                                                                               ccgcTGAAGGTCTTGAACGTTACCTCGGCGCAAACTTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGaa                                                                                               <  1:400312/239‑1 (MQ=255)
                                                                                                                                               ccgcAGAAGGTTTTAAACGTTCCCTCGGCCAAAAATCCCCTGGCGCAAAG‑CGCTTCTCG‑CGGGACGGCGGTGACCCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGaa                                                                                               <  2:866956/239‑1 (MQ=255)
                                                                                                                                                          cTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTTATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTCAACATAAAGAAACCCTCGGCAc                                                                                    >  1:292156/1‑239 (MQ=255)
                                                                                                                                                          cTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCgg                                                                                                                                                                                                                  <  1:130976/113‑1 (MQ=255)
                                                                                                                                                          cTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGCCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGGAAAAATAAAGAACACCCCGGCAc                                                                                    >  2:811251/1‑239 (MQ=255)
                                                                                                                                                              aaCGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGt                                                                                >  1:250758/1‑239 (MQ=255)
                                                                                                                                                                  ttccccggggcgaaaaTTCCC‑GGGGCAAAATGGCTTTGCG‑GTGGAAGGGGGTGCAGCGTTAATCCCGATGCTTAAAGGGATGGTCCG‑GCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACg                                                                            <  2:591704/227‑1 (MQ=255)
                                                                                                                                                                  ttcccccGGCCCAAAATTCCCTGGCCCAAAA‑CGCTTCCCG‑CCGGAAGGGGGTGACGGGTTAATCCCGATGCTTAAAGGGATGATCCG‑CCCCGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACg                                                                            <  2:940689/233‑1 (MQ=255)
                                                                                                                                                                  ttcccTCGGCCCAAAATTCCCTGCGGCAAAC‑CGCTTCCCC‑TTGGAAGGGGGTTACCCGTTAAACCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACg                                                                            <  2:899593/236‑1 (MQ=255)
                                                                                                                                                                   tACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACgg                                                                           >  1:247353/1‑238 (MQ=255)
                                                                                                                                                                   tACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGCCTGAACGTGCTGGGGAACGTGCTGGGGAAAAAACCGCAGGACTTGTTCGCCCGGTTGCCCGGTAAAAATAAAAAAAACCCCGGCCCGGGGGACgg                                                                           >  2:215606/1‑238 (MQ=255)
                                                                                                                                                                   tACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGCGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTAGCCGAGTTCGCCGCTAAACAAAAAAAAAAACCCGCAACGGGGGACGt                                                                           >  2:222175/1‑239 (MQ=255)
                                                                                                                                                                             cAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGGGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGGGACGGGAAATACcac                                                                 >  1:668820/1‑239 (MQ=255)
                                                                                                                                                                              aaaaTTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGGGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACcaca                                                                <  1:811251/239‑1 (MQ=255)
                                                                                                                                                                                aaTTCCCGGGCGCAAAG‑CGCTTTGCG‑CTGGAAGGGGGTGGGCCGTTAATCCCGATGCTGAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATg                                                              <  2:46475/239‑1 (MQ=255)
                                                                                                                                                                                     ccTGGCGCAAAA‑CGCTTCCCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCtt                                                         <  1:351662/239‑1 (MQ=255)
                                                                                                                                                                                     ccTGGCGAAAAA‑CGCTTCTCG‑CTGGAAGGGGGGGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCtt                                                         <  1:1071384/239‑1 (MQ=255)
                                                                                                                                                                                            cAAAA‑C‑CTTTTCGCCTGGAAGGCGGTGCCGCGTGAACCCCGATGCTTAAAGGAGTGACCCG‑CCCCGCTGGCAACCGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCCCCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCt                                                  <  2:597367/239‑1 (MQ=255)
                                                                                                                                                                                             aaaa‑CTCTGCTCG‑CTGGAAGGCGGTGGCGCGTTAATCCCGATGTTTAAAGGGATGATCCG‑CCACGCTGGCAACAGCGGCCCCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCt                                                  <  1:243986/238‑1 (MQ=255)
                                                                                                                                                                                             aaaa‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCAGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAGCATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCt                                                  <  1:589735/238‑1 (MQ=255)
                                                                                                                                                                                             aaaa‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAAGCCCGATGCTTAAAGGGATGACCCG‑CCACGCTTGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCt                                                  <  1:725934/238‑1 (MQ=255)
                                                                                                                                                                                             aaaa‑CGCTTCTCG‑CGGGAAGGCGGTGACGGGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGATCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCt                                                  <  1:583413/238‑1 (MQ=255)
                                                                                                                                                                                              caaacgcgttttggcgggAGGGGGGTGACGGGTTAATCCCGTGGCTTAAAGGGATGATCCG‑CCCCGCTGGCAAACGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCt                                                  <  2:247353/223‑1 (MQ=255)
                                                                                                                                                                                                   ggtgttcG‑CTGGAAGTCGGTGACGGGTGAAGCCGGAGGGTAAGATAGATGCACCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGAGGGCGCACCGGGGACGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTc                                            <  2:775197/234‑1 (MQ=255)
                                                                                                                                                                                                     ttCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAg                                                                                                                                                                                                       <  1:784124/82‑1 (MQ=255)
                                                                                                                                                                                                     ttCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAg                                                                                                                                                                                                       >  2:784124/1‑82 (MQ=255)
                                                                                                                                                                                                       ctcG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACCTAAAGAACACCTCGGCACGGGTGACGTGAAAACCCCCAGGGGCTTCTCGTCGCACTTCCAg                                         >  2:681296/1‑238 (MQ=255)
                                                                                                                                                                                                                aaGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATgg                                 <  1:91247/238‑1 (MQ=255)
                                                                                                                                                                                                                  gggggTGACGGTTTAACCCCGGTGGTTAAAGGGTTGGCCCG‑CCCCCGTGGCAACAGGGGCACCCGGGAAGTGGTTCTCGGGATGGCGCACCGTGGGCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATggcg                               <  2:508588/235‑1 (MQ=255)
                                                                                                                                                                                                                  ggCGGTGAGGCGTTAATCCCGAGGCTTAAAGAGATGATCCG‑CCACGCGGGCAACAGGGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATggcg                               <  1:232414/238‑1 (MQ=255)
                                                                                                                                                                                                                     ggggccgcgTGAATCCCGATGGTTAAAGGGATGAGCCG‑CCCCGCTGGCAACAGGGGCACCAGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGGCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGcc                            <  2:789932/233‑1 (MQ=255)
                                                                                                                                                                                                                     ggTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGGTCGCCGGTAAACATAAAGAACACCTCGCCACGGGGGACGGGAACTCCCACATGGGCTTCTCGTCTGCCTTCCAGACCGAGCGCGGccc                           >  2:824114/1‑238 (MQ=255)
                                                                                                                                                                                                                          ccctttAATCCAGATGGTTAAAGAGGCGATCCC‑CCCGCCTGGCAACAGCGGCCCCCGCGAAGTGGTTCTCGGGATGGCGCACCGGGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTg                       <  2:284387/234‑1 (MQ=255)
                                                                                                                                                                                                                             gTTAATCCCGATGCTTAAAGAGATGAGCCG‑CGCCGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCAcc                   <  1:1015213/239‑1 (MQ=255)
                                                                                                                                                                                                                                  tCCCGATGCTTAAAGAGATGACCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGGTGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGt                        <  1:167276/229‑1 (MQ=255)
                                                                                                                                                                                                                                    ccgcttcTTAAAAGGGTGATCCGCCCCCGCT‑GCAACCGGCGCCCCCGCTAAGTGTGTTGCGGGAAGGCCCCCCGGGGTCGTCTGAACGGGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggcgct            <  2:362635/233‑1 (MQ=255)
                                                                                                                                                                                                                                    ccGATGCTTAAGGGGAGGAACCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGGTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGCACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggcgct            <  1:640439/239‑1 (MQ=255)
                                                                                                                                                                                                                                    ccGAGGCTTAAAGGGATGACCCG‑CCACGCTGGCAACCGCGGCCCCCGGGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACCCCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggcgct            <  1:718832/239‑1 (MQ=255)
                                                                                                                                                                                                                                       aTGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGc                                                                                                                                     >  2:704858/1‑115 (MQ=255)
                                                                                                                                                                                                                                       aTGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGc                                                                                                                                     <  1:704858/115‑1 (MQ=255)
                                                                                                                                                                                                                                       aTGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGACCACCCCGGAACGGGCGACGTGAATTACCCCAGGGGCTTTTCGTCTGACTTCCAAACCGAAGGCGGCCTGGGGCACcgggggctggg         >  2:32156/1‑238 (MQ=255)
                                                                                                                                                                                                                                       aTGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGGGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTCGGGCACctggcgctggc         >  1:926812/1‑239 (MQ=255)
                                                                                                                                                                                                                                         gCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCCCCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATggcg                               <  1:51782/215‑1 (MQ=255)
                                                                                                                                                                                                                                            tAAAGAGATGATCCG‑CCACGCTGGCAACCGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGAAAACATAAAGAACACCTCGGCACGGGTGACGTGAATTCCCCCATGGCCTCCTCGTCTGACTTCCAGACCGATGCCGGCCTggggaccctgcagcctgcccctt    >  2:167276/1‑218 (MQ=255)
                                                                                                                                                                                                                                              aaGTGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCCCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATCCCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAAc  <  1:271233/239‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            
ACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CT‑TCTTAAGCGAACT‑GA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA‑CGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAAC  >  NC_000913/759192‑759662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A