Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F9 I0 R1 260 102.5 2178490 93.2% 2030352 226.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,146,771 T→G 10.0% V172G (GTG→GGG)  pflC → putative [formate‑C‑acetyltransferase 2]‑activating enzyme, pyruvate formate‑lyase 1‑activating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,146,7710TG10.0% 320.0 / 2.4 135V172G (GTG→GGG) pflCputative [formate‑C‑acetyltransferase 2]‑activating enzyme, pyruvate formate‑lyase 1‑activating enzyme
Reads supporting (aligned to +/- strand):  ref base T (42/74);  new base G (15/3);  total (58/77)
Fisher's exact test for biased strand distribution p-value = 2.13e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 138 total aligned reads displayed.

GATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATC‑AGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGA  >  NC_000913/4146535‑4147008
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 
            tagcAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGGCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTaa                                                                                                                                                                                                                                     <  1:369604/236‑1 (MQ=255)
                     tGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGGGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTTGGCGATGAAGGGTTGTTCGGTTTAAAAATTaaga                                                                                                                                                                                                                            >  2:968551/1‑236 (MQ=255)
                      gACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTTTGCGATGAAGTGTTGTTCGATTTAAAAATTATgg                                                                                                                                                                                                                            >  1:881041/1‑238 (MQ=255)
                      gACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGGGTGG‑CATGCGCCACTGAAACTGCCGGGGAC‑GCACCAGCCCGCAAGATATTACCCCTGGCGAAATTGGGCGATGGAGGGGGGTTGAGTTTAAAAAATATggg                                                                                                                                                                                                                           >  2:489795/1‑238 (MQ=255)
                          ctctGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGGGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGACATTGTGCGATGAAGGGTTGTTCGATTTAAAAATTATGGAcgc                                                                                                                                                                                                                        >  1:215099/1‑238 (MQ=255)
                                  cGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTAACGCTGGGGACATTGTGCGCGGAAGGGTTGTTCGGTTTAAAAATTATGGAAGCGAccaaggg                                                                                                                                                                                                               >  2:549676/1‑233 (MQ=255)
                                   gggaaggtgtgaAAGATGACATTTTCTTTCGCACGTCCGGGGGGGGGTTCACGTTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGgcg                                                                                                                                                                                                              <  2:473651/230‑1 (MQ=255)
                                     gAAGTGATGAAAGGTGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGgcgc                                                                                                                                                                                                             <  1:968551/238‑1 (MQ=255)
                                       ttttattaaaGATTACTTTTTTTTTCCCACGTCCGGCGGGGGCGTCACGTTTTTTGGCGGCGAAGTGTTAATGCAGGCGGGGTTTGCTACCTGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑ggg                                                                                                                                                                                                         <  2:963320/232‑1 (MQ=255)
                                       aGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGCCCCGCTCCAGCCGC‑ggg                                                                                                                                                                                                         >  1:1019444/1‑239 (MQ=255)
                                       aGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGGGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑ggg                                                                                                                                                                                                         >  1:811965/1‑239 (MQ=255)
                                       aGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGGGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGGGTTGTTCGATTTAAAAATTATGGAACCGACTCAGGCGC‑ggg                                                                                                                                                                                                         >  1:1021521/1‑239 (MQ=255)
                                           atgaAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTTCCCCTGGCGACATTGTGCGAGGAAGGGTTGTTCGATTTAAAAATTATGAGCGCCACCCAGGCGC‑GGGAt‑gc                                                                                                                                                                                                    >  2:862639/1‑238 (MQ=255)
                                              aaaGGTGGCTTTTTCTTTCGCCTCGCCGGCGGCGGCGTCACGCTTTCGGGCGGGAAAGTGTTAATGCAGGCGGAGTTTGCTGCCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGG‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑gtggt                                                                                                                                                                                                 <  2:812276/239‑1 (MQ=255)
                                                  aTGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGGGA‑Ag                                                                                                                                                                                            >  1:164791/1‑239 (MQ=255)
                                                   tGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGGGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGGGCGGTGAAGGGTTGTTGGATTTAAAAATTTTGGACCCGAACCCGGGGC‑GGGAG‑GTGGTGA‑AGa                                                                                                                                                                                           >  2:262536/1‑239 (MQ=255)
                                                     aCATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGGGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGGGA‑AGATg                                                                                                                                                                                         >  1:763915/1‑239 (MQ=255)
                                                      ctttttttttCGCGCGTCCGGCGGGGGGGTCCCGCTTTCTGGCGGGGAAGGGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATg                                                                                                                                                                                         <  2:43817/236‑1 (MQ=255)
                                                          ttctttcGCACGTCCGGCGGCTGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCt                                                                                                                                                                                    <  1:709150/239‑1 (MQ=255)
                                                          ttctttcGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCCC‑GGGAT‑GTGGGGA‑AGATGaaac                                                                                                                                                                                     >  1:889015/1‑236 (MQ=255)
                                                               tcGCCCGTCCGGGGGGGGGGTCACGCTTTCGGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCAc                                                                                                                                                                               <  1:308349/239‑1 (MQ=255)
                                                               gcGCACCTCCGGGGGGGGCGCCCTGCTTGTTGGCGGCGGAGTGTTTAGGCAGGGGGAGTTTGCCACCCGTTTTTTAGAGCGATGGCGGCTGGGGGGTCGGT‑CAGGAGCCATTGAAACTGCCGGAGCC‑GCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCAc                                                                                                                                                                               <  2:626424/238‑1 (MQ=255)
                                                                   ccGTCCGGCGGGGGTCTCTCCTTTTCGGGCGGGGAAGTGTTAATGCAGGGGGAGTTTGCTACCCGTTTTTTACGGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGt                                                                                                                                                                           <  2:446046/238‑1 (MQ=255)
                                                                   aCGTCCGGGGGCGGCGGCACGCTTGCGGGCGGCGAAGGGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGt                                                                                                                                                                           <  1:845193/239‑1 (MQ=255)
                                                                                  ctcccGCTTTCTGGTGGCGAAGTTTTAATGCAGGCGGCGTTTTGTGCCCGTTGTTTCCAGCGACTGCGGCTGTGGGGTGTGG‑CATGCGCCATTGAAACTGCCGGATAA‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTTTTCGATTTAAAAATTATGTACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑ATATGAACCTGCCACGCGTGCTGGAGAATCTGc                                                                                                                                                             <  1:589132/234‑1 (MQ=255)
                                                                                     ccGCTTTGTGGGGGCGAAGGTGTAAGTCAGGCGGGGTTTGCTACCCTTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGttt                                                                                                                                                         <  1:785737/238‑1 (MQ=255)
                                                                                            cTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTAT‑CATGCGCCATTGAAACTGCCGGAGCC‑GCACCAGCCAGCAACCTATTACCGCTGGAGAAAGTGTGCGAGGGAGTGTTGTTCGATTTAATAATTTTGGACGGGCACCAGGCGC‑GGGGG‑TGGGTGA‑AGATGAACCTGCCACGCGGCCTGGGGAAACGGCGtttcatggga                                                                                                                                                  >  2:421232/1‑232 (MQ=255)
                                                                                                  gggAAGTGTTAATGCGGGGGGATTTTGGTTCCCGTTTTTTACAGCGACTGGGGCTGTGGGGTGTGT‑CATGGCCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGgtgagtga                                                                                                                                             <  2:763915/236‑1 (MQ=255)
                                                                                                  gcgAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGCCGCGACTCAGGGGC‑GGGAT‑GTGGTGG‑AGATGAACCTGCCACGCGTGCTGGAGAAGCTGCGTTTGCggggggggggg                                                                                                                                            >  2:907422/1‑231 (MQ=255)
                                                                                                  gcgAAGTGTTAAGGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGgtgagtga                                                                                                                                             <  1:630608/238‑1 (MQ=255)
                                                                                                        tggtAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCAc                                                                                                                                                                               <  1:694119/195‑1 (MQ=255)
                                                                                                           tattGCGGGGGGAGTTTGCTTCCCGTTTTTTACAGCGACGGGGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAAc                                                                                                                                   <  2:639307/236‑1 (MQ=255)
                                                                                                            aaTGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGGGCTGGGGAATCTCGGTTTGCTGGGGAGTGGGGGCGCCaaag                                                                                                                                  >  1:883189/1‑237 (MQ=255)
                                                                                                                  gggggggTTTTCTACCCGTTTTGTCCAGGGCCTGCGGCGTTGGGGTGTGT‑GATGCGCCCTAGAAACTGCCGG‑GACAGTGCCAGCCAGCAAGCTATTACCGCGGGCGAAATTGTGCCATGAAGTTTTGTGCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAG‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATc                                                                                                                             <  2:572572/232‑1 (MQ=255)
                                                                                                                  ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGGCAGCAAGCTATTACCGCTGGCGAACTTGTGAGATGAAGGGTTGTTGGTATAAAAAATTAAGGACTGGACGCCGGCGG‑CGGGT‑GTGCGGACAGA‑GAACCTCCCAACGGTGCTGGAGCAACGGCGTTACCGGGGGAGGGGCGGGGTCAACGGGATcc                                                                                                                            >  2:859673/1‑239 (MQ=255)
                                                                                                                  ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTTGCGAAATTGTGGGGTGAAGGGTTGTTCGATTTAAAAATTATGGACGCAACTAACGCGC‑GGGGT‑GGGGGGG‑AGTTGAACATGCCACCCGTGCTGga                                                                                                                                                                     >  2:694119/1‑198 (MQ=255)
                                                                                                                  ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑GGATGAACCTGCCACGCGTGCTGGGGAATCTGCGTTTGCTGGGGGGGGAGGGCGTCAACGGGATc                                                                                                                             >  1:223389/1‑238 (MQ=255)
                                                                                                                  ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGt                                                                                                                                                                           <  1:668161/192‑1 (MQ=255)
                                                                                                                  ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGt                                                                                                                                                                           >  2:668161/1‑192 (MQ=255)
                                                                                                                  ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTTCTGGGGAGTGAGGGCGTCAACGGGATc                                                                                                                             >  1:379925/1‑238 (MQ=255)
                                                                                                                  ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGGCAACGGGATc                                                                                                                             >  1:700515/1‑238 (MQ=255)
                                                                                                                  ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGGGAGGGCGTCAACCTGTTc                                                                                                                             >  1:639307/1‑238 (MQ=255)
                                                                                                                  ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGGGCTGGGGAATCTGCGTTTGCTGGTGAGTGAGGGCGGCAACGTGATc                                                                                                                             >  1:458303/1‑238 (MQ=255)
                                                                                                                  ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGGGA‑AGATGAACCTTCCCACCGGGCCGGGGAATCTGCCTTTGCTGGGGGGGGAggggcgcaaccggatc                                                                                                                             >  2:90456/1‑225 (MQ=255)
                                                                                                                  ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGGGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGGGAGTGAGGGGGGCAACGTGATc                                                                                                                             >  1:270274/1‑238 (MQ=255)
                                                                                                                    gggggTTTTCTTCCCTTTTTTTACAGGGGCGGGGGGTGGGGGGTGGTT‑CATGCGCCTGTGAAACGGCCGGGGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGGGAAATTGTGGGATGAAGGGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATccc                                                                                                                           <  2:447196/234‑1 (MQ=255)
                                                                                                                    cggtgtttcttcccgtttttttGCGGGGACTGGGGCGGGGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATccc                                                                                                                           <  2:275113/222‑1 (MQ=255)
                                                                                                                         tttGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGGGAGGGCGTCAACGTGATCCCGCGttt                                                                                                                     >  1:206892/1‑239 (MQ=255)
                                                                                                                             ctgcccGTTTTTTACGGGTAATGCGGCTGTGGGGTGTGT‑CATGGGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTACGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCg                                                                                                                 <  1:901176/236‑1 (MQ=255)
                                                                                                                               tcccGTTTTTTACAGCGACGGCGGCTGTGGGGT‑TGTGCATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCt                                                                                                               <  1:777736/238‑1 (MQ=255)
                                                                                                                                    ttttttACAGCGACTGCGGCTGGGGGGTTTGT‑AATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGtgagtgag                                                                                                                                            <  2:156745/205‑1 (MQ=255)
                                                                                                                                             gCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTc                                                                                                  >  1:781716/1‑238 (MQ=255)
                                                                                                                                              cGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGGGAATCTGCGTTTGCTGGGGGGTGAGGGCGTCAACg                                                                                                                                  >  1:156745/1‑205 (MQ=255)
                                                                                                                                                aCTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACg                                                                                               >  1:360828/1‑238 (MQ=255)
                                                                                                                                                 tcgcggtttgggggTGTGT‑CTTGCGCCATTGAACCTGCCGGGGAC‑GCCACCGCCAGCAAGTTATTACCGCTGGCGAAATTGGGCGATGAAGTTTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCt                                                                                             <  2:1021521/229‑1 (MQ=255)
                                                                                                                                                 cTGGGGGTGTGGGGTGTGT‑CATGCGCCTTTGAAACTGCCGGAGAC‑GCACAAGCAAGCAAGCTTTTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTAGGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCt                                                                                             <  2:623560/239‑1 (MQ=255)
                                                                                                                                                  ttgggCTGTGGGGTTTGT‑TTGGCGCCTTTAAACCGGCCGGGGAC‑GCCCTAGCCAGCAAGGTATGACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGACAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTTACGTTc                                                                                            <  2:379925/236‑1 (MQ=255)
                                                                                                                                                  tGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGCT‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTc                                                                                            <  1:1046683/239‑1 (MQ=255)
                                                                                                                                                  tGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCt                                                                                             <  1:180459/238‑1 (MQ=255)
                                                                                                                                                     ggttgtgGGGTTGTT‑CTTGCGCCATTGAAACTGCCGGAGTC‑GCACGAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGc                                                                                         <  2:781716/236‑1 (MQ=255)
                                                                                                                                                      gCTGTGGGGTGTGG‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGc                                                                                         <  1:121965/238‑1 (MQ=255)
                                                                                                                                                               tgtgt‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAAtt                                                                                                                                                                                                                                >  1:526709/1‑97 (MQ=255)
                                                                                                                                                               tgtgt‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAAtt                                                                                                                                                                                                                                <  2:526709/97‑1 (MQ=255)
                                                                                                                                                               gtgttgcATTCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAATTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGaa                                                                                 <  1:860606/232‑1 (MQ=255)
                                                                                                                                                                 tgt‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCCCAGCGGGGGGaaaagg                                                                             >  2:518394/1‑235 (MQ=255)
                                                                                                                                                                 tgt‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTACCGCTCAGCCGGGAGAATATg                                                                             >  1:291188/1‑239 (MQ=255)
                                                                                                                                                                 tgt‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGCTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAatat                                                                              >  1:178909/1‑238 (MQ=255)
                                                                                                                                                                     cATGCCCCTTTGAACCGGCCGGGGGC‑GCGCCCGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATgcag                                                                          <  2:395400/239‑1 (MQ=255)
                                                                                                                                                                      aTGGGCCATTGAACCGGCCGGGGGC‑CCACCAGCCAGGAAGGTTTTGCCGGTGGCGAAATTTGGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATgcagc                                                                         <  2:206892/239‑1 (MQ=255)
                                                                                                                                                                       tGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGCCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGTCCAGCCGGGGGAAAATgcagca                                                                        >  1:500398/1‑239 (MQ=255)
                                                                                                                                                                       tGCGCCAGTGAAACTGGCGGAGAC‑GGACCAGCCAGCAAGTTATTACCGCTGGCGAAATGGTGCGATGAAGTGTTGTGCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATgcagc                                                                         <  1:1034867/238‑1 (MQ=255)
                                                                                                                                                                         cgcCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCAc                                                                                                <  1:980521/213‑1 (MQ=255)
                                                                                                                                                                         ccccgtggAACCTGCCGGGGGC‑GCCCCCGCCAGCAAGCTTTTACCGCTGGACAAATTGGGCGGGGAAGTGTTGTTCGATTTAAAAATTATGGACGGGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGcagcag                                                                       <  2:215099/231‑1 (MQ=255)
                                                                                                                                                                          gcCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGgc                                                                     <  1:683047/239‑1 (MQ=255)
                                                                                                                                                                           ccATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGgc                                                                     <  1:518394/238‑1 (MQ=255)
                                                                                                                                                                            cATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGgcg                                                                    <  1:90456/238‑1 (MQ=255)
                                                                                                                                                                            cATTGAAACCGGCGGGGCC‑GCCCCAGCCAGCAAGCTTTTCCCGGGGGGGAAATTGGGCGATGAAGGTTTGTTCGATTTAAAAATTATGGGCGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGgcgc                                                                   <  2:811965/239‑1 (MQ=255)
                                                                                                                                                                              ttGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGAGGTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGAGCTGGAGAATCTGCGTTTGCTGGGGAGTGAGGGAGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCCCGCTCAGCGGGGAGaaatggcgcaagggggtg                                                                 >  2:769385/1‑223 (MQ=255)
                                                                                                                                                                               tGAAACTGCCGGGACC‑GCCCCAGCCAGGAAGTTTGTACCGTTGGCGAAATTGGGGGGTGAAGTGTTGTTCGATTTAAAAATGATGGACGCGATTCAGGCGG‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTgg                                                                <  2:223389/239‑1 (MQ=255)
                                                                                                                                                                               tGAAACTGCCGGAAGC‑GCCACATCCAGCAAAGTATTACCGCTGGCGAAATTGGGCGATGAAGTGTTTGTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTgg                                                                <  1:241375/239‑1 (MQ=255)
                                                                                                                                                                                      gCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGA‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGGTCCCTGGTTTCACGCTCATCCGGGAGAATATGCAGCAGGGCCTGGGTGTCCt                                                         >  1:438656/1‑239 (MQ=255)
                                                                                                                                                                                           gaaC‑GCACCGGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATc                                                     <  1:123167/236‑1 (MQ=255)
                                                                                                                                                                                                gCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCt                                                <  1:753415/239‑1 (MQ=255)
                                                                                                                                                                                                gCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCt                                                <  1:976143/239‑1 (MQ=255)
                                                                                                                                                                                                     cgccggcAAGGTATTTCCGGGGGCAAAATGGGGGGGGAAAGTGTGTTTCGATTTAAAAATTAGGGACGCGCCTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGGCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAata                                           <  2:739128/238‑1 (MQ=255)
                                                                                                                                                                                                                tATTTCCGCGGGGGAAATTGTGCGATGAAGTTTTGTTCGATTTAAAAATTAGGGACGCGACTAAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATC‑AGGCAGAt                                <  1:639054/238‑1 (MQ=255)
                                                                                                                                                                                                                 aTTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGAAGCAGGCGCTGGATGTACTGATCCCGCTGAATATC‑AGGCAGATcc                              >  1:836463/1‑239 (MQ=255)
                                                                                                                                                                                                                    aCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGGGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGGGAATATGCAGCAGGGGCTGGAGTGACTGAGCCCCCTGAAAAAC‑AGGCAAATcaacc                           >  2:461633/1‑235 (MQ=255)
                                                                                                                                                                                                                        cTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGGGAATATGCAGCAGACGCTGGAGGTACTGATCCCGCTGAATATC‑AGGCAGATCCATCTGt                        >  1:242021/1‑238 (MQ=255)
                                                                                                                                                                                                                         tGGCGAAATTGTGCGATGAAGTGTTTTTCGATTTAAAAATTATGGACGCGACGCAGGCGC‑GGGAT‑GTGGTGA‑AGATGACCCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATC‑AGGCAGATCCATCTGtt                       <  1:752705/238‑1 (MQ=255)
                                                                                                                                                                                                                          ggCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGCCGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCggg                                                                                     <  1:532543/176‑1 (MQ=255)
                                                                                                                                                                                                                          ggCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGgc                                                                     >  2:17577/1‑192 (MQ=255)
                                                                                                                                                                                                                          ggCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGgc                                                                     <  1:17577/192‑1 (MQ=255)
                                                                                                                                                                                                                            cGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGgcgc                                                                   >  2:174285/1‑192 (MQ=255)
                                                                                                                                                                                                                              aaaTTGTGTGAGGAAGTTTTGTTAGATTTAAAATTTAGGGACGGGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGACCCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATC‑AGGCAGATCCATCTGTTACCGt                  <  2:1044184/238‑1 (MQ=255)
                                                                                                                                                                                                                                tttGTGGGTTGAAGTTTTTGTCGATTTAAAAATTTTGGACGCGTC‑CAGGGGG‑GGGGTGGGGGGGA‑AGATGAACCGGCCACGCGTGCGGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATC‑AGGCAGATCCATCTGTTACCGTTtc               <  2:883189/238‑1 (MQ=255)
                                                                                                                                                                                                                                  tgttggATTAATTGTTTTTCGATTTAAAAATTTGTGCCGCGAC‑CAGGGGCGGGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTTCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATC‑AGGCAGATCCATCTGTTACCGTTtcat             <  2:360828/234‑1 (MQ=255)
                                                                                                                                                                                                                                     gCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCGGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGATTGAGGGCGTCAACGTGATCCCGCGTTTCCCGATGATCCCTGGTTTCACGCCCAGCAGGGAGAAAATGCAGCGGGGCCGGGATGAACTGAGCCCCCCG‑ACATCAAGGCAGACCCATcgtgccggggttggtcga          >  2:550189/1‑222 (MQ=255)
                                                                                                                                                                                                                                     gCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTACCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTAACGCTCAGCCGGGGGAATATGCAGCAGGCGCTGGATGAACTGATCCCGCTGAATATA‑AGGAAGAACCATCTGTTACCGtttactaag          >  2:435308/1‑233 (MQ=255)
                                                                                                                                                                                                                                              tgttgtTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGAGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCGGGATGTACTGATCCCGCTGAATATA‑AGGCAGATCCATCTGTTACCGTTTCATCAGTACgtggc  >  1:814356/1‑234 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 
GATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGGAGAC‑GCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGC‑GGGAT‑GTGGTGA‑AGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATC‑AGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGA  >  NC_000913/4146535‑4147008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A