Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F17 I0 R1 160 98.0 2214160 89.0% 1970602 222.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 921,692 T→G 12.5% V449G (GTG→GGG)  macB → macrolide ABC transporter peremase/ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913921,6920TG12.5% 245.1 / 2.9 102V449G (GTG→GGG) macBmacrolide ABC transporter peremase/ATPase
Reads supporting (aligned to +/- strand):  ref base T (36/51);  new base G (13/2);  total (49/53)
Fisher's exact test for biased strand distribution p-value = 1.50e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

ATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACT  >  NC_000913/921455‑921914
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                               
atggggggggcggcgTTTATTTTAATGTTTTCGGCATGCCCTGAAGTAAAGGAAACACCTTAAAGCAGGGGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGtg                                                                                                                                                                                                                                <  2:686711/230‑1 (MQ=255)
aTGGGGTGGGGGGCTGTTATTTTAATGTTTATGGAAGGCCCTGCAGGGAAGGAAACCCCTTTAAGCAGGAGCA‑GCTGAACGGTCGTGCGAAGGGCGTGGTTCTCGACAGTAATACTCGCCGGCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGtg                                                                                                                                                                                                                                <  2:721675/239‑1 (MQ=255)
     gTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCCAGGCCATTGGTGTGGCGGAAGAAAAACAGTCCCAGTTTGGTAACAGTAAAGTGCTGCg                                                                                                                                                                                                                           >  1:345130/1‑239 (MQ=255)
           gtccttttttttAAATTCTATGGCTGGCCCTCCAGTGAGGGAAACTCCTTTAAACAGGAACA‑GCGGAACGGTCGTGCGCAGGTTGTGGTTCGCGACAGTAATACTCGCCGCCAGCTTTTCCCCCCTAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCt                                                                                                                                                                                                                      <  2:30690/230‑1 (MQ=255)
           ggtgttttttttAATGTTTAGGGCACGCCCTTCGGGGAAGGAAACCCCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCt                                                                                                                                                                                                                      <  2:727968/230‑1 (MQ=255)
             gtatttatttAATGGCTATGGCATGCCCTTCAGTGAAGGAAACACCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTgg                                                                                                                                                                                                                    <  1:539106/231‑1 (MQ=255)
              gttttttttAATGTCTAGGGGAGGACCTTCAGGGAAGGAACACCCTTTAATCAGGGCCA‑GCTGACCGGGCGTGCGCAGGTCGGGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGc                                                                                                                                                                                                                   <  2:909336/233‑1 (MQ=255)
                         tGTCTATGGGTTGGCCTTCAGTGAAGGAAACCCCTTTAATCAGGAGCA‑GCTGATGGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCGGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCa                                                                                                                                                                                                       <  2:203587/239‑1 (MQ=255)
                          gTCTATGGCATGACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGAGGTTTGGTAGCAGTAAAGTGCTGGGTGTCTGGCTACCTTACAgacc                                                                                                                                                                                                      >  1:461859/1‑236 (MQ=255)
                             tATGGCATGACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCCGAGGCATTGGTGTGGCGGAAGAAAAACAGGCGAGGTTTGGTAGCAGTAAAGGGCTGCGGGGTTGGCTACCTTAAAGCACG‑ag                                                                                                                                                                                                  >  1:486601/1‑238 (MQ=255)
                             tATGGCATGACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCCTTGGTGTGGCGGAAGGAAAACAGTCGATGTTTGGTAGAAGTAAAGTGCTGCGTGTCTGGCCACCTTACAACACA‑At                                                                                                                                                                                                  >  1:522318/1‑239 (MQ=255)
                             tATGGCATGACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGGGCTGGGGGTCTGGCTACCTTACAGCACG‑At                                                                                                                                                                                                  >  1:380/1‑239 (MQ=255)
                             tATGGCATGACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGAGGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCCCgtg                                                                                                                                                                                                   >  1:242513/1‑237 (MQ=255)
                               tGGCATGACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATg                                                                                                                                                                                                 >  1:404026/1‑238 (MQ=255)
                                ggctggcgctgcAGTGAAGGAAATCCCTTTAAGCAGGAGCT‑GCGGAACGGTCGGGCGCAGGGCTGGGTTCGCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTc                                                                                                                                                                                               <  2:62472/228‑1 (MQ=255)
                                     gACCTTCAGTGAAGGAAACACCTTTAATCGGGAGCA‑GTGTAACGGTCGGGCGCAGGGCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGc                                                                                                                                                                                          <  2:223325/239‑1 (MQ=255)
                                     gACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCCGGGTCATTGGTGTGGCGGAAGAAAAACAGTCGGTGGTTGGTAGCCGTAAAGTGCTGCGTGGCTGGCCACCTTACAACAGG‑AGGGCCgggg                                                                                                                                                                                          >  2:907821/1‑238 (MQ=255)
                                     gACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGACGGAAAACAGACGATTCTTGGAAACAGTCAAGTGCTGCGTGTCTGGTTACCTTACAGCACG‑ATGCCCGGGc                                                                                                                                                                                          >  1:197169/1‑239 (MQ=255)
                                     gACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGGAAAAAAGTCGGTGTTGTGTAGCAGTAAAGTTGTGGGGGGCGGGCCACCATACAGCACG‑AAATCCgggg                                                                                                                                                                                          >  2:558084/1‑238 (MQ=255)
                                     gACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTACCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCACTATGACCGCCAGCGTCATTGGTGTGGGGGAAGAAAAAAAGTACAAGTTTGGTAGCGGTAAATGGCGGCGGGGATGGCAACCTTAccgacacgtgtctgggc                                                                                                                                                                                           >  2:673556/1‑223 (MQ=255)
                                     gACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCA‑GCTGAACGGTCGGGCGCAGGTCGTGGTTCTCGACAGTAAAAATCTCCGCCAGCTTTTCCCCCATAAAGC‑AGTTGTGGTTGGCGAGGTGATTCGTGTCGGCAATATGCCCGCCCGAGTCATTTGTGTGGCGGGAGGAAAACGGTAGATGTTTAGGAGCAATATAGTGGTTGGTGTCTGGCGACCTTAAAgacagactgctggcg                                                                                                                                                                                           >  2:693594/1‑225 (MQ=255)
                                     gACATTAAGGGAAGGAAACACCTTTAATCAGGAGAA‑GCGGAACGGTCGTGCGAAGGTCGGTGTTCTCGAAAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGc                                                                                                                                                                                          <  2:242513/239‑1 (MQ=255)
                                      aCCTTCAGTGAAGGAAACACCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGGGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGGGAAACAGTCGATGTTTGGTAGCAGTAAAGGGCTGCGGGTCTGGCTACCTTACAGCCCG‑ATGTCCGGGCg                                                                                                                                                                                         >  1:40456/1‑239 (MQ=255)
                                       ccTTCAGTGAAGGAAACCCCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCg                                                                                                                                                                                         <  1:637713/238‑1 (MQ=255)
                                       ccTTCAGTGAAGGAAACACCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAATGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGa                                                                                                                                                                                        >  1:62472/1‑239 (MQ=255)
                                       ccTTCAGTGAAGGAAACACCTTTAAGCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCGCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGc                                                                                                                                                                                          <  1:749892/237‑1 (MQ=255)
                                           catgtaaGGAAACCCCTTTAAACAGG‑GCAGGGTGAACGGTCGTGCCGAGGTCGTGGTCTCCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGGCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTa                                                                                                                                                                                    <  2:740135/234‑1 (MQ=255)
                                                     aaCCCCTTTAACCAGGAGCA‑GCGGAGCGGTCGGTGGCAGGTTGTGGTTCGCGACAGTAATACTCGCCGCCAGCTTTGCCCCCATAAAGC‑AGAGGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGt                                                                                                                                                                           <  2:276949/238‑1 (MQ=255)
                                                      accccTTGAATCAGGAGCA‑GCTGAACGGTCGTGCGACGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCg                                                                                                                                                                         <  1:889346/236‑1 (MQ=255)
                                                        acCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGACCG‑ATGCCCGGGCGAGTTATGGGCCAGTCggg                                                                                                                                                                       >  1:978717/1‑237 (MQ=255)
                                                          cTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGGAGGAAAAAAGGCGGTGTTTGGTAGCAGGAAAGGGCTGCGTGGCCGGGTACCTTCCAACACG‑ATGTCCCGGCGCGTTATgggggagtgggg                                                                                                                                                                       >  2:869019/1‑228 (MQ=255)
                                                          cTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGGGCTGCGTGTCTGGCTACCTTACAGAACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTg                                                                                                                                                                       >  1:678770/1‑237 (MQ=255)
                                                            ttAATCGGGGGCC‑GCTGAACGGTCGTGCTCAGGTTGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCtt                                                                                                                                                                   <  2:461859/239‑1 (MQ=255)
                                                            ttAATCAGGAGCA‑GCTGAACGGTGTCGCGGAGGCCGTGGTTCACGAAAGTAATCCTCGCCGCGAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCtt                                                                                                                                                                   <  1:847527/239‑1 (MQ=255)
                                                            ttAATCAGGAGCA‑GCTGAACGGGCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCtt                                                                                                                                                                   <  1:801202/239‑1 (MQ=255)
                                                                    gAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGGGTCTGGCTACCTTAAAGCACG‑ATGTCCGGGGGGGTTATGGGCCGTGCGGGGCTTAACTCCAt                                                                                                                                                           >  1:568873/1‑239 (MQ=255)
                                                                     agca‑gcTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCAt                                                                                                                                                           <  1:598625/238‑1 (MQ=255)
                                                                       ca‑gcTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGGGCTGCGGGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCCGTCCGGGCTTAACTCCAtt                                                                                                                                                          >  1:43807/1‑237 (MQ=255)
                                                                           cTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGAAGCAGTAAAGGGCTGCGTGCCTGGCTACCTTa                                                                                                                                                                                                            >  1:134801/1‑185 (MQ=255)
                                                                            tGAACGGTCGTGCGAAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTc                                                                                                                                                    <  1:24896/239‑1 (MQ=255)
                                                                                      tGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCCCG‑ATTTCCGGGCGGGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGGGAaag                                                                                                                                          >  1:212188/1‑239 (MQ=255)
                                                                                      tGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGGGCTGCGGGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGGGGGTTAAATCCATTACTGGCAGGGGGAaag                                                                                                                                          >  2:327463/1‑239 (MQ=255)
                                                                                      tGCGCAGGGCGGGGTTCTCGACAGTAATACCGGCGTCCAGCTTTCCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAaag                                                                                                                                          <  1:561224/239‑1 (MQ=255)
                                                                                            ggTCGTGGTTCTCGACAGTAATTCTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGa                                                                                                                                     <  1:558084/238‑1 (MQ=255)
                                                                                             gTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATggg                                                                                                                                                                                <  1:334895/194‑1 (MQ=255)
                                                                                             gTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAATGCTGCGTGGCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTAGGGGCCAGGCGGGGCTTAACACCATTaacttactggtgaaagaaagagt                                                                                                                                   >  2:148262/1‑217 (MQ=255)
                                                                                                gTGGTTGTCGACGGTAATACTCGCCGCCAGGTTTTCCCACATAAAGG‑TGAAGGGTTTGGGGAGTAGATTCTGGTCGGGAATATGCACGCCAGAGTCATTGGTGTTGCGGAAGAAAAACAGTCGGTGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTGCCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGa                                                                                                                                <  2:188699/239‑1 (MQ=255)
                                                                                                       tcGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGGGGGTTATGGGCCAGTCGTGGCTTAAATCCATTATAGTCAGGGTGGAAAAAGGagtgtaaagggcc                                                                                                                         >  2:454647/1‑227 (MQ=255)
                                                                                                         gACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGCAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGGGCTGCGGGGCTGGCAACCTTACAGCACG‑GGGTCCGGGCGAGTTGTTGGGCAGCCGGTGCCTTACTCCCTTACATTCGGGGGGGAAAAAGGGTTTGGCaagggcga                                                                                                                       >  2:342862/1‑232 (MQ=255)
                                                                                                           tgttaactcTCGCCGCCCTCTTTTCCCCCATAAAGC‑AGATGTGGGTGGGGGGGTGATTCCGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCATTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAgg                                                                                                                     <  2:678770/231‑1 (MQ=255)
                                                                                                           cggTAATACTCGCCGGCAGTTTTTCCCCCAGAAAGC‑AGATGGGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAgg                                                                                                                     <  1:8577/237‑1 (MQ=255)
                                                                                                               aaTACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCgg                                                                                                                  <  1:907821/238‑1 (MQ=255)
                                                                                                                   ttggccctccggttttCCCCCATAAGGCGAGTTGT‑GTTGGCGGGGGGATTCTGGTCGGCAATAGTCCCGCCAGAGTCATTGGTGTGGCGGAAGAGAAACAGTCGATGGTGGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAg                                                                                                             <  2:40456/227‑1 (MQ=255)
                                                                                                                   cTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTCCTTTCAGGGGGAAAAAAGGATTTGACAGTGCCGGGGCGGAACa                                                                                                              >  1:804490/1‑238 (MQ=255)
                                                                                                                      gccgccAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCg                                                                                                                                                                         >  1:555008/1‑176 (MQ=255)
                                                                                                                      gccgccAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCg                                                                                                                                                                         <  2:555008/176‑1 (MQ=255)
                                                                                                                      gccgccAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGCAAAGTGCTGCGGGTCTGGCAACCTTACAGGCAG‑ATGTCACGGCGGGTTTTGGGGCAGTCGTGGATTAACTCCACTACTGTCATGGGGGAAGAGAGAATTGAAACCGCGGAGGCGGaaaaaaaa                                                                                                          >  2:581333/1‑233 (MQ=255)
                                                                                                                        cgccAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTTGCTTAACTCCCTTACTGTCAGGGTGAAAGAAGGATTTGCCAGCGCCGAGGCGGGACAGTAAc                                                                                                         >  1:845581/1‑238 (MQ=255)
                                                                                                                        cgccAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTCCTGTCAGGGTGGAAGAAGGATTTGACAGGCCCGAGGCGGGACAGCaaa                                                                                                         >  1:307062/1‑237 (MQ=255)
                                                                                                                               ttttCTCCCCAAAAGG‑AGATGGTGTTGGCGGGGTGTTTTGGGGGGGCAATTGGCCCGCCAGAGACATTGGGGTGGGGGAAGAAGAACAGTCGATGTTTGGTAGCCGTAAAGTGGTGGGTGTCTGGCTACCTTACAGCACG‑ATGGCCGGGCGTGTTATGGGCCGGTCGTGGGTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCAcgcg                                                                                                  <  2:558250/238‑1 (MQ=255)
                                                                                                                               ttttCCCCCATAAAGC‑GGTTGGGGTTGGCGAGGTGATTCGGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCAcgcg                                                                                                  <  1:1032302/238‑1 (MQ=255)
                                                                                                                                cttcccccctaaaagacggtgtgGTTGGCGGGGTTGTTGTGGTCGCCAAACTGCCCGCCAGGTTCATTGGTTTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGGAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCAcgcg                                                                                                  <  2:592675/219‑1 (MQ=255)
                                                                                                                                  tCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGGCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCa                                                                                                      <  1:707846/231‑1 (MQ=255)
                                                                                                                                         tAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGGAGAAAAAAAGTCTATGTTTGGTAGCAGTAAAGGGCTGCGTGTCTGGCTACCTTACAGCACG‑GTGTCCGGTCGGGTTATGGGCCAGTAGTGGCTTAACTCCATTACTGTAAGGGGGGAAGAAGTATTTGACAGGGCCGCGGTGAGAAAGCAAAACCCCCGTTTACTTTAAc                                                                                       >  2:148071/1‑239 (MQ=255)
                                                                                                                                         tAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGGTTGGTAGCAGTAAAGGGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGGCCTGGCTTAACCCCATTCACGGCAGGGGGAAAGAAGGATTTGTCAGCGCCGAGGCGGACCAGAACATCACGCGGTTatgttc                                                                                         >  2:320553/1‑232 (MQ=255)
                                                                                                                                            aGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTCCTGCCAGGGGGAAAGAAGGGTTTGACAGCGCCCAGGGGGAACAGACACTCACCGGTGTACttttgtt                                                                                      >  2:707846/1‑233 (MQ=255)
                                                                                                                                                  aTGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAAATCCAGCGTTTACTTTCACTGCGCCAc                                                                               >  1:656208/1‑239 (MQ=255)
                                                                                                                                                       gTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGaaa                                                                          >  1:494242/1‑239 (MQ=255)
                                                                                                                                                             gAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAgg                                                                     <  1:327463/238‑1 (MQ=255)
                                                                                                                                                              aGGGGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGa                                                                    <  1:723295/238‑1 (MQ=255)
                                                                                                                                                                gTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCCTTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGAttt                                                                 >  1:349516/1‑239 (MQ=255)
                                                                                                                                                                  gATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGGCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGGGAAAAAAGGGTTTTGCAGGGCCCAGACGGGAAAGCAACCCACGCGGTTACTTTCAATGCGCCACGGAAAGAAgggcttta                                                               >  2:144226/1‑233 (MQ=255)
                                                                                                                                                                   atttgggggggCATTGTGCCCGCCAGAGTCTTTGGGGGGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCtt                                                              <  2:43807/230‑1 (MQ=255)
                                                                                                                                                                     tCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGGGTTATGGGCCAGTCGTGGCTTAACTCCATTACTTTCAGGGGGAAAGAAGGGTTTGACAGCGCCGAGGCGGAACAGCAACTACCGCGCTTACTTTCACTGCGCCACGGAAAAAAGGAAtttttt                                                             >  2:797986/1‑238 (MQ=255)
                                                                                                                                                                        gtgggggcttattgccgcacgggTCATTGGTGTGGGGGAAAAAAACAATTCGAGGTTTGGGAGCGGTAAATTGCTGCGTTTCTGGTGTCCTTACAGCACG‑ATGGCCGGGGGAGTTATGGGCCAGGCGGGGCTTAACCCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCt                                                         <  2:522318/217‑1 (MQ=255)
                                                                                                                                                                         gTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCAAGGCGGAACAGCAACTCAAGCGTTTACTTTCACTGCGCCACGGAAATAAGGCTTTCTTTAccat                                                        >  1:690963/1‑237 (MQ=255)
                                                                                                                                                                              cAATATGCCCGCCAGGGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACg                                                                              <  1:781462/212‑1 (MQ=255)
                                                                                                                                                                                   tGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATggcc                                              >  1:914964/1‑237 (MQ=255)
                                                                                                                                                                                      ccGCCAGAGTCTTTGGTGTGGCGGAAGAAAAACAGTCGATGTTGGGTAGCAGTAAAGTGCTGCGTGTCTGGCTAACTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGc                                           <  1:526137/239‑1 (MQ=255)
                                                                                                                                                                                         ccAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACCCCATTACTGTc                                                                                                                                                    <  2:197337/131‑1 (MQ=255)
                                                                                                                                                                                         ccAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACCCCATTACTGTc                                                                                                                                                    >  1:197337/1‑131 (MQ=255)
                                                                                                                                                                                               ttaTTGGGTTGGGGGAAGAAAAACCGTTGATGTTGGGTTGCGGGAAAGGGCTGCGTGTCTGGCTGCCTTCCAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGaa                                   <  2:568873/236‑1 (MQ=255)
                                                                                                                                                                                                cgtgggggtgGCGGAAGTATAACAGTGGAATTTTGGTGCCAGTAAGGTGCTGCGTGTGTGGCTTCCTTGGAGTACG‑ATGTCCGTGCTAGTTTGTGGCCAGGGGTGGCTTAACTCCCTTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGaaaa                                 <  2:845581/232‑1 (MQ=255)
                                                                                                                                                                                                cATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGaa                                   <  1:151289/237‑1 (MQ=255)
                                                                                                                                                                                                  ttGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGCAAGAAGGATTTCTTTACCTGGAACATGGCCGGCGTCTTGAAAACt                               >  1:338216/1‑239 (MQ=255)
                                                                                                                                                                                                   tGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCCGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTg                              <  1:263696/239‑1 (MQ=255)
                                                                                                                                                                                                        ttggggAAGAAAATACGGCGATTTTTGGTAGGAGGAAAGTTCTGCGTGTCTGGTTACCTTACAGCGCGAATGT‑CGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGGTAAAGGAGGATCTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAATAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGaa                         <  2:380/235‑1 (MQ=255)
                                                                                                                                                                                                        tgGGGGAAGAAAAACGTTCGGTTTTTGGTGGCAGGAAAGTGCTGGGGTTCTGGCTACCTTACAGCACG‑ATGTCCGGGGGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGaa                         <  2:656208/239‑1 (MQ=255)
                                                                                                                                                                                                        tgGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGAGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGGGAAAGAAGGATTTGGCAGCGCCGAGGCGGCACATGAACTCACCCGTTTCATTTCACTGCGCCACGGCAAGAGGGAATTCTTTAACTGGGACCTCGTCCGGGCATTTAAAACTTTTGaa                         >  2:142601/1‑239 (MQ=255)
                                                                                                                                                                                                         ggCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTAAAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTCTCAGGGTGAAAGAAGGATTTGACAGACCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACAGAGCCCCGGGAAGAAGAATTTATTTACCTGGACCAAGGCCGAGGCCTTGAAAACGTTTGaaa                        >  2:770790/1‑239 (MQ=255)
                                                                                                                                                                                                         ggCGGAAGAAAAACAGTCGAAGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGGTTACTTTCACAGCGCCACGGCCAGAAGGGTTTCTTTACCTGGAACAGGGCCGCCGCTTTGAAAACTgatagaa                        >  1:1061444/1‑233 (MQ=255)
                                                                                                                                                                                                            ggAAGAAAAACAGGCGATGTTTGGTAGCAGTAAAGTGCTGAGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTACGGGCCAGTCGTGGCTTAACTCCATTACTGGCAGGGTTAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCACCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGa                     <  1:888966/239‑1 (MQ=255)
                                                                                                                                                                                                               agaaAAACAGTCGATGTTTGGTAGCAGTAAAGGGCTGCGTGGCTGGCTACCTTACAACACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACGGTCAGGGGGAAAGCAGGATTTGCCAGCGCCAGGGCGGACAAGCCACTCCCGGGGTTCCTTTCCAAGGGCCCCGGAAAGAAGGAGTTCTTTACCTGGAAACTGGACGGGGGCTTGAAAAAGGCTGaaaaaaaca                  >  2:745078/1‑234 (MQ=255)
                                                                                                                                                                                                                gaaAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGGCTTGGAAACTGTTGAAAAGACcac                 >  1:84091/1‑239 (MQ=255)
                                                                                                                                                                                                                   aaaCAGTCGTGGGTTGGTAGCAGTAAAGGTCTGGGTGTTTGGCTACCTTACAGCACG‑AGGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACGGTCAGGGTGAAAGAAGGATTTTACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACg              <  2:212188/239‑1 (MQ=255)
                                                                                                                                                                                                                   aaaCAGTCGATGTTTGGTAGCAGTAAAGTTCTGCTTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACg              <  1:576761/239‑1 (MQ=255)
                                                                                                                                                                                                                   aaaCAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACg              <  1:770790/239‑1 (MQ=255)
                                                                                                                                                                                                                   aaaCAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACg              <  1:669178/239‑1 (MQ=255)
                                                                                                                                                                                                                        gTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCCTTCCTGTCAGGGGGCCCGAAGGATTTGCCCGCGCCGCGGCGGAACACAAACCAACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTCACATGGACCATGGCAGGCGTCTTGAAAACTGTTGAAAAAACCAAACGAACtt         >  2:579464/1‑239 (MQ=255)
                                                                                                                                                                                                                               tttGGTAGCAGGAAAGTGCTGCGTTTCTGGCTTCCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGTGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACt  <  1:454647/239‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                               
ATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCA‑GCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGC‑AGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACG‑ATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACT  >  NC_000913/921455‑921914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A