Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F17 I0 R1 160 98.0 2214160 89.0% 1970602 222.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,120,818 A→G 27.5% L246P (CTC→CCC)  wcaJ ← colanic biosynthesis UDP‑glucose lipid carrier transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,120,8180AG27.5% 109.4 / 13.1 63L246P (CTC→CCC) wcaJcolanic biosynthesis UDP‑glucose lipid carrier transferase
Reads supporting (aligned to +/- strand):  ref base A (10/34);  new base G (15/3);  total (26/37)
Fisher's exact test for biased strand distribution p-value = 2.41e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACACTTTGATCGGCTTGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCA  >  NC_000913/2120581‑2121053
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
cgcatttggacgggCTTGCCATCCCTCCCGTAGGGGTGCGAGCGGAAAAACACCGGCCCTGGTGAACTGAGTTTCACCGCAAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGgag                                                                                                                                                                                                                                              <  1:510250/227‑1 (MQ=255)
 catcttgggacgtcTTGCCAGTCATGCCGTAGCGGTTCTGGCGGAAAATACCCGGCCCTGACGAACTGGGTTTCCCCGCCAGAGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGgaga                                                                                                                                                                                                                                             <  1:589306/226‑1 (MQ=255)
         aTCGGCTTGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGGAAGCGGCGTGTTGTAAAGCGGCACCACCGGGACGCCGTTCCTCTCTTCGA‑GGGGTGGATGGGGAATGTTgga                                                                                                                                                                                                                                     >  1:934668/1‑237 (MQ=255)
             gCTTGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAACGGCGTGTCGTAAAGCGGACCCCCCGGTACGCCGTTCATTTCTTCCA‑GGCGGGGATGGGGAATGTTGAAGGTa                                                                                                                                                                                                                                 >  1:947276/1‑239 (MQ=255)
               ttGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAACGGCGTGTCGTAAAGGGGCCCCACCGGTACGCCGTTCATCTCTTCGA‑GGCGGGAATGGGGAATGTTGAAAGGaaa                                                                                                                                                                                                                               >  1:275251/1‑239 (MQ=255)
                 gCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGTCAGCACAATGTCTTCCGGACGTTTGAGCAGGCGGTTAAACCCGGAAAGCGGGGGAGCGTAAAGCGGCACCCCCGGTACGGCGCTCACCTCCTCGAGGGCGCGAA‑GGAGAAGGCTGCAGGGaaaaa                                                                                                                                                                                                                             >  2:661488/1‑237 (MQ=255)
                        atGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGGGTCGTAAAGCGGCACCACCGGTACGCCGGTCATCTCTTCGA‑GGCGTGAATGGGGAATGTTGAAGGGAAAAACGTCggg                                                                                                                                                                                                                      >  1:247344/1‑239 (MQ=255)
                         tGCCGTAGCGGGTCGGGCGGAAAATACCCGGCCTTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCgggg                                                                                                                                                                                                                     <  1:1083219/239‑1 (MQ=255)
                         ggcggtggCGAGTCTGGCGGAAAATACCCGGCCCGGGTAAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCgggg                                                                                                                                                                                                                     <  1:863602/232‑1 (MQ=255)
                            cGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAACGGCGTGTCGTAAAGCGGCCCCACCGGTACGCCGTTCATCCCTTCCA‑GGGGGGAGTGGGGAATGTTTAAGGGAAAGACCTCGGGGGTc                                                                                                                                                                                                                  >  1:407794/1‑239 (MQ=255)
                              tAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAAACCCGGAAAGCGGCGTGTCGGAAAACGGCCCCACCGGTACGCCGTTCATCTCTTCCA‑GGCGTGAATGGGGAATTGTTAAGGGAAAGACGGCGGGGGTcag                                                                                                                                                                                                                >  1:1087918/1‑239 (MQ=255)
                              gaggcaGTCTGGGCGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATcag                                                                                                                                                                                                                <  1:399014/234‑1 (MQ=255)
                                  gtgTCGGGGGGAAAATACCCCGCCCGGGGGAACTGGTTTTACCCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCagcagc                                                                                                                                                                                                            <  2:247344/237‑1 (MQ=255)
                                    ttcaggCGGAAATAAACCGGCCCTGGTGAATGGAGTTTCCCCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAgcagca                                                                                                                                                                                                           <  1:757851/234‑1 (MQ=255)
                                     tCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGGGTCGGAAAGGTGCACCACCGGTACGCCGTTCATCTCTTCGC‑GGCGGGAATGGGGAATGTTGAAGGTAAAGACGTCGGGGGTCaacagccac                                                                                                                                                                                                         >  2:855197/1‑236 (MQ=255)
                                     tCTGGCGAAAAATACCCGGCCCTGGTGACCTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCAc                                                                                                                                                                                                          <  1:165824/238‑1 (MQ=255)
                                        ggCGAAAAATACCCGGCCCTGGTAAACTGGGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGaa                                                                                                                                                                                                      <  1:715521/239‑1 (MQ=255)
                                                             gTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGGATGGGGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGGGGGGTCCGCCCGTTGa                                                                                                                                                                                >  1:573972/1‑239 (MQ=255)
                                                                 accggttttCCCCGCCAGCGCAATCCAGCAACGCCCCGGGGAGATCAGGAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGa                                                                                                                                                                            <  2:1087918/233‑1 (MQ=255)
                                                                  cTGAGTTCCACCGCCAGCGCAATACAGCACAGCCCCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGAc                                                                                                                                                                           <  1:429265/239‑1 (MQ=255)
                                                                                   gccAATACCGACCAACACCGGGGGGGGCCGGAGCAGATAAGGCGCCGCCAGCAAAATGGCATCCTCGCGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGGGTGCCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGGCCAGTTTTTTCACTcgcg                                                                                                                                                          <  2:395733/237‑1 (MQ=255)
                                                                                                agcacCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGGA‑AAGGGGGGGGCCGCCCGTTGTTGGGCCAGGTTTTTCCCCCGCGCGCCGCCGCCCAt                                                                                                                                             >  1:941945/1‑239 (MQ=255)
                                                                                                  cacCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGGAAGCGGCGTGTCGTAAAGCGGCACCACCGGGACGCCGTTCATCCCTTCGA‑GGCGTGAATGGCGAATGTTTACGGGAAAAAGGTCGGGGGTCAGAGGGACCGGA‑CAGGGGGGTTTCGCCAGTTGGTTGGCCAGTTTTTTTACTcccggccccggccccactt                                                                                                                                           >  2:68273/1‑224 (MQ=255)
                                                                                                  cacCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGgag                                                                                                                                                                                                                                              >  1:701813/1‑141 (MQ=255)
                                                                                                  cacCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGgag                                                                                                                                                                                                                                              <  2:701813/141‑1 (MQ=255)
                                                                                                  cacCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGGGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑AAGGGGGGGTCCGCCAGTTGATGGACCAGtttttttccccgccccccgccggccattt                                                                                                                                           >  1:955612/1‑216 (MQ=255)
                                                                                                  cacCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGGGTCCGCCCGTTGATGGACCAGGTTTTTTCCTCGCGCGCGGTGGCACAttt                                                                                                                                           >  1:588820/1‑239 (MQ=255)
                                                                                                  cacCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGCGTCGTAAAGCGGCACCACCGGTAAGCCGGTCATCTCTTCGA‑GGCGTGAATGGGGAATGTTGAAGGGACAGACGTCGGGGATCAGCACAACCGAA‑AAGAGGGTGTCCGCCAGTGGGGGGACAAGTTTTTTCACCAGCGCGCCGGCGcacaatg                                                                                                                                           >  2:177755/1‑236 (MQ=255)
                                                                                                   cccGGGGAGATCAGCCGCAAAATAAGCGGCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGGAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTg                                                                                                                                          <  2:559389/238‑1 (MQ=255)
                                                                                                    ccGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTg                                                                                                                                          <  1:855197/238‑1 (MQ=255)
                                                                                                      gggggcgtCAGCAGCAGAAAAAGGCGCGCCAGGCCAAGTTCTTCCGCACGTTTGGGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCAt                                                                                                                                       <  2:1072072/232‑1 (MQ=255)
                                                                                                       gggAGATCAGCAGCAGCATAAGCGTCGCCAGCACAATATCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGGAAGCGGCGTGGCGTAAAGGGGCACCACCGGTACGGCGGTCCACTCTTCGT‑GGCGGGAAAGGGAGATGTTTACGGGAAAGACCGCCGGGGTCCACAGCCCCCAACCAGGGGGT‑TCCGCCAGTGGGCGGGCCCGTGTTTTCCCTCCCCCGcccgcggcaaatttg                                                                                                                                         >  2:60296/1‑223 (MQ=255)
                                                                                                       gggAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGGGTCGTAAAGCGGCACCACCGGTACGCCCTTCCTCTCTTCGA‑GGCGTGAATTGGGAAAGGTGCAGGGAAAGACGTCGGGGGTAAGCGGCCCCGAA‑CAGGGGGGGTCCGCCACTTTGTTGAGAAGGGTTTTTACTCGCccgccgccgaccaagtgcgcc                                                                                                                                      >  2:507305/1‑227 (MQ=255)
                                                                                                        ggtgttccccaggcagatgagggTCGCCCGCACAATGTTTTCCGCCCGTTTGCGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAACGCGGCTCCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATcg                                                                                                                                     <  2:455973/217‑1 (MQ=255)
                                                                                                        ggAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATc                                                                                                                                      <  1:292315/238‑1 (MQ=255)
                                                                                                         gagaTCCGCAGCAAAATAAGCCTCCCCAGCACAATGTCTTCCGCACGTTTGAGCAGGGGGTTAACCCCGGAAAGGGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATcg                                                                                                                                     <  2:955612/238‑1 (MQ=255)
                                                                                                         gagaTCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATcg                                                                                                                                     <  1:316474/238‑1 (MQ=255)
                                                                                                            aTCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCAc                                                                                                                                                                                                          >  1:410012/1‑167 (MQ=255)
                                                                                                            aTCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCAc                                                                                                                                                                                                          <  2:410012/167‑1 (MQ=255)
                                                                                                                 ccacaaaaTAAGCTTCCCCAGCACAATGTTTTCCGCACGTTTGGGCTGGCGGTGAACCCCGGAAAGGGGCGTGTCGTAAAGCGGCCCCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGGCGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGa                                                                                                                            <  2:931371/233‑1 (MQ=255)
                                                                                                                   gcagAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGAGGCGTGTCGTAAAGCGGGCCCACCGGTACGTCCTTCCTCTCTTTGA‑GGCGTGAATGGGGAATGTTGAAGGGCAAGACGCCGGGGATCCGCCGCGCCCAA‑CAGGGGGGGTTCGCCACCTGAGTGACCAGATTTTTTTCCCTCGCGCCCGCGCACCCTTGCATccccctaaaaaag                                                                                                                          >  2:517542/1‑227 (MQ=255)
                                                                                                                     ggaaacagcgtccccgcgacaATGGCTTCCGCAGGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTGCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGAc                                                                                                                           <  2:588820/218‑1 (MQ=255)
                                                                                                                               tCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGGGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGGTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGGTTTAGACG‑TTTTGaag                                                                                                                 >  1:931371/1‑234 (MQ=255)
                                                                                                                                 gCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCTCGCCGTCGCACATTTGCATCGCGATATAGCCG‑TTATGAATCtttc                                                                                                            >  1:198788/1‑236 (MQ=255)
                                                                                                                                              tttCCGCCCGTTTGAGCAGGGCGTTAACCCCGGAAAGGGGCGGTTCGTAAAGCGGCCCCTCCGGTACGCCGTTCATCTGTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTc                                                                                               <  2:275251/237‑1 (MQ=255)
                                                                                                                                                    cccGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCCCCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGAcc                                                                                        <  1:68273/237‑1 (MQ=255)
                                                                                                                                                               cggtggTTAACCCGGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCCCCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCa                                                                              <  1:517542/234‑1 (MQ=255)
                                                                                                                                                                 gCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGGACGCCGTTCATCTCTTCGA‑GGCGTGAATGGGGAATGTTGAAGGTAAAGACGGCGGGGATCAGCAGCACCGAA‑CAGGGGGGGTCCGCCCGTTGATGGGCCAGTTTTTTCACCCGCGCGCCGTCCCCACTTTGCATCGCGCAATAAACGCTTTTAAATTTTCCCCCCTTCcgctcccccccccccctggtgtggg                                                                            >  2:788789/1‑217 (MQ=255)
                                                                                                                                                                         ccctgagagccgcgggtctttAAAGGGGCACGCCCGTGATGCAGTTCACTCCTTCCG‑GGCGGGACTGGAGAATTGTGAAGGTAAAGGCGTCGGGGATCAGCAGCACCGAA‑CAGGTGTTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACACTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGcc                                                                   <  2:410019/219‑1 (MQ=255)
                                                                                                                                                                         ccccGGAAAGCGGGGTTTCGTAAAGCGGCACCACGGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGcc                                                                   <  2:407794/239‑1 (MQ=255)
                                                                                                                                                                         ccccGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTTAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGcc                                                                   <  1:788789/239‑1 (MQ=255)
                                                                                                                                                                              gAAAGCGGCGTGTCGTAAAGCGTCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGCATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGCA‑CAGGTGGGTTCCGCCAGTTGGTGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCCCTTTCGCGTCCTCCACCAACTGTTCCAAGTTAACCGCCCAGTc                                                              >  2:518093/1‑239 (MQ=255)
                                                                                                                                                                                             ttaaggggcccccgcGGTTCGCCGTTCATCTTTTGGG‑GGCGTGAATGGGGAATGGTGAAGGTAAAGACGTCGGGGGTCAGCAGCCCCGAA‑CAGGGGGTGTCCGCCAGGTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACgccgcc                                               <  2:573972/225‑1 (MQ=255)
                                                                                                                                                                                              aaaGCGGCACCCCCGGTACGCCGTTCATCTCTTCGA‑GCCGTGAATGGAAAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACgccgcc                                               <  1:644095/238‑1 (MQ=255)
                                                                                                                                                                                              aaaGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACgccgcc                                               <  1:887180/238‑1 (MQ=255)
                                                                                                                                                                                                           cGGTACTCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGt                                 <  1:726797/239‑1 (MQ=255)
                                                                                                                                                                                                                  ccGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCCCGTCCTCGACCACCTGTTGCAGGTTACCCCCCCAGTCGTTCGAAACCCCCCCCGCTTTCGGGCGGTGGTaac                           >  2:254285/1‑237 (MQ=255)
                                                                                                                                                                                                                       tctcttTTCGG‑GGCGTTATGGGGAAATGTTTAAGGGAAAGGCGTCGGGGATCAGCAGCCCCGAA‑CGGGTGGGGCCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCcca                     <  2:934668/237‑1 (MQ=255)
                                                                                                                                                                                                                        cccttttCGC‑GGCGGGAATGGAGAATGTTGAAGGGAAAGCCGTCGGGGTTCCGGACCCCCGAA‑CAGGGGGGTGCCGCCAGTTGATGGACCCTTTTTTTCCCTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCccac                    <  2:941945/234‑1 (MQ=255)
                                                                                                                                                                                                                         actcgTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCccacc                   <  1:254285/238‑1 (MQ=255)
                                                                                                                                                                                                                             ttCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCTTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGCCGTTCGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACtt               >  1:337331/1‑239 (MQ=255)
                                                                                                                                                                                                                               cGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGCTATAGACG‑TTATGAATCTTGCCCGCTTTCCGGTCCTCGACCCGCGGTTGCAGGTTACCCGCCCAGTCGTTAGAAACCCCGCCCCGTTTCGGGTGGAGGTAAACGCCACCCACTCCa             >  2:449307/1‑239 (MQ=255)
                                                                                                                                                                                                                               cGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGCCCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCCCCCGGCTTCGGGTGGGGGTAAAccccccccccccca             >  2:356716/1‑233 (MQ=255)
                                                                                                                                                                                                                                gA‑GGGGTGAATGGAGAATGGTGAAGGTAAAGACGGCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCaa            <  1:356716/239‑1 (MQ=255)
                                                                                                                                                                                                                                 ggggCGTGAATGGATAATGTTGAAGGTAAAGGCGGCGGGGATCAGCAGCCCCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTCTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCaa            <  1:556639/237‑1 (MQ=255)
                                                                                                                                                                                                                                           tGGGGAATGGTTAAGGTAAAGGCGGCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCTGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCa  <  1:456335/239‑1 (MQ=255)
                                                                                                                                                                                                                                           tGGAGATGGTTGAAGGTAAAGCCGTCGGGGATCAGCAGCCCCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCa  <  1:147043/239‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
CACACTTTGATCGGCTTGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAA‑CAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACG‑TTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCA  >  NC_000913/2120581‑2121053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A