Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F17 I0 R1 160 98.0 2214160 89.0% 1970602 222.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,160,853 C→T 20.1% intergenic (‑64/‑270) sthA ← / → fabR pyridine nucleotide transhydrogenase, soluble/transcriptional repressor of fabA and fabB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,160,8530CT20.1% 217.2 / 54.8 113intergenic (‑64/‑270)sthA/fabRpyridine nucleotide transhydrogenase, soluble/transcriptional repressor of fabA and fabB
Reads supporting (aligned to +/- strand):  ref base C (38/52);  new base T (10/13);  total (48/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.60e-01

Only 100 of 115 total aligned reads displayed.

GACGGAGAGCTTTCGACGGGATGGTGCCCCAGTGGGTGCAACCGCCGCCAACATTTTGATAACGCTCGATAACTGCGACGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAG  >  NC_000913/4160618‑4161085
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        
gACGGAGAGCTTTCGACGGGATGGTGCCCCAGTGGGTGCAACCGCCGCCAACATTTTGATAACGCTCGATAACTGCGACGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAACCTTAGGAATGGGGCATGGTGGGGCTTCCCTGTTCTTTTACATAAAAGCAACAGAATGG‑AACACTTTTATCGCGGGTAAGCCaa                                                                                                                                                                                                                                       >  2:865039/1‑239 (MQ=255)
       agCTTTCGACGGGATGGTGCCCCAGTGGGTGCAACCGCCGCCAACATTTTGATAACGCTCGATAACTGCGACGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAACCCAATTGATc                                                                                                                                                                                                                                 >  1:459214/1‑238 (MQ=255)
         gtttccgcgggttgggtcccgcgTGGGTTCACCCCCCGCCAACATTTTGATAACGCTCGATAACTGCGACGCGGCGACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGTAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATccc                                                                                                                                                                                                                               <  2:434556/216‑1 (MQ=255)
         cTTTGGGCGGGATGGTGCCCCAGGGGGGTCAACCGCCGCCAACATTTTGATAACGCTCGATAACTGCGACGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATccc                                                                                                                                                                                                                               <  1:931023/238‑1 (MQ=255)
                    ttgttcccccggggggtcccgccgccgCCAACATTTTGATAACGCTCGATAACTGGGACGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATc                                                                                                                                                                                                                    <  2:364119/217‑1 (MQ=255)
                    gtttggCCCCAGGGGGTCCACCCCCCGCCAAAATTTTGATAACGCTCGATAACGGCGCGGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCTGCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATc                                                                                                                                                                                                                    <  2:885843/233‑1 (MQ=255)
                    atgtgaccgcaggggtcgtaaCCGCCGCCAACATTTTGATAACGCTCGATAACTCCGCCGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGATCCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATc                                                                                                                                                                                                                    <  1:237705/219‑1 (MQ=255)
                    agggggCCCAAGTGGGTGCAACCGCCCCCAACATTTTGATAACGCTCGATAACTGCGACGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATc                                                                                                                                                                                                                    <  1:954097/233‑1 (MQ=255)
                    aTGGTTCCCCAGTGGTTACCACCACCGCCAACATTTTGATAACGCTCGATAACTGCGACGCGCGCCCCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATc                                                                                                                                                                                                                    <  1:711041/238‑1 (MQ=255)
                    aTGGTGCCCCAGTGGGTGCAACCGCCGCCAACATTTTGATAACGCTCGATAACTGCGACGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATc                                                                                                                                                                                                                    <  1:136183/238‑1 (MQ=255)
                         cccgcggggggTGCAACCCGCGCCAAAATTTTGATAACGCTCGATAACTGGGACGCGCGAACCTGGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCt                                                                                                                                                                                                               <  2:952868/230‑1 (MQ=255)
                          ccccAGTGGGTGCAACCGCCGCCAACATTTTGATAACGCTCGATAACTGCGACGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCCCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCtat                                                                                                                                                                                                             >  1:872616/1‑239 (MQ=255)
                                  ggTGCAACCGCCGCCAACATTTTGATAACGCTCGATAACTGCGACGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGAAGTGCTTACCTGTTCTTATACATAAAATCAACAGAATGGAAACA‑TTTTATCTCGGGTAAGCCAATTGATCCCCGTCATTTATCTGCCTATTACCTGa                                                                                                                                                                                                      >  1:724100/1‑238 (MQ=255)
                                   gTGCAACCGCCGCCAACATTTTGATAACGCTCGATAACTGCGACGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAg                                                                                                                                                                                                     <  1:904945/238‑1 (MQ=255)
                                                 aaCATTTTGATAACGCTCGATAACTGCGACGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGGAACAGAATGGTAACA‑TTTTATCGCGGGTAAGTCAATTTATCCCCGTCATTTATTTTGTTATAACCTGAGAGGCcttttcttttt                                                                                                                                                                                      >  2:222650/1‑237 (MQ=255)
                                                    aTTTTGATAACGCTCGATAACTGCGACGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATc                                                                                                                                                                                                                    <  1:438853/206‑1 (MQ=255)
                                                         gtcaccggcggATCACGGCGGCGGGCGCTCCTTGCTTAACCAGGCCCATTGCGGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGGGGCATGGTAGGGCTTCCCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCt                                                                                                                                                                              <  2:194383/229‑1 (MQ=255)
                                                         gATAACGCTCGATAACTGCGACGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCt                                                                                                                                                                              <  1:950176/239‑1 (MQ=255)
                                                              cGCTCGATAACTGCGACGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACtt                                                                                                                                                                         >  1:327959/1‑239 (MQ=255)
                                                                      aaCTGCGACGCGCGGACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTg                                                                                                                                                                 <  1:153621/239‑1 (MQ=255)
                                                                      aaCGGCGACGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGGCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCt                                                                                                                                                                  <  1:865039/238‑1 (MQ=255)
                                                                       catccgccccgcgcACCTTGTTTAACCCGGCCCATGGCGGCGCGTTGGGCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAAGCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGa                                                                                                                                                                <  2:956765/230‑1 (MQ=255)
                                                                             aCGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATTTGGCTATATCCGAAGCGGCCTTTGCTTTGACTGTTTTTAACTTTTGCCCTGCCGtttt                                                                                                                                                          >  2:479815/1‑239 (MQ=255)
                                                                                  cgcACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTgg                                                                                                                                                     >  1:956765/1‑239 (MQ=255)
                                                                                         tGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCCTGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGGATGGGAACA‑TTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTAAATCCTGAGCGGCCTTGGCTTTGTCTGCTTCTTACTTTTGCCCTCACATTTTATTGAAgtttt                                                                                                                                               >  2:435266/1‑238 (MQ=255)
                                                                                             tAACCCGGCCCATTGAAGCGCCTTCGCCGCCGGGGCCGGAACCTATTGC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTTAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATcga                                                                                                                                          <  2:1098889/239‑1 (MQ=255)
                                                                                              aaCCATGACCTTTGCAGCGCTTTCGCCGCGGGGGCCGGAACCTATTAC‑GTTTGAATCTTAATGTTGTGAATGTGGCATGGTAGGGCTTGCCTGTTCTTATACATAAAAGCCACAAAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTAGcgac                                                                                                                                         <  2:312594/239‑1 (MQ=255)
                                                                                                    gCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATat                                                                                                                                    >  1:1079202/1‑237 (MQ=255)
                                                                                                    gCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATAc                                                                                                                                    >  1:1038109/1‑238 (MQ=255)
                                                                                                    gCCCATTGCAGCGCCTTCGCCGCAGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCTTAGGAATGTGGCATGGTCGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTCAGCCAATTGATCCCCGTCCTTTATCTGGCTATATCATGAGCGGCCTTGGTTTTGCCTGTTTCTTCCTTTTCCCCTGACGTTTTATTGGGTTTTTTTcatcgaacc                                                                                                                                    >  2:1049351/1‑230 (MQ=255)
                                                                                                     cctaTTGCCGCGCCTTCGCCGGCGGGGACGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATAct                                                                                                                                   <  2:124484/235‑1 (MQ=255)
                                                                                                     cccATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGc                                                                                                                                                                                                                                          <  1:728044/135‑1 (MQ=255)
                                                                                                     cccATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGc                                                                                                                                                                                                                                          >  2:728044/1‑135 (MQ=255)
                                                                                                     cccATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGATGATAct                                                                                                                                   >  1:313293/1‑238 (MQ=255)
                                                                                                     cccATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATAct                                                                                                                                   >  2:162680/1‑238 (MQ=255)
                                                                                                       cATGGCAGCGCCTTCGCCGGCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGGGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACtctc                                                                                                                                <  1:222650/239‑1 (MQ=255)
                                                                                                          tGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGTTACTCTCCGt                                                                                                                             >  1:286124/1‑239 (MQ=255)
                                                                                                                 ccTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTa                                                                                                                                                         <  1:575354/204‑1 (MQ=255)
                                                                                                                           cgGGGCCGGAACCTATTAC‑TATGGCATAGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTTTTATACATAAAAGCAACAGAATGGTAACC‑TTTTATCGCGGGTAAGCCAATTGATCACCGTCATTTATCTGACTCTATCCATAGCGGCCTATGCTTTGTCTGTTTCTTACTTTTTCCTTGCCGTTTTATTGGATTTTTATCGCTTATCCTCTCCGTTTAAACGCCAGTATTcc                                                                                                            >  2:861975/1‑239 (MQ=255)
                                                                                                                            tagagaaaagctttttAC‑TTTGGCAATATAAACGTAGGAATGTGGCAGGGTTGGGCTTTCCTGTTCTTATGCATAAAAGGAACGAAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATGCTGAGCGGCGTTTGCTTTGTCTGTTTCTTACTTTTGCACTGACGTTTTATTGGATTGGTATCGACTATGCTGACCGTTTATGCGGCAGGTTGCCg                                                                                                           <  2:671034/225‑1 (MQ=255)
                                                                                                                            ggggCCGGAACCTTGTCA‑TATGGCATTGTAATCGTAGGAAAGTGGCATGGTAGGGCTTACCTGTGCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCTGCCTTTGCTTTGTCTGGTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCg                                                                                                           <  1:861975/239‑1 (MQ=255)
                                                                                                                            ggggCCGGAACCTATTAC‑CATGGCATCGTAAGCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTACACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGGTTAAGCGGCAGGTTTCCg                                                                                                           <  1:1049351/239‑1 (MQ=255)
                                                                                                                            ggggCCGAAACCTTTTACGTAT‑GCATCGTAATTGGAGGAATGTGGCATGGTAGGGTTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCg                                                                                                           <  2:313293/239‑1 (MQ=255)
                                                                                                                                  ggAACCTATTAC‑TATTGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTa                                                                                                      <  1:745628/238‑1 (MQ=255)
                                                                                                                                  ggAACCTATTAC‑TATGGCATCGGAATCGTAGGAATGGGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTa                                                                                                      <  1:226337/238‑1 (MQ=255)
                                                                                                                                               tacggCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTACGCGGCAGGGTTCCGCTGTACGTAAAAGaaaag                                                                                         >  2:918738/4‑236 (MQ=255)
                                                                                                                                               tATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAACGAAcc                                                                                          >  1:638271/1‑238 (MQ=255)
                                                                                                                                               tATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATc                                                                                                                                                                                                                    >  1:266678/1‑116 (MQ=255)
                                                                                                                                               tATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATc                                                                                                                                                                                                                    <  2:266678/116‑1 (MQ=255)
                                                                                                                                                 tGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTTTCGACGATACTCTCCGTTTAAGCGGCAGGTTTATGATGTACGTAAAAAAACCGGc                                                                                       >  2:22894/1‑239 (MQ=255)
                                                                                                                                                 tGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTa                                                                                                      <  1:529714/224‑1 (MQ=255)
                                                                                                                                                 tGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTa                                                                                                      >  2:529714/1‑224 (MQ=255)
                                                                                                                                                 tGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGc                                                                                       >  1:377556/1‑239 (MQ=255)
                                                                                                                                                 tGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGAAACA‑TTTTATCGCGGGAAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAACCGGCAGGGTTCCGCTGTACGTat                                                                                                 >  1:671034/1‑228 (MQ=255)
                                                                                                                                                  ggCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTTTCTGTTTCTTACTTTTCCCCTGCCGTTTTATTGGATTTTTATCCACGATTCTCCCCGTTTAATCGGCAGGTTTCCGCTTTACGTAAAAGAACCGccc                                                                                      >  2:1077086/1‑239 (MQ=255)
                                                                                                                                                        gtaatcgtaGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGaa                                                                                <  1:1040854/239‑1 (MQ=255)
                                                                                                                                                         taatcgtaGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGc                                                                                                                                                                                                    <  1:549969/122‑1 (MQ=255)
                                                                                                                                                         taatcgtaGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCCGGGTTCGCCTGTACGGAAAAAAACCGGCCCAaaaaa                                                                               >  2:922229/1‑238 (MQ=255)
                                                                                                                                                           atcgtaGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTg                                                                             <  1:947172/239‑1 (MQ=255)
                                                                                                                                                                aGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTAGCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGt                                                                         <  1:819224/238‑1 (MQ=255)
                                                                                                                                                                        ggCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTTTCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCGTTTCGTAAAAGAACCGGCCAAACAAATGCAGAAAAAATGtt                                                                >  2:819224/1‑239 (MQ=255)
                                                                                                                                                                         gCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGttt                                                               <  1:23839/239‑1 (MQ=255)
                                                                                                                                                                            tgttAGGGTTTTCCTTTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTa                                                             <  2:596523/235‑1 (MQ=255)
                                                                                                                                                                             ggTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTa                                                                                                      >  2:240153/1‑196 (MQ=255)
                                                                                                                                                                                    cTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGTGGCAGGTTTCCGCTGTACGTCAAAGAACCGGCCAAAGACTTGCAGTAAATATGttt                                                               >  1:626000/1‑228 (MQ=255)
                                                                                                                                                                                       aCCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATc                                                                                                                                                                                                                    >  2:57166/1‑76 (MQ=255)
                                                                                                                                                                                       aCCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATc                                                                                                                                                                                                                    <  1:57166/76‑1 (MQ=255)
                                                                                                                                                                                       aCCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGt                                                 >  2:209253/1‑239 (MQ=255)
                                                                                                                                                                                       aCCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGt                                                 >  1:911919/1‑239 (MQ=255)
                                                                                                                                                                                        ccTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGtt                                                >  2:912582/1‑239 (MQ=255)
                                                                                                                                                                                             tCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGttcattc                                           >  1:814626/1‑239 (MQ=255)
                                                                                                                                                                                              tttttaCTTAAAAGCAACAGAATTGTAACT‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGttcattca                                          <  2:459214/235‑1 (MQ=255)
                                                                                                                                                                                              cTTATCCATAAAAGCAACAGAATGGTAAAA‑TTTTATCGCGTGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGGCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTGTCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGttcattca                                          <  1:73694/239‑1 (MQ=255)
                                                                                                                                                                                              cTTATACATAAAAGCAACAGAATGGTAACA‑TTTTAACGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGttcattca                                          <  1:22894/239‑1 (MQ=255)
                                                                                                                                                                                               ttATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTcac                                         <  1:209253/239‑1 (MQ=255)
                                                                                                                                                                                                 ataCATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTGCGTAAAAGAACCGGCCAAAGAATTGCAGTAAat                                                                     >  2:962385/1‑209 (MQ=255)
                                                                                                                                                                                                    aaaaaaaACAAAGGAATGGTGACA‑TTTGGTCGCGGGATAGCCAAATGAACCCCGGTATGTATCTGGCTATATCCTGTACGGCCTGTGGTTTATCTTGTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTAATCGACGATGCTCTCCGTTAAAGCGGGAGGTTTCCGCTGTACGTAAAAGAACCGGCAAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACaagaa                                    <  2:4155/236‑4 (MQ=255)
                                                                                                                                                                                                          aGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAg                               <  1:541699/238‑1 (MQ=255)
                                                                                                                                                                                                               cAGAATGGTAACA‑TTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGc                                                                                                                                                                                                                <  2:397111/56‑1 (MQ=255)
                                                                                                                                                                                                               cAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTaa                                                                       <  1:723088/193‑1 (MQ=255)
                                                                                                                                                                                                               cAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTaa                                                                       >  2:723088/1‑193 (MQ=255)
                                                                                                                                                                                                                    tGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTaa                                                                       >  1:186039/1‑188 (MQ=255)
                                                                                                                                                                                                                    tGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTaa                                                                       <  2:186039/188‑1 (MQ=255)
                                                                                                                                                                                                                    tGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTACAAAAAATGGGGAAATCCAgga                     >  2:541699/1‑236 (MQ=255)
                                                                                                                                                                                                                         aaa‑TTTTATCGCGGTTTATTCAATTTATCCCCGTCATTTATCGTGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCa               <  2:679880/237‑1 (MQ=255)
                                                                                                                                                                                                                         aCA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCa               <  2:286124/239‑1 (MQ=255)
                                                                                                                                                                                                                         aCA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCa               <  1:912582/239‑1 (MQ=255)
                                                                                                                                                                                                                         aCA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCa               <  1:162680/239‑1 (MQ=255)
                                                                                                                                                                                                                          actttttATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCa               <  1:479815/236‑1 (MQ=255)
                                                                                                                                                                                                                           a‑TTTTATCGGGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCAtt             <  1:600841/239‑1 (MQ=255)
                                                                                                                                                                                                                           a‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCAtt             <  2:814626/239‑1 (MQ=255)
                                                                                                                                                                                                                              tttATCGGGGGTTAGCCAATTGATCCCCGTTATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATtct           <  2:559756/239‑1 (MQ=255)
                                                                                                                                                                                                                              tttATCCCGGGTTAGCCAATTGTTCCCCCGTATTTTTTTGGCTATTTCCTGATCTGCCTTTGCTTTGGCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATtct           <  2:1079202/239‑1 (MQ=255)
                                                                                                                                                                                                                               tttggggggttAAGCCAATTTGTCCCCTTCATTTTTCTGGCTATTGCCTGAGCGGCTTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATtctc          <  2:872616/229‑1 (MQ=255)
                                                                                                                                                                                                                                   cggcggTAAGCCAATTGATCCCCTGCATTTATCGGGCTGTATCCTTAGCGGCCTTTGCGTTGTCTGTTTCTTAGTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATTCTCTCCGTTTAAGCGACAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGt      <  1:36766/235‑1 (MQ=255)
                                                                                                                                                                                                                                   cgcgGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTg                                                                             >  2:778666/1‑168 (MQ=255)
                                                                                                                                                                                                                                   cgcgGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACATTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTg                                                                             <  1:778666/168‑1 (MQ=255)
                                                                                                                                                                                                                                   cgcgGGTAAGCCAATTGACCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTGGCCCTGACGTTTTATTGGATTTTTATCGACGATACGCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGt      <  1:532823/239‑1 (MQ=255)
                                                                                                                                                                                                                                      gggTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGTCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGTCGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGTtat    <  1:67277/238‑1 (MQ=255)
                                                                                                                                                                                                                                       ggTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTGGCTTTGTCTGTTTCTTACTTTTGCCCTTACGTTTTATTGGCTTTTTATCTACGTTCCTCTCCGTTTAACCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTCATTATGTTTTTTTGGCATACCCTGCGATCACAACACGTGAGCAAACAAATATTTTCATTTCCTGGtaaat  >  2:111968/1‑236 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        
GACGGAGAGCTTTCGACGGGATGGTGCCCCAGTGGGTGCAACCGCCGCCAACATTTTGATAACGCTCGATAACTGCGACGCGCGCACCTTGCTTAACCAGGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTAC‑TATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACA‑TTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAG  >  NC_000913/4160618‑4161085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A