Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I0 R1 140 172.9 3477528 97.9% 3404499 226.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,969,794 A→C 10.7% G397G (GGT→GGG)  tap ← methyl‑accepting protein IV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,969,7940AC10.7% 252.6 / 2.3 85G397G (GGT→GGG) tapmethyl‑accepting protein IV
Reads supporting (aligned to +/- strand):  ref base A (67/5);  new base C (2/10);  total (69/16)
Fisher's exact test for biased strand distribution p-value = 6.32e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTCGCGGCATTTTTTGCCAGTTCCGACG  >  NC_000913/1969557‑1970020
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                  
cATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCa                                                                                                                                                                                                                                    >  2:1504166/1‑238 (MQ=255)
  tCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCCCT‑TCa                                                                                                                                                                                                                                    >  2:337273/1‑236 (MQ=255)
  tCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCa                                                                                                                                                                                                                                    >  2:952044/1‑236 (MQ=255)
    tGGCTGACGCCTTGTCCACCCTCCTAAACCCCCCCGTTGTGTCCTCCCGCCGGAGGGGCATTTTCTCCCATATGGCCGTCCCCGGGGGCCACCAAACTGAAAATCTCGACCATGGTCGGGGCGGCGTTTTCCCCCATTTCCGACCCCGGCTGGACCCGATTGACTGC‑CTCTCCGATCGGCCCTTT‑G‑ATCTCTTTT‑GCTCCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGc                                                                                                                                                                                                                               <  1:764885/239‑1 (MQ=255)
           cAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGCGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑T‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCCCT‑TCACCTGTcaccaca                                                                                                                                                                                                                        >  1:1045208/1‑238 (MQ=255)
                tgtgCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑TGCACT‑TCACCTGCCACTACCGCaaa                                                                                                                                                                                                                   >  2:1308310/1‑239 (MQ=255)
                tgtgCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCaaa                                                                                                                                                                                                                   >  2:369455/1‑239 (MQ=255)
                       ccTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACg                                                                                                                                                                                                             >  2:358605/1‑238 (MQ=255)
                          gCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGcc                                                                                                                                                                                                           >  1:725877/1‑237 (MQ=255)
                            tCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCt                                                                                                                                                                                                        >  1:1082499/1‑238 (MQ=255)
                            tCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCt                                                                                                                                                                                                        >  1:1693531/1‑238 (MQ=255)
                                            gttCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGcc                                                                                                                                                                                       >  2:1706149/1‑239 (MQ=255)
                                             ttcttcCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGcc                                                                                                                                                                                       >  2:285079/1‑238 (MQ=255)
                                                  ccGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTc                                                                                                                                                                                  >  2:373534/1‑238 (MQ=255)
                                                              catttccccccATATTGTCGTCCACGCGTGTCCCCACACTGACAATCTGGTTCGTGTGCGCGGCGGCGTTATTCACCAGTTTCAACCCCTGCTGGACCCGATGGCCTGA‑CTCTTCGACCAGCCTTTT‑G‑CTCTCTTTG‑GCCGCCCGCGCGCAACGGCGGGCAAGATGC‑CGCACT‑CCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt                                                                                                                                                                      <  1:864502/230‑1 (MQ=255)
                                                                   tctcCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAggg                                                                                                                                                                 >  2:1280584/1‑238 (MQ=255)
                                                                   ccccccgaagggtcttcccgcggggCCACCGACCTAACAATCACGATCATGGGCGCGGGGGCTTTTTCCCCCAGTTTCGACCCCTGCTGGACACGTTTAATTGC‑CTTTTCGATCACCCCTTT‑G‑ATCTTTTTT‑GCGGCCGGCGCGTGCCGGCGGGAAAGATGG‑CCCACT‑TCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGc                                                                                                                                                                <  1:951422/217‑1 (MQ=255)
                                                                            aTGTCGTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCCTCCACTGCCGCATTCAGGGCCAGCATAtt                                                                                                                                                       >  1:1664043/1‑239 (MQ=255)
                                                                                 gTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTgg                                                                                                                                                     >  1:694298/1‑236 (MQ=255)
                                                                                  ttCACGCGGGTCACCGAACTGACACTATCGATCATGGTCGCGGCGGCGTTATTCACCAGTATCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACCGCTGGCAAGATTG‑CGCACT‑TCCCCTGCCACTACCGCAAAATCACGCCCCTGCTCTCCCGCGCGCGCCGCTCCCACTGCCCCATTCCGGGCCAGAATATTgg                                                                                                                                                     >  1:1090897/1‑235 (MQ=255)
                                                                                  ttCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCCCTGCCGCATTCAGGGCCAGCATATTGGTCTg                                                                                                                                                 >  1:1208457/1‑239 (MQ=255)
                                                                                  ttCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCCTCCACTGCCGCATTCAGGGCCAGAATATTTGTCTg                                                                                                                                                 >  2:1289486/1‑239 (MQ=255)
                                                                                                     tGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCa                                                                                                                                                                        >  2:1450572/1‑197 (MQ=255)
                                                                                                     tGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATa                                                                                                                              >  2:1174003/1‑239 (MQ=255)
                                                                                                      gACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATa                                                                                                                              >  2:179421/1‑238 (MQ=255)
                                                                                                        caatacctacatgggTCGGGGCGGCGTTTTTCACGATTTTCGACCCGTGCTGGAGCTTTTTAACTGA‑CTCTCCGACCA‑CCCTTT‑GCCTCTTTTTT‑GCTCCGTGCGCGCTACGGCTGCCAAGTTTG‑CGCCCT‑TCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATaa                                                                                                                             <  1:1676689/224‑1 (MQ=21)
                                                                                                              cGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATa                                                                                                                     >  2:738127/1‑239 (MQ=255)
                                                                                                                 tCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATg                                                                                                                  >  1:509811/1‑239 (MQ=255)
                                                                                                                 tCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCAAGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGACAGCAATTCAGTCGATCACGCCGATAATg                                                                                                                  >  1:1349585/1‑239 (MQ=255)
                                                                                                                     ggTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCg                                                                                                               >  2:782323/1‑238 (MQ=255)
                                                                                                                     ggTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCg                                                                                                               >  2:1711473/1‑238 (MQ=255)
                                                                                                                     ggTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCg                                                                                                               >  2:838362/1‑238 (MQ=255)
                                                                                                                      gTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCa                                                                                                                                                                                >  2:416883/1‑172 (MQ=255)
                                                                                                                       tCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGTTAACGCTGATAATGTCGccc                                                                                                            >  1:1388140/1‑238 (MQ=255)
                                                                                                                       tCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGCTAATGTAGCCa                                                                                                            >  2:260618/1‑239 (MQ=255)
                                                                                                                                ctttATTCCCCAGTTCCGACCCCTGCTGGACCCGATTGACTGC‑CTCTCCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTCCCTGCGCGCTGCGGCTGGCAAGTTGG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTg                                                                                                    <  2:608924/236‑1 (MQ=255)
                                                                                                                                 gTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCg                                                                                                  >  2:1693948/1‑239 (MQ=255)
                                                                                                                                    aggatgttcataagagccaggctgGACACGATTGCCTGA‑CTCTTCGACCACCCCTTT‑G‑ACCTCTTTT‑GCGGCCTGCGCGCTGCGGCGGGCAAGTTGC‑CGCCCT‑CCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGc                                                                                               <  1:715142/219‑1 (MQ=37)
                                                                                                                                          cAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTTGTCTGGAAAGCAATTCCGTCGATAACGCTGCTAATTCTCCCAATTTTTTTGTCGCtggtgg                                                                                         >  1:1014071/1‑239 (MQ=255)
                                                                                                                                             tttCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATCACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGctt                                                                                      >  1:785900/1‑237 (MQ=255)
                                                                                                                                             tttCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGa                                                                                      >  2:766848/1‑239 (MQ=255)
                                                                                                                                               tCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGa                                                                                      >  2:954995/1‑237 (MQ=255)
                                                                                                                                               tCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGa                                                                                      >  1:305521/1‑237 (MQ=255)
                                                                                                                                               tCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCCCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCACTTTTTTGCGAGCTGGCGGCGAt                                                                                     >  1:39799/1‑238 (MQ=255)
                                                                                                                                                      cggctggcccgtatggcctaa‑ctCTTCGACCACCCCTTT‑G‑TTCTCTTGT‑GCCCCCGGCGGGC‑AGGGGCGGGAAAATTGCCCCCCT‑TCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCa                                                                             <  1:272672/219‑1 (MQ=25)
                                                                                                                                                      cggctgGACACGATTGACTGC‑CTCTCCGACCAGCCCTGG‑G‑TCCTTTTTT‑GCTGCCGGGCCGCTCCGGCGGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCa                                                                             <  2:884134/237‑1 (MQ=38)
                                                                                                                                                      cggcgcgACACGATTACCGGC‑CTCTCCTATCCCCCTTTG‑G‑ATCTTTTTT‑GCCCCCTGCCCTCTACGCCTGGAAAGTTGC‑CGCTCT‑CCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCa                                                                             <  1:1711473/233‑1 (MQ=25)
                                                                                                                                                       tgctgGACACGCTTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGCCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTg                                                                               >  1:734814/1‑236 (MQ=255)
                                                                                                                                                         ctgGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCa                                                                             >  2:184736/1‑236 (MQ=255)
                                                                                                                                                             ccacGTTTGACTCATCTC‑TCGATC‑GCCCTTTGG‑ATCTTTTTT‑GCTCCCGGCTCGTTCCGCCGGGAAAGTTTC‑CCCCCT‑CCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGtgtg                                                                      <  1:1174003/238‑1 (MQ=25)
                                                                                                                                                              cacGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGa                                                                     >  2:672198/1‑239 (MQ=255)
                                                                                                                                                               acGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAg                                                                    >  2:650825/1‑239 (MQ=255)
                                                                                                                                                                 gATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTc                                                                  >  2:511077/1‑239 (MQ=255)
                                                                                                                                                                 gATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTc                                                                  >  1:648207/1‑239 (MQ=255)
                                                                                                                                                                 gATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGGCGATAACGCTGATAATGTCGCCAATTTTTTCCGACCTGGTGGCGATCCCCTGCATGGTTTGAGt                                                                   >  1:1580298/1‑238 (MQ=255)
                                                                                                                                                                  tttGACTGA‑CTCTTCCATCCGCCCTTT‑C‑TTCTTTTTG‑GCGCCCTGCGCGCCACGGGGGCCAAGTTGG‑CCCACT‑CCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCa                                                                 <  1:1564824/238‑1 (MQ=37)
                                                                                                                                                                   ttttatttatcctTCAATCACCCTTTT‑T‑ATTTCTTTG‑GCGTCCTCGACGCACCGGCTGGCAAGATTG‑CGCACT‑CCGCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCa                                                                 <  2:826239/228‑1 (MQ=37)
                                                                                                                                                                   ttGCCTGA‑CTCTTCGACCACCCTTTT‑G‑TTCTCTGTT‑GCTGCCTGCGCGCTCCGGCGGGAAAGATTC‑CCCCCT‑CCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCa                                                                 <  2:995209/238‑1 (MQ=38)
                                                                                                                                                                   ttGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCAt                                                                >  1:862601/1‑239 (MQ=255)
                                                                                                                                                                   ttGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGAt                                                                                     >  2:715142/1‑218 (MQ=255)
                                                                                                                                                                   ttGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTACCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCa                                                                 <  2:669715/238‑1 (MQ=255)
                                                                                                                                                                      actga‑ctCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAAt                                                                                                                   >  2:436461/1‑185 (MQ=255)
                                                                                                                                                                        ctgctcctccGATCAG‑CCTTT‑T‑TTCTCTTTTGGCTGCCGGCGCGCAACGGCGGGCAAG‑TTT‑CCCACTCCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTa                                                            <  1:411020/230‑1 (MQ=255)
                                                                                                                                                                          ccccctcccaTCAGCCCTTT‑G‑ATCTCTTTT‑GCTCCCTCCGCGCTCCGGCTGCCATGATTG‑CGCTTC‑TCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACt                                                          <  2:621280/230‑1 (MQ=38)
                                                                                                                                                                             tctTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGa                                                        >  2:1714431/1‑238 (MQ=255)
                                                                                                                                                                                 cgttCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTCCGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCACAACCACGCCCCTGCTCTCCGGCCCCACCCCCTTCCACTGCCGCATTCCGAGCCAGAATAATGCTCTGGGAAGCAATTCCGTCGATCACGCTGAAACTGTCGCCAATTTTTTGCGAGCTGGTGGAGATCTTCTGACTGGAGTGCTTCATTGTACTAACATGa                                                   >  1:1112399/4‑239 (MQ=255)
                                                                                                                                                                                     cAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCACAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGCAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAaca                                                >  2:26655/1‑238 (MQ=255)
                                                                                                                                                                                       gCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGCCCTGAACAcc                                              >  2:459191/1‑238 (MQ=255)
                                                                                                                                                                                       gCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACAcc                                              >  2:957677/1‑238 (MQ=255)
                                                                                                                                                                                         ccTTT‑G‑ATCTCTTTT‑GCTGCCTGAGCGCTACGGCTGGCAAGATTT‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGCGCCGCTTCCACTGCCGCATTCAGGGCCAGACTATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATCATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCGCCTGCATGGTGGGAGTCATGGTACTGACCTGAACAccgc                                            >  1:425316/1‑238 (MQ=255)
                                                                                                                                                                                                    tctTTT‑GCTGCCTGCGCGTTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGCGGCGATCTCCTGCATGGTGTGAGGCTTGGTACTGACCTGAACACCGCCCGCCTgcg                                   >  2:1682435/1‑238 (MQ=255)
                                                                                                                                                                                                    tctTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTgcg                                   >  2:1643417/1‑238 (MQ=255)
                                                                                                                                                                                                     ctTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGcc                                 >  2:160759/1‑239 (MQ=255)
                                                                                                                                                                                                        tt‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGtt                              >  2:267926/1‑239 (MQ=255)
                                                                                                                                                                                                            ctgcctgcGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCATTTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAg                                                                                                >  2:333688/1‑170 (MQ=255)
                                                                                                                                                                                                               cctgcGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTcgcg                        >  2:595469/1‑239 (MQ=255)
                                                                                                                                                                                                               cctgcGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTcgcg                        >  2:1163706/1‑239 (MQ=255)
                                                                                                                                                                                                               cctgcGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTcgcg                        >  2:468334/1‑239 (MQ=255)
                                                                                                                                                                                                                 tgcGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTCGCGGc                      >  2:1389563/1‑239 (MQ=255)
                                                                                                                                                                                                                  gcgcgcTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTCGCGGCa                     >  1:1303544/1‑239 (MQ=255)
                                                                                                                                                                                                                   cgcgcTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCGGAATATTGGTCTGGAAAGCCATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGTGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGAc                                                       >  2:682965/1‑204 (MQ=255)
                                                                                                                                                                                                                               ggCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTCGCGGCATTTTTTGCCAGt         >  2:491404/1‑238 (MQ=255)
                                                                                                                                                                                                                               ggCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTCGCGGCATTTTTTGCCAGt         >  2:318864/1‑238 (MQ=255)
                                                                                                                                                                                                                                  aaGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTCGCGGCATTTTTTGCCAGtt        >  2:1584198/1‑236 (MQ=255)
                                                                                                                                                                                                                                       tG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCAT‑GTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTCGCGGCATTTTTTGCCAGTTCCGACg  >  2:345083/1‑236 (MQ=255)
                                                                                                                                                                                                                                        g‑cgcACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTCGCGGCATTTTTTGCCAGTTCCGACg  >  2:1711085/1‑236 (MQ=255)
                                                                                                                                                                                                                                          cgcACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTCGCGGCATTTTTTGCCAGTTCCGACg  >  1:664078/1‑235 (MQ=255)
                                                                                                                                                                                                                                           gcACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTCGCGGCATTTTTTGCCAGTTCCGACg  >  1:617706/1‑234 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                  
CATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGA‑CTCTTCGATCAGCCCTTT‑G‑ATCTCTTTT‑GCTGCCTGCGCGCTACGGCTGGCAAGATTG‑CGCACT‑TCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTCGCGGCATTTTTTGCCAGTTCCGACG  >  NC_000913/1969557‑1970020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A