Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F17 I0 R1	124	155.2	3079696	98.3%	3027341	226.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,536,219	C→A	23.3%	intergenic (+187/+396)	yjhR → / ← nanS	pseudogene, helicase family, putative frameshift suppressor/9‑O‑acetyl N‑acetylneuraminic acid esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,536,219	0	C	A	23.3%	126.2 / 3.7	54	intergenic (+187/+396)	yjhR/nanS	pseudogene, helicase family, putative frameshift suppressor/9‑O‑acetyl N‑acetylneuraminic acid esterase
Reads supporting (aligned to +/- strand): ref base C (15/24); new base A (8/5); total (25/29)
Fisher's exact test for biased strand distribution p-value = 2.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAAAATGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAATCTGTAAGCAATAACA  >  NC_000913/4535981‑4536450

aaaaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACATCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATgcaagc                                                                                                                                                                                                                                              <  2:598411/239‑1 (MQ=255)

  aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATgcaagc‑aa                                                                                                                                                                                                                                           <  1:367037/239‑1 (MQ=255)

  aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATgcaagc‑aa                                                                                                                                                                                                                                           <  2:229236/239‑1 (MQ=255)

  aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATgcaagc‑aa                                                                                                                                                                                                                                           <  2:290610/239‑1 (MQ=255)

  aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATgcaagc‑aa                                                                                                                                                                                                                                           <  1:1478062/239‑1 (MQ=255)

  aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATgcaagc‑aa                                                                                                                                                                                                                                           <  1:1126599/239‑1 (MQ=255)

  aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATgcaagc‑aa                                                                                                                                                                                                                                           <  1:1060263/239‑1 (MQ=255)

  aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGACTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATgcaagc‑aa                                                                                                                                                                                                                                           <  1:110434/239‑1 (MQ=255)

  aaaaTGAAATAAAGGCAATATATGGTAGTATGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATgcaagc‑aa                                                                                                                                                                                                                                           <  2:400633/239‑1 (MQ=255)

            aaaGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AAtaatat‑aata                                                                                                                                                                                                                                <  2:1271/239‑1 (MQ=255)

                              tCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGCTTATAAATCTTATTGTTTACCTATTCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTTTGTTTTG‑AAG‑AAAAAGCAAAC‑AACAAAAT‑AAAAAATGAATAATttttttta                                                                                                                                                                                                              >  2:593972/1‑237 (MQ=255)

                              tCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGTGG‑CTTTTTTGTAAGTA‑AAA‑AAAATAC‑AGC‑ACTAATAT‑ATAAATAGGCTAATTTTTTTgt                                                                                                                                                                                                              >  1:284048/1‑238 (MQ=255)

                              tCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTT‑C‑TTGTTTGAATGA‑AAG‑AAAATATAAAC‑AATAATAT‑AATAAAGGCCGAATTTTTTTgt                                                                                                                                                                                                              >  1:1175373/1‑238 (MQ=255)

                              tCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑T‑TTTTTTTAATTA‑AAT‑AAAAAGTAAGCGAaaaatatcttcttatatactattttac                                                                                                                                                                                                                 >  2:199442/1‑218 (MQ=255)

                              tCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑T‑TTTTTGTTATTA‑AAGTAAAATAC‑AGA‑AAAAATAT‑AATAATAGGCTAATtttttttt                                                                                                                                                                                                              >  2:610994/1‑237 (MQ=255)

                              tCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑G‑TTTTATGTATGT‑AAT‑AAAAAACAAAC‑AATAATAt‑aataaataaccaattttttttt                                                                                                                                                                                                              >  2:247883/1‑222 (MQ=255)

                              tCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGG‑C‑TTTTTGGAATGA‑AAA‑AAAAAACAAGG‑AAATATAt‑aaaaaagtacatattttttttt                                                                                                                                                                                                              >  1:1308699/1‑222 (MQ=255)

                               cTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGaa                                                                                                                                                                                                             <  1:1195851/239‑1 (MQ=255)

                               cTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATAAAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGGCTAATTTCTTTGaa                                                                                                                                                                                                             <  2:178362/239‑1 (MQ=255)

                                tGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCC‑TTTTTGGAAT‑A‑AAA‑AAAAAGAAAAC‑AAACATAT‑TATAAATGCCTTATtttttttttc                                                                                                                                                                                                            >  1:1067348/1‑235 (MQ=255)

                                tGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑G‑TTTTTTGTATTA‑TAA‑AAAACATAAAA‑AATAATAT‑aattatatatttaattttttttaa                                                                                                                                                                                                            >  2:1195851/1‑221 (MQ=255)

                                tGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑G‑TTTTTTGAATGA‑AAG‑AAAAAACAAGA‑AAAAAAAT‑AATAAAGGCCTAATTTTTTTTaaa                                                                                                                                                                                                            >  2:516179/1‑238 (MQ=255)

                                        cGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTTTTTATGT‑AAA‑AAAAAACAATC‑AATAATGA‑AAAAAAGGACTTATTTCtttttacacctctct                                                                                                                                                                                                    >  2:691560/1‑227 (MQ=255)

                                                           gaGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTAt                                                                                                                                                                               <  2:400103/239‑1 (MQ=255)

                                                                 ttCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTTTGTATGA‑AAA‑AAAAAGCAAGC‑AAAAATAT‑AATAATAGGCTCATTTTTTTTAACATTATTAAGTATTATAAT‑ATTGT‑CTAtttttct                                                                                                                                                                         >  1:787183/1‑235 (MQ=255)

                                                                      gATACAGGAGTAGTAAAGGGCATATTGTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCa‑ta                                                                                                                                                                                              <  1:464543/214‑1 (MQ=255)

                                                                         aCAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGt                                                                                                                                                                 <  1:691560/239‑1 (MQ=255)

                                                                           aGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTTGGAATGA‑AAG‑AAAATGGAAGC‑AATAATAT‑AATAATTGCCTAATTTTTTTTAACATCATTTAGCA‑TATCATCATTGT‑CTATTTATCAGAATCCATCg                                                                                                                                                               >  1:396881/1‑239 (MQ=255)

                                                                           aGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGAG‑C‑TTTTTGGAATGA‑AAA‑AAAATCACAGA‑AATAATAT‑AATAAAAAGCTAATTTCTTTTTAAATCATTAAGGA‑AATCATCAATGT‑CTCTTTTCCATAATCCGTcc                                                                                                                                                               >  1:191377/1‑238 (MQ=255)

                                                                             gAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑G‑TTTTTTGCATGA‑AAT‑ATAAAACCATA‑AAAAATAT‑AAAAATAGGATTATTTCTTTTTACATAATTTATAA‑TAATATCATCATCCTTTTTTCCA‑TATCCGTCGaa                                                                                                                                                             >  2:887807/1‑238 (MQ=255)

                                                                                tagtaAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑T‑CTTTTTTAATGATAAA‑AAAAACAAAGC‑AA‑AATAT‑AAAAATAGTCTCATTTCTTTTTACATCTATTAGAA‑AATAATCATCCT‑CCTTTTGcttatacccatcctactc                                                                                                                                                          >  2:464543/1‑222 (MQ=255)

                                                                                tagtaAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTTTGAATGA‑AAG‑AAAAAACAAGA‑AATAAAAT‑AAAAAAAGTCTAATTTTTTTTTAAATCATTAAACA‑AATCATCATCGT‑TTATTTTTCAGAATACGTCTTCTta                                                                                                                                                          >  2:822284/1‑239 (MQ=255)

                                                                                tagtaAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGG‑G‑TTTTTTTGATGT‑AAA‑AAAAAGAAAAC‑AATAAAAT‑AAAAAAGGCCTAATTTTTTTTTAAATAATTAAGAA‑AATAATCAATGT‑TTATTTTTCAGAATCCCGCGACttt                                                                                                                                                          >  2:1390337/1‑238 (MQ=255)

                                                                                  gtaAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGG‑C‑TTTTTTGAATTA‑AAA‑AAAAAAAAAGA‑AATAATAT‑AAAAAAAAGCTAATTTTTTTTTAAAACATTAAACA‑TATAATCATCGT‑CTTTTTTTCCGAATACCTTGACttttt                                                                                                                                                        >  2:1210959/1‑236 (MQ=255)

                                                                                    aaaGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTtat‑a                                                                                                                                                       <  1:1315525/237‑1 (MQ=255)

                                                                                               tttACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACgg                                                                                                                                          <  1:593972/239‑1 (MQ=255)

                                                                                                    cAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCatat                                                                                                                                     <  1:375504/239‑1 (MQ=255)

                                                                                                      aTGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCATATCa                                                                                                                                   <  1:317789/239‑1 (MQ=255)

                                                                                                       tGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑G‑TTTTTTGGATGA‑AAG‑AAAATGAAAGC‑AATAAAAT‑AATAAAGGCCTAATTTTTTTTTACATCATTAAGAA‑TATCATAATCGT‑CTATTTTTCAGAATCCGTGCACTTTT‑AATTCCCCGAAAGGCatatacg                                                                                                                                  >  2:30893/1‑236 (MQ=255)

                                                                                                        gTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTa                                                                                                                                                                                                     <  1:529720/174‑1 (MQ=255)

                                                                                                          tAAGGCGACTTTCCTTTTTATTTCTAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCATATCAGGa                                                                                                                                <  2:450935/238‑1 (MQ=255)

                                                                                                                          tttATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCtg                                                                                                               <  1:1532319/239‑1 (MQ=255)

                                                                                                                                      cGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACg                                                                                                   <  1:785873/239‑1 (MQ=255)

                                                                                                                                                gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTaaa                                                                                         <  1:1447703/239‑1 (MQ=255)

                                                                                                                                                gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTaaa                                                                                         <  1:1390337/239‑1 (MQ=255)

                                                                                                                                                gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTaaa                                                                                         <  1:1210959/239‑1 (MQ=255)

                                                                                                                                                gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTaaa                                                                                         <  1:247883/239‑1 (MQ=255)

                                                                                                                                                gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTaaa                                                                                         <  1:30893/239‑1 (MQ=255)

                                                                                                                                                gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTaaa                                                                                         <  1:822284/239‑1 (MQ=255)

                                                                                                                                                         tCTTATTGTTTACCTAATCATTTTCCTATGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAt‑aataa                                                                                                                                                                                                                               <  1:642527/99‑1 (MQ=255)

                                                                                                                                                             aTTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑G‑TTTTTTGAATTA‑AAA‑AAAAAGTAAGA‑AAAAAAATAAAAAATGGCCTATTTTTTTTTAA‑ATAATTAAATA‑TATAATTATTTT‑ATTTTTTTCCGAATCCGCCGACTCATAAATACCCACAA‑AGCATATCTGGACGGGGCTCCCAACCCGCTGGGCCAAAAAGTTCTCCTAAACCGTGTGGATGtg                                                                            >  2:42365/1‑238 (MQ=255)

                                                                                                                                                                           cATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATgcaagc‑aa                                                                                                                                                                                                                                           <  1:1323397/70‑1 (MQ=255)

                                                                                                                                                                                    ttGCATAATGCGCCTGGGATTAATGGCGGGTG‑G‑TTTTTTTGATGA‑AAA‑AAAAAGAAAGG‑CATAATAT‑AAAAATGGGCTTATTTTTTTTTACATAATTAAGCA‑TATTATCATCGT‑CTATTTGCCTGAAAATCTCCACTTAT‑AATAACCAAAAACGCATATTAAGAAGGTGCGGGCGACGCTGTTGCGCCAAACAGTCCAGCAAAAAGAGTGGAAGGTCGTCGGTTTCCGAGCCCTAATca                                                     >  2:697682/1‑238 (MQ=255)

                                                                                                                                                                                    ttGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTTGGAATGG‑AAA‑AAAATGCAAGC‑AAAAATAT‑AATAAATGCCTAAATTCTTTGAAAATCATTAAACA‑TATCATCATCCT‑CTATTTTTCAGAATCCGTCGACTTTT‑AATCACCCGAACGGCAAATTCGGACGGGGGTGCGGACGCTTTGGCCCCCAACCCTCCCGTAAACTGTGTTGATGTTCGTTGATTTTCGAGCCAAAATct                                                     >  1:1003496/1‑238 (MQ=255)

                                                                                                                                                                                                 cgtgaaTTAATGGCGGGTT‑C‑TTTTAGGAATAA‑AAA‑AAAATACAAGA‑AATAATAA‑AATAAGGGCCTAATTTCTTGAAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGATTCCGTCGACTTAT‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGGGGCCAAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGAt                                        <  1:757557/234‑1 (MQ=255)

                                                                                                                                                                                                 cTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGAt                                        <  1:610994/239‑1 (MQ=255)

                                                                                                                                                                                                 cTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGAA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAA‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCAAAAACTTCCCAGAAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGAt                                        <  1:887807/239‑1 (MQ=255)

                                                                                                                                                                                                 cTGGGATTAATGGCGGGTG‑C‑TTTTAGAAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTCCCGAGCCATAATCGAAAAGGCGCGGAt                                        <  1:516179/239‑1 (MQ=255)

                                                                                                                                                                                                                              aaTGA‑AAA‑AAAATGCAAGA‑AATAATAA‑AATAATGGATTAATTTCTTTCAAATTCATAAAACC‑TATCAACATCGT‑CTAGTGGCCAGACTCCTACGTATTAT‑AAGCGCAAGAACAGCATATCAGGACGGTGTTGCGAACGCTGTGGCCAAAAACGTTCGAGTAAACTGGATAGCTGTTCGTAGATTTCCGAGCCATAAACGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAACCTg              <  2:1067348/238‑1 (MQ=255)

                                                                                                                                                                                                                               aTGA‑AAT‑AAAATGAAAGA‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TTTAATCCTCTT‑TTATTGGCCAGAATCCGTCGCCTTAT‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCAAAAACGTCCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAATCTGTa            <  2:923273/239‑1 (MQ=255)

                                                                                                                                                                                                                                tGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTCATCTGTac           >  2:607856/1‑238 (MQ=255)

                                                                                                                                                                                                                                tGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAATCTGTaa           >  2:500947/1‑239 (MQ=255)

                                                                                                                                                                                                                                          aaTGCAAGA‑AAAAATAT‑AATAATGGCCTATTTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTGGCCAGAATCCGTCGACTTAA‑AATCGCCAGAACGGCATATAAGGCCGGTGCTGCGAACGCTGTGGCCCAAAACTTTCAAGAAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAATCTGTAAGCAATaac   <  2:396881/239‑1 (MQ=255)

                                                                                                                                                                                                                                           aTGCAAGA‑AATAATAT‑AATAATGGCCTATTTCCTTTGAACATCATTAACCA‑TATCATCATCGT‑CTATTGGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCAAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAATCTGTAAGCAATaaca  <  2:787183/239‑1 (MQ=255)

AAAAAATGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTG‑C‑TTTTAGGAATGA‑AAG‑AAAATGCAAGC‑AATAATAT‑AATAATGGCCTAATTTCTTTGAACATCATTAAGCA‑TATCATCATCGT‑CTATTTGCCAGAATCCGTCGACTTAT‑AATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAATCTGTAAGCAATAACA  >  NC_000913/4535981‑4536450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A