Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F110 I2 R1	12	58.9	2036372	94.8%	1930480	142.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,936,308	C→T	V3I (GTA→ATA)	zwf ←	glucose‑6‑phosphate 1‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,936,308	0	C	T	92.7%	117.4 / NA	42	V3I (GTA→ATA)	zwf	glucose‑6‑phosphate 1‑dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (21/17); total (24/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGG  >  NC_000913/1936170‑1936443

gtcccccGATAATCCGGGCGTCCCGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGcc                                                                                                                                     <  2:901678/138‑1 (MQ=255)

      cGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCCGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAACGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCa                                                                                                                               <  2:486178/143‑1 (MQ=255)

        aTAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCCAAAATGCCCCGGTCACAGGCCTGGGCTGTTCGCGGTAGAGCCagggaatc                                                                                                                             >  1:431222/1‑136 (MQ=255)

         tAATCCGGGTGTCCGGGTTGACCTGACCGGCTTTTTCCAGTTGATACAGGGAACGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGCCACAGCCCTATTCTGTTTGCGTTATCGCCATGTCATtc                                                                                                                            <  1:888499/143‑1 (MQ=255)

           aTCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAGTTTACCCCGCGCAAGGTCGCCTTTCGCGCCGACCATGACCATGGCAGAGGCCTGGGCTTTTTGCGTTTCCGCCATGTCATtctc                                                                                                                          >  2:616579/1‑143 (MQ=255)

                 gtgtCCGGGTGGACCTGACCCGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTCCGACGCGCAGGGTCTCCTTTCTCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAg                                                                                                                    <  2:446381/143‑1 (MQ=255)

                    tCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAACATGACCAGGTCACAGGCCTGGGCTGTTTGCGCCACCCCCATCTCGTGCTCCCTCAGtaa                                                                                                                 >  1:205431/1‑141 (MQ=255)

                    tCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTta                                                                                                                 >  1:167642/1‑143 (MQ=255)

                      cGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTtaac                                                                                                               <  1:619668/143‑1 (MQ=255)

                      cGGGCTGAGCTGACCGGCTTTTGCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTtaac                                                                                                               <  1:614527/143‑1 (MQ=255)

                       gggTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTTCGTtagct                                                                                                              >  2:795808/1‑143 (MQ=255)

                                    cGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTGTCGCCATGGCATTCTGCTTGAGTTAACTAGCCCGGGACTTa                                                                                                 >  2:475481/1‑143 (MQ=255)

                                       cTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGCCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGc                                                                                              <  2:567970/143‑1 (MQ=255)

                                        tttttCCAGTGGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGcc                                                                                             <  1:285827/143‑1 (MQ=255)

                                         ttttCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCa                                                                                            <  2:996194/143‑1 (MQ=255)

                                            tCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGCACTTAAGCCAggg                                                                                         >  2:412459/1‑143 (MQ=255)

                                                gTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGGTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGtata                                                                                     >  1:819737/1‑143 (MQ=255)

                                                           gAAGGCAGCAATGTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGCTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAAtttt                                                                          <  2:243112/143‑1 (MQ=255)

                                                            aaGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTc                                                                         <  2:167642/143‑1 (MQ=255)

                                                               gcagcaATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCGGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTa                                                                      >  2:235606/1‑143 (MQ=255)

                                                               gcagcaATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGTTATACTTGTAATTTTCTTa                                                                      >  2:90707/1‑143 (MQ=255)

                                                               gcagcaATTTACGACGCGCAAGGTCGCCTCTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTa                                                                      >  2:653395/1‑143 (MQ=255)

                                                                     aTTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAACTGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTACGCCCGGGTATCCTTGTAATTTTCTTACGGTGc                                                                >  1:556431/1‑143 (MQ=255)

                                                                          cgacgCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGt                                                           >  2:125939/1‑143 (MQ=255)

                                                                                     gtcttcTTTCGCGCCGAAAACGACCAGGTCACAGGCCGGGGCTGCCTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTAc                                                <  2:85073/138‑1 (MQ=255)

                                                                                       cGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGCACTACTATTACGa                                              >  1:775/1‑143 (MQ=255)

                                                                                          cTTTCGCGCCGAAAATGACGAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACCTATTTTACGGGCt                                           >  1:201692/1‑143 (MQ=255)

                                                                                              cgcgCCGAAAATGACCACGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCg                                       <  1:243779/143‑1 (MQ=255)

                                                                                                cgcCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGaa                                     >  1:911926/1‑143 (MQ=255)

                                                                                                cgcCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGaa                                     >  2:677898/1‑143 (MQ=255)

                                                                                                 gcCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCCTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGGAATTTTCTTACGGTGCCCCGTACTGTTTTTACgctatctctaaa                                    >  1:467348/1‑132 (MQ=255)

                                                                                                   cGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAAc                                  >  2:896618/1‑143 (MQ=255)

                                                                                                    gAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACt                                 <  2:447645/143‑1 (MQ=255)

                                                                                                               ggTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAACACTGTAACCGCTTa                      >  1:330716/1‑143 (MQ=255)

                                                                                                                 tgacaGGCCTGGGCTGTTTGCGTTATCTCCATGTCATTCTCCTTCTGTTTACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATc                    <  1:875306/141‑1 (MQ=255)

                                                                                                                 tCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATc                    >  1:243710/1‑143 (MQ=255)

                                                                                                                   acaGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTCTACGAGCTTGCGAAAACTCTAAACGCTTATCCa                  >  1:145022/1‑143 (MQ=255)

                                                                                                                    caGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTCATCCAc                 <  2:638644/143‑1 (MQ=255)

                                                                                                                      ggCCTGGGCTGTTTGCGTTATCGCCATGTCATGCTCCTTAAGTTAACTAGCCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTCTTACGAGCTTTCGAAAACTGTAAACGCTTATCCAccc               <  1:993412/143‑1 (MQ=255)

                                                                                                                                gtgtGCGTTACCGCCAGGTCATCCGCCATAAGAGAACTAACCCGGTCCTTAAGCCAGGGTATACGTGCAATTTTCTCACGGTCCACCGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTAc     <  2:307472/140‑1 (MQ=255)

                                                                                                                                   tGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAGACTGTAAACGCTTATCCACCCGTGTGATTACggg  >  2:175893/1‑143 (MQ=255)

                                                                                                                                   tGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACggg  >  2:175941/1‑143 (MQ=255)

CTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGG  >  NC_000913/1936170‑1936443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A