Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F9 I0 R1 142 103.7 2603848 81.8% 2129947 227.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 458,189 A→G 14.8% D255G (GAT→GGT)  clpX → ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913458,1890AG14.8% 194.1 / 4.3 77D255G (GAT→GGT) clpXATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease
Reads supporting (aligned to +/- strand):  ref base A (9/53);  new base G (10/2);  total (22/55)
Fisher's exact test for biased strand distribution p-value = 6.44e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGA  >  NC_000913/457951‑458425
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               
gggtataaggttctggTCTACAGCGATTAAATCGGCAACATTTCTC‑GTAAGT‑CAGTCAACCGGTTCATTGCCCGAGACATTTCCGGTGAAGGCGTTCAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAATCATCCGCAGCAGGAATTCTTGCAGGTTGATACCACTAAGATCCTGTTTATTTATGGCGGTGCGTTTGCCGGTCT‑GGa                                                                                                                                                                                                                                                 <  2:181137/224‑1 (MQ=255)
 cacaGGCTGGTATTGTCTTCATCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGAt                                                                                                                                                                                                                                                <  2:994742/239‑1 (MQ=255)
   taaaagggaaaggtctcaatccgtGAAATCGACAAGATTTCTC‑GTAAGTACAG‑CAACCCGTCCATTACCCGGGGCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATaa                                                                                                                                                                                                                                              <  1:1015161/216‑1 (MQ=255)
     gggtttttttGTCTACATCGATGAAACCGACCAGATTTTTG‑GCAAGC‑CAGAACACCCGTCCATGACCCGAGGCGTTGCCGGTGAAGGCGTACGGCCGGCCATGGTGGAGCGGATCGAAGGTACGGTAGCTGCTGTTCCACAGCAAGGTGGGCGTAAACATCCGGAGCAGGAATTCTTGCAGGTTCATAACTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAg                                                                                                                                                                                                                                            <  1:995581/231‑1 (MQ=255)
     agagacagttGTCTACATCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAg                                                                                                                                                                                                                                            <  2:606801/231‑1 (MQ=255)
       gTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAg                                                                                                                                                                                                                                            <  2:189820/237‑1 (MQ=255)
               gtctcaaTCGATGAGATCGACATAGTTTCGC‑GAAAGT‑CAGACAACCCGTCCATTTCCCTGGGCGTTTCCGGTGAAGGCGTCCCGCAGGCACTGTTGAAACTGAGCGAAGGTACGGTCGCTGCTGTTCCACCGCAAGGGGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCa                                                                                                                                                                                                                                  <  1:1289911/233‑1 (MQ=255)
               gTCTAAATCGATGAAAGCGACAAGGTTTCGC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGGTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCa                                                                                                                                                                                                                                  <  2:786145/239‑1 (MQ=255)
                 cTACATCGATGAAAGCGACAAGA‑TTCTCGGTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCAc                                                                                                                                                                                                                                 <  2:1244916/238‑1 (MQ=255)
                 cTAAATCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCAcc                                                                                                                                                                                                                                <  2:1123031/239‑1 (MQ=255)
                  tACATCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTTGCGGTGCGGTTTCCGGTCT‑GGTTAAAAGGATTTTTCAccc                                                                                                                                                                                                                               >  2:1235085/1‑238 (MQ=255)
                     aTCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCCGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGGGGGGTTGGCGGGCT‑GGATaaaaagaatttccaacggtg                                                                                                                                                                                                                            >  1:382926/1‑222 (MQ=255)
                     aTCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGCTTTTCTGTCT‑GTCTAAAGTGAGTTTCCAcccgct                                                                                                                                                                                                                            >  2:970279/1‑235 (MQ=255)
                          tGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTTCTGGGCT‑TGGTAAAATGATTTcccctgtctctttt                                                                                                                                                                                                                        >  2:1015161/1‑227 (MQ=255)
                          tGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGGTAAAGAGATTTCCAACCctgtgggaa                                                                                                                                                                                                                       >  2:1139855/1‑234 (MQ=255)
                                                  aGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTGCCGGTCT‑GGGTAAAAGGATTTTCCCCCGTGGAG‑AAAACGGCCCCGGCC‑TTTGTTTTggg                                                                                                                                                                                              >  2:537281/1‑238 (MQ=255)
                                                      caacacaCCCGTCCATTACCCGAGGCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGgcgc                                                                                                                                                                                            <  2:1002558/232‑1 (MQ=255)
                                                        gACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGGCG‑GGTTAAATGGATTTCTCCCCGGGTGG‑CAAACGCACCCGGCC‑TTGGgttttcacagcg                                                                                                                                                                                         >  2:80187/1‑231 (MQ=255)
                                                          cAACCCGTCCATTGCCCGATCCGGTTCCGGTGAAGGCGTACCGCAGGCACGGTTGAACCTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTAT‑GGATAAAGTGATTTCCCTCCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACgg                                                                                                                                                                                       <  2:714740/239‑1 (MQ=255)
                                                                   cATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATaaaa                                                                                                                                                                                                                                            >  2:517912/1‑178 (MQ=255)
                                                                   cATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAg                                                                                                                                                                                                                                            <  1:517912/179‑1 (MQ=255)
                                                                    aTTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGaa                                                                                                                                                                             <  2:949766/239‑1 (MQ=255)
                                                                           gagaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGGTTTCCGGGCT‑GGATAAAGTGAGTTTCACCCAGGTAGAAAACGGGCCCCGCCC‑TTGGTTTTTGCG‑GACGGTcaaacgcaatgctcgc                                                                                                                                                                      >  2:1070363/1‑227 (MQ=255)
                                                                            agaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGTTTTCCGGGCT‑TGGTAAAGTGGTTTTCCCCCCTGGAG‑GAACCGGCCCCCGCA‑TTGGGTTTGGTGCGACGGTTAAACGCAAGTcccgca                                                                                                                                                                     >  2:781949/1‑235 (MQ=255)
                                                                            agaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTTTCGGTCT‑GGGTAAAAGGATTTCCCCCCGTGTAG‑AAACCCGCTCCGGCC‑TTGGTTTTGGCGCGACGGTCAAAGCGAAGTCCGACa                                                                                                                                                                     >  2:203872/1‑239 (MQ=255)
                                                                                        tGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTTTGGCGGTTGGCTTGTCGGGCT‑CGGTCAAGCGGTTTTCCCCCCTGTTG‑GAAACGGCCCCTGCACTTGCTTTGGTCGCGCCGGAAAAAGCaaaagcgacaaacaaacgcgaa                                                                                                                                                          >  1:899939/1‑220 (MQ=255)
                                                                                                           cTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTCTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGt                                                                                                                                      <  2:996759/239‑1 (MQ=255)
                                                                                                             gTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTTCGTTTGCCGGACT‑GGGTAAAGTGCATTTCCACCGTGTAG‑AAACCGGCTCCGGCATTTGGTTTTGG‑GGGCCGGGAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGGCGCTGGCGCCGGTTg                                                                                                                                    >  2:201529/1‑239 (MQ=255)
                                                                                                             gTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGTTTGCCGGTCT‑TGATAAAGGGATTTTCCACCGTGTAG‑AAAACGGCTCCGGCA‑TTGGTTTTTGCGCGGCGGGAAAAGAGAAGGCCGGCAAAACAAACGAAGGCGAGCTGCTGGGCCAGGGTg                                                                                                                                    >  2:686838/1‑239 (MQ=255)
                                                                                                             gTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTTGCGGGCT‑GGATAAAATGATTTTCCCCCGCGTAG‑AAAACAGGCCCGGCA‑TTTGGTTTGGGGCGGCGGTGAAAGCGAAGAGCGCCAAAAAAAGCGAAGGGGGGCTGCTggggggggggg                                                                                                                                    >  1:1196546/1‑230 (MQ=255)
                                                                                                             gTTGAAACTGAACGAAGGTAGGTGAGTGGCTGTTCCACCGTAAGGTGGGCGTAAACAACCCGAGAAGGAATTCTTGGAGGTCGATACCTGTAAGATCCTGTTTATTTGGGGCGGTGCGGTTGCCGGTCT‑GGATAAAGTGAGTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCTCAGGTTg                                                                                                                                    <  1:201529/239‑1 (MQ=255)
                                                                                                              tttgatgggctggggggTTCGGTGGCGTCTGTTCCCCCGAAAGGTGGGCCTAAAACTCCGCAGCAGGAATTCTTGCAGGTTGATTCCTCTTAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑TGATAAAGTGATTTCCCCCCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGa                                                                                                                                   <  1:760013/224‑1 (MQ=255)
                                                                                                              ttGAAACTGATCGAGGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAGCATCCGCAGCAGGAATGCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGa                                                                                                                                   <  2:197806/239‑1 (MQ=255)
                                                                                                              ttGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGTTTGCCGGGCT‑GGGTAAAATGATTTTCCACCGTGTCG‑AAAACCGCCCCGGCA‑TTGGGTTTGGTGCGCCGGGAAAAGAGAAGACCGGCAAAGCAAGCGAAGGCGGCCTGCTGTGGCCGGTTGa                                                                                                                                   >  2:1074885/1‑239 (MQ=255)
                                                                                                              ttGAAACTGACTGAAGGTGCGTGGTCTGCTGTGCCACCGCACGGGTGGCGTTAACATCCGCATCAGGAATTCGTGAGGGTTGATACCGTTAAGCTCCTGTTGATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGGGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGa                                                                                                                                   <  1:781949/239‑1 (MQ=255)
                                                                                                                   ccTGATCGAAGGGACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCgg                                                                                                                              <  2:170455/238‑1 (MQ=255)
                                                                                                                        tCGAAGGTACGGTAGCTGCGGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGAt                                                                                                                         <  2:537719/239‑1 (MQ=255)
                                                                                                                         cGAAGGTTCGGTAGGTGCTGTCCCCCCGCAAGGTTGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGAt                                                                                                                         <  2:511365/238‑1 (MQ=255)
                                                                                                                             tgttcggtggttgtttttCCCCCGCATGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTTTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGAt                                                                                                                    <  1:537281/223‑1 (MQ=255)
                                                                                                                               tACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGgcg                                                                                                                                           <  2:922143/214‑1 (MQ=255)
                                                                                                                                 cGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGACCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGGATAAA‑TGATTTCTCCCCGTGTAG‑AAAACGTCTCCGGCG‑TTGGGTTTGGAGCGCGGGCAAAATAGAAAGCCGGCAAAGAAAGCGCAAGCGGGCGGGTGGGGCAGAGTGAAAAGGaaaaaatgaacaaa                                                                                                                >  1:27905/1‑227 (MQ=255)
                                                                                                                                   gtcgctcttgTTCCCCCGCAATTGTGGCGTAAAATTCCGTAGCAGGAATTCTTGACGGTTGATACCTCTAAGGTCCTGTTGTTTTGTGGCGGTGCGTTTGCCGGTCG‑GGATAAAGTGATTTCCCACCGGTTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGGGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt                                                                                                              <  1:644447/231‑1 (MQ=255)
                                                                                                                                    tAGCTGCTGTTCCACCGGAAGGTGGGCGTAAACCTCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt                                                                                                              <  2:1094244/238‑1 (MQ=255)
                                                                                                                                    tAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACCTCCGCAGCAGGAATTCTTGCAGGTTTATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt                                                                                                              <  2:1205243/238‑1 (MQ=255)
                                                                                                                                     ggctgcgGTTCCCCCTGAAGGGGGGGGTAAACATCCGAAGCAGGAATTCTGTCAGGTTGATACCTCGAAGATCCTGTTTAGTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGttt                                                                                                             <  1:834255/237‑1 (MQ=255)
                                                                                                                                         gctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGGTAAAGTg                                                                                                                                                                                                                                          >  2:32436/1‑111 (MQ=255)
                                                                                                                                         gctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTg                                                                                                                                                                                                                                          <  1:32436/111‑1 (MQ=255)
                                                                                                                                         gctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCt                                                                                                        <  2:1196546/239‑1 (MQ=255)
                                                                                                                                               ccACCGGAATGTGGGCTGAACCATCCGCAGCAGGAAATCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATccc                                                                                                  <  2:870587/239‑1 (MQ=255)
                                                                                                                                               ccACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATccc                                                                                                  <  2:568557/239‑1 (MQ=255)
                                                                                                                                                cccctgaaGGTGGGTATAAACCTCCGGAGCAGTAATTCTTGCAGTTTCGTCCCTCTAAGAGCCTGTTTGTTTGTGGCGGCGCGTTTGGCGGGCC‑GGATAAAGTGAGTGCCCACCGTGTAG‑AAACCGGCTTCTGCC‑TTTGTTTGGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGACGAGCTGCTGGCGCAGGTTGAACCGGAATATCTGATCAAGTTTGGTCTTATCCCt                                                                                                 <  1:313096/233‑1 (MQ=255)
                                                                                                                                                ccccGTAAGGTGGGCTTAACCATCCGGAGCAGGAATTCTTGCAGGTTGATTCCTCTAAGATCCTGTCTATTTGTGGCGGTGCGGTGTCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCt                                                                                                 <  1:260982/237‑1 (MQ=255)
                                                                                                                                                 gccGCAAGCGGGGCGTAAACATCCGGAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCAGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTg                                                                                                <  2:177259/238‑1 (MQ=255)
                                                                                                                                                  ccGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt                                                                                                              <  2:539900/224‑1 (MQ=255)
                                                                                                                                                                              gggAATTTTTGCAGGTTGATACCTCTAAGATCATGTTTAATTGGTGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCa                                                                   <  2:958429/238‑1 (MQ=255)
                                                                                                                                                                              agGAATTCTTGCAGGTTGATTCCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCg                                                                     <  2:382926/237‑1 (MQ=255)
                                                                                                                                                                                 aaTTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATATAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAAc                                                                 <  2:983329/238‑1 (MQ=255)
                                                                                                                                                                                 aaTTCTTGAAGGTTGATTCCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCCCCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGATAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCa                                                                   <  2:673463/236‑1 (MQ=255)
                                                                                                                                                                                      ttGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGgcg                                                                                                                                           <  2:1208849/159‑1 (MQ=255)
                                                                                                                                                                                           ggTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTGCCGGTCT‑GGATAAAAGGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGGTTTTGCGCGACGGTAAAAGCGAAGTCCGCCAAAACAAGCGAAGGCGAGCGGCTGGCGCAGGGTGCACCGGGAGCACCGAGACAGCTTGGTACTCTCCCTGAGTTGTTTTGGCTTTTTCCGGTTGtgcggacagtgtttgaaa                                                      >  1:1121084/1‑222 (MQ=255)
                                                                                                                                                                                             ttGATACCTCTAAGGTCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTtgaatgaa                                                       <  2:823613/236‑1 (MQ=255)
                                                                                                                                                                                                    ctctAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTc                                                                                                         <  2:168042/179‑1 (MQ=255)
                                                                                                                                                                                                    ctctAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑gaag                                            <  2:857284/239‑1 (MQ=255)
                                                                                                                                                                                                     tctAAGGTCCTGTTTATTTGGGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑gaaga                                           <  2:974074/239‑1 (MQ=255)
                                                                                                                                                                                                           aTCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGa                                                                                                                                                                                                                                                 <  2:942758/38‑1 (MQ=255)
                                                                                                                                                                                                           aTCATGTTTATTGGGTGCGGTGCGTTTGCCGGTCT‑GGATAAAGGTATTACCGACCGTGTAG‑AAACCGGCTACGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGctct                                     <  2:435958/239‑1 (MQ=255)
                                                                                                                                                                                                              cTGTTTATTTGTGGCGGTGCGTTTTCCGGTCT‑GGGTAAAGTGATTTTCCACCGTGTAG‑AAAACGGCTCCGGCA‑TTGGGTTTGGCGCGACGGGAAAAACGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGGCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTGTTGGCAACGTTGAATTAACTGAGC‑GAGGAAGCTCTGAt                                  >  2:326541/1‑239 (MQ=255)
                                                                                                                                                                                                               tGTTTATTTGGGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGAtt                                 <  2:1105375/239‑1 (MQ=255)
                                                                                                                                                                                                                 tttATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCa                               <  2:910734/239‑1 (MQ=255)
                                                                                                                                                                                                                 tttATTTGGGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCa                               <  2:1156287/239‑1 (MQ=255)
                                                                                                                                                                                                                         tgGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGCTTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGGCGGACGGGAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATATGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCa                       <  1:203872/239‑1 (MQ=255)
                                                                                                                                                                                                                         ggggggTGCGTTTGGCGGTCG‑GGAGAAAGGGAGTTCCCACGGTGTAG‑AAACCGGCTCAGGCA‑TTGGGTTTGGCCCGGGCGTAAAAGCGAAGTCCGAAAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTTTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCa                       <  1:1074885/235‑1 (MQ=255)
                                                                                                                                                                                                                           ggggTGCTTTTTCTGGGCT‑GAGTAAAGGTATTTCACACCGTGTAG‑AATCCGGCTTGGGCA‑TTGGTGTCGGCGCGACGGTAAAAGCGAAGTCCGTCAAAGCAAGCGAAGGTGAGATGCTGTCGCAGGTTGCACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGGTGAATGAACTGAGC‑GAAGAAGCGCTGATTCGGACCATCaaa                     <  1:210137/237‑1 (MQ=255)
                                                                                                                                                                                                                             ggggtgttttgcggtttttgATAAAGGGATTGCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TGGGTTTTGGCGCGCATGTAAAAGCGAAGGCCGACAAAGCAAGCGAAGGCGAGCTTCTGGCTCAGGTTGAACCTGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCaaa                     <  1:686838/219‑1 (MQ=255)
                                                                                                                                                                                                                                         ggTCT‑GGGTAAAGTGATTACCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGc       <  2:260512/239‑1 (MQ=255)
                                                                                                                                                                                                                                           tCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAACGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGAGCAAGTTTGGTCTTATCCCTTAGTTTATTGGTCTACTTCCGGTTGTTGCCAAGTTGCAGCAACTGAGC‑GAAGAAGCTCTGATTCACATCCTAAAAGAGCCGCAAACCGcca     >  1:364237/1‑238 (MQ=255)
                                                                                                                                                                                                                                            cT‑GGAAAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGccc     <  2:1093492/238‑1 (MQ=255)
                                                                                                                                                                                                                                             t‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGCAGGCGAGCTGCTGGCGCAGGGTGAACCGGAAGATCTGATCAAGTTTGGGCTAATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAGTGAAAT‑AGCGGAATAAGCCCGGGTTCAGAACCCAAAACAGACAAAAAACGcccaa   >  1:685890/1‑237 (MQ=255)
                                                                                                                                                                                                                                               ggATAAAGTGGTTTCCCTCCGGGTAG‑AAAGCGGGTCCGGCA‑TTGGTTTTGGCGCGTCGGTAAAAGCGAAGTGCGAAAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGACGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACTCCCTGa  <  2:428283/239‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               
GCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGA  >  NC_000913/457951‑458425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A