Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F9 I0 R1	142	103.7	2603848	81.8%	2129947	227.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	458,195	T→G	13.4%	V257G (GTG→GGG)	clpX →	ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	458,195	0	T	G	13.4%	166.0 / 2.0	71	V257G (GTG→GGG)	clpX	ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease
Reads supporting (aligned to +/- strand): ref base T (12/46); new base G (6/5); total (20/51)
Fisher's exact test for biased strand distribution p-value = 2.88e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCTACATCGATGAAATCGACAAGATTTCTC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAG  >  NC_000913/457966‑458430

gtctcaaTCGATGAGATCGACATAGTTTCGC‑GAAAGTCAGACAACCCGTCCATTTCCCTGGGCGTTTCCGGTGAAGGCGTCCCGCAGGCACTGTTGAAACTGAGCGAAGGTACGGTCGCTGCTGTTCCACCGCAAGGGGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCa                                                                                                                                                                                                                                       <  1:1289911/233‑1 (MQ=255)

gTCTAAATCGATGAAAGCGACAAGGTTTCGC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGGTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCa                                                                                                                                                                                                                                       <  2:786145/239‑1 (MQ=255)

  cTACATCGATGAAAGCGACAAGA‑TTCTCGGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCAc                                                                                                                                                                                                                                      <  2:1244916/238‑1 (MQ=255)

  cTAAATCGATGAAATCGACAAGATTTCTC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCAcc                                                                                                                                                                                                                                     <  2:1123031/239‑1 (MQ=255)

   tACATCGATGAAATCGACAAGATTTCTC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTTGCGGTGCGGTTTCCGGTCT‑GGTTAAAAGGATTTTTCAccc                                                                                                                                                                                                                                    >  2:1235085/1‑238 (MQ=255)

      aTCGATGAAATCGACAAGATTTCTC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGCTTTTCTGTCT‑GTCTAAAGTGAGTTTCCAcccgct                                                                                                                                                                                                                                 >  2:970279/1‑235 (MQ=255)

           tGAAATCGACAAGATTTCTC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTTCTGGGCT‑TGGTAAAATGATTTcccctgtctctttt                                                                                                                                                                                                                             >  2:1015161/1‑227 (MQ=255)

           tGAAATCGACAAGATTTCTC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGGTAAAGAGATTTCCAACCctgtgggaa                                                                                                                                                                                                                            >  2:1139855/1‑234 (MQ=255)

                                   aGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTGCCGGTCT‑GGGTAAAAGGATTTTCCCCCGTGGAG‑AAAACGGCCCCGGCC‑TTTGTTTTggg                                                                                                                                                                                                   >  2:537281/1‑238 (MQ=255)

                                      caacacaCCCGTCCATTACCCGAGGCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGgcgc                                                                                                                                                                                                 <  2:1002558/232‑1 (MQ=255)

                                        gACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGGCG‑GGTTAAATGGATTTCTCCCCGGGTGG‑CAAACGCACCCGGCC‑TTGGgttttcacagcg                                                                                                                                                                                              >  2:80187/1‑231 (MQ=255)

                                          cAACCCGTCCATTGCCCGATCCGGTTCCGGTGAAGGCGTACCGCAGGCACGGTTGAACCTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTAT‑GGATAAAGTGATTTCCCTCCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACgg                                                                                                                                                                                            <  2:714740/239‑1 (MQ=255)

                                                    aTTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGaa                                                                                                                                                                                  <  2:949766/239‑1 (MQ=255)

                                                           gagaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGGTTTCCGGGCT‑GGATAAAGTGAGTTTCACCCAGGTAGAAAACGGGCCCCGCCC‑TTGGTTTTTGCG‑GACGGTcaaacgcaatgctcgc                                                                                                                                                                           >  2:1070363/1‑227 (MQ=255)

                                                            agaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGTTTTCCGGGCT‑TGGTAAAGTGGTTTTCCCCCCTGGAG‑GAACCGGCCCCCGCA‑TTGGGTTTGGTGCGACGGTTAAACGCAAGTcccgca                                                                                                                                                                          >  2:781949/1‑235 (MQ=255)

                                                            agaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTTTCGGTCT‑GGGTAAAAGGATTTCCCCCCGTGTAG‑AAACCCGCTCCGGCC‑TTGGTTTTGGCGCGACGGTCAAAGCGAAGTCCGACa                                                                                                                                                                          >  2:203872/1‑239 (MQ=255)

                                                                        tGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTTTGGCGGTTGGCTTGTCGGGCT‑CGGTCAAGCGGTTTTCCCCCCTGTTG‑GAAACGGCCCCTGCACTTGCTTTGGTCGCGCCGGAAAAAGCaaaagcgacaaacaaacgcgaa                                                                                                                                                               >  1:899939/1‑220 (MQ=255)

                                                                                           cTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTCTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGt                                                                                                                                           <  2:996759/239‑1 (MQ=255)

                                                                                             gTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTTCGTTTGCCGGACT‑GGGTAAAGTGCATTTCCACCGTGTAG‑AAACCGGCTCCGGCATTTGGTTTTGG‑GGGCCGGGAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGGCGCTGGCGCCGGTTg                                                                                                                                         >  2:201529/1‑239 (MQ=255)

                                                                                             gTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGTTTGCCGGTCT‑TGATAAAGGGATTTTCCACCGTGTAG‑AAAACGGCTCCGGCA‑TTGGTTTTTGCGCGGCGGGAAAAGAGAAGGCCGGCAAAACAAACGAAGGCGAGCTGCTGGGCCAGGGTg                                                                                                                                         >  2:686838/1‑239 (MQ=255)

                                                                                             gTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTTGCGGGCT‑GGATAAAATGATTTTCCCCCGCGTAG‑AAAACAGGCCCGGCA‑TTTGGTTTGGGGCGGCGGTGAAAGCGAAGAGCGCCAAAAAAAGCGAAGGGGGGCTGCTggggggggggg                                                                                                                                         >  1:1196546/1‑230 (MQ=255)

                                                                                             gTTGAAACTGAACGAAGGTAGGTGAGTGGCTGTTCCACCGTAAGGTGGGCGTAAACAACCCGAGAAGGAATTCTTGGAGGTCGATACCTGTAAGATCCTGTTTATTTGGGGCGGTGCGGTTGCCGGTCT‑GGATAAAGTGAGTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCTCAGGTTg                                                                                                                                         <  1:201529/239‑1 (MQ=255)

                                                                                              tttgatgggctggggggTTCGGTGGCGTCTGTTCCCCCGAAAGGTGGGCCTAAAACTCCGCAGCAGGAATTCTTGCAGGTTGATTCCTCTTAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑TGATAAAGTGATTTCCCCCCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGa                                                                                                                                        <  1:760013/224‑1 (MQ=255)

                                                                                              ttGAAACTGATCGAGGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAGCATCCGCAGCAGGAATGCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGa                                                                                                                                        <  2:197806/239‑1 (MQ=255)

                                                                                              ttGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGTTTGCCGGGCT‑GGGTAAAATGATTTTCCACCGTGTCG‑AAAACCGCCCCGGCA‑TTGGGTTTGGTGCGCCGGGAAAAGAGAAGACCGGCAAAGCAAGCGAAGGCGGCCTGCTGTGGCCGGTTGa                                                                                                                                        >  2:1074885/1‑239 (MQ=255)

                                                                                              ttGAAACTGACTGAAGGTGCGTGGTCTGCTGTGCCACCGCACGGGTGGCGTTAACATCCGCATCAGGAATTCGTGAGGGTTGATACCGTTAAGCTCCTGTTGATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGGGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGa                                                                                                                                        <  1:781949/239‑1 (MQ=255)

                                                                                                   ccTGATCGAAGGGACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCgg                                                                                                                                   <  2:170455/238‑1 (MQ=255)

                                                                                                        tCGAAGGTACGGTAGCTGCGGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGAt                                                                                                                              <  2:537719/239‑1 (MQ=255)

                                                                                                         cGAAGGTTCGGTAGGTGCTGTCCCCCCGCAAGGTTGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGAt                                                                                                                              <  2:511365/238‑1 (MQ=255)

                                                                                                             tgttcggtggttgtttttCCCCCGCATGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTTTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGAt                                                                                                                         <  1:537281/223‑1 (MQ=255)

                                                                                                               tACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGgcg                                                                                                                                                <  2:922143/214‑1 (MQ=255)

                                                                                                                 cGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGACCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGGATAAA‑TGATTTCTCCCCGTGTAG‑AAAACGTCTCCGGCG‑TTGGGTTTGGAGCGCGGGCAAAATAGAAAGCCGGCAAAGAAAGCGCAAGCGGGCGGGTGGGGCAGAGTGAAAAGGaaaaaatgaacaaa                                                                                                                     >  1:27905/1‑227 (MQ=255)

                                                                                                                   gtcgctcttgTTCCCCCGCAATTGTGGCGTAAAATTCCGTAGCAGGAATTCTTGACGGTTGATACCTCTAAGGTCCTGTTGTTTTGTGGCGGTGCGTTTGCCGGTCG‑GGATAAAGTGATTTCCCACCGGTTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGGGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt                                                                                                                   <  1:644447/231‑1 (MQ=255)

                                                                                                                    tAGCTGCTGTTCCACCGGAAGGTGGGCGTAAACCTCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt                                                                                                                   <  2:1094244/238‑1 (MQ=255)

                                                                                                                    tAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACCTCCGCAGCAGGAATTCTTGCAGGTTTATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt                                                                                                                   <  2:1205243/238‑1 (MQ=255)

                                                                                                                     ggctgcgGTTCCCCCTGAAGGGGGGGGTAAACATCCGAAGCAGGAATTCTGTCAGGTTGATACCTCGAAGATCCTGTTTAGTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGttt                                                                                                                  <  1:834255/237‑1 (MQ=255)

                                                                                                                         gctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGGTAAAGTg                                                                                                                                                                                                                                               >  2:32436/1‑111 (MQ=255)

                                                                                                                         gctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTg                                                                                                                                                                                                                                               <  1:32436/111‑1 (MQ=255)

                                                                                                                         gctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCt                                                                                                             <  2:1196546/239‑1 (MQ=255)

                                                                                                                               ccACCGGAATGTGGGCTGAACCATCCGCAGCAGGAAATCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATccc                                                                                                       <  2:870587/239‑1 (MQ=255)

                                                                                                                               ccACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATccc                                                                                                       <  2:568557/239‑1 (MQ=255)

                                                                                                                                cccctgaaGGTGGGTATAAACCTCCGGAGCAGTAATTCTTGCAGTTTCGTCCCTCTAAGAGCCTGTTTGTTTGTGGCGGCGCGTTTGGCGGGCC‑GGATAAAGTGAGTGCCCACCGTGTAG‑AAACCGGCTTCTGCC‑TTTGTTTGGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGACGAGCTGCTGGCGCAGGTTGAACCGGAATATCTGATCAAGTTTGGTCTTATCCCt                                                                                                      <  1:313096/233‑1 (MQ=255)

                                                                                                                                ccccGTAAGGTGGGCTTAACCATCCGGAGCAGGAATTCTTGCAGGTTGATTCCTCTAAGATCCTGTCTATTTGTGGCGGTGCGGTGTCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCt                                                                                                      <  1:260982/237‑1 (MQ=255)

                                                                                                                                 gccGCAAGCGGGGCGTAAACATCCGGAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCAGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTg                                                                                                     <  2:177259/238‑1 (MQ=255)

                                                                                                                                  ccGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt                                                                                                                   <  2:539900/224‑1 (MQ=255)

                                                                                                                                                              gggAATTTTTGCAGGTTGATACCTCTAAGATCATGTTTAATTGGTGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCa                                                                        <  2:958429/238‑1 (MQ=255)

                                                                                                                                                              agGAATTCTTGCAGGTTGATTCCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCg                                                                          <  2:382926/237‑1 (MQ=255)

                                                                                                                                                                 aaTTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATATAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAAc                                                                      <  2:983329/238‑1 (MQ=255)

                                                                                                                                                                 aaTTCTTGAAGGTTGATTCCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCCCCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGATAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCa                                                                        <  2:673463/236‑1 (MQ=255)

                                                                                                                                                                      ttGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGgcg                                                                                                                                                <  2:1208849/159‑1 (MQ=255)

                                                                                                                                                                           ggTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTGCCGGTCT‑GGATAAAAGGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGGTTTTGCGCGACGGTAAAAGCGAAGTCCGCCAAAACAAGCGAAGGCGAGCGGCTGGCGCAGGGTGCACCGGGAGCACCGAGACAGCTTGGTACTCTCCCTGAGTTGTTTTGGCTTTTTCCGGTTGtgcggacagtgtttgaaa                                                           >  1:1121084/1‑222 (MQ=255)

                                                                                                                                                                             ttGATACCTCTAAGGTCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTtgaatgaa                                                            <  2:823613/236‑1 (MQ=255)

                                                                                                                                                                                    ctctAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTc                                                                                                              <  2:168042/179‑1 (MQ=255)

                                                                                                                                                                                    ctctAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑gaag                                                 <  2:857284/239‑1 (MQ=255)

                                                                                                                                                                                     tctAAGGTCCTGTTTATTTGGGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑gaaga                                                <  2:974074/239‑1 (MQ=255)

                                                                                                                                                                                           aTCATGTTTATTGGGTGCGGTGCGTTTGCCGGTCT‑GGATAAAGGTATTACCGACCGTGTAG‑AAACCGGCTACGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGctct                                          <  2:435958/239‑1 (MQ=255)

                                                                                                                                                                                              cTGTTTATTTGTGGCGGTGCGTTTTCCGGTCT‑GGGTAAAGTGATTTTCCACCGTGTAG‑AAAACGGCTCCGGCA‑TTGGGTTTGGCGCGACGGGAAAAACGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGGCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTGTTGGCAACGTTGAATTAACTGAGC‑GAGGAAGCTCTGAt                                       >  2:326541/1‑239 (MQ=255)

                                                                                                                                                                                               tGTTTATTTGGGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGAtt                                      <  2:1105375/239‑1 (MQ=255)

                                                                                                                                                                                                 tttATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCa                                    <  2:910734/239‑1 (MQ=255)

                                                                                                                                                                                                 tttATTTGGGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCa                                    <  2:1156287/239‑1 (MQ=255)

                                                                                                                                                                                                         tgGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGCTTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGGCGGACGGGAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATATGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCa                            <  1:203872/239‑1 (MQ=255)

                                                                                                                                                                                                         ggggggTGCGTTTGGCGGTCG‑GGAGAAAGGGAGTTCCCACGGTGTAG‑AAACCGGCTCAGGCA‑TTGGGTTTGGCCCGGGCGTAAAAGCGAAGTCCGAAAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTTTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCa                            <  1:1074885/235‑1 (MQ=255)

                                                                                                                                                                                                           ggggTGCTTTTTCTGGGCT‑GAGTAAAGGTATTTCACACCGTGTAG‑AATCCGGCTTGGGCA‑TTGGTGTCGGCGCGACGGTAAAAGCGAAGTCCGTCAAAGCAAGCGAAGGTGAGATGCTGTCGCAGGTTGCACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGGTGAATGAACTGAGC‑GAAGAAGCGCTGATTCGGACCATCaaa                          <  1:210137/237‑1 (MQ=255)

                                                                                                                                                                                                             ggggtgttttgcggtttttgATAAAGGGATTGCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TGGGTTTTGGCGCGCATGTAAAAGCGAAGGCCGACAAAGCAAGCGAAGGCGAGCTTCTGGCTCAGGTTGAACCTGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCaaa                          <  1:686838/219‑1 (MQ=255)

                                                                                                                                                                                                                         ggTCT‑GGGTAAAGTGATTACCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGc            <  2:260512/239‑1 (MQ=255)

                                                                                                                                                                                                                           tCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAACGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGAGCAAGTTTGGTCTTATCCCTTAGTTTATTGGTCTACTTCCGGTTGTTGCCAAGTTGCAGCAACTGAGC‑GAAGAAGCTCTGATTCACATCCTAAAAGAGCCGCAAACCGcca          >  1:364237/1‑238 (MQ=255)

                                                                                                                                                                                                                            cT‑GGAAAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGccc          <  2:1093492/238‑1 (MQ=255)

                                                                                                                                                                                                                             t‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGCAGGCGAGCTGCTGGCGCAGGGTGAACCGGAAGATCTGATCAAGTTTGGGCTAATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAGTGAAAT‑AGCGGAATAAGCCCGGGTTCAGAACCCAAAACAGACAAAAAACGcccaa        >  1:685890/1‑237 (MQ=255)

                                                                                                                                                                                                                               ggATAAAGTGGTTTCCCTCCGGGTAG‑AAAGCGGGTCCGGCA‑TTGGTTTTGGCGCGTCGGTAAAAGCGAAGTGCGAAAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGACGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACTCCCTGa       <  2:428283/239‑1 (MQ=255)

                                                                                                                                                                                                                                    aaGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAg  <  2:933306/239‑1 (MQ=255)

                                                                                                                                                                                                                                    aaGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTGTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAg  <  2:1066597/239‑1 (MQ=255)

GTCTACATCGATGAAATCGACAAGATTTCTC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAG  >  NC_000913/457966‑458430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A