Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F9 I0 R1	142	103.7	2603848	81.8%	2129947	227.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	467,118	T→C	16.7%	G65G (GGA→GGG)	ybaE ←	putative ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	467,118	0	T	C	16.7%	222.9 / 3.9	95	G65G (GGA→GGG)	ybaE	putative ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base T (59/15); new base C (2/17); total (61/34)
Fisher's exact test for biased strand distribution p-value = 3.70e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCG  >  NC_000913/466891‑467356

ccGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑c                                                                                                                                                                                                                                         <  2:721357/238‑1 (MQ=255)

                      gCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCa                                                                                                                                                                                                                  >  2:330212/1‑238 (MQ=255)

                            cGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAACGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGCCTTCGCCTGCCACTcccg                                                                                                                                                                                                            >  2:692348/1‑236 (MQ=255)

                                cGGGCGATAGTAGGGAATACCAAACGTGGGTTTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCAt                                                                                                                                                                                                        <  2:1082342/238‑1 (MQ=255)

                                             ggAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGACAGCGA‑AATTGTCCGCGCTTT‑CCC‑GGTCCCGACCTCGCCTGCCA‑TCCACCCacacccccccccctgg                                                                                                                                                                                          >  1:174459/1‑223 (MQ=255)

                                             ggAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATGTCGTAGCGATTCCGGCGGGACCAGAAAGCGT‑AATTTTCCGCGCTTT‑CCT‑CGTCCTGCCTGCGCCCGCCACCCCAGCCACCCACCCCCCtgtc                                                                                                                                                                                           >  1:1105700/1‑237 (MQ=255)

                                               aaTACGCAACGAGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACCGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCCGCACCTCTTGCTGCCTTCCGGTTTCCAGT‑GCCTGTTCCATCCTCGCATTGCTTCGCGATTCCGGCGCGGCCAGAAAGCGC‑AATTGCCCACGCTTT‑ACC‑CGCCCGGACTACCCCGGCCACTCCGACCACCCAGcccccttggccg                                                                                                                                                                                        >  1:199961/1‑233 (MQ=255)

                                                 tACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATACACGGCTGTAACAGAGTGCCCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCCGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCCGCGTGCCCAGAAAGCGT‑AATTTCCCCCGCCTT‑CCC‑CACCCTCTCTGTGCCTCCACCCCCACCCCACCCCCCCCCTGTCCCTg                                                                                                                                                                                       >  1:239638/1‑238 (MQ=255)

                                                 tACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑ACTTGTACGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCCGCCCTCCCGCCTCCTGTCCCTg                                                                                                                                                                                       >  2:698763/1‑238 (MQ=255)

                                                 tACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTg                                                                                                                                                                                       >  2:855089/1‑238 (MQ=255)

                                                 tACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTg                                                                                                                                                                                       >  2:153350/1‑238 (MQ=255)

                                                 tACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCCGCCTTT‑CCC‑CGTCCTGCCCACGCCTGCCACCCCCGCCAACCCCCCCCCTGGGcccg                                                                                                                                                                                       >  1:537137/1‑236 (MQ=255)

                                                    gCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGCCGc                                                                                                                                                                                   >  2:372550/1‑239 (MQ=255)

                                                    gCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGc                                                                                                                                                                                   >  2:1018924/1‑239 (MQ=255)

                                                       agggggggtttccGTTTTGCCTTTGGCCGCCCATAAACGGCGTTAACAGAGTGCGCAGCTCCCCG‑GGGGCCAGTGGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAAc                                                                                                                                                                                <  2:1213174/227‑1 (MQ=255)

                                                        cGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑TGGGCCAGTTGCGCCAGCTCAAGCACACCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGGGCCCAGAAAGCGA‑AATTGTCAGCCCTTT‑CCG‑GGACCGAACGTGGCCTGCCCCCCCAGCCATCCCGCATCTCGTGCCTgtggccaaa                                                                                                                                                                               >  1:1032665/1‑231 (MQ=255)

                                                        cGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCacc                                                                                                                                                                                >  2:1197339/1‑236 (MQ=255)

                                                        cGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACa                                                                                                                                                                               >  2:950816/1‑239 (MQ=255)

                                                              tgtATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTTTCCGCGCTTT‑CCG‑CGTCCTGACTTCGCCTGCCACTCCAGCAATCCCCCCTCCGGGGCCTGACGCAACAggcgac                                                                                                                                                                         >  2:222484/1‑234 (MQ=255)

                                                                  tCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCCGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAACCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTCTGCCCGCCGCacaagctacgtaac                                                                                                                                                                     >  2:968359/1‑226 (MQ=255)

                                                                        tGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACCGCGTACGAACATGGCg                                                                                                                                                               >  2:1155084/1‑239 (MQ=255)

                                                                             ttgcccccccttaagctgttgacaCAGTGTGCGCCG‑TCCCCTGGGGGCCAGTTGGCACAGCCCCATCACATGTTTCTGTGTCCCGGTTTCCGGT‑GCCGGTTCCTACATCGCTTGGCGTAGCTATTCCGGCGGAATCAGAAAGCGT‑ATTTGCCCGGTCTTC‑CCG‑GGTCTGGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGc                                                                                                                                                         <  1:849727/216‑1 (MQ=255)

                                                                             ttGTCCGCCCATAAACGGTTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGc                                                                                                                                                         <  2:1214457/239‑1 (MQ=255)

                                                                                               cTGTAACAGAGTGCGCAGCTCACCT‑GTGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGGCTGCGCCTGCCACTCCAGCCATCCCGcctcct                                                                                                                                                                                              >  1:945668/1‑185 (MQ=255)

                                                                                               cTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGcctcct                                                                                                                                                                                              <  2:945668/185‑1 (MQ=255)

                                                                                               cTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTCCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTACGCCCTTT‑CCG‑CGGCCTGACTGCGCCTGCCACTACAGCCATCCCGCCTCCTGTGCCTAGCGCAACACCGGACGAACATGGCTT‑TTCCCGCC‑AACACAGCTTTCg                                                                                                                                       >  1:1078798/1‑238 (MQ=255)

                                                                                                tgtacgaggtgccgtgtgtcccgt‑GGGGCCTGTTGCGCCAGCGCCGGCACCGTTCGTTTTTTCCCGGTTTCCAGT‑CCCTGTTCCAGTATCCGATGGCGACGGGTCTCCGGCGTGACAAGAAAGCTT‑ATTTGCCCCCGCTTC‑CCG‑CGTCCTGACGGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGc                                                                                                                                     <  1:241331/216‑1 (MQ=255)

                                                                                                 gTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGcc                                                                                                                                    >  2:341609/1‑239 (MQ=255)

                                                                                                        agTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGCCCAGTTccc                                                                                                                             >  2:271913/1‑237 (MQ=255)

                                                                                                        agTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGC‑AATTGTCCGCGCTCT‑CCG‑CGTACTGACTACGCCTGCCAATCCAGCCACACCGCCTCCTTCGCCTGAGGCAACAGCGCACGACCCTCGCCT‑TCGCGGAA‑AAAAACGCGTTCGGCCGGTTCgg                                                                                                                             >  1:178649/1‑238 (MQ=255)

                                                                                                         gTGCTCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGGTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGAGCGGAAAGCGG‑AATTGTCCGCCCTTT‑CCG‑CGTCCTGCCTGCGCCTGGCACTCCAGCCATCCCGCCTCCTCTGCCTTACGCAACAACGTACGACAAGTGCGTCTCTCT‑CC‑AAAACAGCCTTCGGCAAGCTc                                                                                                                               >  1:637317/1‑236 (MQ=255)

                                                                                                         gTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCACCAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTc                                                                                                                               >  2:396357/1‑236 (MQ=255)

                                                                                                         gTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTc                                                                                                                               >  2:269060/1‑236 (MQ=255)

                                                                                                         gTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCt                                                                                                                            >  2:476535/1‑239 (MQ=255)

                                                                                                         gTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGGTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCAATCCAGACATCCCGCCTCCTGTGCATACAGCACACGCGGCCGAAAATGACGT‑TGACTGCA‑AAAACAGCGGTCGGGCaaggccgt                                                                                                                            >  1:914468/1‑232 (MQ=255)

                                                                                                          tGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCtg                                                                                                                           >  2:1054925/1‑239 (MQ=255)

                                                                                                          tGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTCTGCCTGACGCAACAGCGTACGCACATGGCGT‑TCGCTGCA‑AACACAGCGTTCGGCCAGTTCGCtg                                                                                                                           >  2:401781/1‑239 (MQ=255)

                                                                                                          tGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCCCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCACCAGCGTCCGAACATGGCGT‑TCGCTGCA‑AAACAACCGTTGGGCCAGTTCCCtg                                                                                                                           >  1:949323/1‑239 (MQ=255)

                                                                                                            cgcAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCT‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCCCCTCCTGTGCCGACCCCAACAGCGTAGGAACATGGCGT‑TCGCCGCC‑AAAACACCGTTCGGCCAGTTCGCtgcc                                                                                                                         >  1:909732/1‑237 (MQ=255)

                                                                                                            cgcAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGCCTGCGCCTGCAACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGAACGAACATGGAGT‑TCGCCGCA‑AAAACCGGGTTCgccacgtgcgctagc                                                                                                                         >  1:1156793/1‑225 (MQ=255)

                                                                                                            cgcAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGCCGCAACCGCGCACGAACATGGCGT‑TCGCTGCA‑AAACCAGCGTTCGGCCAGTTCGCtcac                                                                                                                         >  2:498873/1‑239 (MQ=255)

                                                                                                            cgcAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCtgac                                                                                                                         >  2:890135/1‑239 (MQ=255)

                                                                                                            cgcAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCtgac                                                                                                                         >  2:77538/1‑239 (MQ=255)

                                                                                                            cgcAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCCCtgac                                                                                                                         >  2:1241944/1‑239 (MQ=255)

                                                                                                            cgcAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACACCGTTCGGCCAGTTCGCtgac                                                                                                                         >  2:972089/1‑239 (MQ=255)

                                                                                                            cgcAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCCCGCTGT‑CCG‑CGTCCTGACTGCGCCCGCCACTCCACCCCTCCCGCCTCCTGGTCCTGCCGCAACAGCGTACGAAAAAGGCG‑‑TCGCCGCACAAAAAAGCGTTCGCCAAATTCggggcg                                                                                                                         >  1:1087556/1‑234 (MQ=255)

                                                                                                                               aGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATCGCGT‑TCGCTGCA‑AAAACAGAGTTCGGCCAGTTCGCTGCCGGTGACGGTTTGCGGCtt                                                                                                       >  2:678802/1‑239 (MQ=255)

                                                                                                                                    cgcCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTg                                                                                                                                                                                                                     <  2:462170/126‑1 (MQ=255)

                                                                                                                                    cgcCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTg                                                                                                                                                                                                                     >  1:462170/1‑126 (MQ=255)

                                                                                                                                      ccAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACTAACATGGCAT‑TCCCTGCC‑ACAAAAGCCTTCAGCAGGTTCACATACGGGGAGGGtttcgacctttccgcgg                                                                                                >  1:158235/1‑225 (MQ=255)

                                                                                                                                       cgctcccgacctctCTTTCTGCTTCTCGTTTTCCAGT‑GCCTTCTCCACCTTCCCATGGCGTGGCGATCCCGGCGTCACCGAAATGGGT‑AGTTGTCCG‑GCTTTCCCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCcggcgg                                                                                               <  1:341609/226‑1 (MQ=255)

                                                                                                                                              aGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGGGGAAGGtt                                                                                         >  2:843280/1‑238 (MQ=255)

                                                                                                                                                 ccaTTTTGCTGTTTTCCGGTTTCCAGG‑GCCGTTTCCATCACCGATTTGCGTGGCTATTCCGGCGTGACCAAAAAGGGT‑AATTGTCCGCGCTTT‑CCG‑GGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCca                                                                                     <  2:949323/238‑1 (MQ=255)

                                                                                                                                                    tCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGACCATGGCGC‑TCGCTGCA‑AAAACAGCGTTCGGCCAGATGGCTGCCGGTGACGGTTGGCTGCTTTCCGGCGGAAGGTACCCACAg                                                                                  >  1:1279724/1‑239 (MQ=255)

                                                                                                                                                    tCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGAATTGCGTAGCGCTTCAGGCGTGACCAGATAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGCCCTGACTGCGCCGGCCACTCCAGCCGTCCCGCCTCCTCTGCCTGACGCAACAGCGGACGACCATGGCGT‑TGGCTGCA‑CAACCAGCGGTCGGCCAGTTGCCTGACGACGACCGGTTCCGCCCTACCCGCGGAAgcgccgcacag                                                                                  >  1:135105/1‑229 (MQ=255)

                                                                                                                                                     cTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGa                                                                                                                                                                       >  2:510271/1‑155 (MQ=255)

                                                                                                                                                     cTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGCTTGCGGCTTTCCGGCGGAAGGTTGCCACAg                                                                                  >  2:135608/1‑238 (MQ=255)

                                                                                                                                                     cTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACACCGTCCGAACATGGCGT‑TCGCTGCA‑CAACCAGCGTTCGGCCAGTTCGCTGACGGTGACGTTTGGCGGCTTTCCGCCGAAAGGTGGcccacaa                                                                                 >  1:298776/1‑234 (MQ=255)

                                                                                                                                                              ttCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCGCAGACGTTGAt                                                                          >  2:67724/1‑237 (MQ=255)

                                                                                                                                                              ttCCCGGTTCCAGTGGCCTTTTCCATCCCCGATTTGCGTGGCGATTCCGGGGGGACCGAAAAGCGT‑AATTCTCCCGCCTTC‑CCG‑CGTCCTGACGGCCCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATa                                                                         <  2:174459/239‑1 (MQ=255)

                                                                                                                                                               tCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTCCGGCCCGTTCGCTGACGGGTACGGTTTGCGGCTTTCCGGCGGAAGGTTGcaaacaccgttgataat                                                                       >  1:418433/1‑223 (MQ=255)

                                                                                                                                                               tCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCATGTGCCCGTCACAACCGCGCCCGAACCTGACGT‑ACCTCGCA‑CAAACAGCGTTCGGCCAGTTCGCTGACGGCAAAGGTTTTCGGTTTTCCGGCGGAAGGTTTCCACAAAGGTCGATAcg                                                                       >  1:1258369/1‑238 (MQ=255)

                                                                                                                                                               gatgtgtataagagaacggTTCCATCCTCGCATTGCGTAGCCGTTCCGGCGGTACCAAAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATa                                                                         <  2:641761/220‑1 (MQ=255)

                                                                                                                                                                         aGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGCAGGTTGCCACAGACGTTGATACTGGTTAAGACg                                                             >  2:424057/1‑239 (MQ=255)

                                                                                                                                                                                    ccatcccccctttGCGAGGCGATCCCGGCGTGCCCAGAAAGCTT‑AATTGCCCG‑GCTTTCCCG‑GGTCCGGATCGCCCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAAt                                                   <  2:1092519/228‑1 (MQ=255)

                                                                                                                                                                                    ccATCATCGCTTTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑ATTTGCCCCGGCTTT‑CCG‑GGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTAAGCAAt                                                   <  2:909732/239‑1 (MQ=255)

                                                                                                                                                                                     catccgcGCATTGCGTGGCGTTTCCGGGTGGACCAGAAAGCTT‑AATTGTCCGCCTTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATc                                                  <  2:1263421/233‑1 (MQ=255)

                                                                                                                                                                                      atcatgCCATGGCGTAGCGATCCCGGCGTGACCAGAAAGCGT‑AATTGCCCGCGCTTC‑CCG‑GGTCCTTACTGCCCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCg                                                 <  2:537137/239‑1 (MQ=255)

                                                                                                                                                                                      atcatcGAATTGCTTAGCTATTCCGGCGGGACCAGAACGCTT‑AATTGCCCGCGTTTC‑CCG‑CGCCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCg                                                 <  2:898310/239‑1 (MQ=255)

                                                                                                                                                                                            gCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGACCATGCCGT‑TCGCTGCA‑AAACCAGCGTTCGGCCAGTTCGCTGACGGTGCCGGTTTGCGGCTTTCCGCCGGAGGGTGGCCACAGACGTTGGTACTGGTAAAGACGGttcctttatacaacctg                                           >  1:641761/1‑224 (MQ=255)

                                                                                                                                                                                            gCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCAtata                                           >  2:34703/1‑239 (MQ=255)

                                                                                                                                                                                             cATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGTTACTGGTTAAGAACGTTGAGCAAtacagtaaaa                                          >  1:267662/1‑230 (MQ=255)

                                                                                                                                                                                                tgcgtgcggATTCCGGCGTGACCAGAAAGCTT‑AATTGCCCGCGCTTT‑CCC‑GGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAAcc                                       <  2:909677/231‑1 (MQ=255)

                                                                                                                                                                                                tgcgcacagttccgggCGTACCCAGAACGTGT‑TTTTGCCCCCGCTTC‑CGG‑ACTCGTCACGGCCCCTGCCACTCCAGCCCTGCCGCCTCCTGTGCCGGTCGCAACAGCGTTCGAACGGGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAAcc                                       <  1:641407/225‑1 (MQ=255)

                                                                                                                                                                                                  cGTAGCGATTCCGGCGTGCCCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCg                                                                                                                                                                                                    <  2:623000/82‑1 (MQ=255)

                                                                                                                                                                                                  cGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCg                                                                                                                                                                                                    >  1:623000/1‑82 (MQ=255)

                                                                                                                                                                                                  cGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACCGCGTTCGGCCAGTTCGCTGACGGGTACGGTTTGCGGCTTTCCGGCGGAGGGTTGCCCAAACCGTTGGTACTGGTTAGAGCGGTTGGGAAATCCCAATAAAACCCg                                     >  1:935776/1‑239 (MQ=255)

                                                                                                                                                                                                     agcgttccgggCGTGCCCAGAAA‑CGTAAATTGGCCGCGCTTT‑CCC‑GGTTCTGACTGCCCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGaa                                  <  2:135105/230‑1 (MQ=255)

                                                                                                                                                                                                      gCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAAc                                 >  2:331805/1‑239 (MQ=255)

                                                                                                                                                                                                        gATTCCGGCGTGACCAAAAAGCGT‑AATTTCCCGCGCTTC‑CCG‑CGTCCTGATTGCCCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACaa                               <  2:914468/239‑1 (MQ=255)

                                                                                                                                                                                                            ccGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAACCAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCCCAGACGTTGATACTGGTTAAGACGCGTGCGCAACCGCCAAAAAACACGGacaaaaata                           >  1:155248/1‑231 (MQ=255)

                                                                                                                                                                                                               gCGGGACCAAAGAGCGT‑AATTTGCCGCGCTTC‑CCT‑CGTCCTGACTGCTCCCGCCTCCCCAGCCTTCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATattatt                        <  2:1032665/239‑1 (MQ=255)

                                                                                                                                                                                                                   gACCAGAAAGCGT‑ATTGGCCCGGCCTTG‑CCG‑GGTCCTGACTGGGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTaa                     <  2:1258369/238‑1 (MQ=255)

                                                                                                                                                                                                                    aCCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGAGCCTGCCACTCCAGCCATCACGCCTCCTGTGCCAGACGCAACACCGTACGAACATGGCGT‑TCGCTGAA‑AAAAGCGCGTTCGGCCAGTTCGCTGTAGGTGAAGGTTTGCGGCTTTCCGGCGGGCGGTTGCCACCGACTTTGGTCCTGGTCAGAAGGGTTGAGCAATCGACAATCAACCCCGAAAAAGATTCttttaaa                   >  1:992218/1‑235 (MQ=255)

                                                                                                                                                                                                                     ccAAAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑GGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTa                  <  2:418433/239‑1 (MQ=255)

                                                                                                                                                                                                                       aGAAAGCGG‑AATGGTCCGCTTTTT‑CCG‑CGTCATGCCTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTAtt                <  2:1078798/239‑1 (MQ=255)

                                                                                                                                                                                                                        aaaaggtt‑AAGTGCCCCCGCTTC‑CCG‑GGCCCAGACTGCCCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTc               <  2:655867/232‑1 (MQ=255)

                                                                                                                                                                                                                        aaaaGCTT‑AATTGCCCCCCCTTC‑CCG‑CGCCCTGACGCCCCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACCGCGTACGAACATGGCGT‑GCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTc               <  2:992218/238‑1 (MQ=255)

                                                                                                                                                                                                                         aaagggttatttGCCCGCGTTTC‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTc               <  2:1279724/229‑1 (MQ=255)

                                                                                                                                                                                                                          acgCGT‑AATTCTCCGCGCTTC‑CCG‑GTCCCGGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTAtt                <  2:1105700/234‑1 (MQ=255)

                                                                                                                                                                                                                          aaGTGT‑AATTGCCCCCGTTGT‑CGG‑CGTCCTGACTGCCCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTc               <  2:920503/237‑1 (MQ=255)

                                                                                                                                                                                                                              tt‑AATGGCCCGCGCTTC‑CCG‑CTCCCTCGCGGCCCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTc               <  2:935466/232‑1 (MQ=255)

                                                                                                                                                                                                                              gT‑AATTGCCCGCGCTTT‑CCT‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCTCCTCCTGTGCCTGACGCAACAGGGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTAt          <  2:1219870/238‑1 (MQ=255)

                                                                                                                                                                                                                                   ttGCCCGCG‑TTT‑CCGCCGTCCGGCCGGCCCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACtt     <  2:155248/239‑1 (MQ=255)

                                                                                                                                                                                                                                    tGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAg                                                                                  >  2:293374/1‑161 (MQ=255)

                                                                                                                                                                                                                                      tCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCAGTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTACGACTCGTTAAAACGGTGTGGCAATGGCAATAAAACCCGGAACAATATTATTTaaacattaataattaattcac  >  1:38942/1‑220 (MQ=255)

CCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGGCTGTAACAGAGTGCGCAGCTCACCT‑GGGGCCAGTTGCGCCAGCTCCAGCACATCTTGCTGCTTTCCGGTTTCCAGT‑GCCTGTTCCATCATCGCATTGCGTAGCGATTCCGGCGTGACCAGAAAGCGT‑AATTGTCCGCGCTTT‑CCG‑CGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGT‑TCGCTGCA‑AAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCG  >  NC_000913/466891‑467356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A