Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F9 I0 R1 142 103.7 2603848 81.8% 2129947 227.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 861,951 T→G 13.1% H219P (CAC→CCC)  ybiW ← putative pyruvate formate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913861,9510TG13.1% 272.8 / 2.6 116H219P (CAC→CCC) ybiWputative pyruvate formate lyase
Reads supporting (aligned to +/- strand):  ref base T (46/50);  new base G (14/5);  total (61/55)
Fisher's exact test for biased strand distribution p-value = 4.69e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

TAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGT  >  NC_000913/861717‑862186
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           
tAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGtt                                                                                                                                                                                                                                          <  2:1100803/238‑1 (MQ=255)
 aGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCa                                                                                                                                                                                                                                        <  2:1155005/239‑1 (MQ=255)
           gagaTACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAAGGCAGCGAAACGGTCAATGTGTTCACTGACTTcc                                                                                                                                                                                                                               >  2:517694/1‑238 (MQ=255)
                 cTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCCCCAGCAGTTCATCGCGACGGCTTTCCCGGGTTTTGGG‑CGCGGCCATTTCACTCGCCAGGGCCGCGAAACGTTCAATGGGTTAACTGACTGccacccgac                                                                                                                                                                                                                        >  1:1116517/1‑235 (MQ=255)
                  tGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAACCGTTCAATGGGTTCACTGACTGCCACCAGccc                                                                                                                                                                                                                       >  2:204294/1‑237 (MQ=255)
                  tGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGGGTTCACTGACTGCCCCCCGCAc                                                                                                                                                                                                                       >  2:349140/1‑239 (MQ=255)
                    gTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑AGCGGCCATTTCACTCGCCAGGGCAGCGAAACGTTCAATGGGTTCCCTGACTGCCACCAGCACGa                                                                                                                                                                                                                     >  2:1086157/1‑239 (MQ=255)
                     tCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGat                                                                                                                                                                                                                    >  2:1226794/1‑239 (MQ=255)
                         tACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGGGTTCACTGACTGCCACCAGCACGATATCa                                                                                                                                                                                                                >  2:1124843/1‑239 (MQ=255)
                         tACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGAAGTTCATGGCGACGGCTTTCCCGGGTTTCGGT‑TGTGGCCATTTCACGCGACAGGGCAGCGAAACGTTCAATTGGTTCACTGCCCGCCCCCAGCACGATATCa                                                                                                                                                                                                                >  2:898967/1‑239 (MQ=255)
                          tccaCCAAACGGTACGGGTGGGCCGGTAGATTCGGTTGGCAAAATCAACTGGATGAAGTACCACAG‑GTGGAGCGGCT‑GCCAGAAAG‑TCTGGGGCGGCTGGTGGGCGGTAAGATCGCAGGTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGGCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCaa                                                                                                                                                                                                               <  1:931889/236‑1 (MQ=255)
                          aCGCCCAAAAGGTACTTGGTTGCCGTTAGGTTCGATGCGCAAAGTCAACTGGTAGAAGTAGCACAG‑TTGCAGCGCCT‑GACAGAAAG‑TCTGCGGCGGCTGGTTGGCGATAAGATCGGAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCAGTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCaa                                                                                                                                                                                                               <  1:369635/239‑1 (MQ=255)
                            gACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CCCGGCCATTTCACGCGCCAGGGGAGGCAAACGTTCAACTGGTTCACTGACTGCCACCAgacaggaatcaaac                                                                                                                                                                                                             >  1:623768/1‑226 (MQ=255)
                                 aaCGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCCGCGAAACGTTCCATGGGTTCACTGACTGCCACCAGCCCGATATCAATCGCtt                                                                                                                                                                                                         >  2:1200523/1‑238 (MQ=255)
                                 aaCGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCCCCAGCACGCTATCAATCCCtt                                                                                                                                                                                                         >  2:1164477/1‑238 (MQ=255)
                                 aaCGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCttt                                                                                                                                                                                                        <  2:281138/239‑1 (MQ=255)
                                 aaCGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGGGTTCACTGCCTGCCACCAGCACGCTATCAATCGCtt                                                                                                                                                                                                         >  2:1018341/1‑238 (MQ=255)
                                 aaCGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGAGTGGCGATAAGATCGCAGTTTTATGCCATCGCCAGCAGTTCATCGCGACGGCTTTCCAGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCACCGACAAGGTCACGGGGTTCACTGACTGCCCCCAGCCCgcaaacaacagcttt                                                                                                                                                                                                        >  1:1111084/1‑225 (MQ=255)
                                  aCGATACTGAGTGACCGTTAGATTCGAACTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCATCCCCT‑GCCAGAAAG‑GCTGCGGCGGCTGGTGGGCGATAAGATCGCAGGTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTc                                                                                                                                                                                                       <  2:940273/239‑1 (MQ=255)
                                         tGAGGGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGGAACCCAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCt                                                                                                                                                                                                          <  2:1263941/229‑1 (MQ=255)
                                           aGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCCCCAGCCCGATATCAAACGCTTTAAGGAAttc                                                                                                                                                                                               >  2:1183842/1‑237 (MQ=255)
                                           aGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTg                                                                                                                                                                                               >  2:477152/1‑238 (MQ=255)
                                           aGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGGTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCCGCCCGATATCAATCGCTTTCAGGAATTg                                                                                                                                                                                               >  2:189366/1‑238 (MQ=255)
                                            gTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGAAATCAATCGCTTTCAGGAATTGc                                                                                                                                                                                              >  2:706075/1‑238 (MQ=255)
                                              tgacgtttggttggtatcgCAAAATCCACTGGTGGAAGTACAACAG‑TTCCGGCCCCTGGCAAGAAGG‑TCT‑CGGGTGCTGTGGGGCGATAAGAATGTATTTTTTCTCCAGCCCACGCAGTTCTAAGCGACGGCTTTCGGGGGTTTCGGT‑TGCGGCCATTTCACGCTCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCAACAGCACGATATCAATCGCTTTCAGGAATTGCTc                                                                                                                                                                                            <  1:998828/220‑1 (MQ=255)
                                              gcccgttgaaatCGATCTTGAAAATCCACTGGATGAAGTAGCACCG‑TTGCAGCGCCG‑GCCAGAACG‑TCGGGGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCATCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCa                                                                                                                                                                                           <  1:861062/228‑1 (MQ=255)
                                              gACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCa                                                                                                                                                                                           <  2:147675/239‑1 (MQ=255)
                                              gACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCa                                                                                                                                                                                           <  2:201374/239‑1 (MQ=255)
                                                ccGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGAAGCGCAAAGTTCAATGAGTTAACTGACTGCCACCAGCACGATATCCATCGCTTTCAGGAATAGCTAAcc                                                                                                                                                                                         >  2:604095/1‑239 (MQ=255)
                                                ccGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGCTCATCGCGCCGGCTTTCGCGGGTTTCGGT‑CGCGGCCCTTTCCCGCGCCAGGGCCGCGAAACGTTCCATTTGTTCACTGCCTTCCACCACCACGACTTCAAACGCTTTCAGGAAtttccaccc                                                                                                                                                                                         >  1:49554/1‑232 (MQ=255)
                                                ccGGTAGATTAGATCTGCAAAATCAACTGGATGAAGTAACACGG‑GTGCAGCGCCT‑GCGAGAAAG‑TCTGCGGCGGCTGGTGGAGGATAAGAAAGCAGTTATCTGCCATCGCCAGCGAGTCATCGCGCCGGCTTTCGAGCGTTACGTT‑AGCGGCAATTTCACGCGCCAGGGCAGCGATGAGTGCCATGTTGTTACTGAGTGACACCCCGACGACATCAAACGCgttaccgaattcttcaac                                                                                                                                                                                         >  1:1282781/1‑224 (MQ=255)
                                                   ttAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGCCGGCTTTCGCGGGTTTCGGT‑CGCGGCCCTTTCACGCCCCAGGGGAGCGAAACGTTCCATGTGTTTCCTGGCTGCCCCCAGCCCGGCAACAAACGCATTCCGGAAttttggtccttt                                                                                                                                                                                      >  1:1263941/1‑229 (MQ=255)
                                                     aGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCCCTGCATGCCACCAGCCCGATATCAATCCCTTTCAGGAATTGCTCACCGTGTa                                                                                                                                                                                    >  2:630902/1‑239 (MQ=255)
                                                     aGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTa                                                                                                                                                                                    >  2:202083/1‑239 (MQ=255)
                                                        ttCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTaaa                                                                                                                                                                                  <  2:1111084/238‑1 (MQ=255)
                                                        ttCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTaaa                                                                                                                                                                                  <  2:1116517/238‑1 (MQ=255)
                                                        ttCGAGCTGAAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTaaa                                                                                                                                                                                  <  2:34665/238‑1 (MQ=255)
                                                           gATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCCGTTCATCGCGACGGCTTTCGCGGGTTTCGGA‑CGCGGCCCTTTCCCGCGCCAGGGCAGTGAAACGTTCAATGTGTTCACGGACTCCCACCAGCACCACATCAAACGCCTTCAGGCATTGCCCACCGCGTAAAACt                                                                                                                                                                               >  1:294676/1‑238 (MQ=255)
                                                               ttcAAAATCAACTGGAGGAAGTAACCCAT‑TTGCAGCGCCT‑TCCAGAAAG‑GCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAg                                                                                                                                                                          <  1:477152/237‑1 (MQ=255)
                                                                gaaaaaTCAACTGGAAAAAGGAACCCAG‑TTGCAGCGCCG‑TCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGc                                                                                                                                                                         <  2:408630/237‑1 (MQ=255)
                                                                gCAAAATCAACGGGATGAAGTAAAACTG‑TTGCAGCGCCT‑GCCAGAATG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTGTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGc                                                                                                                                                                         <  1:66179/239‑1 (MQ=255)
                                                                agatgtgtagaagaggaaggTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAg                                                                                                                                                                          <  2:964920/219‑1 (MQ=255)
                                                                 cAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGc                                                                                                                                                                         >  2:1154104/1‑238 (MQ=255)
                                                                 cAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCACCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGCTATCAATCGCTTTCAGGAATTGCTCCCCGGTTAAATCTTCCAga                                                                                                                                                                         >  2:1037231/1‑237 (MQ=255)
                                                                 cAAAATCAACGGGAGGAAGTAAC‑CAGTTTTCAGCGCCC‑TCCAAGAAG‑TCTGGGGCGGTTGGGGGGGGAAAAGATCGCAGTGTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCa                                                                                                                                                                        <  1:1154104/239‑1 (MQ=255)
                                                                         aCTGGATGAAGTAACACAG‑TTGCAGCGCCG‑GCCAGAAAG‑GCGGCGGCGGCTGGGGGGCGATAAGATCGCAGGTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAg                                                                                                                                                                          <  2:602603/229‑1 (MQ=255)
                                                                                   gTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGAGGGCGATCAGATCGCCGTTTTCTGCCATCGCCAGCCGGTACTCGCGACGGCTTTCGCGGGTTTCGTT‑CGCGGCCCTTTCACCCGCCAGGGCAGCGAAACGGTCACTAGGTTCCCTGCCTGCCCCCCGCCCGCTCTAAATCCCTTTTCCGACTTTCTCACCCTGGAAATACTCCAGCCCCCTCaagccttgcgttt                                                                                                                                                      >  1:602603/1‑227 (MQ=255)
                                                                                       cacaG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGGGATAAGAACGCAAGTTTCTGCCATCGCCAGCAGTTAATCGGGACGGCTTTCGCGGGCTTCGGT‑CCCGGCCATTTCACACGCCAGGGGAGCGGAACGTTTCATGTGTTAAGCGCCATCCACCACCCACATAACAACAGCGTGCAGGAATTGCACCCCCTGGAAACGTTCCAacacataccgtgtctgggcgacga                                                                                                                                                   >  1:745307/1‑218 (MQ=255)
                                                                                             ttGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGGGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGt                                                                                                                                              >  2:211569/1‑238 (MQ=255)
                                                                                              tgcgtccccT‑GCCAGAAAG‑TCTGCGGCGGCTGGGGGGCGATAAGGCCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTc                                                                                                                                            <  2:523385/232‑1 (MQ=255)
                                                                                              tGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTTGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTc                                                                                                                                            <  2:407450/239‑1 (MQ=255)
                                                                                              tGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGtt                                                                                                                                             <  2:294676/238‑1 (MQ=255)
                                                                                                   cgcCT‑GCCAGAAAGTTCTG‑GGCGGCTTGTTGGCGATTAGAGCGGAGTTTTCCGCCCACCCCAGCCGGTCCTCGCGACGGCTTTCGCTGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTa                                                                                                                                       <  1:686742/239‑1 (MQ=255)
                                                                                                   cgcCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGCCGTTCCGCt                                                                                                                                        >  2:269079/1‑238 (MQ=255)
                                                                                                     gctggCCAGAAAG‑TCGTCGGGTGCGGGGGGGCGATAAGATCCCAGTTTCCTGCCAGCGCACGCAGTGCATCGCGACGGGGTTCGCGGGTTTCGGT‑CGCGGCCATTTCCCGCGCCAGGGCAGCGAAACGTTCAATGTGTTTACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGCTGCGCGAGCGACGTTCCGCtc                                                                                                                                       <  1:1164477/234‑2 (MQ=255)
                                                                                                     ccT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCACTCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAg                                                                                                                                                                          >  1:221715/1‑202 (MQ=255)
                                                                                                     ccT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAg                                                                                                                                                                          <  2:221715/202‑1 (MQ=255)
                                                                                                     ccT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGATTTCTGCCATCCCCAGCAGTTCATCGCCACGGCTTTCCAGGGTTTCGGT‑CGCGGCCATTTACCGCGCCACGGCAACCAAACGTTCAATGGGTTCACTCACGCCCAACCGCCCGATTCCAATCCCTTTCAGGACTTCATCACACTGTAAATCTTTCCCCACCGTCCGGTTGactggccacgcgcggtccccccag                                                                                                                                     >  1:830303/1‑216 (MQ=255)
                                                                                                       t‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCCCGAGCGACGTTCCGCTACtt                                                                                                                                    >  2:510487/1‑238 (MQ=255)
                                                                                                         gCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGAGGCGCGAGCGACGTTCCGCTACTTc                                                                                                                                   >  2:474228/1‑238 (MQ=255)
                                                                                                                aG‑TCTGCGGAGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCTGCAATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCa                                                                                                                           <  2:745307/239‑1 (MQ=255)
                                                                                                                 agctgggggggggtgggggGCGATAAGGCCGAAGTTTTCTGCCCGCGCCAGCAGTTCATCGCGCCGGCTTTCGCGGGTTCCGGG‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCa                                                                                                                           <  1:706075/226‑1 (MQ=255)
                                                                                                                    tctcggcggTTGGTGGGGCATTAGGTCCGCGTTTTTGGCCCTCGCCATCAGTTCATCGCGCCGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAg                                                                                                                          <  1:202083/234‑1 (MQ=255)
                                                                                                                     tGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCCCCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTCCTTCCTCGCGCAGAcc                                                                                                                       >  2:326530/1‑239 (MQ=255)
                                                                                                                     tGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCCCAGGcc                                                                                                                       >  2:1298325/1‑239 (MQ=255)
                                                                                                                      gcggcgGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGGTTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGAcc                                                                                                                       >  2:143320/1‑238 (MQ=255)
                                                                                                                        ggggggtgtggggTGATAAGGTCGGAGTTTTCCGCCGACCCCAGCAGGTGCTCGCGAAGGGTTTCGCGGGTTGCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAAGCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATc                                                                                                                    <  1:1183842/229‑1 (MQ=255)
                                                                                                                         gcggcTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCgcgag                                                                                                                                                    >  1:201096/1‑206 (MQ=255)
                                                                                                                           gggtggtggGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCaa                                                                                                                  <  2:784382/235‑1 (MQ=255)
                                                                                                                                          agtacGCACTTTTTTGCCATGGGCCGGAGTTCGTCGCGACCGCTTTCGCGGGTTTCGGT‑CGCCGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCcagcag                                                                                                  <  2:938312/235‑1 (MQ=255)
                                                                                                                                             tcgtcgTTTGCTGCCTGCGCCAGCAGTTGATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCcagcagca                                                                                                <  2:1013191/233‑1 (MQ=255)
                                                                                                                                              cGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCCGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGCCAGGTTGATGCGCGAGCGACATTCCGCAACTTCCCTCCGCAAGCCACCAAACCCTTTTTccaccacccgc                                                                                              >  1:1004471/1‑234 (MQ=255)
                                                                                                                                              cGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGTGATTGCTCACCGTGTAAATCTTCCAGCACCCTCAGGTTGATGCGGGAGGGACGTTCCGCTACTTCCTCGCCCAGGCCACACAGCCCCTTTTCcagcagcagg                                                                                              >  1:498678/1‑238 (MQ=255)
                                                                                                                                                cAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCgg                                                                                            >  2:691004/1‑239 (MQ=255)
                                                                                                                                                   ttttCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCAAGCAGCAGCGGGaa                                                                                         >  2:394863/1‑239 (MQ=255)
                                                                                                                                                     ttCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAAt                                                                                       >  2:959774/1‑239 (MQ=255)
                                                                                                                                                      tCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCACCGGGAAAt                                                                                       >  2:1026608/1‑238 (MQ=255)
                                                                                                                                                             tCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGcc                                                                               >  2:440011/1‑239 (MQ=255)
                                                                                                                                                             tCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGGGTAAATCTTCCAGCACCGTCATGTTGATGCGCGAGCGACGTTCCGCTACTTCCCCGCGCAGACAACCAAGCCCTTTTTCCCGCGACCGCGGGAAATTTCCCGcc                                                                               >  1:1011349/1‑239 (MQ=255)
                                                                                                                                                                     ggTTCCTCGGGGCGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATTGGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTg                                                                          <  2:794187/235‑1 (MQ=255)
                                                                                                                                                                             gcgACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCgg                                                               <  2:49554/239‑1 (MQ=255)
                                                                                                                                                                              cgctggCTTTCCGGGGTTTCGGT‑CCCGGCAATTCCCCGGCCCAGGGCAGCGAAACGTTCAATGGGTTAACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCGGCCCCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGa                                                              <  1:1226794/235‑1 (MQ=255)
                                                                                                                                                                               ggggtttttCGGGGGTTTCGGT‑GGCGGCATTTTCCCGCGCCAGGGCAGCTAAACGTTCAATGTGTTCACGGACGGCCACCAGCACGAGATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTGCCGCTACTTCCCCGCGCAGACCATCAAGCCCTGTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAg                                                             <  1:1018341/233‑1 (MQ=255)
                                                                                                                                                                                  ggCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGGGCGCATCGCCCGAggcc                                                          >  2:113735/1‑237 (MQ=255)
                                                                                                                                                                                     ctcgccgtgTTTGGGT‑CGCGGCCATTTCCACCGCCCTGGCATCTAAACGTTAAATGTGTTCACTGACTTCCACCAGCACCATTCCAATCGCATTAAAGAATTGCTACCCGTGGAAATCTTCCAGCCCCATCAGGTTGATGCCCGATCGACGGTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCata                                                       <  1:366282/231‑1 (MQ=255)
                                                                                                                                                                                      ttCGGGGGTTTCGGT‑CGCGGCCATTTCCCGCGCCAGGGCAGCGAAACGTTCAATGTTTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCata                                                       <  2:129052/238‑1 (MQ=255)
                                                                                                                                                                                        ctcgGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTc                                                                                                                                                                   <  1:1029267/126‑1 (MQ=255)
                                                                                                                                                                                        cgcgGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTc                                                                                                                                                                   >  2:1029267/1‑128 (MQ=255)
                                                                                                                                                                                             ttttGGGT‑CGGGGTCTTTTCCCGCGCCGGGGCGGGGAAACGTTTAATGTTGTCACTGACTGCCACCAGCACTATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTGAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCtt                                                <  1:440011/237‑1 (MQ=255)
                                                                                                                                                                                             gTTTTGGTCCGCGCCCATTTCACGCGCCAGGGCAAGGAAACGTTCAATTGTTTCCCTGGCGGCCCCCAGCCCGATATCAATCGCTTTCAGGAATTGCTCACCGTTTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCtt                                                <  1:1200523/239‑1 (MQ=255)
                                                                                                                                                                                             gTTTTGGT‑CGCGGACATTCCCCGCGCCAGGGAAGGGAAACGTTAAATGTGTTTCCTGACTGCCCCCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGGGTAAATCTTCCAGCACCGGCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCtt                                                <  1:349140/238‑1 (MQ=255)
                                                                                                                                                                                             gTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCtt                                                <  2:287847/238‑1 (MQ=255)
                                                                                                                                                                                                        cgGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATg                                     >  2:747645/1‑239 (MQ=255)
                                                                                                                                                                                                        cgGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGCCAAATTGCCTTCCGCTTTAATg                                     >  2:416924/1‑239 (MQ=255)
                                                                                                                                                                                                            cttttCCCGCCCCGGGGCCGCAAAACTTTCAATGTGTTCACTGCATCCCCCCAGCCCGATACACACCGTTTTCAGGAATTCGTCCCCGTGTAATTCTTCCAGCACCGTCAGGTTGATGCGCGGGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTc                                 <  1:959774/237‑1 (MQ=255)
                                                                                                                                                                                                                           ggCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCACCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGCCCTATTTCCTTCCCCTTTAATTATTCCggcgcccacaaga                   >  1:1174567/1‑227 (MQ=255)
                                                                                                                                                                                                                            gCAGGGAATCGTTCAAGGGGTTCACTGACTGCCACCAGCACGATTTCAATCGCTTTCAGGAATTGCTCTCCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCcagcag                    <  2:108526/236‑1 (MQ=255)
                                                                                                                                                                                                                                cGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCCCCGCCAGGGGCGCCTCGCCGGAGGCCATATTGCCTTCCGCTTTAATGATTCCGGCTGCCAGCAGAACtttt             >  1:1060023/1‑239 (MQ=255)
                                                                                                                                                                                                                                cGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCCGCAGACCtttt             >  2:1031796/1‑239 (MQ=255)
                                                                                                                                                                                                                                           aTGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCATCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCCCCGGAGGTCAAATTGCATTCCGCTTTAAGGATTCCGGGCGCCAGCCGACCTTTTTGCTAATCTGt  >  1:251166/1‑239 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           
TAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAG‑TTGCAGCGCCT‑GCCAGAAAG‑TCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTTCGCGGGTTTCGGT‑CGCGGCCATTTCACGCGCCAGGGCAGCGAAACGTTCAATGTGTTCACTGACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGT  >  NC_000913/861717‑862186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A