Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F9 I0 R1 142 103.7 2603848 81.8% 2129947 227.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,065,745 A→G 19.3% E54G (GAG→GGG)  agp → glucose‑1‑phosphatase/inositol phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,065,7450AG19.3% 117.5 / 3.6 57E54G (GAG→GGG) agpglucose‑1‑phosphatase/inositol phosphatase
Reads supporting (aligned to +/- strand):  ref base A (16/29);  new base G (10/2);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 7.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTGCGAACCTGTTCCCGGAAAAAAGTCATATTTCTGTCACACTCTTTAGTGATTGATAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACA‑CCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGA  >  NC_000913/1065507‑1065982
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                
tttgGCGAACTTGTTCCCGGAAAAAAGTCATTTTTCTGTCACACTCTTTAGGGATTGATAACAAAAGAGGTGCCAGGAATGAAAAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGa                                                                                                                                                                                                                                                  <  2:209637/239‑1 (MQ=255)
                    aaaaaaGTCATATTTCTGTCACACTCTTTAGTGATTGATAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAATGCTCATGATGAGCCGCCATAACTTACGGGCGCCGCT‑GGGGAACAATGGCAGGGGGCTGGGGCAGTCGACGCCGAATAAAt                                                                                                                                                                                                                              >  2:291799/1‑239 (MQ=255)
                    aaaaaaGTAATTTTTCTTCCACCCTTTTTAGTGATTGAAAACAAAAGAGCTGCCAGAAATGAACAAACCGCTAATCGCCGCAGCTGGGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAAt                                                                                                                                                                                                                              <  2:1176675/239‑1 (MQ=255)
                       aagaaactttttCTGCCACCCTTTTTAATGATTGACAAAAAAAGGGGTGACAAGAATGAAAAAAACCCTAACCGCCGCAGGTGGGGTAGGGATAGTTTATCTCGCTTCAAACGCTCAGG‑AACAAACCGTACCGGAAGGCTATCAGCGGCA‑GCAAGGGCTCAGGGTGGGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGc                                                                                                                                                                                                                           <  1:1011829/230‑1 (MQ=255)
                       aaaGTCATATTTCTGTCACCCTCTTTAGTGATTGAAAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGGGGCAGGGATAGTTTTACTCGCTTAAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGc                                                                                                                                                                                                                           <  2:889639/239‑1 (MQ=255)
                                           ctctTTAGTGATTGATAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTAATCGCTTCAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTAAA‑GCAAATGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGGGTGCTGGGGGAGTCGACGCCGAATACATGGCCAGAATGGGAcctgtccctt                                                                                                                                                                                                       >  2:1011829/1‑230 (MQ=255)
                                              tttAGTGATTGATAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCCTCAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAACTGCTCATGATGAGCCCCCATAACTTACGCGCGCCGCG‑TGCGCACATATGCAGGGTGCTGGGGCAGTCTACGCCGACAAGAAGGCCAAAATagggccgcccgcggggg                                                                                                                                                                                                    >  1:700121/1‑225 (MQ=255)
                                                    gattgaaAACAAAAGAGGTGCAAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACt                                                                                                                                                                                               <  2:700121/238‑1 (MQ=255)
                                                     attgatAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGG‑CACAAACCGTACCGGAAAGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACCTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGGGCAGTCGACGCCGAAAAAATGGCCAGAATGGGACGTCCCCGAGGGGCACCTc                                                                                                                                                                                              >  2:115381/1‑238 (MQ=255)
                                                     attgatAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGGTTTACTCGCTTCAAACGCTCAGG‑CACAAACCGTACCGGGAGGCTATCAGCCACA‑GCAAGAGCTCACTAAGAGCCGCCCTAACTTACGGGCGCCGCTCGGCGACCACGTGC‑GGGGGCTGGAGCCAGCGCAGCCGAATAAATAGCCCAAACGGGAGCGCCCCGGGGGGCagcacc                                                                                                                                                                                             >  1:25075/1‑234 (MQ=255)
                                                      ttttaaaaaaaaGGGGGGCAGGGGATGAACAAAATGATAAGCGCCGCGGTGGTGGGGGGGATTGTTTATCCCGCTTCAAGCGCTCAGG‑CAAAATCCGTACCGGAAGGCTATCAGCGAAA‑ACAGGTGCTAATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGTGACGTCCCCGGTGGGCAACTca                                                                                                                                                                                             <  1:1118629/230‑1 (MQ=255)
                                                        gatAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCCTAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGGTTGCTGGGGCAGTCGACGCCGAATAAATGGCCCGAATGGGACGTCCCCCGTGGGCAAATcacc                                                                                                                                                                                           >  2:286777/1‑238 (MQ=255)
                                                        gatAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGGGCAGTCGACGCCGAATAAATGGCCCGAATGGACCGCCCCCGCGGGCCAACTaacac                                                                                                                                                                                          >  2:607740/1‑237 (MQ=255)
                                                        gatAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACCCTCAGG‑CACAAACCGTACCGGAAGGCTATCACCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACTTCCCCGGTGGGCAAccactc                                                                                                                                                                                           >  2:1013092/1‑233 (MQ=255)
                                                         aaaaaaaaaGGGGTGCCAAGAAAAAAAAACACCTAAACCGCCGCAGGTGGGGCGGGGATATTTTTACTCGTTTCAATCG‑ACAGGCCACAACCCGTACGGGAAGGCTACCAGCTACA‑GCAAGTGCTAAGGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTcaccac                                                                                                                                                                                         <  1:1040850/234‑1 (MQ=255)
                                                          tAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCCTAACTTACGTGCGCCGCT‑GGCGAACAAGGGCAGTGGGCTGGGGAAGTCGACGCCGAAAAAATGGCCAGAATGGGACGGCCCCGGGGGGCAAACCcccacc                                                                                                                                                                                        >  2:1250065/1‑239 (MQ=255)
                                                                aaGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAAATCACCCCCCAAgg                                                                                                                                                                                   >  2:1118629/1‑238 (MQ=255)
                                                                aaGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGGGTGCTGGGGCAGCTGACGCCGAATAAATGGCCAGAATTGGAAGACCCCGGTGGGAAACTCCCACCCAAAgag                                                                                                                                                                                  >  2:159849/1‑237 (MQ=255)
                                                                aaGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTAATCGCTTCAAACGCTCAGG‑CCAAAAACGTACCGGAAGGCTATCAGCTACA‑GCAAATGCTCATGATGAGCCGCCATAAATTACGTGGGCCCCT‑GGCGAACAATGGCAGTGGGGTGGAGCCATCGGACCCGCAAAAAAGGCCACAAAGGGACGGCCCCGGGGGGGAAACCAACACAAAAgag                                                                                                                                                                                  >  1:164953/1‑237 (MQ=255)
                                                                     gTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGGGAACAATGGAAGTGTGCTGGGGCAGTCGGCGCCGAATAAATGGCCGGAATGGGACGCACACGGGGGGGAACTCACCACCAAAGGTggggg                                                                                                                                                                             >  2:87484/1‑236 (MQ=255)
                                                                     gTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGG‑CACAAACCGTAACGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCCTAACTTACGTGCGCCGCT‑TGCGAACAATGGCAGTGTGCTGGGGCAGTCGACCCCGAATAAATGGCCCGAATGGGAGGTCCCCGGTGGGCAACTCACCACCAAAGGGGGCGt                                                                                                                                                                             >  2:131808/1‑239 (MQ=255)
                                                                          attaaTAAACAAACCGCTAGTCCCCCCGTGTGGGGCAGGGATTGTTTTGCTCGTTTCACACCCTCAGC‑GCCAAACTGTCACGGAAGGCTACCAGCTAAA‑GGAAGGGGTGAGGAGGGACCGCCATAACCTACGGGCGCCGCT‑GGCGAACAATGGCAGGGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATTGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCg                                                                                                                                                                        <  1:1083565/236‑1 (MQ=255)
                                                                            aaaaaaaCAAACCGCTAAAGCCCGCAGGTGTGGCAGGGATAGTTTTACTCCCTTAAACCGTCCAGG‑CAAAAACCGTCCCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGaa                                                                                                                                                                      <  2:562805/234‑1 (MQ=255)
                                                                             aaTGAACAAACCTCAAATCCCCGCAGCTGTGGCAGGGATAGTTTTCCCCCTTACAAACGCTCAGG‑CACAACCCGTACCGGAAGGCTATCAGCTACA‑GCAAGTCCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCg                                                                                                                                                                        <  2:213351/236‑1 (MQ=255)
                                                                             aaTGAAAAAACCGCAAAACGCCGAGGCGGGGGCGGGGATAGTTTTACTCGCTTCAAACGCCCAGG‑CACAAACCGAACCGGAAGGTTATCAGCTACA‑CGAAGTGCTCATGATGGGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCg                                                                                                                                                                        <  2:1127041/236‑1 (MQ=255)
                                                                             aaTAAAAAAACCCCTAATCGCCCGAGCTGGGGCGGGGATAGTTTTACTCCCTTCAAACCCTCAGG‑CCCAAACCGTACCGGAAGGGTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCg                                                                                                                                                                        <  2:586699/236‑1 (MQ=255)
                                                                             aaTAAAAAAAACGCTAACCGCCGCAGTGGGGGCAGGGATAGTTTTCCCCGCTTCAAACGCTCAGG‑CAAAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGaa                                                                                                                                                                      <  2:57223/238‑1 (MQ=255)
                                                                                   cAAACCGCAAATCGCCGCAGCTGGGGCAGGAATAGTTTTACTCGTTTCAACCGCTCAGC‑CACAAACCGTCCCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGtata                                                                                                                                                               <  2:164953/239‑1 (MQ=255)
                                                                                        cGCTAATGGCGGCAGGTGTGGCAGGGATAGTTTTCCTCGCTTCAAACGCTCAGG‑CAAAAACCGTACCGGAAGGCTATCAGCTACA‑GCAGGTGCTCATGAGGGGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGc                                                                                                                                                         <  2:535840/239‑1 (MQ=255)
                                                                                                               ggggTATTTTTACTCTCTTTAACCGCTGCGA‑CCCAAACCGTACGGCAAGCGAATCAGCCTGA‑GGAAGGTATAGTGTGGGGCCGCGATAACTTACGGGCGGCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAAtggctgg                                                                                                                                  <  1:443675/238‑1 (MQ=255)
                                                                                                                      ttttACCCGCTTAAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCa                                                                                                                                <  2:915196/233‑1 (MQ=255)
                                                                                                                           cTCGCTTCAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTCCCCGAAGTGTATA‑TGGGCCCTTACT‑TGCGTAAATGCCTGGCGCGGCAGGGGAt                                                                                                                     >  2:98570/1‑239 (MQ=255)
                                                                                                                                ttCAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGGGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGATCAGGGGTTGGGGa                                                                                                                >  2:1215320/1‑239 (MQ=255)
                                                                                                                                ttCAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGGGCCCCAAGGGGAAA‑TGGGCCATTTCA‑TGCGCGAATGGCGGGGAAGGCAggggggatgtg                                                                                                                >  1:915196/1‑232 (MQ=255)
                                                                                                                                          catg‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCA‑GGCGACCAATGGCAGTTTGCTGGAGCAGTCGACGCCGAATAAATGTCCCGAATGGGACGTCCCCGGTAGGCAACCCACCACCCAAGGTGGCGTGTTCGAAACGCCTA‑AGGGCCATTACAGTGAGT‑AATGGGTGGCCGAGCGggggtggggggaatgagggga                                                                                                      >  1:857814/4‑222 (MQ=255)
                                                                                                                                          cAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTTCCCGGTGGGCAACTCCCCACCAAAGGGGGCGTGCTCGACGGGAAAA‑TGGGCCATTCAA‑TGCGTGAATGGCGGGACGGGCAgggggggggtgaaccggggga                                                                                                      >  1:784920/1‑222 (MQ=255)
                                                                                                                                                ccaAACCGTCCGGGAAGGCTACAAGC‑ACAGGAAAGTGTTATGGAGGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGcc                                                                                                 <  2:25075/238‑1 (MQ=255)
                                                                                                                                                            ggAAGGCTGTCAGCTACA‑GCAAGTCCTCAGGATGACCCGCCATAACTTACGGGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTaca                                                                                     <  2:538338/239‑1 (MQ=255)
                                                                                                                                                            ggAAGGCTATCAGCTACA‑GCAGGGTATCATGGTGGGCCGCCATACCTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAATTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTaca                                                                                     <  2:205121/239‑1 (MQ=255)
                                                                                                                                                                    aTCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGGGAAATCGGGGGAATGCCCGCCGCCGTACA‑CCGTTtat                                                                            >  2:443775/1‑239 (MQ=255)
                                                                                                                                                                      cAGCTAAA‑GCAAGTGCTCATGATGGACCGCCAAAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGAGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGCGGGGCAACTCACCCCCAAAGGTGGCCGGCTCGAAGTG‑ATATTGGGCCATTCAA‑TGCGTGACTGGCGGTCAGAGCAGGGGAGGGGGAAACAGGTGAATTGCCGCCCGCCGAACC‑CCGttattg                                                                           >  1:1078507/1‑234 (MQ=255)
                                                                                                                                                                                    tGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGGGGGCAACTCACCACCAAAGGAGGCGAGCTCGAAGGGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAAGGGCCGCCGCCGCAAA‑CCTTTTGTGCCTATGACAAAAg                                                              >  1:729625/1‑238 (MQ=255)
                                                                                                                                                                                                     cATAACTTCCGTGGGGCGGT‑TGCATACAATGGCCTTGGTCGGGGGCACGCGACGTCGAATAAATGGCCAGAATGGGACGGCCCCGGTCGCCAACTCACCACCAAAGGGGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACA‑CCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGc                                            <  1:291799/239‑1 (MQ=255)
                                                                                                                                                                                                       aaacgtaccgtcgccccT‑GGCAAACAAAGGCAGTTGGCGCAAGCAGTTGTCCGCGAATAAATGGCCGAAATGTAACCTCCCCGGTGGGCACCTCAACACCAAAGGTGGCGTGTGTGAATTGTATT‑TGGTCCATTACA‑TGCGTGAATGGCTGTCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACA‑CCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGa                                          <  1:87484/228‑1 (MQ=255)
                                                                                                                                                                                                            tACGTTCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACA‑CCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGca                                     <  2:399610/239‑1 (MQ=255)
                                                                                                                                                                                                            tACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGGCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACA‑CCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGca                                     <  2:380825/239‑1 (MQ=255)
                                                                                                                                                                                                               gTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACC‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAACTGGGGGAATGCCCGCCGCCG‑ACACCCGTTTATGCCCTTGCCCAAAATCTGCACCGAACCGGTGCGACCGCCCAg                                  >  1:65068/1‑239 (MQ=255)
                                                                                                                                                                                                               gTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGAAGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACA‑CCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAg                                  <  2:420914/239‑1 (MQ=255)
                                                                                                                                                                                                                  cgccgcT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATCAATGGACAGAATGGGACGTCCCCGGTGGGCAACTAACCACCAAAGGATGCGTGCTCGAAGTGAATA‑TGGGCCATTACA‑TGCGGGCAGGGCTGGCAACGACGGGGATGGGCAAATCGGGGTAATGCCACCCGCCGTGCA‑CCGTTACTGCCCAGGAGAAAAACCTGCAACGTCCCGTTGAAACCgacaact                                 >  1:285113/1‑231 (MQ=255)
                                                                                                                                                                                                                            gtcaCAATGGCAGGTTGTTGGGTCAGTCGGCGCAGAAGAAATGCCCAGAATGGGAGGTCCCGGGGTGGCAACCAACATCCAAAGGGTGAGTGCGCGAAGTGTATG‑TGGGCCATTACA‑TGCGTGAAGGGCTGGCAGCGCAGGTGATGGTGAAAGCGGGGGAAGGCCCGCAGCCGTAGA‑CCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTttatgaa                       <  1:778868/235‑4 (MQ=255)
                                                                                                                                                                                                                               aCAATGGGAGTTTGCTGGAGCAGTCGGCCCCGAATAAATGGCCAGAATGGGACGGCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGATCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACA‑CCGTTTATGCCTATGCCGATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCgg                    <  2:310642/238‑1 (MQ=255)
                                                                                                                                                                                                                                cAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGGGCAGGGGATGGGGAAATCGGGGGAATGCCCGGCGCCGTACA‑CCGTTTATGCCTTTGCCCATAGTCTACAACGAACCGTTGCGACCGCCAAGGTCTTTAATAACcgcg                  >  1:619154/1‑239 (MQ=255)
                                                                                                                                                                                                                                cAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACA‑CCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGgcg                  <  2:423482/239‑1 (MQ=255)
                                                                                                                                                                                                                                 aaTGGCAGTGGGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGGCGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACA‑CCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGgcg                  <  2:921141/238‑1 (MQ=255)
                                                                                                                                                                                                                                        gggtgCTGGAGCAGTCGACGCCGAATAAAGGGCCAGAATGGGACGTCCCCGGTGGGCAACTACCCACCAAGGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACA‑CCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTccc           <  2:590132/236‑1 (MQ=255)
                                                                                                                                                                                                                                         tgtgCTGGAACAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACA‑CCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCg          <  2:689765/238‑1 (MQ=255)
                                                                                                                                                                                                                                         tgggcgggACCAGGCGACGCCGAATAAAGGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACA‑CCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCg          <  2:668293/232‑1 (MQ=255)
                                                                                                                                                                                                                                                gAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTAAA‑TGGCCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGGGAAACCGGGGGAATGCCCGCCGCCGTAGA‑CCGCTTTGGCCTATGCCAAAAGGCTGCAACGGACCGCTGCGCCCGCAAGGTTCTTTATTACCgggcacatcaccgggggggg  >  1:721419/1‑221 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                
TTTTGCGAACCTGTTCCCGGAAAAAAGTCATATTTCTGTCACACTCTTTAGTGATTGATAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGG‑CACAAACCGTACCGGAAGGCTATCAGCTACA‑GCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCT‑GGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATA‑TGGGCCATTACA‑TGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACA‑CCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGA  >  NC_000913/1065507‑1065982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A