Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R1
|
52 |
59.5 |
1410130 |
88.0% |
1240914 |
215.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
729,603 |
G→C |
13.4% |
A7A (GCG→GCC) |
rhsC → |
Rhs protein with putative toxin domain, putative neighboring cell growth inhibitor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 729,603 | 0 | G | C | 13.4%
| 46.0
/ 2.1
| 26 | A7A (GCG→GCC) | rhsC | Rhs protein with putative toxin domain, putative neighboring cell growth inhibitor |
| Reads supporting (aligned to +/- strand): ref base G (10/12); new base C (2/2); total (12/14) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 |
CAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGC > NC_000913/729399‑729810
|
cAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGCAAACCGGCAGCCCGCCAAGGAGAAAAAACGCAGt > 1:41792/1‑227 (MQ=25)
tacgatgAATATTTAAAAATCTTTTTACAAATCGAAATATTAATGCAGGAAATATATATAAATATTTTCCTAATGGTTCTTAGCTGACAGATATCACACATAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCGGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCa < 1:328880/222‑1 (MQ=18)
tCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGGAGCATTTTCCCGGG‑GTTACCCgg > 1:61128/1‑228 (MQ=255)
cAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGAAGCCTTGTCCACGG‑TTCATCCGGGGGACGCa > 2:483491/1‑227 (MQ=255)
aTCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGAATGGCGGGAACATTGTCCCGGGTTTCGGCCggggaagccac > 1:74072/1‑220 (MQ=18)
tatatTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCa < 2:61128/228‑1 (MQ=255)
tttCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGc < 2:187521/207‑1 (MQ=255)
aTTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGGAGCCTTGTCCAGGG‑GTCAGACGGGGGACGCATCCGTGCCCCCCCCCGCGGGGCCTGTTCGGTGTGCCCCGGCggg > 2:451499/1‑227 (MQ=17)
aTCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCAATGTCCAGGG‑TTCAACCCGGGTACGCATCGGTGCCCCCCCCGGCGTGGGCGGTTCCGGGTGGCCCGGCgggggggacgccg > 2:699990/1‑219 (MQ=14)
aCAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑GTCAGCCGGGGGACGCATCGGGGCCCCCCCCGGCGTGGCCTGGTCGGGGGGGCCCCGCGGGGGGACGTCCCGccc > 2:555528/1‑226 (MQ=14)
gATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGCCGCAGTATGGCGGGAACAATGTCCGCGG‑TTCTGGCCGGGGACACGCCGGGGACCCCACCCCGGCGGCCTGCTCGGGGGGGCCCCGCCGGGCGGCGTCCCGccccccc > 1:542384/1‑223 (MQ=9)
ctcACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAG‑AAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGG‑GTCAGCCGGGGGGGGCATTGGTGCCCCCCCCGGCGTGGGCCTTTCGGGGGGGCCCGGGGGGGGGGGGGCCCGCCACCCggggctg > 1:3463/1‑223 (MQ=14)
ctcACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTTGCCCGGG‑TTCAGCCGGGGAACGCATCGGGGCACCCCCCGGCGTGGCCCGTTGCGGGTGCCCCGCCGGGGGGCGCTCCGGCCACCCggccca > 2:90894/1‑224 (MQ=14)
aCTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACg < 1:8854/156‑1 (MQ=255)
tgCTTTCTTATAAATCTGTAGGGTTTCGCCTGGCAGCAGACAAATAGCCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCgctgct < 2:63720/227‑1 (MQ=255)
ggCTTTTTTAAAAATCTGTTGGGTTTCGCCTGTCAGCACGCAAATAACCCGATAACACAAGGAGGAGCAGATGAGCCGAAACCCGGCGGCGCGTAAGGGCGACATGACGCAGTATGGCGGTAGCATTGCCCAGGG‑TTCAGCCGGGGTACGCCTCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCgctgct < 1:90894/228‑1 (MQ=14)
ttCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCCCGTCAGGGCGACATGACGCAGTATGGCGGTAGc > 2:147378/1‑120 (MQ=255)
tCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCCCGTCAGGGCGACATGACGCAGTATGGCGGTAGCa < 1:147378/120‑1 (MQ=255)
cTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGc < 2:74072/228‑1 (MQ=255)
gggTTTCGCCTGTCAACAGACAAAAAACCCGAAAAAACAGGGATGAGCAGATGGGGGGAAAACCGGCGGCGCGTCAGGGCGGCCTGACGCAGTATGGCCGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCg < 1:555528/228‑1 (MQ=14)
tCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCa < 2:356526/178‑1 (MQ=255)
cccGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTgcccggcc < 2:674505/228‑1 (MQ=255)
aTGAGCAGGTGAGCGGAAACCCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCAtt < 2:145935/227‑1 (MQ=255)
aTGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCCCCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGGGACGTCCGGCCATCCGGTCCATCCGCTGCTCGGTGCCAAGGTGCTTCCCGGTGAAACCGAAATACCCCCTCCCGGCCCCCCGCCCTTCAtt > 2:241749/1‑227 (MQ=14)
gtgggccgAAACCCGGCGGCCGGGCAGGGCGACATGGCGCGGTATGGCGGTGGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATGGGGGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAACCCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGc < 1:699990/221‑1 (MQ=14)
aggAGCGGAAAACCGGCGGGGCGTCAGGGCGAAATGACGCAGTATGGCGGTAACATTGGCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGc < 2:142453/226‑1 (MQ=255)
|
CAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGC > NC_000913/729399‑729810
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
