Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 429,854 T→G 12.5% V117G (GTG→GGG)  secF → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913429,8540TG12.5% 343.8 / 3.3 134V117G (GTG→GGG) secFSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base T (39/71);  new base G (20/4);  total (59/75)
Fisher's exact test for biased strand distribution p-value = 2.23e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

TTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGAT  >  NC_000913/429620‑430088
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           
tttttttGGGGGTGCGCGGCTTTAACTGGGGGCTGGATTTCCCCGGTG‑GTAAGGTTATTGAAATTACGCTCGAAAAACCGGCGGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGAtt                                                                                                                                                                                                                                         <  2:768791/233‑1 (MQ=255)
        ggCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGATCTTAAGGTGATTAACGGAt                                                                                                                                                                                                                                  >  1:1050663/1‑238 (MQ=255)
          cGTGGGCGGTTTTACTGGGGGGGTGGATTTCACGGGGG‑GTACGTTTATTGAAATTACGCTCGAAAAACCGGCGGAAATGGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTGGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATcc                                                                                                                                                                                                                                <  2:147485/238‑1 (MQ=255)
            tGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCACCCCGGTTCTGAAGGGGATTAACGAATccccc                                                                                                                                                                                                                             >  1:112747/1‑239 (MQ=255)
             gcgcGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGGAACCCGGGTCTGAAAGGGAATaaaagaaacaaca                                                                                                                                                                                                                            >  2:636025/1‑228 (MQ=255)
              cgcgGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGAATccaccac                                                                                                                                                                                                                           >  1:656611/1‑238 (MQ=255)
              cgcgGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGCCAGGGGTTGGGCAGCCAGGTTCTGAAGGTGGTTAACGAATccacca                                                                                                                                                                                                                            >  1:350379/1‑238 (MQ=255)
                cgGCTTTAACTGGGGGCTGGATTTCGCCGGTG‑GTACGGTTAGTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAAt                                                                                                                                                                                                                          <  1:55649/238‑1 (MQ=255)
                    tttAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGa                                                                                                                                                                                                                      <  1:522954/238‑1 (MQ=255)
                      tAACTGGGGGCTGGATTTCACCGGTG‑GTAAGGTTATTGAAATTACGCTCGAAAACCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATg                                                                                                                                                                                                                   <  1:912269/239‑1 (MQ=255)
                      tAACGGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATg                                                                                                                                                                                                                   <  1:446969/239‑1 (MQ=255)
                           gggggCGGAATTTCACCGGTG‑GTAACGTTATTGAAATTACGTCCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATgcagca                                                                                                                                                                                                              <  1:1080675/239‑1 (MQ=255)
                             gggCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGGAGCCAGGTTTTGAAGGGGCTTAACGAATCCACCAATAAGAATgcagccgg                                                                                                                                                                                                            >  1:1155822/1‑236 (MQ=255)
                             gggCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCGGAAGGCGAAACCGCCGGGCAGAGGGTGGGCAGCCCGGATCTTACGGGGAGTAAAGAAAccccacaaaaagattcagcagg                                                                                                                                                                                                            >  2:1260292/1‑219 (MQ=255)
                              ggCTGGATTTCACTGGTGTGTA‑GGTTATTGAAATTCCGCTCGAAAAACCGGCTGAAAGTGACTTAATGCGTGATGCATTGCAAAAATCCGGTTTTGAAGAGCCGATGCTGCAAAACGTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                                                            <  2:1080792/238‑1 (MQ=255)
                              ggCTGGATTTCACCGGTG‑GTACGGTTATGGAAATTACGCTCGAAAACCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCTGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                                                            <  1:591164/238‑1 (MQ=255)
                                ttGGATTTCCCCGGTG‑GGACGGTTATTGAAATTATGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGAGGCATTGCAAAAGGCCTGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGa                                                                                                                                                                                                          <  1:1345623/237‑1 (MQ=255)
                                gtGGTTTTCCACGGTG‑GGTCGGTTATTGAAATTACGCCCGAAAAGCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGTTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGa                                                                                                                                                                                                          <  2:89840/237‑1 (MQ=255)
                                 tGGTTTTCCCCGGTG‑TTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGAAGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAg                                                                                                                                                                                                        <  2:1001828/239‑1 (MQ=255)
                                             gtg‑gtACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGGGAAGCGTTTTGAGTt‑c                                                                                                                                                                                           >  1:228065/1‑239 (MQ=255)
                                                 gttctgTTATTTAAATTACGCTCAAAAAACCGGCTAAAATTGGCGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTt‑cgt                                                                                                                                                                                         <  2:89499/233‑1 (MQ=255)
                                                 gtACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATcca                                                                                                                                                                                                                               <  1:958437/201‑1 (MQ=255)
                                                 gtACGGTTATTGAAATTACGCACGAAAAACCGGGTGAAAGTGACGTAAGGCGTTATGCATTGGAAGAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTt‑cgt                                                                                                                                                                                         <  2:1070137/238‑1 (MQ=255)
                                                    cGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGt                                                                                                                                                                                     <  1:374667/239‑1 (MQ=255)
                                                        tATTGAAATTACGCTGAAAAACCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTGGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCg                                                                                                                                                                                  <  1:527346/238‑1 (MQ=255)
                                                         aTTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCGGTGAAGCGGATTGAGTT‑CGTCGGTCCGa                                                                                                                                                                                 >  1:89499/1‑238 (MQ=255)
                                                         aTTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGGCGCCAGGTTTTGAAGGTGATTAAAGAAACCCCCAATCACAAATCAGCAGTGGAggcgattgggattgtcgggtcgag                                                                                                                                                                                 >  2:927830/1‑216 (MQ=255)
                                                           tGAATTTACTCTCGAAAAACCGTCGGAATTTGCCTAAATGCGTTAGGACTTGCAAAAAGCCTGTTTTGAAGAGCCGAAGCTTCAAAACGTTGGTAGCAGCCATGGCATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCTCCAATCAGAATGCAGCAGTGAATCGTATTGAGTT‑CGTCGGTCCGAGCg                                                                                                                                                                              <  2:437715/239‑1 (MQ=255)
                                                             aaattcccgttaaaaaaCCGGGTGAAATTGGCTTAATGGGTGATGAATTGCAAAAAGGCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCCGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTg                                                                                                                                                                            <  2:534588/227‑1 (MQ=255)
                                                                 tACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCCATCAGAATGCAGCAGTGAAGCGGATTGAGTT‑CGTTGGTCCGGACGTggggg                                                                                                                                                                        >  1:617551/1‑239 (MQ=255)
                                                                  acgagagAAAAACCGGCTGAAATTGACGTAATGCGTGATTCATGGCAAAAAGCCGGGTTTGAAGAGCCGATGCTGCAAAGCTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCTGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGc                                                                                                                                                                       <  1:406150/233‑1 (MQ=255)
                                                                     cTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCCGCCAGGTTCTTAAGGTGATTAACGAATCCCCCAATCAGAATGCAGCGTTGACACGAATTTGGTT‑CTTCGGTCCGAGCGGGGGGGccg                                                                                                                                                                     >  1:534588/1‑236 (MQ=255)
                                                                     cTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCCGGCTCTGAAGGTGTTTAACGAATCCCCCAATAAGAATGCAGCAAGGGAGCGGATTGGGTT‑CGTCGGTCCGGGCGTgggggacg                                                                                                                                                                     >  2:266963/1‑235 (MQ=255)
                                                                       cGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGAcc                                                                                                                                                                  <  1:407067/239‑1 (MQ=255)
                                                                        gAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCGAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCCGGGGTTGGGCAGCCAGGTTCTGAAGGGGTTTAACGAATCCACCACACCTAATACAGCAGTGAAGCGAATGAAGTT‑CGTCGGTCCGAAGGGGGGGGGAGAcca                                                                                                                                                                 >  2:539820/1‑238 (MQ=255)
                                                                                  cTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCCGGGGTTGGGCAGCCAGGGTTTGAAGTGGTTTAACGAATCCACCAATCCTGATGCCGCCGGGGAAGGTATTGGGTT‑TGGGGGTCCGAGCGTGGGGGGAGGCCTGGCGCAAAcc                                                                                                                                                       >  2:1155132/1‑239 (MQ=255)
                                                                                    gAAATTTGCGTAATGCGTGATGCATTGCAAAAAGCGGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCgg                                                                                                                                                     <  2:656611/239‑1 (MQ=255)
                                                                                         ggccGTATTGCGTGTTGGATTGAAAAAAGCGGGTTTTGAAGAGCCGTTGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGa                                                                                                                                                <  2:80500/236‑1 (MQ=255)
                                                                                           gcttaaggggttacgtaTTGAAAAAACGCTGTTTTGAGGGGGCGGTGGTGCAAACCTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATg                                                                                                                                              <  2:350379/223‑1 (MQ=255)
                                                                                           ctgtaagtggtggtggtttGAAAAAGGCCGGTTTTGAAGGGCCGATGCCGCAAACCGTGGGTAGCAGCCATGACAGCATGGTCCGTATGCCGGCTGCGGAAGGCGAAACCGTCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATg                                                                                                                                              <  2:228065/222‑1 (MQ=255)
                                                                                           aCGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATg                                                                                                                                              <  1:81930/239‑1 (MQ=255)
                                                                                            cGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGTCATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGaa                                                                                                                                                                                                                     >  1:65942/1‑168 (MQ=255)
                                                                                                aTGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGGGTT‑CGTCGGTCCGAGCGGGGGGGGAGACCTTGCGCAAACCggggggaggggggg                                                                                                                                         >  2:1170773/1‑227 (MQ=255)
                                                                                                     tGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCCATCAGAATGCAGCAGTGAAGCGGATTGAGGT‑TGTCGGGCCGAGCGTGGGGGCAGACCTTGGCCAAACCggggggggggggggggggg                                                                                                                                    >  2:1113485/1‑222 (MQ=255)
                                                                                                       aTGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTAGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAGAATGCAGAACTGAAGCGTATTAAGTT‑TGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGGTGGCGTTGTTggga                                                                                                                                  >  1:116768/1‑237 (MQ=255)
                                                                                                        tGCATTGCAAAAAGCCGGTTTTTAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAg                                                                                                                                 <  1:1345026/239‑1 (MQ=255)
                                                                                                            ttGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATTACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgct                                                                                                                             <  1:1014849/239‑1 (MQ=255)
                                                                                                            ttGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAgcgc                                                                                                                              <  1:221269/238‑1 (MQ=255)
                                                                                                             tttaagggacaggTTTTGAAGGGCCGAGGCTGCAAAACTTTGGTAGCAGCCATGGCATCAGGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                                            <  2:1375679/228‑1 (MQ=255)
                                                                                                             ttcAAAAAGCCGGTTTTGAAGGGCCGATTGTGCAAAACTTTTGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                                            <  2:75142/237‑1 (MQ=255)
                                                                                                             tggaaaaaGCCGATTTTGAAAAGCCGGTGCTGCAAAACTTTGGTAGGAGCCAGGACATCAGGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGGAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                                            <  1:776400/236‑1 (MQ=255)
                                                                                                             tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGa                                                                                                                                                                    >  1:1069835/1‑199 (MQ=255)
                                                                                                             tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                                            <  1:828996/239‑1 (MQ=255)
                                                                                                             tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGGATTTAGTT‑CGTCGGTCCGACCGTGGGGGCAGACCTTGCGCAAACCGGGGCGATGGCGTTGATGGCacggct                                                                                                                             >  1:379159/1‑233 (MQ=255)
                                                                                                             tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAGAATGCCGAAGTGAAGAGTATTGAGTT‑CGCCGGTCCGACCGTGGGGGCAGACCTTGCGCAAACCGGGGCGATGGGGGTGCTGGCAGGgct                                                                                                                             >  1:51808/1‑238 (MQ=255)
                                                                                                             tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGAGTTGGGCAGCCAGGTTCTGAAGGTGATTAAGGAATCCACCAATCAGAATGCCGCCGTGAAGCGTATTGAGTT‑CGTCGGTTCGAGCGTGGGGGCAGACCTTGCGCAAACCGGGGCGATGGCGTTGAAGGccggggt                                                                                                                             >  1:1134829/1‑232 (MQ=255)
                                                                                                             tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAAGTGTTGGGCAGCCAGGTTCTGTAGGGGATTAACGAATCCACCAAACAGAATGCAGACGTGAAACGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGGCGACCTGGCGCAAACCGGGAGGAAGGCGTTGATGGccaggcgg                                                                                                                            >  2:904128/1‑232 (MQ=255)
                                                                                                             tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGCCCGGTGTTGGGCAGGCAGGTTCTGAAGGTGATTTACGAATCCCCCAATCAGAATGCAGCACTGAAGGCGATTAGGTTACGT‑GGTCCGCGCCGGGGGGCAGCCCATGCGCAAACCGGTggagaggggctgaggggagggcgg                                                                                                                            >  2:224312/1‑216 (MQ=255)
                                                                                                                  aaaGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGGAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAGAATGCAGCAGGGAAGCGCATTGGTTT‑CTTCGGTCCGAGCGTGGGGGCAGGCCTTGCGCAAAACGGTGCGATGGGGTTGATGGGAGGgctgcgg                                                                                                                         >  1:741959/1‑235 (MQ=255)
                                                                                                                  aaaGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATcca                                                                                                                                                                                                                               >  1:15139/1‑136 (MQ=255)
                                                                                                                  aaaGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATcca                                                                                                                                                                                                                               <  2:15139/136‑1 (MQ=255)
                                                                                                                  aaaGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCCGCAGTGAAGCGTATTGAGTT‑CGTTGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGGTGGCGTTGATTGTAGGgctgcgg                                                                                                                         >  1:639274/1‑235 (MQ=255)
                                                                                                                       cGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGGAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTAt                                                                                                                    <  1:326311/237‑1 (MQ=255)
                                                                                                                       cGGTTTTGAAGGGCCGAGGCTGCAACACTTTGGTAGCAGCCATGACATCATGGGCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATc                                                                                                                   <  2:323157/238‑1 (MQ=255)
                                                                                                                       aggTTTTGAAGGGCCGGTGCGGCAAAACTTTGGGAGCCGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATcc                                                                                                                  <  2:245305/238‑1 (MQ=255)
                                                                                                                                agggCCGGTTCTTCAAAATTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACg                                                                                                         <  2:379159/236‑1 (MQ=255)
                                                                                                                                agagCCGATGTTGTAAAACTTTGTGAGCAGCCATGACATCATGGTCCGTATGCCGCCGGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACg                                                                                                         <  2:288452/239‑1 (MQ=255)
                                                                                                                                 gagCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCg                                                                                                                                                                              <  1:752580/169‑1 (MQ=255)
                                                                                                                                    ccGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGGGCTGCTGTCTATcccctgtcccttata                                                                                                     >  1:1375679/1‑226 (MQ=255)
                                                                                                                                    ccGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGGGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTact                                                                                                     >  1:245305/1‑237 (MQ=255)
                                                                                                                                    ccGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGGATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGGGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGGACGTggg                                                                                                     >  1:162456/1‑239 (MQ=255)
                                                                                                                                    ccGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCCATCAGAATGGAGCAGTGAAGCGGATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGGTGGCGGTGATGGAGGCGCGCCGGGCTATCCTCGTGGACGGAgg                                                                                                     >  2:431906/1‑239 (MQ=255)
                                                                                                                                         gctgcAAAGCTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGa                                                                                                                                                                                                                                    <  1:593714/108‑1 (MQ=255)
                                                                                                                                         gctgcAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGa                                                                                                                                                                                                                                    >  2:593714/1‑108 (MQ=255)
                                                                                                                                                               cctggcatcatGGTCCGTAGGGCGCCTGCTGAAGGCGAAACCGGGGGTCAGGGGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTggcgg                                                                           <  1:278254/233‑1 (MQ=255)
                                                                                                                                                                atggcttctttgttcgtcagccgcaTGGTGAAGGCGAAACCGGGGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCa                                                                         <  2:1294938/220‑1 (MQ=255)
                                                                                                                                                                    aatcatTGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAgggg                                                                     <  1:927830/238‑1 (MQ=255)
                                                                                                                                                                        atGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGT‑GGGGCAGACCTTGCGCAAACCGGTGCGCTGGCGTTGATGGCAGGGCTGCTGTCCATCCTCGTGTACGTGGGTTTACGCTTTGAGTGGGGACTGGGGGCCGgggggggt                                                                >  1:69086/1‑237 (MQ=255)
                                                                                                                                                                        atGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTCCGTAGGTTTCCGCTTTGCGGGGGGACTGGGGGCCGggggggg                                                                 >  1:1041499/1‑238 (MQ=255)
                                                                                                                                                                               gTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCCGACGGGGGGGGTAttcgc                                                          >  1:702116/1‑236 (MQ=255)
                                                                                                                                                                                tATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGTTGGCAGCGCGGCTGCCTATCCTCCGGGACGAGGGTTTCCGCTTTGAGGGGCGGCGGGGGGCAGggggggggtattggt                                                         >  2:73271/1‑230 (MQ=255)
                                                                                                                                                                                tATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTa                                                                                                                     >  1:912423/1‑179 (MQ=255)
                                                                                                                                                                                      gccTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGGAGGGGGGGGTATTTGGCTGgcg                                                    >  1:1275795/1‑238 (MQ=255)
                                                                                                                                                                                      cctgttgtaaGGCGAAACCGGGTGTCAGGTTTTGGGCGGCCAGGTTCTGAAGGTGATTAACGAACCCACCAATCCGAATGCAGGTGGGAGGCGTATTGGGTT‑TGTCGGTCCGAGCGGGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGgcgc                                                   <  2:447594/231‑1 (MQ=255)
                                                                                                                                                                                       ctcggtgAGGGCGAAACCGGCGGTCCGGTGTTGGGCAGGAGGGTTCTGAAGGTGGTTTATGGATCCACCAATCAGAATGCGGCAGTGAAGCGGATTGAGGT‑CGTTGGTCCGAGCGTGGGGGCAGCCCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCGCGTGTACGTAGGGTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCa                                                  <  2:1234489/234‑1 (MQ=255)
                                                                                                                                                                                       cctggctaaGGCGAAACCGGGGGTCAGGTGGTGGGCAGCAAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCa                                                  <  1:481496/232‑1 (MQ=255)
                                                                                                                                                                                       ccTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTt‑cgt                                                                                                                                                                                         >  2:913258/1‑104 (MQ=255)
                                                                                                                                                                                                       ccGGCGTTCAGGTGTTGGGCCGCCAGGTTCTGAAGGTGATTAACTAATCCCCCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACg                                  <  2:390172/239‑1 (MQ=255)
                                                                                                                                                                                                         ggctgTCAGGGTTTGGGCAGCCAGGTTCGGAAGGTTATTCACGAATCCACCAATCAGAAGGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCt                                <  1:1260292/239‑1 (MQ=255)
                                                                                                                                                                                                            gggcatgtgtTGGGCAGCCAGGTTCGGAAGGGGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGGT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCGGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTgg                              <  2:562247/232‑1 (MQ=255)
                                                                                                                                                                                                                aGGTGTTGGGCAGCCAGGTTCTGAGGGGGATTAACGAATCCACCAATCAGGATGCAGCAGTGAAGCGTATGGAGTT‑CGTCGGTCCGAGGGTAGGGGCAGTCCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTAtt                         <  2:1134829/239‑1 (MQ=255)
                                                                                                                                                                                                                  gtgtTGGGCAGCCAGGTTTTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGGGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTAttt                        <  2:112747/238‑1 (MQ=255)
                                                                                                                                                                                                                    gtTGGGGAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATGAGAATGCAGCAGTGAAGCTTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGt                     <  1:1078746/239‑1 (MQ=255)
                                                                                                                                                                                                                        ggCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGgcgc                                                   <  2:709397/205‑1 (MQ=255)
                                                                                                                                                                                                                        ggCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGgcgc                                                   >  1:709397/1‑205 (MQ=255)
                                                                                                                                                                                                                              cgggTTTTGAAAGGGATTAACGAAACCACCAATCAGAATGCAGGAGTGAAGCGTGATGGGTG‑CGTCGGTCCGAGCGTGGGGGCAGCCCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTTTCGTTAtt              <  2:116768/234‑1 (MQ=255)
                                                                                                                                                                                                                                     tGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCCGGGGTGGTTATTGGTCTGGCGCACGACGGTATCCTTACGCTGGGGATTTTTTCGTTATTCCATTTCgag    >  1:1344821/1‑239 (MQ=255)
                                                                                                                                                                                                                                      gAAGGTGATTAACGAACCCACCAATACGAATGCAGCAGTGAAGCGGTTTGGGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCgaga   <  2:1275795/239‑1 (MQ=255)
                                                                                                                                                                                                                                       aaGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCCGGGGTGGTTATTTGCCGGGCGCCCGACGTTATCATTTCGCTGGGGATTTTGTGTTTTTTCCATATCGAGAt  >  2:86665/1‑239 (MQ=255)
                                                                                                                                                                                                                                       aaGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGGGGTTATTGCGCTGGGGCACGAGCTTTTCTTTACCCTGGGGATTTTGTCGGTTTTcctaatccggtt  >  1:32681/1‑229 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           
TTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTT‑CGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGAT  >  NC_000913/429620‑430088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A