Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,537,275 T→C 11.8% E12G (GAG→GGG)  narW ← nitrate reductase 2 (NRZ), delta subunit (assembly subunit)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,537,2750TC11.8% 374.6 / 4.5 142E12G (GAG→GGG) narWnitrate reductase 2 (NRZ), delta subunit (assembly subunit)
Reads supporting (aligned to +/- strand):  ref base T (60/62);  new base C (10/10);  total (70/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

GCCAGCAGGTCCACCATTGCCTGGCCGCGATC‑GCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCG  >  NC_000913/1537044‑1537513
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                
gCCAGCAGGTCCACCATTGCCTGGCCGCGATC‑GCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGGTCAACGCCCACGCGTCCTCCTTGCATTCCCACACC‑AGCTCGTCTCGAAACTCCGTC‑aa                                                                                                                                                                                                                                          >  1:1088530/1‑239 (MQ=255)
 ccAGCAGGTCCACCATTGCCTGGCCGCGATC‑GCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCCTCGGCGCGTCGCGGCGGATCAACGCCAGCGCTTCCTCCTTGCATTTCCCAAAC‑AGACCGGCCGGATACCCCATcca                                                                                                                                                                                                                                           >  1:1319579/1‑236 (MQ=255)
       tgTCCCCCATGGCCTGGCCG‑GAGCTGCGGGACTCGGGATGAACCTGTTCGAACATGAGCAGGTAC‑GTGGTGCGCCCGCGGGCAAACACTTCGGACCATTCGGCCTGTTTATCCAGCAGCGGGGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCCTCCTCCTTGCATTCCCACAAC‑AGCTGGTCCGGATACTCCATC‑AACAGGCCg                                                                                                                                                                                                                                   <  2:1029318/238‑1 (MQ=255)
         tCCACCATTGCCTGGCCGCGATC‑GCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGGTCAACGCCAGCGCGTCCCCCTTGCATTCCCACAAC‑AGCCCGTCCGGAAACTCCCTC‑ACAAGGCCGAt                                                                                                                                                                                                                                 >  1:387994/1‑239 (MQ=255)
         tCCACCATTGCCTGGCCGCGATC‑GCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCCCGTTTAGCCGGCTGCGGGGGAAATCCGTAAACATCTGCGCGTCTCGGCGGACCACCGCCCCCGCGTCCTCCCTTCCTTCCCCCAAC‑CGCCCCCCCCGAAACCCCATC‑CACAGAcccac                                                                                                                                                                                                                                 >  2:1150908/1‑236 (MQ=255)
          cccccaTTGCCTGGCCGCGATC‑GCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGAt                                                                                                                                                                                                                                 <  1:602832/235‑1 (MQ=255)
          ccaccaTTGCCTGGCCGCGATC‑GCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGAt                                                                                                                                                                                                                                 <  1:521003/238‑1 (MQ=255)
          ccaccaTTGCCTGGCCGCGATC‑GCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGAt                                                                                                                                                                                                                                 <  1:1146386/238‑1 (MQ=255)
                  gCCTGGCCGCGATC‑GCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGGGCGTCGCGGCGGGTCCAGGCCCGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGCTACTACATC‑AACACGCCCATCACTTTGAg                                                                                                                                                                                                                        >  1:1289012/1‑239 (MQ=255)
                  gCCTGGCCGCGATC‑GCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGGTCAACGCCAGGGCGTCCTCCTTGCATTCCACCAAC‑AGCTCGTCCGGATACTCCATA‑AACAGGCCGacccccttaag                                                                                                                                                                                                                        >  1:1357808/1‑229 (MQ=255)
                   ccTGGCCGCGATC‑GCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGGAAGCATCGGCGCGTCCCGGCGGAGCAACACCAGCCCGGCCCCCTTGCATTACCCCAAC‑CACCCATCCGGATAACCCACC‑AACAAGCCGacacccttttgg                                                                                                                                                                                                                       >  2:622831/1‑228 (MQ=255)
                     ggcccGCGCTC‑CGGGGCCTCGGCATTAACATGTTCGAACAGCAGCAGCGAC‑GTGGGGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGAt                                                                                                                                                                                                                     <  2:387994/236‑1 (MQ=255)
                       gCCGCGATC‑GCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCCGGTTGTGGGGGAAATCCGTAAGCATCGGCCCGTCCCGGCGGACGAACCCCCGCGCGTCCTCCTTGCATTCCC‑CAACAAGCCCGTCCGGGAACCCCACCAAACAGCCCG‑CCCCTTTGAGGaaa                                                                                                                                                                                                                    >  2:899976/1‑236 (MQ=255)
                        ccGCGATC‑GCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGGGAAATCCGTAAGCATCGGCGCGTCGCGGCGGGTCCACGCCAGCGCGTCCTCCTTGAATTCCCCCACC‑AACCCGCCCGGGTACTCACCC‑AACAGCCCGCTACCCTTGAGGactt                                                                                                                                                                                                                   >  2:1293148/1‑235 (MQ=255)
                          gcgATC‑GCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGAGCGTCCCCCTTGCATTCCCACAAA‑CGCTCGTCCGGATACTCCACC‑AACAAGcccaaaccatttaggaccggca                                                                                                                                                                                                                >  2:434277/1‑219 (MQ=255)
                            gATC‑GCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCCCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTTAGAATCTGCatt                                                                                                                                                                                                              >  1:20311/1‑239 (MQ=255)
                               c‑gcgGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTATTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCattatt                                                                                                                                                                                                           <  1:277635/239‑1 (MQ=255)
                                    ggacgcggtcaggacgttgtgcgaCAGCAGCAGGGGCTGGGGTGCGCCGGC‑TCCAAACACTTCGCCCCTGTCGGGCTGTTTCTCCAGCAGCGGCGCGTTAAGCAGGTGGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTTCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGcc                                                                                                                                                                                                       <  2:454991/216‑1 (MQ=255)
                                     gACTCGGCATGAACATGTTCGAACCGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTACCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGcc                                                                                                                                                                                                       <  1:119630/238‑1 (MQ=255)
                                     gACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGcc                                                                                                                                                                                                       <  1:754103/238‑1 (MQ=255)
                                            cATGAACATGTCCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACCCTGCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCt                                                                                                                                                                                                <  1:50184/238‑1 (MQ=255)
                                               gAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGGGTCCTCCTTGCATTCCCACAAC‑AGCCCGTCCGGATCCTCCCTC‑AAACGGCCGATCACCTTAGGGATCTCCATTTTTCGCCCTCCGCCTTG‑t                                                                                                                                                                                           >  2:1065606/1‑239 (MQ=255)
                                               gAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTTGCCCTCCGCTTTg                                                                                                                                                                                             >  1:218224/1‑238 (MQ=255)
                                                aaCATGTTCAAACAGCAGCAGCGCG‑GTGGGGCGCCCGGGGTCAAGCACTTCGCACCAGTCGGCCTGTTTATCCAGCAGCGGCGCGTGAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCGCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCt                                                                                                                                                                                                     <  2:1040029/229‑1 (MQ=255)
                                                   cttttCGAACAGCCGCCGCGAC‑GTGGTGCGCCCGCCGTCAAACCCCTCGCCCCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCACCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑Tcgcg                                                                                                                                                                                       <  2:219215/236‑1 (MQ=255)
                                                   attttCGAACCGCAGCCGCGTC‑GTGGGTCGCCCGCGGTCAAACCCTTCGCCCCGTTCGGCCTGTTTATCCAGCAGGGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑Tcgcg                                                                                                                                                                                       <  2:1352136/236‑1 (MQ=255)
                                                     gTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑ACCAGGCCGCTCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCgcgc                                                                                                                                                                                      >  1:104738/1‑238 (MQ=255)
                                                      ttccaaccggggcagcCAC‑TTGGGGCGCCCGCGGTCAAACCCTTCCCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAAGCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCAc                                                                                                                                                                                    <  2:218224/228‑1 (MQ=255)
                                                        cGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGCTCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACt                                                                                                                                                                                   >  1:237792/1‑238 (MQ=255)
                                                          aaCAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGGTCAACCCCAGCGCGTCCTCCTTTACTTCCCACCAC‑AGCCCGTCCGGATACCCCCTC‑aa                                                                                                                                                                                                                                          >  2:663478/1‑182 (MQ=255)
                                                          aaCAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑Tc                                                                                                                                                                                          >  1:1040029/1‑229 (MQ=255)
                                                          aaCAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCAGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCCCAAC‑AGACCGTACGGACACCCCATC‑AACAGGCCGATCACCTTGAGGATAGGAATGATTCGCCCCCCCATTTT‑GCGCGGACTTCg                                                                                                                                                                                >  2:575055/1‑239 (MQ=255)
                                                                agcagcGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGCTCACTTTTAGGATCTGCATTATTCGCCCTCCGCTTTT‑TCGCGCCCTTCGGTGATg                                                                                                                                                                          >  1:910792/1‑239 (MQ=255)
                                                                         cggggggcccccGCGGTCAAACCCTCCGCCCCATTCGGGCTGTTTATCCAGCGGCGGCGCGTTAAGCAGGTTGGGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGc                                                                                                                                                                  <  2:104738/230‑1 (MQ=255)
                                                                          gtggtgCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGAACAACGCCAGCGCGGCCTCCTTGCATTCCCACACC‑AGCTCGTCCGGAACATCCACC‑AACATGCCGGTGACCTTCAGGAACCGCCATATTCGCCCCTCCCTTTGTTCGCGCACTCCGG‑GATGTTGATggg                                                                                                                                                                  >  2:8097/1‑237 (MQ=255)
                                                                             gtgCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGCTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAACGCGTCCTCCTTGCATTCCCACAAC‑AGCCCGTCCGGATACTCCGTC‑ACACGGCCGCTCACTTTGCAGAACTGCAGTCTTTGCCCACCCCTTTG‑TCGCGCACCTGTGTGGTGtttagtgcaccg                                                                                                                                                              >  2:782642/1‑229 (MQ=255)
                                                                                 gcCCCCGGTCAAACCCTTCGCCCCATTCGGCCTTTTTACCCAGGAGCGGCGCGTTAAGCAGGTTGGGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATa                                                                                                                                                           <  2:1336153/238‑1 (MQ=255)
                                                                                    cgggggtaaccccTTCGCACCTTTCCGCCTTTTTAACCAGCAACGGCGGTTTAAGCAGGTTGTGGGGTAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCCTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTGCGGTGATGTTGATGGCATCGATACGGc                                                                                                                                                       <  2:20311/227‑1 (MQ=255)
                                                                                    cggggTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGc                                                                                                                                                       <  1:1065606/236‑1 (MQ=255)
                                                                                    cggcggcAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGGGGCGCGTTAAGCCGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGc                                                                                                                                                       <  1:660268/233‑1 (MQ=255)
                                                                                    cgcgGTACAACCCTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCCTCCGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGc                                                                                                                                                       <  1:1234559/239‑1 (MQ=255)
                                                                                    cgcgGCCAAACCCTTCGCCCCATTCGGCCTGGTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGc                                                                                                                                                       <  1:683675/239‑1 (MQ=255)
                                                                                           gcaggcctCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTg                                                                                                                                                  <  1:313429/230‑1 (MQ=255)
                                                                                                   cGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCCCTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGt                                                                                                                                        >  1:218136/1‑239 (MQ=255)
                                                                                                   cGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGGGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGTCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCAATATTCGCCCTCCGCCTTG‑TCGCGCACTTCGGTGATGGTGATGGGATTGAAACGGCTACTGTTGAACAggg                                                                                                                                        >  2:1019921/1‑238 (MQ=255)
                                                                                                      aCCAGTCGGCCTGTTTATCCGGGAGGGGGGCGGTAAGAAGGTTGGGGGTGAAACCCGTAAGCACCGGCGCGTCGGGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTg                                                                                                                                      <  2:622618/238‑1 (MQ=255)
                                                                                                         aTTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTc                                                                                                                                                                                                                                                 >  1:863440/1‑130 (MQ=255)
                                                                                                          ttCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGAAACTCCATC‑CACAGGCCGGTCACTTTTAGGATCTGCATTATTCGCCCTCCGCTTTT‑TCGCGCACCTTGGGGAGGTTTCTGGCTTCGCTTAGGCCACCGTTGGAAAgggtcttgc                                                                                                                                  >  2:313429/1‑231 (MQ=255)
                                                                                                           tCGGGCTGGTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTa                                                                                                                                <  1:1098641/239‑1 (MQ=255)
                                                                                                           tCGGCCTTTTTTCCCAGCAGCGGCCGGTTAAGCAGGTTGGGGGTGAAATCCTTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTa                                                                                                                                <  2:49117/239‑1 (MQ=255)
                                                                                                           tCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCCTTATTCGCCCTCCGCTTTT‑TCGCGCCCTTCGGTGTTGTTGTTGGCTTCGATACGGCTACTGTTGAACCGGTTGAAttt                                                                                                                                 >  1:622618/1‑238 (MQ=255)
                                                                                                           tCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTa                                                                                                                                <  1:899976/239‑1 (MQ=255)
                                                                                                           tCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGGCCGGATACCCCATC‑AACAGGCCGATCACTTTTGGGATCTGCATTATTCGCCCCCCCCTTTG‑TCGCGCCCTTCGGTGAGTTTGATGGCATCCATACGGCAAACGTTGAAAAGGTGGAAATTa                                                                                                                                >  2:124189/1‑239 (MQ=255)
                                                                                                           gcGGCCTGGTTATCAAGGGGCGGCGCGTTAAGGAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGAGCAACGCCAGCGCGTCCTCCGTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTa                                                                                                                                <  2:879678/238‑1 (MQ=255)
                                                                                                             acggggTTTATCCCGGAGGGGGGGGTGACGCGGGTTGTCGGTTAACTCCCTAAGCTAAGGCCTGTCGCGGCGGATCAACGCCAGAACGTCCCCCCTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCTGATCACTTTGAGGATGTGCATTATTCGCTCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTa                                                                                                                                <  2:520131/232‑1 (MQ=255)
                                                                                                                cTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGt                                                                                                                            <  1:337442/238‑1 (MQ=255)
                                                                                                                  gTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCg                                                                                                                         >  1:1142156/1‑239 (MQ=255)
                                                                                                                   tttATCCAGGAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGTCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGa                                                                                                                        <  1:1019921/239‑1 (MQ=255)
                                                                                                                            cagcGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTTTTGTACAAGTTGAATTTACTGTCCGaaaccgttt                                                                                                                >  1:520131/1‑231 (MQ=255)
                                                                                                                             agcGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCCGGGCGTCCTCCTTGCATTCCCACAAC‑AGCGCGGCCGGGTACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCCCGCACTTCGGTGTTGTTGTTGGCATCGTTACGGCTACTTTTTAAAAGTGTGAATTAACttgcacaaaccgggca                                                                                                               >  2:485322/1‑223 (MQ=255)
                                                                                                                             agcGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCAAACCGTGGCa                                                                                                               >  1:497759/1‑238 (MQ=255)
                                                                                                                               cggcgCGTTAAGCAGGTTGTGGGTG‑AATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGCGATGTTGATGGCATCGATACGGCTACTGTTGAACCGGTTGAATTTACTGTCCGAACCCTGGCAAcc                                                                                                            >  1:110324/1‑238 (MQ=255)
                                                                                                                                 gcgcGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCCTC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGAGGTTTTTGGCATCGTTACGGCCCATGTTTGACAGGTTGAAATTTCTGTcccacaccgggcaaagcg                                                                                                          >  2:1212091/1‑223 (MQ=255)
                                                                                                                                 gcgcGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCg                                                                                                           >  1:1005619/1‑238 (MQ=255)
                                                                                                                                      ttAAGGCGGTTGGTGGGGAAATCCGTAAGCATGCGCGCTTCCGCGCGGATCAACGCCAGCGCGTCCTCCTTGCCTTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACGTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCTCTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGcc                                                                                                      <  1:479705/238‑1 (MQ=255)
                                                                                                                                               ttGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTTAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCa                                                                                                                                                                                     >  2:796162/1‑150 (MQ=255)
                                                                                                                                               ttGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCa                                                                                                                                                                                     <  1:796162/150‑1 (MQ=255)
                                                                                                                                                   gggTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATCCGGCCACTGTTTGACAGGGTGAATTTACTTTTCGAACAGCGGCAACCGGAGCCAAAGGAaaaaaccg                                                                                        >  2:136581/1‑235 (MQ=255)
                                                                                                                                                    ggTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACCAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGc                                                                                        <  1:734833/238‑1 (MQ=255)
                                                                                                                                                        aaaTCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCCACTGTTTACAAGGTTGAATTTACTGTCCGGACCGGGGCAACCACCGCCAAAAGTAAAAcacaacccg                                                                                   >  2:551631/1‑231 (MQ=255)
                                                                                                                                                                       ggCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCAt                                                                                                                                                                >  2:394309/1‑147 (MQ=255)
                                                                                                                                                                       ggCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCAt                                                                                                                                                                <  1:394309/147‑1 (MQ=255)
                                                                                                                                                                              cggggctgtgacaCGCCAGCGCTTCTGCTTTGCATCCCCACAAC‑AGCTCGGCCGGATACTCCATC‑AACAGGCCTAGCATTTTGAGGATCTGCATTAGTCGCCCTCCGCTTTG‑GCGCGCCCTTCGGTGATGTGGATGGCACCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATcgc                                                             <  2:179780/227‑1 (MQ=255)
                                                                                                                                                                               gcggcgGATCAACGCCAGGCGGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACCCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATcgcc                                                            <  1:1030423/239‑1 (MQ=255)
                                                                                                                                                                                     gtccacccccgccgctacctcctTGCATCCCCCAACC‑AGCTCGTCCGGACACCCCATC‑ACCGGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCAt                                                      <  2:238222/222‑1 (MQ=255)
                                                                                                                                                                                             ccAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGAt                                              >  1:501860/1‑239 (MQ=255)
                                                                                                                                                                                                gcgcGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGCTggg                                           >  1:1108044/1‑238 (MQ=255)
                                                                                                                                                                                                 cgcgTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGCACAGGCTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCACAGGTAAAACCACCACCGGTGCGTTCCTCGAAGGCATCCCCCCCCATTTCCCGATGGcg                                          >  2:390327/1‑238 (MQ=255)
                                                                                                                                                                                                 cgcgTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTTTCCGAACCCTGGCAACCGTCGCCAAAGGTAAAACCCCAGCCGTTGCGTTCCTCGCAGGCATCCCCCCCCATTTCCCCATGGcg                                          >  2:1297464/1‑238 (MQ=255)
                                                                                                                                                                                                 cgcgTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGCGGCAACCGTCGCCAAAGGTAAAACAGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCCCCATTTCCCGATg                                             >  1:421702/1‑236 (MQ=255)
                                                                                                                                                                                                    gctcccctTGCTTTCCCTCAAC‑AGTTCTCTCGGTTCTCCCATC‑AACAGGCCGTTCACTTTGAGGATCTGAATTATTCGCCCTCCGCTTTG‑TCGCGCTCTTCGTTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGt                                       <  2:667781/234‑1 (MQ=255)
                                                                                                                                                                                                    gTCCTCTTTGACTTCCCCCACC‑GGCTCGGCGGAATATTCCATA‑AACGGGCGGATCACTTTGAGGGTCGGCTTTATTCGCCCTCCGTTTTG‑GCCCGGACTTCGGGGATGGTGATGGCATCGATACGGCTACGGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCAAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGt                                       <  2:1088530/239‑1 (MQ=255)
                                                                                                                                                                                                    gTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGt                                       <  1:63428/239‑1 (MQ=255)
                                                                                                                                                                                                     tcctccTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTCCTGTTGAACAGGTTGAATTTACTGTCCGAACCCTGGCAACCGTCGCCCAAGGTAAAACCCGAGCCGTTGCGTTCTGCGCAGGCATGGCCCGCCATTTCCCGATGGCTgggc                                      >  2:949181/1‑237 (MQ=255)
                                                                                                                                                                                                     tcctccTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATGGCCCGCCATTTCCCGATGGCTGTTc                                      >  1:68225/1‑239 (MQ=255)
                                                                                                                                                                                                     tcctccTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGCCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCCATGGCTGTTc                                      >  1:556019/1‑239 (MQ=255)
                                                                                                                                                                                                                  ccccCAAC‑AGCTCGTCCGGATTCTCCATC‑AACAGGCCGATCCCTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAAc                         <  1:28316/239‑1 (MQ=255)
                                                                                                                                                                                                                  cccatgac‑aGCTCGTCCGGATTCCCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAAc                         <  1:485322/233‑1 (MQ=255)
                                                                                                                                                                                                                  cccACAAC‑AGTTCTCCCGGATCCCCCATC‑AACAGGCCGTGCACTTTGAGGATCTGCATTATTCGCCCTCCGTTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAAc                         <  1:622831/239‑1 (MQ=255)
                                                                                                                                                                                                                  cccACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTTTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACaaaaa                         >  1:345238/1‑238 (MQ=255)
                                                                                                                                                                                                                  cccACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTTTGCGAAGGCATCCCCCGCCATTTCCCGATGGCTGGGCGGGATAACaaaaa                         >  2:965844/1‑238 (MQ=255)
                                                                                                                                                                                                                   ccccacacctcccGTCCGGATACTCCAAC‑AACAGGCCGATCTCTTTGAGGATTGGCATTATCCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAAc                         <  2:867683/227‑1 (MQ=255)
                                                                                                                                                                                                                         c‑aGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCCAAGGTAAAACCGCCGCCGTTGCGTTCTGCGAAGGCATCCCCCGCCTTTTCCCGATGGCCGGCCGGGGAAACaaaagcaaattc                  >  2:894107/1‑231 (MQ=255)
                                                                                                                                                                                                                             cTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGTTAACAAACCGAACTTCATa               >  1:176094/1‑239 (MQ=255)
                                                                                                                                                                                                                             cTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGACCTTctaa               >  1:538979/1‑236 (MQ=255)
                                                                                                                                                                                                                              tCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCCATGGCTGGTCGGGATAACAAAACGATCTTCATAg              >  1:120819/1‑239 (MQ=255)
                                                                                                                                                                                                                               cGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCCGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTCCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGGTACCAAAACGACCTTCATCGt             >  1:429128/1‑239 (MQ=255)
                                                                                                                                                                                                                                  ccGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTg           >  1:993995/1‑238 (MQ=255)
                                                                                                                                                                                                                                  ccGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGATGGTCGGGATAACAAAACGCTCCTCATAGTTgg          >  1:1286144/1‑239 (MQ=255)
                                                                                                                                                                                                                                          tCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCATCCGTTGCGTTCTGCGAAGGCATCGCCCGCCCTTTCCCGATGGCTGGTCGGGCTAACAAACCGATCTTCATACTTGGCAAAgggt  >  1:764352/1‑237 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                
GCCAGCAGGTCCACCATTGCCTGGCCGCGATC‑GCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGAC‑GTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAAC‑AGCTCGTCCGGATACTCCATC‑AACAGGCCGATCACTTTGAGGATCTGCATTATTCGCCCTCCGCTTTG‑TCGCGCACTTCGGTGATGTTGATGGCATCGATACGGCTACTGTTGAACAGGTTGAATTTACTGTCCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCG  >  NC_000913/1537044‑1537513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A