Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,274,856 G→T 13.9% T62K (ACA→AAA)  yjcB ← putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,274,8560GT13.9% 265.7 / 2.3 102T62K (ACA→AAA) yjcBputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (31/54);  new base T (15/2);  total (46/56)
Fisher's exact test for biased strand distribution p-value = 9.83e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

GGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTT  >  NC_000913/4274633‑4275087
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           
ggACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTTCTATTCTTCTTCTTTTTCATCACCCACCCCATTTTGTGTCCGACAGCGGTGATGTTCGCCCTCTTGA‑TTCCaaaaaaa                                                                                                                                                                                                                              >  2:796434/1‑233 (MQ=255)
      tgatgatgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCCCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCCGAAAGCGGGGATTTCCGCCCTTGTGT‑TTCCACC‑CAAACCCaat                                                                                                                                                                                                                       >  1:1152391/1‑236 (MQ=255)
        atgatgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTACTTCATCTCTTCTCTCTTCCACCACTCACAGGTGTGGCAAGTATCGGGGATTTCCGCCCGTTTGC‑TGCCCAG‑CAACCCCCTTgtg                                                                                                                                                                                                                    >  1:637245/1‑237 (MQ=255)
              aCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTTTCATCACCGAACCGATGTTTTGCCAGATAGCGGGGATGTCCGCCATTGTGA‑TGCCAAG‑CAAACCCCTTGCGAACCaa                                                                                                                                                                                                              >  1:852504/1‑239 (MQ=255)
                   ccGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGGGTTTGTTTTATTTTTTCTATACTCACCCACTCCGTATTTGGCCCGATAGCGGGGGTGTCCCCCCTTTTGG‑TGCCAAGACAAACCCTTTGAGaaaaaaagac                                                                                                                                                                                                          >  2:934543/1‑231 (MQ=255)
                         tGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAaac                                                                                                                                                                                                  <  1:796434/239‑1 (MQ=255)
                                tCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGGGTCTGTATCATCTCTTCTATAATCACCCCACCGCAGTTTTGCCAGTAAACGGGGTATTCCCACCTTTTGA‑TGCAAAG‑AAACCCCAATGTGAAACaaaaaacgggaaaaaaaccc                                                                                                                                                                                            >  2:72182/1‑221 (MQ=255)
                                    tAAAACAATAGTTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGGAACAACGCCCAGAc                                                                                                                                                                                       <  1:1012058/239‑1 (MQ=255)
                                            tAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGCAGTTGTGCCAGAAAGAGGGGATGTCCGCCCTTGTGA‑TGGCAAG‑CAAACCCCATGTGAACCAACAAGC‑GGGAACAACACCCAAAcccccgcgg                                                                                                                                                                               >  1:1135920/1‑232 (MQ=255)
                                               cggaaatgtgtataagagaaaggatTAAACCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGAt                                                                                                                                                                             <  1:1128365/216‑1 (MQ=255)
                                                    atTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTGTGATTCATCTCTTCTATCATCACCGACTCGAAGTTTTGCCAGAAAGCGGTGATGTTCGCCATCTTGA‑TTCCAAG‑CAAACCCCTTCGTAAACCACAGGA‑GGGAAAAACACCCCGCACCCAGCTGATCAcccc                                                                                                                                                                       >  1:872996/1‑237 (MQ=255)
                                                        ttttAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTTGTTCATCTCTTTTATCATCACCCACTCCATGTTGTGTCAGGTAGCGGTGGTGTTCCCCCTTTGGA‑TCGCAAG‑CAACCCCATTTCGAACCCACAAGC‑CGGCACAAAGCCCACAACCCAGCCGAACACCCCaaa                                                                                                                                                                    >  2:223670/1‑237 (MQ=255)
                                                        ttttAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTTATTTATCTCTTCTATCCTCCCCGACTCCATGTTGTGCCACATCGCGGTGATGTCTCCCCTTGTGA‑TGCCCAG‑CAACACCCTTGCCAACCAACAGTC‑GGAAAAAACGCCCCCACACCGCCTGATCACCgacag                                                                                                                                                                    >  1:835996/1‑234 (MQ=255)
                                                        ttttAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTTTCATCACCGACTCCATGTTGTGCCACATAAGTGTTATGTCATCCCTTTTGA‑TGCCAAC‑CAACCCCATTTCCTACCAAAACGC‑CGAGAAAACGtccccacactttctgacaaccaccac                                                                                                                                                                    >  2:973342/1‑215 (MQ=255)
                                                        ttttAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGc                                                                                                                                                                   <  1:521164/239‑1 (MQ=255)
                                                        ttttAAGGTGGATGATTAAAGACTCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTACCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGc                                                                                                                                                                   <  1:896980/239‑1 (MQ=255)
                                                         tttAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGCTGCTGATTCATCTCTTTTATCCTCAACGACCCCGTGTTTGGGCAGATATGGGTGCTGTCCGCCCTTTTGA‑TTGCCAC‑CAACCCCCTTCCCAACCAACAGACGGGAAAAAACCCCCacccacccctggctacccgcaac                                                                                                                                                                    >  2:592898/1‑216 (MQ=255)
                                                          ttAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTTATCTCCTCCATTCACACCGCATCGGTGTTGTGCCACATAACGGGGATGTTCCCCATTGTGA‑TTGCCAG‑CAACCCCATTTCGAACCAAAAGGC‑GGCAACAAACCCCCGAccccccccggtcaatcgcaaccc                                                                                                                                                                 >  1:624555/1‑218 (MQ=255)
                                                          ttAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCCAATAGCGGGGATGTCCGCCCTTTTTG‑TGCCCAG‑CAAACCCAATTCGAAACAAAACGC‑GGGAAAAAAACCCAAAcccccgccaaacaacccaaaccc                                                                                                                                                                 >  2:424349/1‑218 (MQ=255)
                                                            aagggtgtttattaaacgattgcccagccaAAAAAAAGAAGCCTCGTTTATGGGGGCTCTCACGTTCCGGGTAAAAACCATTGGCCATTGGGGCGGTGTCTGATCCATCTCTTCTATCATCCCCGACTCGATGTTGTGCCAGATGGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGa                                                                                                                                                               <  2:872996/216‑1 (MQ=255)
                                                            aaGGGGGATGATTAAGGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTAGGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGa                                                                                                                                                               <  1:800348/239‑1 (MQ=255)
                                                             aGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTTTGGTTTATTTCTTCTATCATCACCGACTCGCTGTTTTGCCAGATAGCGGGGATGTTCTCCCTTGTGT‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGGAACAACGCACAGAACgccgctcaccccccccaccccccc                                                                                                                                                              >  2:934868/1‑221 (MQ=255)
                                                             aGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAAATAGCGGGGATGGCCGCCATTTGGA‑TTCCAAG‑CAAACCCACTGGGGACCCACAAGC‑GGAAAAAACGCCCAGAACGCCCCCGATCACCGCAAAcccccc                                                                                                                                                              >  1:1174994/1‑236 (MQ=255)
                                                                  gatgatTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTTATTCATCTCTTCTATCATCACCAACTCGAGTTTTTGGCAGATAGCAGTGATGTCCTCCCTTTTGA‑TGGCCAG‑CAACACCCTTTCGAACCAAAAAGC‑GGAAAAAACACCCAGACACCCGCTGGTGACCGCAACGCCGAccaaaa                                                                                                                                                         >  1:976102/1‑234 (MQ=255)
                                                                  gatgatTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATTTCTTCTCTCTACACCCACTCGATGTTTTGCCAAATAACGGTGATTGACGCCATTTTGAGTTCAACG‑CAA‑CCCATTGTGCACCAAAAGGC‑GGGACAAACGCCCCGACCGCCGCTGATCCCCCCAAGCCCGCCATaa                                                                                                                                                          >  1:109625/1‑238 (MQ=255)
                                                                  gatgatTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTTTGCCAGATAACGGTGATTTTCGCCCTTGTGA‑TGCCAAG‑CAACCCCAATTCGAACCAAAAGGC‑GGGAACAAACCCCAGACCGCTGCTGATcccgtctcttttacacatc                                                                                                                                                          >  1:515721/1‑220 (MQ=255)
                                                                  gatgatTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGAATCATCTCTTCTATCATTACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCCTTGTGA‑ATCCAAG‑CAAACCCCTTTCGGACCAAAAGGC‑GGAAACAAAGCCCCAACCCCTGCTGCAGACCCCAAGccccgcctaat                                                                                                                                                         >  1:137579/1‑231 (MQ=255)
                                                                  gatgatTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCGGGTTCATCTCTTCTATCCTCACCGAATCGATGGTGTGCCCGATAGCGGTGATGTCCCCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGACCCAAAAGGC‑GGAAACAACCCCCCGACCGCCCCTGACCACCCCCAGCCCGCCCTaa                                                                                                                                                          >  1:750834/1‑238 (MQ=255)
                                                                  gatgatTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGGGTCTGATTCCTCTTTTCTATCATCACCGACTCGATGGTGGTCCCGATAGCGGTTGTGTTCGCCCATGTGA‑TGTCAAT‑CAAACCCCTTTCTAGACAACCCGC‑GGGAAGAACGCCCCAACCGCCGCCGGACACCGCCAGCCCGGCATaaa                                                                                                                                                         >  1:775209/1‑238 (MQ=255)
                                                                  gatgatTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGAGTCTGATTCATCTCTTCTATCATCACCGCCCCGATGTTTTTCCCGATAGGGGGGATGTTCGCCATTTTGA‑TGCCCAC‑CAAACCCCTTTCGGACCAACAAGG‑GGGAAAAAAACCCCCACCGCGCCTGCTCACCGCAAGCCCCACaaaaa                                                                                                                                                         >  2:903384/1‑238 (MQ=255)
                                                                      atTAAACCATCTGACAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGa                                                                                                                                                     <  1:667058/239‑1 (MQ=255)
                                                                       ttAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATTCCTTCTTTCATTACCGACTCCTAGTTTTGCCAGAAATCGGGATTGTCCGCCCTTTGTA‑TGCCCCA‑CAACCCCACTTCGAAACAACAAGC‑GGACAAAACGCCCCAAACCCCGCCGATCAcccccaaccccccacaaacaaac                                                                                                                                                    >  2:24762/1‑218 (MQ=255)
                                                                            tcATCTGCCAGCCATAAAAAAGAAGCCCCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCCTTTCTATCATCACCGACTCGATGTTGGGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACcgacg                                                                                                                                               <  1:521659/238‑1 (MQ=255)
                                                                              atgtGCCAGACAAAAAAAATAAGCCTCCGTTATGGAGTCTTCTACGTATCAGGTCAAAATCATGGGCCATGGTGGCGGGGTCTGATTCATCCCTTCTACCCACCCCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACcgacg                                                                                                                                               <  1:650232/234‑1 (MQ=255)
                                                                                cTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGGGTCTGAATCACCTCTTCTATCATCACCCACTCGATGTTGTGCCAGATAGCGGGGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACCCCCCCACCGCCGCTGCTCACCGCCAGCCCGACATAAACAGAACGACGcaaa                                                                                                                                           >  1:127117/1‑239 (MQ=255)
                                                                                        taTAAAAAAAACGCCCCGGTTTGGGGGGTTTCACCGTATCGTGGCAAAACTATGGGCCAGTGTGGGGGTGTCTGAGTCATCTCTTCCATCATCTCCGACCCGATGTGGTGCCAGATAGGGGTGATGGCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCGGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGAt                                                                                                                                      <  2:267988/235‑1 (MQ=255)
                                                                                          tAAAAAAGAACCCTCCTTTTGGGAGGCTTCTACGTATCAGTCAAAAATCATTGGCCTTTGTGGCGGTGTCTGATTCACTCCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGa                                                                                                                                 <  2:1135920/239‑1 (MQ=255)
                                                                                               aggtgtccTCCGTTGGGGGGGTTTCTACTTTACGGGCAAAAACCATTGGCCATTGTGGTGGTGTCGGATTGATCTCTTCTATCATCACCGACTCGATGTTCGGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGtgtg                                                                                                                            <  2:775209/233‑1 (MQ=255)
                                                                                                        ccGTTAGGGAGGCTTCTACGTATCAGGCCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCACCTCTTCTAGCATTACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGa                                                                                                                   <  1:658388/239‑1 (MQ=255)
                                                                                                                   gCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCCTTGCGGACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCCGACCGCCGAAAAAGGATGTTTCGTGTGCTGGGCAGAAAaatccataa                                                                                                        >  1:903233/1‑234 (MQ=255)
                                                                                                                   gCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTa                                                                                                         <  1:1107632/238‑1 (MQ=255)
                                                                                                                     ttttACGTTTCAGGGCAAAACTCTTGGCCATTGGGGCGGTTGCTGAGTCATCTCTTCTAACATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACg                                                                                                       <  1:484359/235‑1 (MQ=255)
                                                                                                                     ttCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCCCCCGCCCGATGTTGTGGCAGAAAGCGGGGATGTCCGCCCTTTTGG‑TGCCAAA‑CAACCCCCTTGCGAACCAACAGGC‑GGAAACAACGCCCCGACCGCCGCTGATCACCGCCAGCCCGGCAAAATCAAACCCCCCAAAACCGAGGTTTAGTGGGGTGGGCAaaaaaaaaaaatac                                                                                                       >  2:611761/1‑228 (MQ=255)
                                                                                                                     ttCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCGGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACg                                                                                                       <  1:223670/238‑1 (MQ=255)
                                                                                                                      tCTACTTAACAGGTCAAAATCATGGGCCATTGTGGCGGTGTTTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACgg                                                                                                      <  1:850992/238‑1 (MQ=255)
                                                                                                                                      aaaTCATGGGACATGGGGGCGGTGTCTGATTCATTTCTTCTATCATCACCGACCCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAg                                                                                     <  1:934543/239‑1 (MQ=255)
                                                                                                                                            ttGGCCATTGTGGCGGTGTCTGATTCCTCTCTTCTATCATCACCGAATCGATTGTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCgaaga                                                                               <  1:663873/239‑1 (MQ=255)
                                                                                                                                             tgtgtaaaagagactggttgtaTTCATCTCTTCTATCAACACCGACTCGATGTTGGGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCgaaga                                                                               <  1:600663/217‑1 (MQ=255)
                                                                                                                                                 gtttGGGGGGGTGTTTTTTTCTTTTCTTCTATCATCACCGTCCCGATTTTGTGCATGATGGCGGTGATGTCCGCCATGGTGA‑TGCCAAG‑CAACCCCATGGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AAc                                                                         <  2:109625/237‑1 (MQ=255)
                                                                                                                                                 cATTGGGGCGGTTTCTGATTCTTCCCTTCCATCATCACCGACCCGATGTTGTGCCAGATAGCGGTGATGCCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AAc                                                                         <  1:1376474/239‑1 (MQ=255)
                                                                                                                                                   ttgttgCGGTGTTTTATTCTTCTTTTCTTACACCACCGACTCGATTTTGTGCCAGATAGGGGTGATGTCCGCCATTGGGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCa                                                                       <  2:1152391/239‑1 (MQ=255)
                                                                                                                                                   ttGTGGGGGTGTCTGATTCATCTCTTCTATCAGCCCCGACTCGGTGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCa                                                                       <  1:426145/239‑1 (MQ=255)
                                                                                                                                                         cgggttCTGATGCATCTCTTCAAACATCCCCGACCCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCa                                                                  <  1:358236/233‑1 (MQ=255)
                                                                                                                                                         cGGTTTCTGATTCATCCCTTTAATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCa                                                                  <  1:934868/238‑1 (MQ=255)
                                                                                                                                                             gttgtattcactctttctATCCTCACCGAGTCGATGTTGTGCCAGATGGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAgtggt                                                             <  1:1352626/225‑1 (MQ=255)
                                                                                                                                                                tGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGAAATAATAAGACAGACGAAAACGGATGTTGCGTGTGCTGGCCAGAAACATAATTACGGCACTACGAAGTTGCCAGCGACGAGA‑AACCACGCCAGTGGTGAg                                                          >  1:252039/1‑239 (MQ=255)
                                                                                                                                                                  aTTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAGG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGGGCTGGCCGGAACACTCATTACGGCCCTAAAAAATTGCCACGGAAGAAA‑aacaacccacagggggaggg                                                        >  2:513351/1‑225 (MQ=255)
                                                                                                                                                                   ttCATCTTTTTTATCACCCCCGACCCGATGTTGGGCCAGAGAGGGGTGATGCCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGCCCGCTGCTGATCCCCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGt                                                       <  2:1174994/239‑1 (MQ=255)
                                                                                                                                                                      aTCTCTTTTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGAGGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGc                                                    <  1:842958/239‑1 (MQ=255)
                                                                                                                                                                        tctttctACCATCCCCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCa                                                  <  1:155859/236‑1 (MQ=255)
                                                                                                                                                                        ctctTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACcgac                                                                                                                                                <  1:1251158/146‑1 (MQ=255)
                                                                                                                                                                        ctctTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACcgac                                                                                                                                                >  2:1251158/1‑146 (MQ=255)
                                                                                                                                                                        ctctTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCCCTAAGAAGTTGCCAGCGAGGAAG‑AAACACGCCAGTGGAGGGGGTAGc                                                    >  1:946715/1‑237 (MQ=255)
                                                                                                                                                                        ctctTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCCCGCCAGTGGGGGGGGGAGCCa                                                  >  2:1258929/1‑239 (MQ=255)
                                                                                                                                                                        ctctTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGAGTGCTGGCCAGAAACATCATTACGGCACTAGGAAGTTGCCAGCGAAGAAG‑AACCACGcccatggctgttccttat                                                   >  1:49170/1‑222 (MQ=255)
                                                                                                                                                                           ttctATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCTAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACCTAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCCCTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGGGGTGAGGGAGGCCTTCa                                               >  1:262227/1‑239 (MQ=255)
                                                                                                                                                                                  acccccGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAAtt                                        <  1:592898/235‑1 (MQ=255)
                                                                                                                                                                                     cccGACTGGTTGTTGGGCCAGATAGCGGGAATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCct                                     <  1:769787/238‑1 (MQ=255)
                                                                                                                                                                                     aCCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTTAGGGTGCTGGCCAGAAACATCATTACGGCCCTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCCGTGGTGAGGGTAGCCCTCAAACAATTcc                                      >  1:746726/1‑238 (MQ=255)
                                                                                                                                                                                     aCCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCgaag                                                                                >  2:848504/1‑197 (MQ=255)
                                                                                                                                                                                     aCCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCgaag                                                                                <  1:848504/197‑1 (MQ=255)
                                                                                                                                                                                     aCCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGGGTGCTGGCCCGAAACATCATTACGGCACTCAGAAGTTGCCCGCGAAGAAA‑AACCACGCCAGTGGTTAGGGAAGCCATCAAACAATTcc                                      >  2:850202/1‑238 (MQ=255)
                                                                                                                                                                                          cTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTTCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGATTGTGCTGGCCAGAAACATCATTACGTCACTACGAAGGTGCCAGCGAAGACG‑AACCACGCCAGTGGGGGAGGTAACCATTAAAAAATTcccactaa                                >  2:1323814/1‑233 (MQ=255)
                                                                                                                                                                                          cTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGAAGCCATCAAACAATTCCTCTGaa                                >  1:592702/1‑239 (MQ=255)
                                                                                                                                                                                                  tttccgagttAGCGTTGATGTCCCCCCTTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTGGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCg                        <  2:592702/230‑1 (MQ=255)
                                                                                                                                                                                                  tgtgCCAGATAGGGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCg                        <  1:114696/239‑1 (MQ=255)
                                                                                                                                                                                                  tgtgCCAAGTAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCg                        <  1:1138457/239‑1 (MQ=255)
                                                                                                                                                                                                  tgtgCCAAATAGCGTTGATGTCCCCCATTGGGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCg                        <  1:989027/239‑1 (MQ=255)
                                                                                                                                                                                                  tgggCCATATAGGGGTGATGTCCGCCATTGGCA‑TGCCAAG‑CACCCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATACACGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCg                        <  1:1296827/236‑1 (MQ=255)
                                                                                                                                                                                                  tgggCCAGATAGGGGGGATGCCCGCCATTGTGA‑TGCCAAG‑CAACCCCACTGCCAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCg                        <  1:195280/236‑1 (MQ=255)
                                                                                                                                                                                                  tgggCCAGATAGCGGGGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCg                        <  1:24762/236‑1 (MQ=255)
                                                                                                                                                                                                  tgggCCAAATAGCGGTGATGGCCGCCATTGGGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCg                        <  1:424349/236‑1 (MQ=255)
                                                                                                                                                                                                  tggccagatatggcggggattCCGCCATTGTGA‑TGCCAAG‑CCCCCCCATTGCTAACCAACAGGC‑GGAAACAACGCGCAGACCGGTGCTGATCGCCGTAAGCCCGACATAATCAGCCCGACGAAAACGGATGTTGAGTGTGCTGTCCAGAAACATCAGTACGGCACTAAGAAGTTGCCTGCTAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCg                        <  2:354576/219‑1 (MQ=255)
                                                                                                                                                                                                  tggccaaGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCg                        <  1:927691/233‑1 (MQ=255)
                                                                                                                                                                                                  tgagcaaGATGGCGGGGAGGTCCGCACGTGTGA‑CGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCTCGGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTGGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCg                        <  1:717776/233‑1 (MQ=255)
                                                                                                                                                                                                  gtgcccgttgtgcgttatgttCCGCCATTTTGT‑GTCAAAG‑CAACCCCTTTGGGAACAAACAGGC‑GAAACCAAGCCCCAGACCGCTGCTGAACACCGCAAGCCCGAAATAAACAGACCGACGAAAACGGAGGTTGAGTGTGCTGGCCAAAAACATCATTACGGGACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCg                        <  2:80017/219‑1 (MQ=255)
                                                                                                                                                                                                  aaggcagtgtAGCGGGGAGGTCCCCCTTTTGGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCCCCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCg                        <  2:1252902/230‑1 (MQ=255)
                                                                                                                                                                                                    tgCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCCCTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGGGAGGGGAGCCATCAAACAATTCCTCTGAAAAAAGCCGaa                      >  2:238895/1‑238 (MQ=255)
                                                                                                                                                                                                    tgCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGGGGGTAGCCATCAAACAATTCCTCTGAAAAAAGCCGa                       >  1:897257/1‑238 (MQ=255)
                                                                                                                                                                                                    tgCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGGGAGGGTAGCCATCAAACAACTCCTCTGAAAACAGcccaa                      >  2:181558/1‑236 (MQ=255)
                                                                                                                                                                                                    ggccaaatggggggtaTGCCCGCCATTGTGG‑TGCCAAG‑CACCCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGGAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGAt                      <  2:503508/224‑1 (MQ=255)
                                                                                                                                                                                                        aggatgCGGTGATGGCCCCCATTGTGA‑TGCCAAG‑CAACCCCATGGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGcc                   <  2:903233/233‑1 (MQ=255)
                                                                                                                                                                                                            ggCGGGGATGGCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGa              <  1:1258929/238‑1 (MQ=255)
                                                                                                                                                                                                            aGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGa              <  1:72182/239‑1 (MQ=255)
                                                                                                                                                                                                                   aTGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGcgcg       >  1:964244/1‑239 (MQ=255)
                                                                                                                                                                                                                      tCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGc                                                                                                                                                                   >  2:1076953/1‑81 (MQ=255)
                                                                                                                                                                                                                      tCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGc                                                                                                                                                                   <  1:1076953/81‑1 (MQ=255)
                                                                                                                                                                                                                      tCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGACAAGAGCCGATGCCCTGGa              >  1:1252902/1‑229 (MQ=255)
                                                                                                                                                                                                                       ccGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCgcggcg    <  1:611761/238‑1 (MQ=255)
                                                                                                                                                                                                                        cGCCATTGTGA‑TGCCAAA‑GAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCCCAAGCCCGACATAATCAGACCGACGAAAACGGAAGTTTGGTGTGCTGGCCAGAAAAAACATTACGGCACAAAAAAAGTGCCAGCGAGAAAGAAACCAC‑CCAGGGGGGGGGGGAGCCCTCAAACAAATCCCCTGCAAAGAGCCCAAGCCCCGGaacagggggggcg  >  2:1107998/1‑227 (MQ=255)
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           
GGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGA‑TGCCAAG‑CAACCCCATTGCGAACCAACAGGC‑GGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAG‑AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTT  >  NC_000913/4274633‑4275087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A