Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F17 I0 R1	155	84.1	1797446	90.9%	1633878	230.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,536,735	A→C	15.0%	V192G (GTG→GGG)	narW ←	nitrate reductase 2 (NRZ), delta subunit (assembly subunit)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,536,735	0	A	C	15.0%	185.5 / 5.3	78	V192G (GTG→GGG)	narW	nitrate reductase 2 (NRZ), delta subunit (assembly subunit)
Reads supporting (aligned to +/- strand): ref base A (42/20); new base C (3/12); total (45/33)
Fisher's exact test for biased strand distribution p-value = 1.11e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGGAGGCGCGCCAGGTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATA  >  NC_000913/1536499‑1536973

gaggagGCGCGCCAGGTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGCCACACGCCGTGGCGTTATCTTCATTAAA‑CTTCA‑cc                                                                                                                                                                                                                                              >  2:300985/1‑239 (MQ=255)

     ggtccgcCAGGTGTACTGCCCGTAGTCGTAGAGCAGCCAGCTGCCGAGGAAAAACCCCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGtt                                                                                                                                                                                                                                          <  2:482283/234‑1 (MQ=255)

     ggCGCGCCAAGTGTCTCGCCCGTAGGCGTAGCCCCGACAGCCGGCGAGGAAAAACACCAGCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCCCCTCCCGCCCTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGtt                                                                                                                                                                                                                                          <  1:355738/238‑1 (MQ=255)

     ggCGCCACAGGTGTACTGCCCGTAGTTGTAGCGGCGCCAGGTGCCGAGGAAAAACCCCGTCGCAAAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCACCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGtt                                                                                                                                                                                                                                          <  1:625843/238‑1 (MQ=255)

       ccggcaaggggttcgtcccGGAGTCGTAGGCCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATAACGTAAAAAAAGACGTTCAGATACTGAAGCATTCCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTcc                                                                                                                                                                                                                                        <  1:105794/222‑1 (MQ=255)

           ccAGGTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGAAATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCCGCGAGCTGTCACACCCCGTGGGGTTATCTTCAAACAA‑CTTCC‑CCTGTTCCTCtt                                                                                                                                                                                                                                    >  1:585875/1‑238 (MQ=255)

                      gCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTAC‑CCTGTTCCTCTTCCCAAACTGCa                                                                                                                                                                                                                         >  2:798223/1‑238 (MQ=255)

                      gCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CCTCA‑CCTGTTCCTCTTCCCACACTGCAt                                                                                                                                                                                                                        >  2:114106/1‑239 (MQ=255)

                        cccgttgtgTAGCCCAGCCAGCTGGCAAGGAAAACCCCCCTCGCACAAATGTACGGGTATATATCGGAAAAAAAGCCGGTCAGATCCTGAATCATTTCCCTCCTCCCGCACTGATGTCGACATATCGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTACGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCa                                                                                                                                                                                                                      <  1:444387/231‑1 (MQ=255)

                                 tAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGCCATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACCCTGCATCAAGCGCCTgg                                                                                                                                                                                                             >  2:297144/1‑239 (MQ=255)

                                 tAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGCTATTGATTAAGCGGCGAGCTGTCACACGCCCTGGCGTTATCTTCAATAAA‑CTTCA‑CCTTTTCCTCTTCCCACCCCCCCTCAAGCCCCTgg                                                                                                                                                                                                             >  1:56443/1‑239 (MQ=255)

                                 tAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACCCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAgccgcggg                                                                                                                                                                                                             >  1:433688/1‑233 (MQ=255)

                                        gccagcTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGGGCTGTCACACGCCGGGGGGTTATTTTCAATAAA‑CTTAA‑CCTGTTCCTCTTCCCACCCTGCATCAAGACCCCGGCggggg                                                                                                                                                                                                       >  1:768700/1‑236 (MQ=255)

                                        gccagcTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGGGCCGTTATCTTAAATAAA‑CTTAA‑CCTGTTCCTCTTCCCCCACGCAATCAAGCGCCTgcgggggt                                                                                                                                                                                                       >  1:863045/1‑231 (MQ=255)

                                              tGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGCCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTGC‑CCTGTTCCCTCTCCCCCACTGCATCAACCCCCTGCCGGGGGGCAAcgc                                                                                                                                                                                                >  1:649360/1‑239 (MQ=255)

                                                      aaaaaCACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATTCTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCtt                                                                                                                                                                                          <  2:357845/237‑1 (MQ=255)

                                                       aaacccccGTTGCAAAAATGTGCGGGGAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCC‑CCTGTGCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATAGCGCTCTTCg                                                                                                                                                                                        <  1:96499/232‑1 (MQ=255)

                                                               gTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCCCCCCCTTCCCTGttt                                                                                                                                                                                  >  1:146848/1‑236 (MQ=255)

                                                               gTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGGGTCATCGGCATCTTCCCTGTTTACt                                                                                                                                                                               >  2:747577/1‑239 (MQ=255)

                                                                       aaTGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGGCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTtgt                                                                                                                                                                       <  2:146848/239‑1 (MQ=255)

                                                                           ttcgggtaatatgcGTAAAAAAAGACTTTGAGAAAGGGGATGATTGCCCACCACCCGAGAGGATGGCGACATATTGCGGCGCGACAGCATGGCTAAAGCGTCTCTGATAATGAGTAAGCGGCGAGCTGGCACACGCCGAGGCGTTATCTTCAATAAA‑CTTCG‑CCGGTACCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGaca                                                                                                                                                                   <  1:300985/226‑1 (MQ=255)

                                                                            aCGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGacac                                                                                                                                                                  <  2:650605/239‑1 (MQ=255)

                                                                             cGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTTCATCAAGCGCCTGCCGGGTGTCATCCCCCCCTTCCCGGTTTCCTTTTTTtgggca                                                                                                                                                                    >  1:143335/1‑232 (MQ=255)

                                                                                 tAGATATCGTAAAAAAATGCGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGGCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTc                                                                                                                                                              <  2:863045/238‑1 (MQ=255)

                                                                                 tAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTc                                                                                                                                                              <  2:56443/238‑1 (MQ=255)

                                                                                 tAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTc                                                                                                                                                              <  2:768700/238‑1 (MQ=255)

                                                                                    atatCGTAAAAAAAGCGCTTCAGATACTGAATCATTTCCCTCCCCCCGCATGGATGTCGAAATATTGCGGCGCGACATCCGGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACt                                                                                                                                                           <  1:482517/238‑1 (MQ=255)

                                                                                    atatCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAACCCCTGGCGGGGGTCATCCCCCCTTTCCCCGTTTTCTtttttttggcaccgttcactt                                                                                                                                                          >  1:97106/1‑224 (MQ=255)

                                                                                     tatCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCCCGCTCTTCGCTGTTTACTTGTTTTTTGAACCTGTCCCTTg                                                                                                                                                         >  2:248314/1‑239 (MQ=255)

                                                                                          tAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTTGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTACCTTGAAAgg                                                                                                                                                    >  2:637698/1‑239 (MQ=255)

                                                                                          tAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCATCTTCCCACACTGCATCAAGCGCCTGCCGGGGGGCATCGCGCTCTTCGCGGTTTCCTTGTTTGTTGGTACTGTCACTTGCAAgg                                                                                                                                                    >  2:121509/1‑239 (MQ=255)

                                                                                                              cTGAATCATTTCCCGCCTCCCGACCTGAGGTCGACATTTTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCC‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgcag                                                                                                                                <  2:711108/239‑1 (MQ=255)

                                                                                                               tGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCAAACTGCATCAATCGCCTGGCGGGTGTCATCGCGCACTTCACTGTTTACTTGTTTTTTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTGgcccc                                                                                                                               >  2:526802/1‑236 (MQ=255)

                                                                                                               cgAATCTTTTCCCCCCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGGGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTG‑TTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgcagca                                                                                                                              <  2:612538/238‑1 (MQ=255)

                                                                                                                gAACCATTTCCCCCCCCCCGCCCTGATGCCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCTCTGTTATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgcagca                                                                                                                              <  2:756382/239‑1 (MQ=255)

                                                                                                                 aaTCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGcagcag                                                                                                                             >  2:53205/1‑239 (MQ=255)

                                                                                                                 aaTCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTAACTTGAAAGGGAGCTTCCCGCCAGTTGcagcag                                                                                                                             >  2:167487/1‑239 (MQ=255)

                                                                                                                   tCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGGGCTTCCCGCCAGTTGcagcagg                                                                                                                            >  2:217432/1‑237 (MQ=255)

                                                                                                                   ccttttCCCCCCCCCCGCTCTGTTTTCTATATTTTGCGGCCTACCATCCTGGCTAACGGGTGTGTGATATTGATTAAGCGGCGAGCTGTCCACCGCCGGGGCTTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCaga                                                                                                                            <  1:297144/235‑1 (MQ=255)

                                                                                                                     aTTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGGGTCATCGCGCTCTTCGCTGTTTACTTGTTTTTTGACCCCGCACCTTGAAAGGGGACTTCCCCCCCGtttccacaaggat                                                                                                                         >  1:711108/1‑228 (MQ=255)

                                                                                                                      tttCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTTTGACCCTTTCACTTGAAAGGGAGCTTCCCGCCAGTTgcaccaaaacac                                                                                                                        >  1:144688/1‑233 (MQ=255)

                                                                                                                                       tgtgttcgaaattgtgcggccgtaCATCTTGGCTAAAGCGTGCATGATTTGGTTTAAGGCGCGTGTCGCCACACGCCGTGGCTTTATCTTCAATAAA‑CTTCC‑CCTTTTCCTCTTCCCCCACTGCATCAAGCGCCTGGGGGGTGTCATCGCGCTCTTCGCCGTTTACTTGTTTTGGGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCAc                                                                                                       <  1:433053/216‑1 (MQ=255)

                                                                                                                                         aTGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTTTGCCACTGTCACTTGAAAGAGAGCTTCCCGCCAGTTGCAGCAGACAGTCAAACACAGCGTACCACgg                                                                                                     >  2:97498/1‑239 (MQ=255)

                                                                                                                                         aTGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTTACCCTTTCCCTTGCAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTCCCACgg                                                                                                     >  2:433053/1‑239 (MQ=255)

                                                                                                                                           gTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTTCAGCAGAGCATCAAACAACGCGTACCACGGt                                                                                                    >  2:310036/1‑238 (MQ=255)

                                                                                                                                                   atgtgggccCGAAATCCGGGCTAAAGCGTGGCTGATTTTGATTAGGCGGCCAGCTTTCCCAGTCCGGGGCGTTATCTTCAATAAA‑CTTCC‑CGTGTTCCTCTACCCTCCCGTCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGGGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgt                                                                                           <  1:637698/234‑1 (MQ=255)

                                                                                                                                                   atTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgt                                                                                           <  2:610299/239‑1 (MQ=255)

                                                                                                                                                   atTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGGGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGTGGCCTCGCgt                                                                                           >  2:493599/1‑239 (MQ=255)

                                                                                                                                                     tGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCCGTTGCAGCAGAGCATCAAACAAAGCGTACCACGGGGCCTCGCgttg                                                                                         >  2:44138/1‑239 (MQ=255)

                                                                                                                                                                                cGCTGATATTGCTTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCCCTCTTAGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTGCCCGCCAGTTGCACCAGAGCAGCAAAAAAACCGTCACAAGGGGCCCCCCGGTGTTTTAAGCGCCCGCCAAAAAGGGCggg                                                              >  1:403962/1‑237 (MQ=255)

                                                                                                                                                                                cGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGCAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACCCGTAACACGGTGCCTCGCGTTGTTTTAAGCGACCCCCAACCAGGGCggg                                                              >  1:725150/1‑237 (MQ=255)

                                                                                                                                                                                cGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGGGCCTCGCGTTGTTTTAAGGGACGGCCAAGCAGGGCGAg                                                              >  2:203447/1‑239 (MQ=255)

                                                                                                                                                                                    gATTTTGATTAAGCGGCGAGCTGTCCCGCGCCGGGGCGTTAACCTCAATAAA‑CTTCC‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGAt                                                           <  2:433688/238‑1 (MQ=255)

                                                                                                                                                                                       atTGATTAAGCGGCGAGCTGTCCCACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTCCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGgc                                                       <  2:97106/239‑1 (MQ=255)

                                                                                                                                                                                        ttGATTAAGCGGCGAGCTGTCACACGCCGTGGCTTTATCTTCAATAAA‑CTTCC‑CCTGTTCCTCTTCCCACCCTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGgcg                                                      <  2:725150/239‑1 (MQ=255)

                                                                                                                                                                                        gtgtttAACCGGGGTGGTGTCCCACGCCGTGGGGTTATTTTCAATAAA‑CTTCC‑CCTGTTCCGCTTCCCACCCTGCTTCAAGCGCCTGGGGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGGGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGgcg                                                      <  1:53205/235‑1 (MQ=255)

                                                                                                                                                                                               aGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAAAGCGTACCCCGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGCCGCAACCtt                                               >  2:514087/1‑239 (MQ=255)

                                                                                                                                                                                                   ggcgccTGTCCCACCCCGTGGGGTT‑TCCTCAAAAAACCTTCA‑CCTGTTCCCTTTCCCCCACTGCAGCAAGCGCAGGGCGGGTGTCATCGCGGTCTTCGTTGTTTTCTTGTTTTGTGACACTGTCGCTTGAAAGGGAGCGTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGc                                           <  1:805193/234‑1 (MQ=255)

                                                                                                                                                                                                    cgggCTGCCCCACGACGTGGCGTTATCTTAAAAAAA‑CTTCC‑CCTTTTCCTCTCCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACCCTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGc                                           <  2:138537/235‑1 (MQ=255)

                                                                                                                                                                                                    cgAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCCGTTGCAGCAGAGCATCAAACAACGCGTCCCACGGGGCCTCGCGTTGTTTTAAGCGACCGCCAACAAGGGCGGGGGTCGGCGGAAAATTGTGCa                                          >  1:745577/1‑239 (MQ=255)

                                                                                                                                                                                                     gAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACAGTGCCTCGCGTTGTTTTAAGCGACCGCCACGCAGGGCGAGGGGCGGCGCAAAATGGGGCaa                                         >  1:479343/1‑239 (MQ=255)

                                                                                                                                                                                                     gAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACCCGTACCACGGTGCCTCGCCTTGTTTTAACCGACCGCCAAACCGGGCGAAGAACGGCGCAAAATTGAGCa                                          >  1:351182/1‑238 (MQ=255)

                                                                                                                                                                                                                   cggtgtgTTTTCTGCAATAAC‑CTCCC‑CCTGTCCCTCTTCCTCCACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTTTGTTGTGACACTGTCCCTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTGCCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCt                           <  1:273419/233‑1 (MQ=255)

                                                                                                                                                                                                                   cGTGGCTTTTTCTTGAA‑ATA‑CTTTACCCTGCTCCTCTTCCCCCCCTGCATCAAGCCACTGAGGGGTGCGCTGGGTCTTTTCGCGGTTTACTTGTTTTGTGACACTGACACTTGAAAGGGAGTGTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCt                           <  1:741210/239‑1 (MQ=255)

                                                                                                                                                                                                                   cGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCCGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCg                              >  2:464219/1‑236 (MQ=255)

                                                                                                                                                                                                                   cGTGGCGTTATCCTCAATAAA‑CTTCC‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTTTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCt                           <  2:21462/239‑1 (MQ=255)

                                                                                                                                                                                                                     ttgCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTAATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGGGAGGATCGGGGCAACATTGAGCAATCCTTCTTTCCCCTgg                         >  1:756366/3‑238 (MQ=255)

                                                                                                                                                                                                                     tGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTTCAGCAGAGCATCAAACAACGCGTCCCACGGTTCCTCGCGTTGTTTTAAGCGACCGCCCAACAGGGCTGGGATCGGCGCAACCTTGGGCAATCCTTTTTTcccccac                         >  1:806246/1‑236 (MQ=255)

                                                                                                                                                                                                                     tGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCCCGTTGTTTTAAGCGACCGCCAAGCAGGGCGGGGATCGGGGCAACATTTGGCAAACCTTCTTTTGCCTg                          >  1:378232/1‑238 (MQ=255)

                                                                                                                                                                                                                             tCTTCAATAAA‑CTTAC‑CCTGTTCCTCTTCCCACACTTCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCTGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAgg                 <  2:585875/239‑1 (MQ=255)

                                                                                                                                                                                                                               ttCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGAATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGGCGGGATCGGCCCAACATTGAGCAATCCTTCTTTCGCCTGATCggcaggca               >  1:115121/1‑239 (MQ=255)

                                                                                                                                                                                                                                  aaTAAC‑CTTCC‑CCTGTTCCCTTTCCCACCCTGCATCAAGGGCCTGGCGGGTGGCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTa            <  1:479776/239‑1 (MQ=255)

                                                                                                                                                                                                                                  aaTAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGCGCTTCCCGCCAGTTGCAGCAGAGCATCACACAACGCGTACCACGGTGCCTCGCGTTGTTTTAATCGACCGTCAAGCAGGGAGAGGCTCGGCGCAACATTGAGCAATCCTCCTTCCGCCTGATCGCCCGGCAgca            >  1:602283/1‑237 (MQ=255)

                                                                                                                                                                                                                                  aaTAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTTAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGt             >  2:54737/1‑238 (MQ=255)

                                                                                                                                                                                                                                       a‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCCCGTTGTTTTAAGCGCCCGCCAAGCAGGGCGAGGATCGGCGCAACATTGGGCAATCCTTCTTTCGCCTTATCGTCAGGCAGGACGCtt       >  1:387922/1‑239 (MQ=255)

                                                                                                                                                                                                                                           tCA‑CCTTTTCCCCTTCCCACACTGAATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTaaa    <  2:144688/239‑1 (MQ=255)

                                                                                                                                                                                                                                            cA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAAt   >  2:479776/1‑239 (MQ=255)

                                                                                                                                                                                                                                            cA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGAACGCTTaaaa   >  1:494366/1‑238 (MQ=255)

                                                                                                                                                                                                                                             a‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCCACATTGAGCAATCCTTCTTTCCCCTGGTCGTCAGGCAGTAAGCTTaaaaa  >  1:746035/1‑237 (MQ=255)

GAGGAGGCGCGCCAGGTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATA  >  NC_000913/1536499‑1536973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A