Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F17 I0 R1 155 84.1 1797446 90.9% 1633878 230.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,154,491 T→C 20.2% L159P (CTC→CCC)  mdtA → multidrug efflux system, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,154,4910TC20.2% 121.5 / 4.3 72L159P (CTC→CCC) mdtAmultidrug efflux system, subunit A
Reads supporting (aligned to +/- strand):  ref base T (26/28);  new base C (9/7);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 7.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTACCGCCGCTAATACCGTTACGGTGCGCAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTT  >  NC_000913/2154259‑2154722
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        
aTTACCGCCGCTAATACCGTTACGGTGCGCAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCCCGttt                                                                                                                                                                                                                                     >  2:330767/1‑238 (MQ=255)
         gcTAATACCGTTACGGTGCGCAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTACCCAAAGCCCAAGGCCACCTGGCACAAGAT‑AAAGCCACGCTTGACAACGCCCGCCGGGACCTGGCGCCGTATCAAACACCGGCAAAAACCAATCCCGTTTCCCGCCCGGa                                                                                                                                                                                                                           >  1:237821/1‑239 (MQ=255)
                   ttACGGTGCGCAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCCCGCTTGCCCACGCCCGCCGTGACCTGGCGCGTTATCAACAAATGGCAAAAACCACACCCGTTTCCCGCCGGGAGCTGGATGc                                                                                                                                                                                                                  >  1:689588/1‑238 (MQ=255)
                           cgcAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTTTCAACAACTGGCAAAAACCAATACCGTTTCCCCCCAGGAGCTGGATGCCCAAAAGgc                                                                                                                                                                                                         >  1:292347/1‑239 (MQ=255)
                           cgcAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGgc                                                                                                                                                                                                         >  2:314236/1‑239 (MQ=255)
                             cAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCAAAAGCCCAGGGCCAACGGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGCCCTGGCGCGTTATGAACACACGGAACAACACAACCCCGGTCCCCGCCAGGAGCCGGAGGCCCAACaagggc                                                                                                                                                                                                       >  1:563080/1‑234 (MQ=255)
                                ccGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACACCTGGCAAAAACCAACCCCCTTTCCCGCCCGGGGCGGGATGCCCAACAGGCGCTgg                                                                                                                                                                                                    >  1:716453/1‑239 (MQ=255)
                                ccGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTgg                                                                                                                                                                                                    >  2:690556/1‑239 (MQ=255)
                                     tGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGTAGCCCAACAGGCGCTGGTCAGt                                                                                                                                                                                               >  2:247081/1‑239 (MQ=255)
                                        aCGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGCT‑AAAGCCACGCTTGCCAACGCCCGCCCTGACCTGGCGCGTTATCAAAAACTGGCAAAAACCAATCCCGTTTCCCGCCCGGGGCTGGATACCCAAAAggacatggacaaggaa                                                                                                                                                                                            >  1:858767/1‑225 (MQ=255)
                                                 gaatggttttatttttCCAGGAAGGCCGGCAGGAAAAGGAAGGTGTTTTACTGGAAAAAATT‑GCCCCAAGCCAGTTAAAAGTTGCATGAGCACAAGCCCGGGGCCACCGGGCAAAAGAT‑AAACCCCCGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGc                                                                                                                                                                                  <  1:314236/226‑1 (MQ=255)
                                                      gCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCATGCGCGTTATCAACAACTGGCAAAACACAATCTCGTTTCCCCCCCGGACATGGGTGCCCAACGGGCCGTGCTCACTG‑AAAcacacgggcacaa                                                                                                                                                                             >  1:121728/1‑227 (MQ=255)
                                                      gCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAACCCACGCTTGCCAACGCCCACCGTGCCCTGGCGCGTTATCAACAACTTGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACACGCGCTGGACAGAG‑AACCCGAAGGCAcc                                                                                                                                                                               >  2:159241/1‑237 (MQ=255)
                                                          tACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCATTG‑AAACCGAAGGCAACATTAAg                                                                                                                                                                         >  2:184469/1‑239 (MQ=255)
                                                           aCATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAg                                                                                                                                                                         >  2:863597/1‑238 (MQ=255)
                                                           aCATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCaa                                                                                                                                                                                                              >  2:757148/1‑202 (MQ=255)
                                                                 ccgagAAGGCCAGAGGGACAAAGCAGGCGTTTTACTGGCAAAAATT‑GCCCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCAAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCtgatg                                                                                                                                                                  <  2:226057/235‑1 (MQ=255)
                                                                 ccAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCCAAAACCAATCTCGTTTCCCGCCAGGAACTGGATGCCCAACAGCCGCTGGGCAGTG‑AACCCGAAGGCACCCTTTATGCtgaag                                                                                                                                                                  >  2:801966/1‑237 (MQ=255)
                                                                      aaGGCCGGCAGGTCAAGGCAGGCG‑TTTACCGGGAAAAATTGGACCCCAGCCAGTCCAAAGTTCAATTAGCACAAGCCCAGGGCCAACGGGCAAAAGAA‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGaag                                                                                                                                                               <  2:428537/237‑1 (MQ=255)
                                                                       gggCCAGGAGGTAAAAGCAGTCGTTTTACGGAGAGAATGG‑CACCCACCCCTGTTCAAAGTTGCATTAGCCAAACCCCGGGGCCACCGGGCAAAAGAA‑AAACCCACGTTTGCCACCGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGaagca                                                                                                                                                             <  1:516880/237‑1 (MQ=255)
                                                                       aGGCCAGCAGGTCAAAGCGGGGGATTACCGGGCAAAAATT‑GCCCCCAGCCAGTTAAAAGTTGAATTAGCACAAGCCCAGGGCCAACGGGCAAAAGAA‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGaagca                                                                                                                                                             <  2:716453/238‑1 (MQ=255)
                                                                        ggCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCCCTGGTCAGTG‑AAACCGAAGGCCCCATTAAAGCTGATGaagaaag                                                                                                                                                           >  1:193844/1‑239 (MQ=255)
                                                                         gCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCACGGTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGGG‑AAACCCAAGGCACCATTAAgagtgagaaagaaaac                                                                                                                                                          >  1:366414/1‑224 (MQ=255)
                                                                                   cAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAATCCACGCTTGCCAACGCCCGCCGTCACCTGGCGCGTTATCAACCACTGGCAAAAACCAATCTCGGTTCCCGCCAGTGGCCGGATGCCCACAAGCCCCTGGCCCGGG‑AAACACCAGACACCATTAAGGACAAAGaagcaagagattcaaagg                                                                                                                                                >  1:388806/1‑228 (MQ=255)
                                                                                           gCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGCAGGAACCATTAAGGCTGATGAAGCAACCGTTTCCTGCGCGCAGcct                                                                                                                                        >  1:158874/1‑237 (MQ=255)
                                                                                           gCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCCCGTTTCCCGCCCGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCCAAGGCCCACTTAGGGCTGCTGAAGCCACCGCTGCCAGGCCGCAGCt                                                                                                                                         >  1:55720/1‑238 (MQ=255)
                                                                                                                        cacgTTGAAATTTGCATTGGCAAAAGCCAGGGGCCAACGGGAAAAAGAT‑AAACCCAAGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCAc                                                                                                            <  2:767962/236‑1 (MQ=255)
                                                                                                                                      cATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACa                                                                                                                                                                                                            >  2:330064/1‑130 (MQ=255)
                                                                                                                                      cATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGCCTGGAGCCGGATTACCGCACCAGCCGAGGGTcgc                                                                                              >  2:391665/1‑239 (MQ=255)
                                                                                                                                        ttACCACCACCCCGGGCCCATCTGGAAAAAAAA‑AACCCCACCTTTGCCAACCCCCGCCGTGCCCTGGCGCGTTACCAACAACGGGCAAAACCCAATCTCGGTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGt                                                                                            <  1:242013/239‑1 (MQ=255)
                                                                                                                                        tgagcccaggccaggcgCCACCTGGAAAAAGAT‑AAAGCCACGCTTGCCAACCCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGt                                                                                            <  2:292347/223‑1 (MQ=255)
                                                                                                                                           gCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGACGAAGCAAGCGTTGCCAGCCCGAAGCTCCAACTCGACCGGAGCCGGCTTACCGCACCAGCCCATggctgccttg                                                                                          >  2:425021/1‑230 (MQ=255)
                                                                                                                                             ccaACCCCCGGGCCCATGGGGAAAAGATAAAACCCCCGCTTCCCACCCCCCGCCGTGACCGGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGt                                                                                        <  1:690556/238‑1 (MQ=255)
                                                                                                                                                aGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGACGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGGCGCACCCCAACGGGAGCCGGATTACCGCCCCAGCCGATGGTCGCGTTgggccaa                                                                                    >  1:499818/1‑234 (MQ=255)
                                                                                                                                                 gCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGGCAGCGCGCAGCTGCAACTCGACTGGGTCCGGATTAACGGCCCAGCCGACggggcggttgggcccaa                                                                                   >  1:73127/1‑224 (MQ=255)
                                                                                                                                                   ccAGGCCAAACTGGAAAAAAAA‑AAAGCCACGGTTGCCAAGCCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAg                                                                                  <  2:700888/238‑1 (MQ=255)
                                                                                                                                                      gggCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAgg                                                                              >  2:169768/1‑239 (MQ=255)
                                                                                                                                                            aCTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGTGCTCAAGCAGGTTGATg                                                                        >  2:752410/1‑239 (MQ=255)
                                                                                                                                                            aCTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATg                                                                        >  2:231589/1‑239 (MQ=255)
                                                                                                                                                            aCTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCAGCAACTCGACTGGAGCCGGATAACCGCACCAGCCGATGGTCGCGTTGGGCTCAAGCAGGTTGATg                                                                        >  2:880907/1‑239 (MQ=255)
                                                                                                                                                            aCTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGCTGCCAGCGCGCCGCTGCAACCCGACTGGGGCCGGGTTACCGCCCCAGGCGATGGGCCCGTGGGGCTCACGCCGGTTGagt                                                                        >  1:66664/1‑237 (MQ=255)
                                                                                                                                                                        taaaaCCCACTTTTGCAAACGCCCGCCGTGACCTGGCGCTTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCaa                                                             <  2:657492/237‑1 (MQ=255)
                                                                                                                                                                        ctacccccccttttccccccccccgccGGGACCGGGCGGTTTAACAAAAACGGGAAAAAACCAATCTCGTTCCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCaa                                                             <  1:169768/218‑1 (MQ=255)
                                                                                                                                                                        ataaaccccccttgcccAACGCCCGCCGTGACCTGGCGCGTTACCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCaa                                                             <  2:158874/224‑1 (MQ=255)
                                                                                                                                                                        acaaccgcctctttGCCACCGCCCGCCGCGACCTGGGGCTTTAGCAACACTGGGCAAACACCAATCTCTGTCCACGCCAGGAGTGGGATGCCCAACAGGCGCTTGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAAATCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCaa                                                             <  1:656662/227‑1 (MQ=255)
                                                                                                                                                                           aaGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAGTCAGGTTGATGTTGGTAACCAAAt                                                           >  2:252960/1‑238 (MQ=255)
                                                                                                                                                                                  gCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGACGGTGGTAACCAAAACTCCAatg                                                   >  2:185925/1‑239 (MQ=255)
                                                                                                                                                                                      gCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGACCCGGATTACCGCACCAGTCGACGGCCGCGTCGGTCCCAAGCAGGTTGATGTTGGTAACCAAATCACCAGGGGGGa                                               >  1:700823/1‑239 (MQ=255)
                                                                                                                                                                                       ccccccccccccgggcacTGGAGCGTTACCAACAATGGGCAAAAACCAACCTCGGTTCCCGCCAGGGGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCAGTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGAt                                              <  1:250878/222‑1 (MQ=255)
                                                                                                                                                                                       ccactgcccgccGTGACCTTGGGGTTTAACAAAAACCGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCTCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGAt                                              <  1:180080/234‑1 (MQ=255)
                                                                                                                                                                                        cAACGCCCGCCGGGACCTGGCGGGTTAGCAACAACGGGAAAAAACCAATCGCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATa                                             <  2:827476/239‑1 (MQ=255)
                                                                                                                                                                                         cccccccgccGTGTCCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATac                                            <  1:391665/235‑1 (MQ=255)
                                                                                                                                                                                             ccccccTTGCCCTGGCGCTTTTTTAACAACTGGAAAAACCCAATCTCGTTTCCCGCCAGGAGCTGGATGACCAACAGGCGCTGGTCAGTGAAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATaccac                                         <  1:330767/239‑1 (MQ=255)
                                                                                                                                                                                                    tgcccTGGCGCGTTATCAACAACCGGCAAAAACCATTCTCGTTTCCCGACAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCg                                 <  2:402075/236‑1 (MQ=255)
                                                                                                                                                                                                     gcccTGGAGCTTTATCCACAACTGGCAAAAACCAAACTCGTTGCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGAAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCgt                                <  2:699507/237‑1 (MQ=255)
                                                                                                                                                                                                      aCCGGGCGCGTTAACAAAAACTGGCAAAAACCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCgt                                <  2:699506/238‑1 (MQ=255)
                                                                                                                                                                                                        cgggCGCGGTATAAACAACTGGCAAAACCCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCgtggt                             <  2:143650/237‑1 (MQ=255)
                                                                                                                                                                                                         tGGGGCGTTACCAACACCTGGCAAAACCCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCgtggtg                            <  2:182002/239‑1 (MQ=255)
                                                                                                                                                                                                         tGGCGGGTTACCAACAACTGGCAAAAGCCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCgtggtg                            <  2:138913/239‑1 (MQ=255)
                                                                                                                                                                                                          ggggcgTTATCAACAACTGGCAAAAACCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGa                           <  2:22090/236‑1 (MQ=255)
                                                                                                                                                                                                          ggCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAACCGTTGCCAGCGCGCAGCTGCACCTCGCCTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCCCAAGCAGGTTGATGTTGGTAACCAAAACTCAAGTGGTGATAAAACCGGGATCggggtgc                           >  1:574553/1‑238 (MQ=255)
                                                                                                                                                                                                            cgcgTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGAt                          <  2:66664/238‑1 (MQ=255)
                                                                                                                                                                                                               gTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCAcc                      <  2:168757/239‑1 (MQ=255)
                                                                                                                                                                                                                ttaacaaacacTGGAAAAACCCAAGCTCTTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCAcc                      <  1:247081/229‑1 (MQ=255)
                                                                                                                                                                                                                ttATCAACAACTGGGAAAAACCAATCCGGTTTCCCGCCAGGAGATGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGAAGAAGCAAGCGTTGCCAGCGCGCATCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATTGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCTcc                      <  1:159241/238‑1 (MQ=255)
                                                                                                                                                                                                                ttATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGTTCGTGGGGATCAccc                     >  2:207520/1‑239 (MQ=255)
                                                                                                                                                                                                                         acgtgaaaaaaCCAATCTCTTTTCCCCCCAGGGGCTGGATGCCCAACAGGGGCTGGTCGGGG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATc            <  1:8451/233‑1 (MQ=255)
                                                                                                                                                                                                                            ggCAAAAACCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCAACGGGCGCTGGTCAGTG‑AAACCGAAGGCCCCCTTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTa         <  2:121728/239‑1 (MQ=255)
                                                                                                                                                                                                                              cAAAACCCTATCCCGTTTCCCGCCAGGGCGTGGACGCCACACAGGCGCTGGTTAGTG‑AAACCGGAGGCACCATTAAGGCTGAAGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTAt        <  1:131415/238‑1 (MQ=255)
                                                                                                                                                                                                                              cAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTAt        <  2:689588/238‑1 (MQ=255)
                                                                                                                                                                                                                              cAAAAACCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTAt        <  2:578393/238‑1 (MQ=255)
                                                                                                                                                                                                                                   aCCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGAttt  <  2:574588/239‑1 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        
ATTACCGCCGCTAATACCGTTACGGTGCGCAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATT‑GACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGAT‑AAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTG‑AAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTT  >  NC_000913/2154259‑2154722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A