Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F17 I0 R1 155 84.1 1797446 90.9% 1633878 230.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,530,175 T→A 13.1% I24F (ATC→TTC)  sgcC ← putative PTS system EIIC permease component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,530,1750TA13.1% 262.2 / 3.9 101I24F (ATC→TTC) sgcCputative PTS system EIIC permease component
Reads supporting (aligned to +/- strand):  ref base T (57/29);  new base A (3/12);  total (60/41)
Fisher's exact test for biased strand distribution p-value = 1.20e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 101 total aligned reads displayed.

AATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGTTACAGTTAAGAGGGATCTCA  >  NC_000913/4529948‑4530412
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                
aagaaCTGGGGTAATCAAGGCCCCAATGGCTGTCGCATAACCCACGCCGGAGTCAGGGCCTGCGCCAACGGCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCGCTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCa                                                                                                                                                                                                                                       <  2:538509/236‑1 (MQ=255)
  tAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCAAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑g                                                                                                                                                                                                                                    <  2:759608/239‑1 (MQ=255)
    acggggaaaaacagtgcgcAAATGGCTTGCGCAAAACCACCGCCGGAGGTAGGCCCGACGCCAAAGTAAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACGGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GAt                                                                                                                                                                                                                                  <  1:146529/224‑1 (MQ=255)
     cTGGGATAAACATGGCGCCAATGGGTGGCGCATAACCAACGGCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATc                                                                                                                                                                                                                                 <  2:718732/239‑1 (MQ=255)
       gggataatcttgtgcctataggCTGGCGCATAACCACCCCGCGAGGCAGGGCCCGCGCCAACGTCAAAGAAATGCAGGGCTAGGCCGAAACGCACAATCATCACTTTGATAGGGGGACTGAGGCTGGCGATCGCCATGACGATCGCCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCat                                                                                                                                                                                                                               <  1:281955/219‑1 (MQ=255)
          aTAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCACCCAAGGGATGCGGCAGAAAAAGCCaaaacaacaaaagag                                                                                                                                                                                                                             >  1:367444/1‑226 (MQ=255)
             aTCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATgatgat                                                                                                                                                                                                                         >  2:872471/1‑239 (MQ=255)
              tCATGGCGCCAATGGGTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAC‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATa                                                                                                                                                                                                                        <  2:229807/239‑1 (MQ=255)
                  ggCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAA‑CGCCAAAACCAAT‑CCCACCCGTTACCCCCGCCCTAATCGCCTCCAGCCCGGGGATGCGGAAACAAAAGCCAACAAC‑AATACTGATGAAAGGGa                                                                                                                                                                                                                    >  1:465265/1‑239 (MQ=255)
                   gcgcCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATTCC‑GATCATGAATATAGGGAc                                                                                                                                                                                                                   >  2:287135/1‑239 (MQ=255)
                        aaTGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATACTGATGAAAGGGACGaaaa                                                                                                                                                                                                              >  2:421200/1‑239 (MQ=255)
                        aaTGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGCCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATGGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCATTCAC‑GATCATGATGATAGGGACGcaa                                                                                                                                                                                                               >  2:849362/1‑238 (MQ=255)
                         aTGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCGTAC‑CGCCAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGGAAGTAAGGCCAATCCA‑GATCCTGGTGAAAGGGAGGaaaaa                                                                                                                                                                                                             >  1:86769/1‑238 (MQ=255)
                         aTGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGAGCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCATTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATTGGGAAGATAAGGCCAAACAC‑Gaccccaacaaaagggacaaaaaa                                                                                                                                                                                                             >  1:851165/1‑217 (MQ=255)
                               ggcGCATACCCAACGCCGGAGGCAGGGCCTGGGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTAc                                                                                                                                                                                                        <  2:131164/236‑1 (MQ=255)
                                tCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAGCGCTCAATCATCCCTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCACT‑CCCAACCGTTACCCCTGCCTTATTCGCCCGCAGCCCGGGGATCCGGAACATAAGGACAACCAC‑GAACCAGAAGAAAGGGACGAAAAAGGAAca                                                                                                                                                                                                       >  1:91695/1‑237 (MQ=255)
                                      aaCCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCACTCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGGTACCCCTGCCTTAATCGCCTGCAGCCCGGGGATGCGGACGATAAGGCCAACCAC‑CATCATGATGAAAGGGCCGaacagggaacaaccggag                                                                                                                                                                                                >  1:571462/1‑223 (MQ=255)
                                            cGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAAt                                                                                                                                                                                                                                         >  2:445902/1‑193 (MQ=255)
                                            cGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCCCCTGCAACCCGGGGATTCGGAAGCAAAAGCCAATCAC‑CATAATGATGATAGGGGCGAAAACGGTACAGCCGAGGGAC‑Agg                                                                                                                                                                                         >  1:672100/1‑239 (MQ=255)
                                            cGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGAATGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAAt                                                                                                                                                                                                                                         <  1:445902/193‑1 (MQ=255)
                                                 gAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTACCATAA‑CGACAAAACCAAT‑CCCAACCGTTACCACTGCTTTAATCGCACACAGCTCGGGGATCCGGAAGATAAGGCAAATACC‑GCTCCTGAGGTAAGGGCGGCAAACGTCAccgccacaaagaagggtgaa                                                                                                                                                                                     >  1:33754/1‑224 (MQ=255)
                                                       gggCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGCTGATAGGGACGAAAACGGTACCCCCGAGAGAC‑AGGATGTAATCaaa                                                                                                                                                                              >  2:814990/1‑239 (MQ=255)
                                                              cgcCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAAt                                                                                                                                                                        >  2:802080/1‑238 (MQ=255)
                                                              cgcCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACACCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCCC‑GATCAGGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑CGGATGTAATGAAACATaag                                                                                                                                                                        >  2:658050/1‑237 (MQ=255)
                                                              ccccAACGGAAAAGCAAGGCAGGGTTGGGCCAAAACGCTAAACCATCACTTTGATGGGGGGACTGAGGCTGTCAATCGCCCTGACGATCCCCAGCCCCATAC‑CAAAAAAACAAAT‑CCAACCCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATAATGATGATAGGGACGAAAAGGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATg                                                                                                                                                                       <  1:872471/237‑1 (MQ=255)
                                                                        aaaGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGCTGATCGGGACGAAAACGAGACCGCCGGGGACC‑CGGATGTAATCAAACACAAAGCCCCCTTTTa                                                                                                                                                             >  1:493011/1‑239 (MQ=255)
                                                                               tGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCCC‑GACACTGATGATAGGGACGAAAACGGTACCCCCG‑GGGACAAGGTTGTAATCAAACAAAATGCCTCCTTTTTTTGAGtt                                                                                                                                                      >  1:873002/1‑239 (MQ=255)
                                                                                gCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑a                                                                                                                                                    >  2:75107/1‑239 (MQ=255)
                                                                                      cTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAA‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AAAaga                                                                                                                                               >  2:431584/1‑238 (MQ=255)
                                                                                              aaaCGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCCC‑GATCATGTGTATAGGGCCGAAAACGGTACCGCCGAGAGAC‑AGGATGGAAACAAACATAATGCCCCCCTTTTTTGAGTT‑AAAAAAGACTGGAtt                                                                                                                                      >  1:258435/1‑239 (MQ=255)
                                                                                              aaaCGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCGGCCTTAATCGCCTGCAGCCAGGGGATGCGGACGCTAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGGGAC‑CGGAGGTAATCAAAAATAATCCACCCTTTTATTAGGTT‑AAAAAGGCCTGGAtt                                                                                                                                      >  1:820182/1‑239 (MQ=255)
                                                                                                aCGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGGGTT‑AACAGAGCCTTGATttg                                                                                                                                    >  2:533502/1‑239 (MQ=255)
                                                                                                            caCTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATTGGGTCGAAAACGGTACCGCCGAGAGAC‑AGGAGTGAATCAAACACAATGCCTCCTTTTATTTGGTT‑AACAGAGCCTTGATTTGTTGTTTTAAcgc                                                                                                                        >  1:889863/1‑239 (MQ=255)
                                                                                                             aCTTTGATAGGGGAACTGAGGCAGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAAGGGATGCGGAAGATAAAGCCAATCAC‑GATCATGATGATAGGGACGAAAACGATACCGCCGAGCGAC‑AGGGTGTAAACAAACATAAACCCTCCTTTTTTTCAGTT‑AAAAAAGCCTTGTTTTGTTTTTTAAAcgct                                                                                                                       >  1:405417/1‑238 (MQ=255)
                                                                                                              ttttGATGGGGGGCCTGAGGCTGTCGATCGCTACACATACCCCCAGCCCCATAC‑CGAAAAACCCAAC‑CCCACCCTTTACCCCGTCCTTAATCGCCGGGCGCGAGGGGATGGGAAAGAAAAGGCAAATCAC‑GATCTTGATGATAGGGACAAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGcgt                                                                                                                      <  1:576696/238‑1 (MQ=255)
                                                                                                              cTTTGATGGGGGGAGTGGGGCTGTCGACCCCCATGACGATCCCCAGTCCTATAC‑CGAAAAAACAAAT‑CCCAACCGTTCCCCCTGCCTTAATCGCCTGCAGCAAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGcgt                                                                                                                      <  2:509038/239‑1 (MQ=255)
                                                                                                              cTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGTGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCATCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAAcgcg                                                                                                                       >  2:777353/1‑238 (MQ=255)
                                                                                                                gtGAAAGGGGGACGGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCGGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAATCGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGcgtcg                                                                                                                    <  2:672100/238‑1 (MQ=255)
                                                                                                                        gggATTGAGGTTGTCGATCGCAATGACGACCACCATCCCCAACC‑CGAAAAACCCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGGGGAAGATAAGGCAAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGa                                                                                                             <  2:367444/238‑1 (MQ=255)
                                                                                                                        gggACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGa                                                                                                             >  2:482346/1‑238 (MQ=255)
                                                                                                                         ggACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGAt                                                                                                            >  2:777237/1‑238 (MQ=255)
                                                                                                                         ggACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGAt                                                                                                            >  2:723593/1‑238 (MQ=255)
                                                                                                                         ggACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTCCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATACGGCCGAAAACGGTACCGCCGGGAGAC‑ACGCTGTATTCAAACATAATGCCCCCTTTTATTGCGTTAAACAG‑GCCTTCATTTGTTGTTTTAACGCGTCCTCCTTTAt                                                                                                            >  1:870925/1‑238 (MQ=255)
                                                                                                                             tGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCCGGGGATGCGGAAGATAAGGCCAATCCC‑GATCATGATGATAGGGGCGAAAACGGTACCGCCGGGCAAC‑AGGCTGTAATCAAAAATAATGCCTCCTTTTTTTTAGTT‑AACAGGGCCTTGGTTTGTTGTTTTAACGCGTCGCAGTTTATCCCtt                                                                                                       >  1:277499/1‑238 (MQ=255)
                                                                                                                             tGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCtg                                                                                                       >  2:450165/1‑239 (MQ=255)
                                                                                                                                 gctgcccagcGCCATGGCAATCACCATTCCCATAC‑CGACAAACCCAAT‑CCCAACCGTTCCCCCTGCCTAAACCGCCTGCAGCCAGGGGATGCGGAAAAAAAGG‑CAATCCC‑GATCATGAGTATAGGGACGAAACCGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAg                                                                                                   <  2:250551/229‑1 (MQ=255)
                                                                                                                                      cgcacGCCATGACGACCCCCAGGCCCATAC‑CGAAAAAACCAAT‑CCCAACCGTTCCCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCAGGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAggg                                                                                              <  2:621833/235‑1 (MQ=255)
                                                                                                                                        tccccccgacccaTCGCCAGACCAATGC‑CGACAAAACCAAC‑CCCACCCGTTCCCCTTCCTTTAACACCCGGCAGCCGGGGGATGCGGAATATGAGTCCAATCAC‑GATCATGTTGATAGGAAAGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGggcg                                                                                            <  1:658050/227‑1 (MQ=255)
                                                                                                                                           gCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCGGCTTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACTGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGgcggcg                                                                                         <  2:493011/239‑1 (MQ=255)
                                                                                                                                               tGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGGGCAGGGCGGGGCCAt                                                                                     >  2:375012/1‑239 (MQ=255)
                                                                                                                                                gccgtcacccAGTCCAATAC‑CCAAAAAACCAAT‑CCCAACCGTTCCCCCTGCCTTAATCGCTGGCAGCCAGGGGATGCGGAAGATAAGGCAAATCAC‑GATCATGATGATAGGGACGAAACCGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCAtt                                                                                    <  1:709410/231‑1 (MQ=255)
                                                                                                                                                  agATCACCAG‑CCCCTACACGAACAAACCAAT‑CCCAACCGTTACCCCGTCCTTAATCGCCTGCAGCCAGGGGAGGCGGAATATAAGGCAAATCAC‑GAACATGATTATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCAtt                                                                                    <  2:247540/236‑1 (MQ=255)
                                                                                                                                                  acatacccAGTCCCATAC‑CGAAAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCAGGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCAtt                                                                                    <  2:871915/231‑1 (MQ=255)
                                                                                                                                                     tCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGCTTTGTTGTTTTAACGCGTCGTCGTTGATCCCGTTGAGCAGGGCGGCGCCATTAAGCg                                                                               >  2:823309/1‑239 (MQ=255)
                                                                                                                                                          gtacccctcc‑cGACAAAAACGAT‑CCAAGCCGTTCCCCTGGTCTGAATCGGAAGCGGCAGGGGAAGGCGGAAGAAAAGGCCAATCCA‑GGTTAGGATGATAGGGCAGAAAAGGGGACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCGGTGAGCAGGGCGGCGCCATTAAGCGTAtgg                                                                          <  1:542813/230‑1 (MQ=255)
                                                                                                                                                           gTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGGGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGACGGGCGGCGCCTTTAAGCGTAggaa                                                                         >  2:774561/1‑237 (MQ=255)
                                                                                                                                                           gTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAACGGTAgggt                                                                         >  2:82851/1‑238 (MQ=255)
                                                                                                                                                           gTCCCATAC‑CAACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGGGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGTTCCCTGTGAGCAGGGCGCGCCCCTTAAGCCTGGGGa                                                                         >  2:704402/1‑239 (MQ=255)
                                                                                                                                                            tCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCTTCGTTGATCCCTGTGAGCAGGGCGGGGCCATAaagggaaggggg                                                                        >  1:373074/1‑237 (MQ=255)
                                                                                                                                                            tCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGAt                                                                        >  2:874825/1‑239 (MQ=255)
                                                                                                                                                            tCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAACGGTAGGGAt                                                                        >  2:654308/1‑239 (MQ=255)
                                                                                                                                                            ccccATCC‑CAAAAAACCCAAA‑CCCCACCGTTACCCCCGCCTTAATGCCCTGCAGCCGGGGTATGCGAAAGAAAGGGCCAACCCG‑GTTCAGGATGAGAGGGAAGAAACGGGTACCGCCGAGAGGC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGAt                                                                        <  1:874825/238‑1 (MQ=255)
                                                                                                                                                             cccATAC‑CCAAAAAACCAAC‑CCCAACCGTTCCCCCTCCCTTAATCGCCTGCAGCCAGGGGAGGCGGAAGATAAGGCCAATCAC‑GATAATGATGATGGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAggg                                                                          <  2:465265/236‑1 (MQ=255)
                                                                                                                                                                   c‑cGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACCGAGCCTTGATTTGTTGTTTTAACGCATCGGCGTTGATCCCTGTGCGCAGGGCGGGGCCATTAAACGTAGGGATggcaaaa                                                                 >  1:256578/1‑233 (MQ=255)
                                                                                                                                                                    acccaaaaaCCAATACCCACCCTATCCACCGGCCTTAATCGCCCGCGGCAGGGGGTAGGGGAAGAAAAGCCAAATCAC‑TAAAATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTa                                                                  <  1:753701/234‑1 (MQ=255)
                                                                                                                                                                       aCAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATc                                                               >  2:457613/1‑238 (MQ=255)
                                                                                                                                                                       aCAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATc                                                               >  2:550341/1‑238 (MQ=255)
                                                                                                                                                                       aCAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGACGTCGTTGATCCCTGGGAGCAGGGCGGCGCCATTAAGCGGAGGGAGGCCGGAATCa                                                              >  1:711752/1‑239 (MQ=255)
                                                                                                                                                                       aCAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCATCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTTGTCGTTGATCCCTTGGGGCAGGGGGGGGCCATTAAGGGAAGGGTGGCCGGaaac                                                               >  1:285603/1‑236 (MQ=255)
                                                                                                                                                                                       aCCGTTCCCCCTGATTTAATCGCCTGCAGCAAGGGGGTGCGGAAGATAAGGCAAATCAC‑GATCAGGATGATGGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATg                                               <  2:870925/239‑1 (MQ=255)
                                                                                                                                                                                       aCCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGGGCAGGGGGGCGCCCTTAAGCGTAGGGAGGCCGTAACCACTTTTGGTTCGCATg                                               >  1:2586/1‑239 (MQ=255)
                                                                                                                                                                                       aCCGTTACCCCGGCCTTAATCGCGTGCAGCCAGGGGATGCGGAAGATAAGGCAAATCAC‑GATCATGAAGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATg                                               <  2:523085/239‑1 (MQ=255)
                                                                                                                                                                                            tcccctcgctttAATCGCTGGCGGCCAGGGGATGGGAAAGATAAGGCCATTCCG‑GATATGGGTTATAGGAACGAAAACGGAACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGt                                          <  1:421200/229‑1 (MQ=255)
                                                                                                                                                                                            tcccccTGCCTAAATCGCCTGCAGCCGGGGGAGGCGGAAGAAAAGGCCAATCCC‑GATCATGATGATAGGGACAAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGt                                          <  2:86769/237‑1 (MQ=255)
                                                                                                                                                                                            tACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTGGATCCCTTTGTGCAGGGCGGCGCCATTAAGCGTGGGGAGGCCGAAATCCCTTTTGGTTCGCAGGGAGGt                                          >  1:523085/1‑239 (MQ=255)
                                                                                                                                                                                             aCCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCGTTGAGCAGGGCGGCGCCATTAACCGCAGGGATGCCGCAATCACTGTTGGttgccccgggggtt                                         >  1:658689/1‑227 (MQ=255)
                                                                                                                                                                                              ccccTGCCTTAATCGCCTGCGGCCAGGGGATGGGGAAGATAAGGCAAATCAC‑GATCAGGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTa                                        <  2:314340/239‑1 (MQ=255)
                                                                                                                                                                                                  tGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGCGCAGGGCGGAGCCATTAAGCGTAGGGATGCCGTAATCACTTTTGGTTGTCTGGGAGGttcaaaa                                    >  1:699521/1‑234 (MQ=255)
                                                                                                                                                                                                  gtccTTAAGCCCCTGCAGCCAGGGGAGGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAAt                                    <  2:873002/237‑1 (MQ=255)
                                                                                                                                                                                                  gcgcTTACTCCCCTGCTGCCGGGGGGTGCGGAAAAAAGGGCCAATCAC‑GATCATGATGATGGGGACGAAAGCGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATGTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAAt                                    <  1:631181/236‑1 (MQ=255)
                                                                                                                                                                                                   gCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTTATCCCTGTAGGCAGGGCGTCCCCGTTCAGGGAAGGGAGCCAGTAAACACTGATGGtcgccgaggggggtgaaaaa                                   >  1:16512/1‑220 (MQ=255)
                                                                                                                                                                                                   gCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCCCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTataata                                   >  2:136310/1‑239 (MQ=255)
                                                                                                                                                                                                   cccTTAAGCCCCGGCGGCCGGGGGAGGGGAAAGAAGAGGCCAACCAC‑GATCATTATGATGTGGAAAAAACCGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATa                                   <  1:777237/238‑1 (MQ=255)
                                                                                                                                                                                                    cctaaacccccaggaGCCGGGGGAAGGGGAAGAAAAGGCAAATCAA‑GAACATGATGATAGGGACGAAAAGGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATa                                   <  2:277499/224‑1 (MQ=255)
                                                                                                                                                                                                     ctaaaTCGCCTGCAGCCAGGGGATGCGGAAGAAAAGGCCAATCAC‑GATCATGATGATAGGGACAAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAAt                                                                <  2:537448/205‑1 (MQ=255)
                                                                                                                                                                                                         aTCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCCCTGTTGGTTCGCATGGAGGTTACAATAAGGtac                             >  2:631181/1‑237 (MQ=255)
                                                                                                                                                                                                         aTCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACGGTGGGTTCGCATGGGGGTTAAAAAAAGGTc                              >  1:314340/1‑238 (MQ=255)
                                                                                                                                                                                                          tCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GTTCATGAAGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCa                            <  2:285603/239‑1 (MQ=255)
                                                                                                                                                                                                              cTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTTAGCAGGGCGGCGCCATTAAGCGTAGGGAGGCCGTAATCACTGTTGGGTCCGATGGAGGTTACAAAAAGGGCCAAAcc                        >  1:580958/1‑239 (MQ=255)
                                                                                                                                                                                                              cTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGGGGCGCCATTAAGCGTAGGGATGCCGTAATCCCTGTTGGTTGGCGTGGAGGTTAAAAAAAGGCCCAAAcg                        >  1:437569/1‑238 (MQ=255)
                                                                                                                                                                                                               tGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGGGGTTACAATAAGGTCCATACCg                       >  2:270695/1‑239 (MQ=255)
                                                                                                                                                                                                                gctgCCAGGGTATTCGGAAGTTAAGGCCAA‑CACACATCATGTTGAGAGGGCACAAAATGGTACCGCCGAGATAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGt                      <  2:266568/236‑1 (MQ=255)
                                                                                                                                                                                                                 cccccAGGGGATGCGGAATATAAGGCCAAACAC‑GATCAGTGTGATAGGAACGAAAGCGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTATAATAAGGTCCATACCGtt                     <  1:136310/236‑1 (MQ=255)
                                                                                                                                                                                                                 caggcaggGGATGGGAAAAGTAGGGCAAACAAG‑GATTATGATGTGGGGGACGAAAGAGGTTCCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGt                      <  1:326092/238‑1 (MQ=255)
                                                                                                                                                                                                                 cagcagggggATGCGAAAGTTAAGACCAATCAC‑GATAATGTGGATAGGGACGAAAGCGTTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGt                      <  2:697912/232‑1 (MQ=255)
                                                                                                                                                                                                                   gcagggggAAGCGAAAGAAAAGGCCAATCAC‑GATCAGGATGAAAGGGACGAAACCGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGTTa                    <  2:91695/234‑1 (MQ=255)
                                                                                                                                                                                                                   gCCAGGGGATGGGGAAGAAAAGGCCAATCAC‑GATTAGGATGATAGGGACGAAAACGGTACCGGCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGTTAc                   <  2:580958/239‑1 (MQ=255)
                                                                                                                                                                                                                        gggATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACTCGTCGTCGTTGATCCCTGTGAGCGGGGCGGCGGCATTAAGCGTAGGGATGCCGTAGTCACCTTTGGTTCGCATGGAGGTTCAAATAAGGACCATACCGTAACaataa              >  1:484667/1‑235 (MQ=255)
                                                                                                                                                                                                                           atacGGAAGATAAGGCCAATCCC‑GATCATGATGATCGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTACTCAAACATAATGCCGCCTTTTATTGAGTT‑AACACAGCCTTGATTTGTTGTTTTAACGCGTCGCCGTTGATCCCTGTGAGCAGGGCGGAGCCATTAAGCGTAGGGATGCCGTAACCACTGTAGGTTCGCATGGCGGGTACAATAGGGCCCTAACAGTAACAGTTaaac           >  1:142381/4‑237 (MQ=255)
                                                                                                                                                                                                                                    aTAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATAcctgtctcttttacccatctccga  >  2:53278/1‑217 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                
AATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGTTACAGTTAAGAGGGATCTCA  >  NC_000913/4529948‑4530412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A