Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F9 I0 R1 71 67.1 1514203 90.7% 1373382 220.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,080,362 A→C 27.6% V77G (GTG→GGG)  yeeA ← putative transporter, FUSC family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,080,3620AC27.6% 56.1 / 2.0 44V77G (GTG→GGG) yeeAputative transporter, FUSC family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (23/7);  new base C (2/12);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.76e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCG‑GGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAAC‑CTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAG‑TTGCAGAGCGATAAGACCTAAAATCGAACCCAACAC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAG‑CAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGC‑GGTATACCCGGATTTCAAACGGGCTTAATGACTTAT  >  NC_000913/2080138‑2080581
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                              
agCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCG‑GGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAAC‑CTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTCACTCCAG‑TTCCAAAGCGATAAGACCTAAAATCGAACCCAACAC‑Cg                                                                                                                                                                                                                            >  2:448398/1‑228 (MQ=255)
     cggATCACATCGCCGCTGCGCCATAACGACGGATCAATTTACCCTGTCG‑GGGAACCCACAACAATTGCCAGCTTCACCCCAATCAATAAAC‑CTTGATACGGTTTCTTGCCCAGCGCCTGCCCACCGCAAAGGAACATGGCAGCCGCGCACCAGAGTAACATCAGCGGTAACAAGATTAACTCCAG‑TTCCAGAACACTAAGAACTAAAAACAAACACAAAAC‑CGTAccac                                                                                                                                                                                                                      >  2:175877/2‑227 (MQ=255)
                gccgcTTCGCCATAACGCCGTATCAATTTCACCTGTCG‑GGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAAC‑CTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAG‑TTGCAGAGCGATAAGACCTAAAATCGAACCCAACAC‑CGTACCGCCAAtcacctca                                                                                                                                                                                                           >  1:41087/1‑222 (MQ=255)
                    cTTCGCCCTAACGCCGTATCAATTTCACCTGTCG‑GCGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAAC‑CTTGATACGGTTTCGTGCCCAACGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGGACCAGGCTAACAACAACGGCAACAAGATTAACTCCGA‑CTGCATAGCGACAAGGCCTAAAACCACACCCAACAC‑AGAACCGCCAAAACGccccacgc                                                                                                                                                                                                       >  2:140728/1‑222 (MQ=255)
                     ttCGCCATAACGCCGTATCAATTTCACCTGTCG‑GGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAAC‑CTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCCGACTAACCTCAGCGGAAACGAGATTAACTCCAG‑TTGCAGAGCGATAAGACCTAAAATCGAACCCAACAC‑CGCACCCACAATACCCCCAAAGgc                                                                                                                                                                                                      >  2:442436/1‑229 (MQ=255)
                        cccATAGCGCCGTATCATGTTCCCCTGTCG‑GGGAACCCACAACAATGCCCAGCGACACCCCAATCAATAACC‑CTTGATACGTTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAG‑TGGCAGAGCGATAAGACCTAAAATCGAACCCAACAC‑CGTACCGCCAATACGCTCAAAGGcgcg                                                                                                                                                                                                   <  1:113329/228‑1 (MQ=255)
                             aaCCCCGTATCAATTCCACCTGTCG‑GGGAACCCAAAACAATTGCCGGCGTCCCCCCAATCAAAAAAC‑CTTGAGCCGTTTTCTCGCCGAGCGCCAGCAAGCCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACAACAGCGGTAACGAGATTACCTCCAG‑TTGAAGAGCGATAAGACCTAAAAACGAACCCAACAC‑CGTGCCGCCAATACGCTCAAAGGCGCGAGGGa                                                                                                                                                                                              <  2:254407/229‑1 (MQ=255)
                              tccccttATCAATTTCCCCTGTCG‑GGGAACCCACACAAATTCCCCGCGTCCCCCCAATAAAAAATC‑CTTGATGCGGTTTCTGCCCCAGCGCCGGCCAACCGCAAAGAAACATGGCCGCCGCGCACCAGACTAACATCAGCGGAAACGATATAACCGCACG‑TTGCAGAGCGATAGGCCCAAAAATCGAACCCAACCC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGAc                                                                                                                                                                                             <  2:41087/223‑1 (MQ=255)
                              acgccggtccattttCA‑CTGTCGCGGGAACCCCGAACCTTTGCCAGCTGCCCCCCATACAAAAAAC‑CTTGATCCGGTTCCTTGCCCAGCCCCAGCAAACCGCAAAGGAACACGGCCGCCGCGCTCCAGACTAACACCAGCGTTAACGACATTAACCCCAG‑TTGCAGAGCGATAAGCCCTAAAATCGAACCCAACCC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGAc                                                                                                                                                                                             <  2:391173/217‑1 (MQ=255)
                              acccccttccAATTTCACCTGTCG‑GGAACCCCACAACTATGGCCAGCGTCCCCACAAGAAAGACCCTCTTG‑CCCTGTTTCTTCCCCGAGTACAGCACACCGCAAAGGAACATGGCCGCCGCGACCCAAACTACCAACAGCGGTAACGCAATTACCTCCAG‑TGGCAGAGCGATAAGACCTAAAACCTAACCCTACCC‑CGTCCCGCCAATACGCTCAAAGGCGCGAGGGAc                                                                                                                                                                                             <  2:515196/220‑1 (MQ=255)
                                    tATCAATTTCACCTGTCG‑GGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAAC‑CTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAG‑TTGCAGCACGCTAAAACCTCACATCGAACCCAACCC‑CCTACCGCCAATCGCCTCAAAGGACCGAGGGCCAACGtc                                                                                                                                                                                       >  2:113329/1‑228 (MQ=255)
                                    tATCAATTTCACCTGTCG‑GGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAAC‑CTTGATACGGTTTCTTGCCAAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGAGGTAACGAGATTAACTCCAG‑TTGCAGCGCGATAAGACCTAAAATCGAACCAAACAC‑CGCACCGGCAATAGGCTCCAAGGCGCAAGGGACAAcact                                                                                                                                                                                       >  1:714860/1‑226 (MQ=255)
                                                  gTCG‑GGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAAC‑CTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAG‑TTGCAGAGCGATAAGACCTAAAATCGAACCCAAACC‑CGGACCGCCAAAACGCTCAAAGGCGCAGGGGACAACGTTACCCCAGacagaaa                                                                                                                                                                         >  2:32503/1‑223 (MQ=255)
                                                         gAACCCACAACAATTCCCAGCGCCCCCCCAAACAAAAACC‑CTCGATACGGTTTCTCGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACCCCAG‑TTGCAGAGCGATAAGACCTAAAATCGAACCCAACCC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGcc                                                                                                                                                                   <  1:169257/229‑1 (MQ=255)
                                                                caacaaTTGCAAGGCGCACCCCAAGCAATAACC‑CTGGATACGGTTGCTCGCCCGGCGCAAGCCAACCGCAAAGGAACATGGCCGCCGCGCACAAGACTAACATCAGTGGTAACGAGATTAACTCCGG‑TTGCAGAGCGATAAGACCTAAAACCGACCCCAACCC‑CGAACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAAt                                                                                                                                                            <  1:577098/229‑1 (MQ=255)
                                                                      ttGCCAGCGTCACACCAATCAATAAAC‑CTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTCACTCCAG‑TTGCAGAGCGATAAGACCTGAAATCGAACCAAACAC‑CGTACCCCCACTACGCCCAAAGGCCGGCGGGACAACCTTACCCCAGAACGAGATTGGCCCCACAAccacca                                                                                                                                                       >  2:568769/1‑228 (MQ=255)
                                                                                       caccataaaacctttccgcGGTTTCTCCCCCAGCCCCCACAACCCGAAAAGGAACGTCGCCGCCGCGCACCAGACTAACACCAGCGGTAACGAGATTAACTCCAG‑TGGCAGAGCGAAAAGACCAAAAATCGAACCCAACCC‑CGTACCGCCAATACGCTTAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGc                                                                                                                                      <  2:323108/211‑1 (MQ=255)
                                                                                       atcaatAAAC‑CTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAG‑TTGCAGAGCGATAAGACCTAAAATCGAACCCAACAC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGGGACCAGCGGc                                                                                                                                      >  1:639391/1‑228 (MQ=255)
                                                                                                  cgtgaatcGGTTTCTTGCCCAGCGCCAGCAAACCGAAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACCCCAG‑TGGCAGAGCGATAAGACCTAAAATCGACCCCAACCC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTc                                                                                                                           <  1:486589/222‑1 (MQ=255)
                                                                                                     gATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAG‑TTGCAGAGCGATAAGACCTAAAATCGAACCCAACAC‑CGTCCCGCCAATACGCCCAAAGGCGCGAGGGACAACGTTACCCCAGAACAAAATTGGCCCCATAATCACCCCCAAGGTGACCAGCGGCCAGGGGCATTCCgg                                                                                                                        >  2:617983/1‑229 (MQ=255)
                                                                                                         ctgTTTCTTGCCCAGCGCCAGGCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAG‑TTGCAGAGCGATAAGACCCAAAATCGAACCCAACAC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATa                                                                                                                    <  1:377696/227‑1 (MQ=255)
                                                                                                                  gCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAG‑TAGCAGAGCGATAAGACCTAAAAACGAACCAAACAC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGCCCACATAAGCACCACAATGGAGACCAGCGGCCAGGGGCTTTCCGGGAGAGTAAACAgg                                                                                                           >  2:468874/1‑229 (MQ=255)
                                                                                                                      aGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAG‑TTGCAGAGCGCTAAGACCTAAAATCGAACCCAACAC‑CGTACCGCCCATAAGCTCAAAGGCGCGAAGGCCAACGTTACCCCAGAACGAGATTGGCCCCATAATCAACACCACGGCGACCAGCGTCCAGGGGGTTTTCGGGATAAGAAACacgagaa                                                                                                       >  2:177038/1‑223 (MQ=255)
                                                                                                                        cgcCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAG‑TTGCAGAGCGATAAGACCTAAAATCGAACCCAACAC‑CGTACCGCCAATACGCTCAAAGGCGCGCGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCCCCATGGTGACCCACGGCCAGGGGCTTTCCGGGAAAGTAACCAGgggggaa                                                                                                     >  2:239743/1‑226 (MQ=255)
                                                                                                                             gccaACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAG‑TTGCAGAGCGATAAGACCTAAAATCGAACCCAACAC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCACAACGGGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGAGCTTTCCGGGGAAGTAACCAGACgggaaataag                                                                                                >  1:721194/1‑221 (MQ=255)
                                                                                                                                  ccGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAG‑TTGCAGAGCGATAAGACCTAACATCGAACCCAACAC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGCACAACGTTACCCCAGCACGCGCATGGCCCCATAATCACCACCCTGGTAACCAGCCGGCAGGGGCTTTCCGGGCTAATaaaacagcggagactggagaaag                                                                                           >  2:323347/1‑209 (MQ=255)
                                                                                                                                  ccGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAG‑TTGCAGAGCGATAAGACCTAAAATCGAACCCAACAC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAg                                                                                           >  1:623780/1‑229 (MQ=255)
                                                                                                                                    gaaaaGAAACAGTGCCCGCGCGCACCAAACGAACATGATCTGAAACGAGATT‑GCACCAGTTTGCAAAGCAATAAGACCTAAAACAAACCCCACCACACGT‑CCGGCAATACGCTCAAAGGCGCGAAGGGAAACGTTACCCCATAACGAGATTGGCCCCATAATCACCACCAGGGTGACCAGTGGCGAGGTGGTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTg                                                                                         <  2:442714/227‑1 (MQ=255)
                                                                                                                                                    gccgcGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAG‑TTGCAGAGCGATAAGACCTAAAATCGAACCCAACAC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACAAGCGGCCAGGGGCTTTCCCGGATAGTAAACAGGGGGCAAATGAGAAAAGGGAG‑CAGGAAAGCCaacc                                                                        >  2:543112/1‑226 (MQ=255)
                                                                                                                                                      cgcgccacaagacaaCACCAGCGGTAACGAAATTAACTCCAG‑TTGCAGGGCGATAAGACCTAAAAGCGCACCCAACAC‑GGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAG‑CAGGAATGCCAgcgc                                                                       <  1:428661/215‑1 (MQ=255)
                                                                                                                                                      cgcgCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAG‑TTCCAGAGCGATAAGACCTAAAATCGAACCCAACAC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAG‑CAGGAATGCCAgcgc                                                                       <  1:448398/228‑1 (MQ=255)
                                                                                                                                                      cgcgCAACAGACTAACATCAGCGGAACCGAGATTACCCCCAG‑TTGCAGAGCGATAAGACCTAAACTCGAACCCACCCC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAG‑CAGGAATGCCAgcgc                                                                       <  1:239743/228‑1 (MQ=255)
                                                                                                                                                          gacaggaaaaacaacaagggTAACGAAATTAACTCCAG‑GTGCAGGGCAATAAGACCAAAAATGGAACCCAACCC‑CGTCCCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAG‑CAGGAATGCCAGCGCGAccc                                                                  <  2:748104/211‑1 (MQ=255)
                                                                                                                                                             caaaCTAACATCAGCGGTAACCATATTAACCACAG‑TTTCAGAGCGATAAGCCCTAAAATCGAACCCAACCC‑CTGACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAG‑CAGGAATGCCAGCGCGACCCGa                                                                <  1:663524/225‑1 (MQ=255)
                                                                                                                                                             cAGACTAACATCAGCGATACCGAGATTAACCCCAT‑TTGCAGAGCGATAAGACCTAAAACCGACCCCAACCC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAG‑CAGGAATGCCAGCGCGACCCGa                                                                <  1:113642/228‑1 (MQ=255)
                                                                                                                                                                          gCGGTAACGAGATTAACTCCAG‑TTGCAGAGCGATAAGACCTAAAATCGAACCCAACAC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGCAAAGTGAG‑CAGGAATGCCAGCGCGACCCGAGTACCATGCACAAt                                                  >  1:544824/1‑229 (MQ=255)
                                                                                                                                                                          gCGGTAACGAGATTAACTCCAG‑TTGCAGAGCGATAAGACCTAAAATCGAACCCAACAC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAG‑CAGGAATGCCAGCGCGAccc                                                                  >  1:748105/1‑213 (MQ=255)
                                                                                                                                                                           cGGTAACGAGATTAACTCCAG‑TTGCAGAGCGATAAGCCCTAAAATCGACCCCAACCC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAG‑CAGGAATGCCAGCGCGACCCGAGTACCATGCACaa                                                   <  1:442436/227‑1 (MQ=255)
                                                                                                                                                                              tAACGAGATTAACTCCAG‑TTGCAGAGCGATAAGACCTAAAATCGAACCCAACAC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAGCAGGCGGGTAATTGGAAAAGTGGG‑CCGGAAGTCCAGCAGGACCCGaaaaaacagcacaaagcgg                                              >  2:262686/1‑211 (MQ=255)
                                                                                                                                                                                              aG‑TTGCAGAGCGATAAGACCTAAAATCGAACCCAACAC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGGTAGTAAACAGGCGGATAATGAGAAAAGTGAGCCAGGAATGCCAGCGCAACCCGGGGACCATGCACAAAGCGGTAATGgg‑gggaaaac                              >  2:520995/1‑223 (MQ=255)
                                                                                                                                                                                                         cgaaaaggctcaaaaTCGAACCCAACAC‑CGTACCGCAAATGCGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAG‑CAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGC‑GGTATACCCGGATTTCaaa                   <  1:32503/218‑1 (MQ=255)
                                                                                                                                                                                                                 ccTAAAATCGAACCCAACAC‑CGTACCGCCAATACGCTCAAAAGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGACCAGGTGCTTTCCGGGATAGTAAACCGCCGGTTAATCAGAAAAGTGTG‑CAGGAATGGCAGCGCGCCCCGAGTACCATGCACAATGCGGCAATGGA‑GGTATAACACGAGTTCCAAACGGCTTa           >  2:475354/1‑229 (MQ=255)
                                                                                                                                                                                                                    aaaaTCGAACCCAACAC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAG‑CAGGAATGCCAGAGCGACCCCAGTACCATGCACAATGCGGAAATGGC‑GGGATACCCgcgtttaccaacggggtcaca        >  2:101263/1‑209 (MQ=255)
                                                                                                                                                                                                                           aaCCCAACAC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGCAAAGTGAG‑CAGGAATGCCAGCGCGACCCGAGTCACAAGCACAATGGGGGAATGGCGGGTAAACCGGTATTTAAAAGGGGCTCAAtatttatt  >  2:431378/1‑222 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                              
AGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCG‑GGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAAC‑CTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAG‑TTGCAGAGCGATAAGACCTAAAATCGAACCCAACAC‑CGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAG‑CAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGC‑GGTATACCCGGATTTCAAACGGGCTTAATGACTTAT  >  NC_000913/2080138‑2080581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A