Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F20 I1 R1	21	53.9	1121892	93.4%	1047847	149.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,196,508	C→A	intergenic (+2563/‑1229)	ftsQ → / → ylmE	cell division protein FtsQ/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,196,508	0	C	A	100.0%	127.4 / NA	41	intergenic (+2563/‑1229)	ftsQ/ylmE	cell division protein FtsQ/hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (24/17); total (24/17)

CGTTAATATGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGACGTTCAA  >  CP000730/1196362‑1196651

cGTTAATATGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGATCAAGCGTAAATACTtata                                                                                                                                               <  1:812908/149‑1 (MQ=255)

      tatGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACCACCCAATAGCGCATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATg                                                                                                                                         <  1:491516/149‑1 (MQ=255)

      tatGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCaagg                                                                                                                                         >  1:632806/1‑147 (MQ=255)

      tatGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGAACTGGATTCGGAACAAGCGTAAATACTTATAGCAATg                                                                                                                                         >  1:96242/1‑149 (MQ=255)

            tttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACtt                                                                                                                                   <  1:527771/149‑1 (MQ=255)

             ttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACtta                                                                                                                                  >  1:80705/1‑148 (MQ=255)

                ggTACAGTTATTAATCCTGACTTACAAGATGAGATTGTTGTAAAAGTTATTGCAACTGGTTTTGAGGACAACCCACCATCACATGGTCGTAAATCTGGTAGCAGTGGATTCGGAACAAGCGTAAATACTTATAGCAACCGAACTTCTaa                                                                                                                               >  1:162879/1‑149 (MQ=255)

                  tACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAg                                                                                                                             <  1:984851/149‑1 (MQ=255)

                      gTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATACGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGa                                                                                                                         >  1:209545/1‑149 (MQ=255)

                       ttattaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGaa                                                                                                                        >  1:620354/1‑149 (MQ=255)

                            aaTCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAGTACTTATAGCAATGCAACTTCTAAAGATGAATCAtt                                                                                                                   >  1:998755/1‑149 (MQ=255)

                             aTCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTc                                                                                                                  <  1:331300/149‑1 (MQ=255)

                             aTCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTc                                                                                                                  >  1:621935/1‑149 (MQ=255)

                               ccTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCAc                                                                                                                >  1:348046/1‑149 (MQ=255)

                                 tGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACtt                                                                                                              <  1:690379/149‑1 (MQ=255)

                                   aaTTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTGGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCa                                                                                                            >  1:862766/1‑149 (MQ=255)

                                   aaTTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCa                                                                                                            >  1:635264/1‑149 (MQ=255)

                                               agaTTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAAt                                                                                                >  1:109574/1‑149 (MQ=255)

                                                     ttgtAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACaa                                                                                          >  1:951058/1‑149 (MQ=255)

                                                           cAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAACTACTTATAGCAATGCAACTTCTAAAGATGAATCATTCCCTTCAAATTCCTCCAATGCACAAGCaaat                                                                                    >  1:846652/1‑147 (MQ=255)

                                                           cAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACt                                                                                    >  1:846651/1‑149 (MQ=255)

                                                               tactGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATa                                                                                <  1:710582/146‑1 (MQ=255)

                                                                  ggCAACGGGTTTTGATGACAAACCAACAGCACATGGGCGGAAGGCGGGGAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTGCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAgtg                                                                             <  1:761853/148‑1 (MQ=255)

                                                                    cAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTa                                                                           <  1:423947/149‑1 (MQ=255)

                                                                    aaaCGGGTTTTGATGACAAACCAACAACACATGGTCGTAAATCTGGTAGCCCTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCAATTCACCGATATCAAATGCACAAGCAACTGATAGTGTa                                                                           <  1:271624/148‑1 (MQ=255)

                                                                         ggTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCGTCAAATGCACAAGCAACTGATAGTGTAAGTGa                                                                      <  1:716876/149‑1 (MQ=255)

                                                                          ggtttGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGaa                                                                     <  1:807897/147‑1 (MQ=255)

                                                                           ttttGATGACAAACCAACATCACCAGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAGGTGaaa                                                                    >  1:223924/1‑149 (MQ=255)

                                                                           ttttGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAACGCACAAGCAACTGATAGTGTAAGTGaaa                                                                    <  1:683915/149‑1 (MQ=255)

                                                                             ttGATGAAAAACCAACATCACATGGTCGTAAATCCGGTAGCACCGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGa                                                                  <  1:279359/149‑1 (MQ=255)

                                                                                      aaCCAACATCACATGGTCGTAACACTGGTAGCTGGGGATTCGGCCGGTCCTGGTGTACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACa                                                         <  1:811369/149‑1 (MQ=255)

                                                                                              tCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAaaga                                                 <  1:354453/149‑1 (MQ=255)

                                                                                                    ggTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACCTACAACTAAAGAAgatga                                           <  1:812896/149‑1 (MQ=255)

                                                                                                    ggTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAgatga                                           >  1:625728/1‑149 (MQ=255)

                                                                                                         tAAATCTGGTAGCACTGGATTCGGAACAAGCGTAACTTCTGATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTc                                      >  1:1107885/1‑149 (MQ=255)

                                                                                                                          gATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAAt                     >  1:290232/1‑149 (MQ=255)

                                                                                                                             tCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATaga                  >  1:217834/1‑149 (MQ=255)

                                                                                                                                  aCAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCGAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTGGAAATAGagaaga             >  1:622636/1‑149 (MQ=255)

                                                                                                                                       cGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAACTTCATCCCATGCACAGGAAAGTGATAATGTAAGTAAAAGAACACATACAACGAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGAc        <  1:224249/149‑1 (MQ=255)

                                                                                                                                            aTACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGACGTTCa   >  1:74313/1‑149 (MQ=255)

                                                                                                                                             tACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAGGTAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGACGTGCaa  >  1:31697/1‑149 (MQ=255)

                                                                                                                                             tACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAATACGTTCaa  >  1:506890/1‑149 (MQ=255)

CGTTAATATGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGACGTTCAA  >  CP000730/1196362‑1196651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A