Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A15 F70 I1 R1
|
14 |
80.0 |
1838586 |
92.3% |
1697014 |
136.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP000730 |
2,144,181 |
2 bp→TC |
coding (462‑463/1254 nt) |
sdrH ← |
Ser‑Asp rich fibrinogen/bone sialoprotein bindin protein SdrH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP000730 | 2,144,181 | 0 | C | T | 94.4%
| 28.4
/ NA
| 18 | D155N (GAT→AAT) | sdrH | Ser‑Asp rich fibrinogen/bone sialoprotein bindin protein SdrH |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (11/6); minor base A (1/0); total (12/6) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| * | CP000730 | 2,144,182 | 0 | T | C | 94.4%
| 29.1
/ NA
| 18 | P154P (CCA→CCG) | sdrH | Ser‑Asp rich fibrinogen/bone sialoprotein bindin protein SdrH |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (11/6); minor base A (1/0); total (12/6) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGACGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTT‑‑‑ATTA‑‑‑GGGTCTGGTTTTGGATCTGGATTTGGTTTCGGGTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTATCTGGATCTGGTTTAGGATTCGGACTTGGGTTTTGG > CP000730/2144068‑2144314
||
cgACGGGTTAGGATTTGGCTCATTAGGGCCTGGTTTTGGCTTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATAAGGATTTGGATTCGGGTTata > 2:774597/2‑140 (MQ=11)
ggtgacgggttaggATTTGGCTT‑‑‑ATTA‑‑‑GGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTa > 1:326333/13‑141 (MQ=11)
gacgggttaggATTTGGCTT‑‑‑ATTA‑‑‑GGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTTGGGTTatct > 2:809507/10‑141 (MQ=14)
cgggttaggATTTGGCTT‑‑‑ATTA‑‑‑GGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTatctgg > 1:168999/8‑141 (MQ=14)
gggttaggATTTGGCTT‑‑‑ATTA‑‑‑GGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTatctgga < 2:546520/135‑1 (MQ=14)
aggATTTGGCTT‑‑‑ATTA‑‑‑GGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTatctggatctgg > 1:302086/2‑141 (MQ=14)
ggATTTGGCTT‑‑‑ATTA‑‑‑GGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTATCTGGATCTGGt < 2:722002/141‑1 (MQ=14)
ggATTTGGCTT‑‑‑ATTA‑‑‑GGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTATCTGGATCTGGt < 2:112535/141‑1 (MQ=14)
tttGGCTT‑‑‑ATTA‑‑‑GGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTATCTGGATCTGGTTTa < 1:146674/141‑1 (MQ=14)
ggCTT‑‑‑ATTA‑‑‑GGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTATCTGGATCTGGTTTAGGa > 1:110015/1‑141 (MQ=17)
t‑‑‑atca‑‑‑GGGCCTGGCTTTGGCTTCGGATTTGGCGTTGGTTTATCTGGATTCGGTTTTGGATCCGGATTTGGCTTCGGTTTATCTGGGGCTGGGTTTGGATCCGGTGTTGGTTGCGGGTTATCTGGACCTGGTTTTGGATTCg > 2:744337/1‑141 (MQ=9)
atta‑‑‑GGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTATCTGGATCTGGTTTAGGATTCgg > 2:908325/1‑141 (MQ=17)
a‑‑‑GGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTATCTGGATCTGGTTTAGGATTCGGACt > 1:530991/1‑141 (MQ=17)
a‑‑‑GGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTATCTGGATCTGGTTTAGGATTCGGACt < 1:430260/141‑1 (MQ=17)
gggTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTATCTGGATCTGGTTTAGGATTCGGACtt < 2:588003/141‑1 (MQ=17)
ggTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTATCTGGATCTGGTTTAGGATTCGGACTTg > 1:569769/1‑141 (MQ=17)
tCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTATCTGGATCTGGTTTAGGATTCGGACTTggg > 1:27971/1‑141 (MQ=18)
tttGGATTCGGATTTGGCTATGGTTAATCGGGTGCGGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTATCTGGATCTGGTTTAGGATTCGGACTTGGGTTTTgg > 2:233240/1‑141 (MQ=18)
||
TGACGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTT‑‑‑ATTA‑‑‑GGGTCTGGTTTTGGATCTGGATTTGGTTTCGGGTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTATCTGGATCTGGTTTAGGATTCGGACTTGGGTTTTGG > CP000730/2144068‑2144314
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
