Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F58 I1 R1	11	38.2	843732	93.6%	789733	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	632,919	A→G	G348G (GGA→GGG)	proP →	MFS family major facilitator transporter, proline/betaine:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	632,919	0	A	G	100.0%	152.0 / NA	41	G348G (GGA→GGG)	proP	MFS family major facilitator transporter, proline/betaine:cation symporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/22); total (19/22)

AGCGGATAAAATAGGTGAAAAGAAAGTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGATTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATTTGGTGGTACGACGCCATT  >  CP000730/632787‑633050

aGCGGATAAAATAGGTGAAAAGAAAGTATTTCTAATTGGTACTCGTGGGCTAACATTACTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCtt                                                                                                                             <  1:342255/141‑1 (MQ=255)

 gCGGATAAAATAGGTGAAAAGAAAGTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTa                                                                                                                            >  2:270534/1‑141 (MQ=255)

             ggTGAAAAGAAAGTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGaa                                                                                                                >  2:46157/1‑141 (MQ=255)

                   aagaaagTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACa                                                                                                          >  1:394261/1‑141 (MQ=255)

                     gaaagTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAAt                                                                                                        >  2:56614/1‑141 (MQ=255)

                     gaaagTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAAt                                                                                                        >  1:231451/1‑141 (MQ=255)

                     gaaagTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTAGATTAGGGTTTTTCTTATCAACTTACGAAGCGACAAt                                                                                                        >  1:420229/1‑141 (MQ=255)

                           aTTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAgg                                                                                                  <  1:152472/141‑1 (MQ=255)

                               cTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAGTCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCg                                                                                              <  2:346647/141‑1 (MQ=255)

                               cTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCg                                                                                              >  2:11039/1‑141 (MQ=255)

                                 aaTTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGtt                                                                                            <  1:282700/141‑1 (MQ=255)

                                 aaTTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGtt                                                                                            <  2:387844/141‑1 (MQ=255)

                                 aaTTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGtt                                                                                            <  2:387876/141‑1 (MQ=255)

                                 aaTTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGtt                                                                                            <  2:318139/141‑1 (MQ=255)

                                         cTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGa                                                                                    >  2:404865/1‑141 (MQ=255)

                                            gtggGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGt                                                                                 <  1:382614/141‑1 (MQ=255)

                                            gtggGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGt                                                                                 <  1:51423/141‑1 (MQ=255)

                                              gggCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGttt                                                                               >  1:171617/1‑141 (MQ=255)

                                              gggCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGttt                                                                               <  2:278991/141‑1 (MQ=255)

                                               ggCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGtttt                                                                              <  2:392715/141‑1 (MQ=255)

                                                gCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTa                                                                             <  1:164511/141‑1 (MQ=255)

                                                    aCATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGt                                                                         <  1:365793/141‑1 (MQ=255)

                                                    aCATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGt                                                                         <  2:383922/141‑1 (MQ=255)

                                                           tCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATaa                                                                  >  2:215743/1‑141 (MQ=255)

                                                           tCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATaa                                                                  >  2:266273/1‑141 (MQ=255)

                                                             aGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGa                                                                <  1:235696/141‑1 (MQ=255)

                                                             aGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGa                                                                <  1:165810/141‑1 (MQ=255)

                                                                 tcatcGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATc                                                            <  1:227405/141‑1 (MQ=255)

                                                                              gTTATTACATTCACAATCGTGGTGTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGt                                               <  1:3852/141‑1 (MQ=255)

                                                                              gTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGt                                               <  2:151702/141‑1 (MQ=255)

                                                                              gTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGt                                               <  2:151026/141‑1 (MQ=255)

                                                                                          acaATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACCTTTAatga                                   >  2:121816/1‑139 (MQ=255)

                                                                                           caATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATAtc                                  >  2:97291/1‑141 (MQ=255)

                                                                                           caATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATAtc                                  >  1:271020/1‑141 (MQ=255)

                                                                                                         aaTAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATAtt                    >  2:110913/1‑141 (MQ=255)

                                                                                                             gTAATCGGTATATTTATATTAGGGTTTTTCTTTTTTTCTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATTTTtggt                >  2:351948/1‑141 (MQ=255)

                                                                                                             gTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATTtggt                >  1:353413/1‑141 (MQ=255)

                                                                                                             gTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATAtggt                >  2:396968/1‑141 (MQ=255)

                                                                                                              tAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTATGAAGCGACAATGCCAGGGGCGTTACCTACGATGTTTTACAGTCATATAAGGGATCGAACTTTATAAGTAACATTTAATATCTTTGTTGAGATATTtggtg               >  2:367447/1‑141 (MQ=255)

                                                                                                                        atttatattAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATTTGGTGGTACGACGcc     <  2:171617/141‑1 (MQ=255)

                                                                                                                           tatattAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATTTGGTGGTACGACGCCAtt  <  1:404865/141‑1 (MQ=255)

AGCGGATAAAATAGGTGAAAAGAAAGTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGATTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATTTGGTGGTACGACGCCATT  >  CP000730/632787‑633050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A