Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F70 I1 R2	12	34.5	799746	91.8%	734166	135.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,372,946	G→T	A277D (GCT→GAT)	pbuG ←	NCS2 family nucleobase:cation symporter‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,372,946	0	G	T	100.0%	108.8 / NA	33	A277D (GCT→GAT)	pbuG	NCS2 family nucleobase:cation symporter‑2
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (14/19); total (14/19)

CACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAAT  >  CP000730/2372809‑2373082

cacCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCaa                                                                                                                                       >  1:30633/1‑141 (MQ=255)

   cTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAg                                                                                                                                    <  1:47843/141‑1 (MQ=255)

      aaGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGcta                                                                                                                                 <  2:220746/141‑1 (MQ=255)

             ttCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGt                                                                                                                          >  1:246163/1‑141 (MQ=255)

                   ataAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGc                                                                                                                    <  2:179209/141‑1 (MQ=255)

                       gaagTCGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTa                                                                                                                <  2:322921/141‑1 (MQ=255)

                            tgttgtAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCaaa                                                                                                           <  2:395474/141‑1 (MQ=255)

                            tgttgtAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCaaa                                                                                                           >  1:79972/1‑141 (MQ=255)

                             tttgtAGTTGGTCCAAAAACTGAGCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAg                                                                                                          <  2:33879/140‑1 (MQ=255)

                             gttgtAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAg                                                                                                          <  1:182739/141‑1 (MQ=255)

                             gttgtAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAg                                                                                                          >  1:367732/1‑141 (MQ=255)

                              ttgtAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGa                                                                                                         <  2:54885/141‑1 (MQ=255)

                                 tAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAAt                                                                                                      <  1:94896/141‑1 (MQ=255)

                                                 tGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGt                                                                                      <  2:30633/141‑1 (MQ=255)

                                                  gATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTAGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGtt                                                                                     >  2:30830/1‑141 (MQ=255)

                                                        aCAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATa                                                                               >  1:16555/1‑141 (MQ=255)

                                                          aaTCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAAc                                                                             <  1:21151/141‑1 (MQ=255)

                                                              gTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAAtt                                                                         <  1:89781/141‑1 (MQ=255)

                                                                   tAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGaa                                                                    <  2:200646/141‑1 (MQ=255)

                                                                       gAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGt                                                                <  2:269237/141‑1 (MQ=255)

                                                                       gAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACATGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGt                                                                >  1:7071/1‑141 (MQ=255)

                                                                             gagaACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGtt                                                          <  1:233951/141‑1 (MQ=255)

                                                                                      gCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGa                                                 <  2:248856/141‑1 (MQ=255)

                                                                                         ctACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACtt                                              <  2:246163/141‑1 (MQ=255)

                                                                                                     ggTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAAc                                  <  1:117366/141‑1 (MQ=255)

                                                                                                       tAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGc                                >  1:217457/1‑141 (MQ=255)

                                                                                                                       ataataCCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCg                >  1:118194/1‑141 (MQ=255)

                                                                                                                        taataCCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGa               <  1:120115/141‑1 (MQ=255)

                                                                                                                        taataCCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGa               >  2:296328/1‑141 (MQ=255)

                                                                                                                            aCCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGt           >  2:246302/1‑141 (MQ=255)

                                                                                                                               tGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTgg        >  2:73608/1‑141 (MQ=255)

                                                                                                                               tGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTgg        >  2:73609/1‑141 (MQ=255)

                                                                                                                                     aGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAAt  >  1:284859/1‑141 (MQ=255)

CACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAAT  >  CP000730/2372809‑2373082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A