Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A15 F54 I1 R2
|
10 |
34.7 |
881936 |
84.9% |
748763 |
134.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP000730 |
2,710,898 |
(T)6→7 |
coding (29/1812 nt) |
oatA ← |
possible acetyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP000730 | 2,710,892 | 1 | . | T | 95.7%
| 63.5
/ ‑3.4
| 23 | coding (35/1812 nt) | oatA | possible acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (16/6); total (17/6) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AACCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCTTA > CP000730/2710755‑2711027
|
aaCCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑ttt < 2:319224/141‑1 (MQ=255)
aagaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCGGGTAAGTATCGCGGGGAATACA‑TTTTTTCTAAACGTTTa > 1:68309/1‑141 (MQ=255)
agaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTGAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTa < 1:76137/141‑1 (MQ=255)
tCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCttt < 1:113634/141‑1 (MQ=255)
ccACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGAAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATaaa > 2:376592/1‑141 (MQ=255)
aaCCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGcccc > 2:59604/1‑141 (MQ=255)
aTTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTtc > 2:21079/1‑141 (MQ=255)
tCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTtctc < 1:183190/141‑1 (MQ=255)
gTGATAAATAATGATTCCAATAACTGCGAATGCCCTCACTCCATCTACTCCAGGTAAGTATCGCGGGGAATACATTTTTTTCGAAACGTTTACAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTAtt > 2:262957/1‑141 (MQ=255)
taaataaTGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTAt < 2:398177/101‑1 (MQ=255)
taaataaTGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTAt > 1:398177/1‑101 (MQ=255)
ataatGATTCCTATAACTGCGAATGCCCTCAATCCATCTAGTCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGc > 1:419042/1‑141 (MQ=255)
aTTCCTATAACTGCGAATGCCCTCAATTCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATca > 2:275419/1‑141 (MQ=255)
taACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATAtt > 1:69272/1‑141 (MQ=255)
cGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAATGTTTAAAGTCATTTGTATCCATGTTAATAAACGCCCAATTTATTTTTCTCTATTTTATAGAATTTCACAATATTTTTGaa > 2:103013/1‑141 (MQ=255)
ccATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCa < 2:381811/141‑1 (MQ=255)
aTCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATGTATTTTTCTCTATTTTGTAGCATATCACAATATCTTTGAAAATAAAATATTGCACt < 2:429569/141‑1 (MQ=255)
tCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAACTAAAATATTGCACTg > 2:418007/1‑141 (MQ=255)
cTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGAATATCACAATATTTTTGAAAATAAAATATTGctttga > 1:200462/1‑136 (MQ=255)
tAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGAt > 1:381839/1‑141 (MQ=255)
aaTCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTTTTTTTTTAATATTGCACTGAtt > 2:149851/1‑141 (MQ=255)
aaGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAAt > 1:121015/1‑141 (MQ=255)
gcgGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAAAGCCCCATTTATTATTTACTATTTGGTAGCATAGCACAATATTTTTGAATATAAAATATTGCACTGATTTTCATTAATTGATTTa > 1:237122/1‑141 (MQ=255)
aTACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCtta > 1:171290/1‑141 (MQ=255)
|
AACCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCTTA > CP000730/2710755‑2711027
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
